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Citations for Promega :
9451 - 9500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Reagents used to analyze Luciferase activity were from the Dual-Glo Luciferase Assay System (Promega, E2940) according to the supplier’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and cloned into the pGEM-T easy vector (Promega, Madison, WI, USA) according to instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and luciferase activities of cell lysate were recorded by using a dual-luciferase reporter assay system (Promega).
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bioRxiv - Neuroscience 2023Quote: ... 5 mM DTT with sequencing-grade trypsin (Promega) for one hour at room temperature with 1:800 or 1:1600 mass (w/w ...
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bioRxiv - Genomics 2023Quote: ... 500 μl of CTAB lysis buffer and 10 μl of Proteinase K (20 mg/ml; Promega) were added to the sample and incubated at 65ºC for 90 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... cDNAs were synthesized from 500 ng of total RNA for each sample using M-MLV reverse transcriptase (Promega, M1701) and (dT)25 according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA was extracted using a PolyATract kit (Promega) and was reverse transcribed using Superscript III (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... FuGene HD (Promega) was used for transfection of the plasmids using 3 μg DNA (1.35 μg pCMV delta8.91 ...
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bioRxiv - Cancer Biology 2023Quote: ... 24h after transfections cells were lysed using 50μl passive lysis buffer (Promega) per 105 cells and flash frozen/thawed two times in liquid nitrogen and a 37°C water bath ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription of RNA was performed with the Reverse Transcription System (A3500, Promega), and formed cDNA from 1 μg total RNA ...
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bioRxiv - Immunology 2023Quote: ... Mass Spectrometry Grade (Promega) or endoproteinase AspN (New England BioLabs ...
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bioRxiv - Immunology 2023Quote: ... The dual luciferase assay kit was obtained from Promega, and the firefly and Renilla luciferase activities were measured in cell lysates using a luminometer ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated using an automated magnetic bead purification method (Hamilton Microlab STAR liquid handling machine and Promega ReliaPrep Large Volume HT gDNA isolation system ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated for 72 h before assaying by OneGlo luciferase assay reagent (Promega). All plates included 8 wells of negative control siRNA (Ambion Silencer Select Negative Control #2) ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were collected at day 6 and measured by CellTiter-Glo (Promega #PR-G7573). Cell viability values were analyzed following blank cell deductions and normalization to vehicle readings ...
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bioRxiv - Neuroscience 2023Quote: ... 0.01% Digitonin (Promega, catalog nr G9441)) and incubated on ice for 3min ...
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bioRxiv - Neuroscience 2023Quote: ... followed by kinase assays run using the ADP-Glo Kinase Assay kit (Promega). After the termination of kinase assay ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase activity was measured using the Dual-Luciferase Assay System (Promega, #1910).
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured with Cell Titer Glo 3D (Promega cat.no G9683) and luminescence was measured using a Tecan infinite M200 plate reader ...
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bioRxiv - Cancer Biology 2023Quote: Reagents and protocol were purchased from CellTiter-Glo® Luminescent Cell Viability Assay (Promega). We conducted Cell-titre-glo (CTG ...
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bioRxiv - Cancer Biology 2023Quote: Apoptosis was assessed using the luminescence-base Caspase-Glo 3/7 assay kit (Promega) and defined by at least a 1.5-fold increase in signal activation concerning controls ...
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bioRxiv - Cancer Biology 2023Quote: gDNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue sections was carried out using Maxwell 16 FFPE plus LEV DNA purification kit (AS1720; Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: We tested the functionality of our purified NF1 by evaluating its RAS-GAP activity through the GTPase-Glo™ assay (Promega), a bioluminescent assay that quantitates the amount of GTP remaining after a GTPase reaction ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 μL of CellTiterGlo reagent (Promega) was added to each well and luminescence was quantified on an Envision MultiLabel Plate Reader (PerkinElmer) ...
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bioRxiv - Cell Biology 2023Quote: ... 10mM Ultrapure ATP and 0.7 μg of recombinant full-length human CDC7/DBF4 kinase (Promega, V5088). Reactions were incubated at 37 °C for 120 min and then terminated by freezing on dry ice before mass spectrometry.
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bioRxiv - Cancer Biology 2023Quote: ... CellTiter-Glo assay (Promega) was performed and luminescent signals measured by an Envision plate reader (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell growth was assessed using CellTiter-Glo Luminescent Cell Viability Assay (Promega). All cell viability data was collected on an Envision luminescence microplate reader and calculated for at least 6 replicates per time point ...
