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Citations for Promega :
9151 - 9200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... quantified with a QuantiFluor dsDNA System (Promega), and pooled equimolarly ...
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bioRxiv - Microbiology 2023Quote: ... then 200 ng trypsin (Promega), diluted in 80 µL of 20 mM ammonium bicarbonate buffer ...
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bioRxiv - Microbiology 2023Quote: ... and DNA was extracted automatically using a Maxwell® Instrument (Promega) into the elution buffer provided in the Maxwell® RSC Blood DNA Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... Putative promoter sequences were digested and ligated into pGL-4.79 [Rluc] (Promega), a Renilla luciferase reporter construct lacking a promoter.
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bioRxiv - Molecular Biology 2023Quote: ... and Asp-N were purchased from Promega (Madison, WI), and a-lytic protease was obtained from New England BioLabs (Ipswich ...
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase assay was performed using the Dual-Glo® Luciferase Assay System (Promega) in triplicate and assay activity was measured with luminescence with the ClarioStar plus spectrophotometer (BMG Labtech).
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bioRxiv - Neuroscience 2023Quote: ... ATP quantification was performed using Cell/Titer-Glo Luminescent Cell Viability Assay Kit (Promega # G7571). Following Aβ40/Aβ42 or ATA treatment in 96 black well plate ...
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bioRxiv - Biochemistry 2023Quote: ... trypsin (Promega) was added at the ratio of 1:75 w/w and the samples were incubated for 6 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... and ligated into the pSiCheck2 plasmid (Promega Cat #C8021) where renilla luciferase is upstream of the 3’UTR and firefly luciferase is driven by a separate promoter as a reference control ...
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bioRxiv - Molecular Biology 2023Quote: ... ATP assays ATP content in freshly isolated islets was measured by the CellTiter-Glo assay (Promega).
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bioRxiv - Neuroscience 2023Quote: ... Transcriptional activity was determined using the Dual Luciferase Assay Kit (Promega). Approximately 48 h after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... pCI-Mrc2 (murine Mrc2 cloned into the pCI-Neo expression vector, from Promega) was used as we previously described18 ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... HRP-conjugated goat anti-rabbit IgG was utilized (Promega). Goat antirabbit antibodies conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Physiology 2023Quote: ... Fluorescence emission was determined at 520 nm using a fluorescence plate reader (GloMax, Promega, USA). Each sample yielded mean fluorescence intensity units that were converted into epinephrine concentration expressed as μg/g adrenal wet weight using calibration standards and polynomial curve fitting.
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bioRxiv - Plant Biology 2023Quote: The cDNA of BdNRT2A was first amplified by PCR using specific forward primer 70F2 and reverse primer 70R2 before being cloned into the pGEMT-easy vector (Promega). Clones with correct sequence were used to produced appropriate PCR product for Gateway cloning ...
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bioRxiv - Plant Biology 2023Quote: ... antibodies followed by anti-rabbit HRP conjugate (Promega, W4018). Protein loading was visualised by staining membranes with Ponceau S and protein signals were developed using the SuperSignal West Femto chemiluminescence kit (Pierce).
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bioRxiv - Plant Biology 2023Quote: ... After in-gel digestion with trypsin (Promega), the resulting peptide mixtures were loaded onto the UltiMate 3000 RSLCnano system ...
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bioRxiv - Plant Biology 2023Quote: ... Lys-C digest was further digested with 0.1 μg trypsin (Promega) with shaking at 1,000 rpm at 37°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... gel pieces were dried and subsequently rehydrated in solution containing 12 ng/μL Trypsin Gold (mass spectrometry grade from Promega), 0.01% ProteaseMAX surfactant (Promega ...
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bioRxiv - Neuroscience 2023Quote: Luciferase-based reporter gene assays were performed using Dual Luciferase Reporter Assay System (Promega, E1910) as described previously (Ma et al. ...
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bioRxiv - Neuroscience 2023Quote: ... We amplified the regions from C57Bl/6 tail snip DNA or from human male genomic DNA (Promega catalog # G1471) using FastPhusion 2x Master Mix (Thermo Fisher catalog # F548L) ...
