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Citations for Promega :
8301 - 8350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... the Tunnel staining kit (Promega) was used according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... the DNA concentration per individual was measured using a Quantus fluorometer (Promega, USA). We sequenced a pool of 7–28 individuals (S1Table ...
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bioRxiv - Microbiology 2023Quote: The CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega) was used to determine the cytotoxicity of clovibactin on NIH 3T3 mouse embryonic fibroblast (ATCC CRL-1658 ...
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bioRxiv - Microbiology 2023Quote: ... and 10 U of RQ1 RNase-free DNase (Promega) was added and RNA purified using the GTC-phenol:chloroform extraction procedure as previously described for S ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was run on a 2% agarose gel using gel electrophoresis and the band was purified with the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, USA). Samples were submitted to Macrogen Europe (Amsterdam ...
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bioRxiv - Microbiology 2023Quote: ... using the Maxwell RSC® DNA FFPE kit (Promega, Madison, WI, USA). DNA concentration was measured with the Qubit® dsDNA HS Assay Kit (Thermo Fisher Scientic ...
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bioRxiv - Microbiology 2023Quote: DNA extractions for genomic sequencing were performed using Maxwell™ RSC Cell DNA Purification Kit and automated extraction on the Maxwell RSC 48 Instrument (Promega). The DNA concentration was evaluated using a Qubit dsDNA BR assay kit and Qubit Fluorometer (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: Promoter constructs were created by cloning the immediate 4.5-kb region adjacent to the 5’ TSS of SPINK7 into the promoterless Nano-luciferase reporter vector pNL1.1-NL (Promega). The 4.5-kb sequence and subsequent constructs were created by using primers with the restriction enzyme sites KpnI-HF and XhoI ...
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bioRxiv - Immunology 2023Quote: HIC1 binding on RUNX1 promoter was assessed using Dual-Luciferase® Reporter Assay by cloning the ChIP-defined genomic region upstream of a minimal promoter driving a luciferase gene (pGL4.10 [luc2/minP]; Promega). Wild type HIC1 binding motif of the RUNX1 proximal promoter (5′-TGCCCTGG-3′ ...
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bioRxiv - Immunology 2023Quote: ... RNA extracted was treated with RNase-free DNase (Promega Catalog: M6101) to remove genomic DNA contamination ...
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bioRxiv - Immunology 2023Quote: ... and the corresponding SARS-CoV-2 spike construct were co-transfected in HEK293-T cells using FuGENE 6 Transfection Reagent (Promega) in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Microbiology 2023Quote: ... Transient transfections were performed the following day by lipofection using FuGENETM HD (Promega, #E2311) as instructed by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... 20 μL of MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) inner salt (Promega) was added to each well and the plate was further incubated for 1 h at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... plasmids were prepped from the cell using a Wizard® Plus SV Minipreps DNA Purification kit (Promega). The entire plasmid sequence was obtained using the company Plasmidsaurus ...
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bioRxiv - Microbiology 2023Quote: ... A total of 5 U of recombinant RNasin (Promega) and 10 U of RQ1 RNase-free DNase (Promega ...
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bioRxiv - Immunology 2023Quote: We performed a Cbl-b cellular thermal shift assay (CETSA) using NanoLuc split luciferase technology (Promega). HEK293T cells were plated in 6-well plates (4e5 cells/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 37° C with 1 μg mass spectrometry grade Trypsin (Promega). The resulting peptide sample was acidified with 10% formic acid and 10% trifluoroacetic acid (TFA ...
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bioRxiv - Microbiology 2023Quote: ... Trypsin was reconstituted in 10 mM CaCl2 (sequencing grade, Promega, Middleton, WI) and added at a 1:40 enzyme to total protein ratio ...
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bioRxiv - Microbiology 2023Quote: ... which is detected using the AMP-Glo assay kit (Promega, V5011). The activity of the recombinantly expressed NUDT5 with ADP-ribose was determined by serially diluting NUDT5 from 2 to 0.002 μM using PARP10 reaction buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescence was quantified using a Glomax Multi Detection Plate Reader (Promega).
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bioRxiv - Neuroscience 2023Quote: ... and RNAse Inhibitor (Promega, Cat. No. N2615) with 10 strokes ...
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bioRxiv - Neuroscience 2023Quote: ... Trypsin (Sequencing Grade Modified Trypsin; Promega) was added in a 1:50 w/w ratio of enzyme:total protein and incubated overnight at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Inserts (primers and size are presented in Supplementary Table S5) were cloned into pGEM-T Easy (Promega, Madison, WI) vector and confirmed by restriction and sequencing analysis ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were digested by trypsin (Promega, Madison, WI, USA), and peptides were extracted from the gel with an acetonitrile/formic acid solution ...