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bioRxiv - Molecular Biology 2023Quote: ... and 24 hours later co-transfected with 500 ng psiCHECK™-2 Vector (Promega) containing the 3’UTR of human PLIN2 gene downstream to the Renilla luciferase gene ...
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bioRxiv - Cancer Biology 2023Quote: ... and cDNA was prepared as per the GoScript™ Reverse Transcription System (Promega) protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... QuantiFluor ® dsDNA System (Promega Corp.), and Kapa Illumina Library Quantification qPCR assays (Kapa Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... 2x GoGreen Master Mix (Promega) was used for these reactions ...
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bioRxiv - Immunology 2023Quote: ... and digested twice with trypsin (Promega VA9000) at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μL of buffer containing 35 mM iodoacetamide and Trypsin/Lys-C (Promega, V5072) was added to each sample ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of FgSte3-NLuc donor protein produced was also validated performing immunoblotting using anti-Nluc antibody (Promega cat#7000), see details below.
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were mini-prepped (Promega, A1222) and validated by restriction enzyme digestion and Sanger sequencing.
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bioRxiv - Immunology 2023Quote: ... the infectivity of the pseudotyped viruses was determined by measuring the luciferase activities using a PicaGene Luminescence Kit (Fujifilm Wako, Osaka, Japan) with a GloMax Navigator Microplate Luminometer (Promega, WI, USA).
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bioRxiv - Biochemistry 2023Quote: ... The efficiency of biotinylation was subsequently evaluated by capture using streptavidin-coated paramagnetic particles (Promega). The first round of selection was performed using 400 nM of nanodisc-reconstituted MmFLVCR2 diluted in selection buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: M-MLV buffer (Promega), 1 μL 0.1M DTT ...
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bioRxiv - Biochemistry 2023Quote: HEK-293 stably expressing the Glosensor cAMP reporter (Promega Corp.)21 and epitope-tagged NK1R were seeded in a black-walled 96-well plate flat clear bottom (Corning ...
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bioRxiv - Biochemistry 2023Quote: ... NanoBRET NanoGlo Substrate + Extracellular NanoLuc Inhibitor (Promega, N2540) was added as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: Cells stably expressing epitope-tagged NK1R were transiently transfected (Lipofectamine™ 3000 Transfection Reagent) with 1 μg of pGL4.29[luc2P/CRE/Hygro] or pGL4.30[luc2P/NFAT-RE/Hygro] plasmids (Promega Corp.), seeded in a white-walled 96-well flat clear bottom plate (Corning #3610) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated for 24hr at 37°C and cell death was measured through a lactate dehydrogenase (LDH) assay using the CytoTox 96 kit (Promega). 50 μL of media was collected from all experimental wells with two replicates each and placed in a fresh 96-well plate ...
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bioRxiv - Biochemistry 2023Quote: Kinase assays were conducted using an ADP-Glo™ Kinase Assay Kit (Promega). Reagents were prepared as described in the kit ...
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bioRxiv - Cancer Biology 2023Quote: ... A CellTiter-Glo® Luminescent Cell Viability Assay (Promega Corporation, Madison, WI, USA) was carried out according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... and 100 nM JF-549 HaloTag ligand (Promega) before the column binding step ...
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bioRxiv - Cancer Biology 2023Quote: ... Fluorescence intensity (excitation at 540 nm, emission at 560 nm) was measured using a GloMax-96 Microplate Luminometer (Promega, Madison, WI, USA) on different days ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor-unmatched normal variant calling was performed with a pool of sex-matched DNA (Promega, Madison, WI) using Strelka (v.2.4.7) ...
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bioRxiv - Cell Biology 2023Quote: ... 6 µg total RNA was treated with DNase RQ1 (Promega), purified with RNA Clean & Concentrator-5 kit (Zymo Research ...
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bioRxiv - Cell Biology 2023Quote: ... modified porcine trypsin (Promega), following reduction with 1.5 mg ml-1 dithioerythritol and alkylation with 9.5 mg mL-1 iodoacetamide ...
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bioRxiv - Genetics 2023Quote: Restriction enzyme digestion with EcoRI (Promega, Madison, U.S.A) was performed by placing 15.3μl of sterile RO water in an Eppendorf ...