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bioRxiv - Neuroscience 2023Quote: ... of vt were amplified by PCR from the BAC clone and inserted into the respective NheI and XbaI sites of the luciferase reporter vector pGL4.10 (Promega, Madison, WI). The resultant reporter construct was transiently transfected into COS-7 cells using Lipofectamine LTX (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... 1 x buffer (Promega), 2.5 mM MgCl2 ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time PCR was performed using Gotaq® qPCR mastermix (Promega, Madison, WI) and a CFX 96 real-time system (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... cDNA was produced using GoScript Reverse Transcriptase (Promega, A5004) and a quantitative PCR was performed using SensiFast SYBR (Bioline ...
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bioRxiv - Genomics 2023Quote: Genomic DNA was extracted from 16-day-old seedlings with the Wizard Genomic DNA Purification Kit (Promega), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The reverse transcription was performed using an oligo-dT primer and the ImRrom-II Reverse Transcription System (Promega). The ABI 7700 Sequence Detection System (PE Applied-Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... probes were amplified in triplicates using the SYBR green based GoTaq® qPCR Mastermix (Promega) in a Rotor-Gene Q real-time PCR cycler (Qiagen ...
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bioRxiv - Pathology 2023Quote: ... GoTaq Flexi (PROMEGA, Madison, WI, USA) according to the manufacturer’s protocol and primers targeting MSV-G (MSV-F559bp ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total RNA extract (1 µg) was incubated with random primers (Promega, Madison, WI, USA) at 70°C for 5 min and subsequently cooled on ice ...
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bioRxiv - Neuroscience 2023Quote: Cell viability was determined at DIV 10 using the CellTiter-Blue Cell Viability Assay according to the manufacturer’s instructions (ref: 8080, Promega, Madison, WI). In brief ...
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bioRxiv - Biochemistry 2023Quote: ... digitonin (Promega, #G9441) was added as per the manufacturer’s instructions to a final concentration of 50 ng/mL ...
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bioRxiv - Biochemistry 2023Quote: ... using GoTaq DNA polymerase (Promega), and sequencing at Eurofins Genomic.
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bioRxiv - Biochemistry 2023Quote: ... was added and the mixture was incubated for 30 min at room temperature followed by 30 min incubation with detection reagent (Promega MTase-Glo™ Methyltransferase Assay). Luminescence was detected using a SpectraMax iD5 (Molecular Devices ...
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bioRxiv - Biochemistry 2023Quote: ... or elastase (V1891, Promega) was further added according to the instruction of manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... For trypsin digestion 1 μg of trypsin (V5111; Promega) was added ...
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bioRxiv - Biochemistry 2023Quote: ... In-gel digestion was performed with trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega, Madison ...
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bioRxiv - Bioengineering 2023Quote: ... lysis reagent (Promega) was added to the cell pellet and then the pellet debris was removed by centrifugation ...
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bioRxiv - Cancer Biology 2023Quote: ... The second half of the slices were stained for apoptosis assessment according to the DeadEnd™ Fluorometric TUNEL System (Promega, Cat. no. G3250) protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... before trypsin (Promega) was added (20:1 protein:enzyme ratio ...
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bioRxiv - Cancer Biology 2023Quote: KIF18A motor activity was measured using an ADP-Glo luminescence assay (Promega, V9101). Pig microtubules (60μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... and the GloMax® Discover Microplate Reader (Promega). IC50 values were calculated using non-linear regression of log-transformed ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μL culture were added to 45 μL NanoGlo reagent (1x Promega lysis buffer and NanoGlo reagent; Promega). The average luminescence of the background cultures was subtracted to the average luminescence of each sample.
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bioRxiv - Cell Biology 2023Quote: ... The proteins were resuspended in 100 mM NH4HCO3 and were digested with trypsin (Promega, UK) at an enzyme-to-substrate ratio of 1:25 ...
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bioRxiv - Cell Biology 2023Quote: ... 5% glycerol) and Ndc80c was fluorescently labeled with HaloTag TMR ligand (Promega) for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The wild-type and mutants of MCHR1 and MCHR2 were constructed into the pcDNA6.0 vector (Promega) for functional assays.
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bioRxiv - Molecular Biology 2023Quote: ... cell proliferation was measured using CellTiter 96 AQueous One Solution Cell Proliferation Assay (G3582, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Bio-GloTM Reagent (Promega) was prepared according to the manufacturer’s manual and added to each well at 80 µL per well ...
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bioRxiv - Molecular Biology 2023Quote: ... the CellTiter-Blue® Cell viability Assay (Promega) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg of Turbo DNase treated total RNA was incubated with or without 25 U RNase One (Promega) and 25 μg RNaseA (Thermo Scientific ...