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bioRxiv - Neuroscience 2023Quote: HEK293T cells were transfected with either Tau-encoding or control constructs using Fugene 6 (Promega, cat. no. E2691) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Luciferase reporter assay was performed according to the manufacturer’s protocol using the Dual Luciferase Reporter Assay System (Promega, E1910). For transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... 20U/ml Rnase inhibitor (Rnasin, Promega), 1X Protease inhibitor cocktail (cOmplete ...
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bioRxiv - Neuroscience 2023Quote: ... before DNA extraction using the Wizard SV genomic DNA extraction kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... mAb anti-ý-Galactosidase (1:1000, Promega), mAb anti-Calcineurin (ý-subunit ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1U/µl RNAse inhibitor (Promega RNasin Ribonuclease Inhibitor N2615), and 0.02U/µl DNAse (D4527 ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated with ApoTox-GloTM Triplex Assay (Promega, #G6320) according to manufactures recommendation ...
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bioRxiv - Microbiology 2023Quote: ... Standard calibration curves were performed using a 10-fold serial dilution (103 to 108 molecules) of standard pGEM-T Easy plasmids (Promega, Madison, WI, USA) containing target sequences from Escherichia coli K12 ...
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bioRxiv - Molecular Biology 2023Quote: ... coli S30 Extract System for Linear Templates (Promega), according to the manufacturer’s instructions for radiolabelled translation ...
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bioRxiv - Developmental Biology 2023Quote: ... a minimum of 1 µg of RNA was combined with 1 µl of Oligo (dT)15 primer (Promega, C110B) and nuclease-free water to make up a total of 5 µl and incubated at 70°C and 4°C for 5 min each ...
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bioRxiv - Genomics 2023Quote: ... using the Maxwell 16 (Promega) utilizing magnetic nickel beads in a semi-automated nickel affinity purification strategy ...
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bioRxiv - Genomics 2023Quote: ... Protein was precipitated by the addition of 200 μl Protein Precipitation Solution (Promega Wizard HMW DNA Extraction Kit A2920). Using a wide bore tip the samples were mixed by drawing up contents from the bottom of the tube and then expelled on the side of the tube 5 times ...
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bioRxiv - Genomics 2023Quote: ... 500 μl of HMW Lysis Buffer A (Promega Wizard HMW DNA Extraction Kit A2920) and 3 μl RNAse A (ThermoFisher EN0531 ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies were revealed using species-appropriate HRP-conjugated secondary antibodies (anti-rabbit or anti-mouse IgG (1:5000, Promega), or anti-guinea pig IgG (1:5000 ...
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bioRxiv - Genomics 2023Quote: ... GSCs were validated by Short Tandem Repeat (STR) DNA fingerprinting using nine highly polymorphic STR loci plus amelogenin (Cell ID™ System, Promega Inc). All GSC profiles were queried in public databases to confirm authenticity (Visconti et al. ...
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bioRxiv - Genomics 2023Quote: ... The total cellular DNAs of the activated CD4+ T cells were isolated using a DNA extraction kit (Promega Wizard) for analysis.
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bioRxiv - Genomics 2023Quote: ... 1U/μL Recombinant RNase inhibitor (Promega, PAN2515), and 0.1% Triton X-100) ...
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bioRxiv - Genomics 2023Quote: ... DNA extraction from pre-enrichments and selective enrichments was done using the Promega Maxwell® RSC cultured cells DNA kit (Promega, WI, USA, AS1620) according to the manufacturer’s specifications on the Promega Maxwell® RSC 48 Instrument ...
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bioRxiv - Genomics 2023Quote: ... leaves and fruits were collected and in the field and total RNA was extracted with Maxwell® RSC Plant RNA Kit (Promega). Two pools were generated and used for PacBio Iso-Seq library preparation (Procedure & Checklist – Iso-Seq™ Express Template Preparation for Sequel® and Sequel II Systems ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5 µl Recombinant RNasin® Ribonuclease Inhibitor (Promega, N251A), 1 µl Reverse Transcriptase (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 dNTP mix (Promega, C114B), 0.5 µl Recombinant RNasin® Ribonuclease Inhibitor (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... 2.4 µl MgCl2 (Promega, A351H), 1 dNTP mix (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 µl Reverse Transcriptase (Promega, M314A). Thermal cycling was as follows ...
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bioRxiv - Developmental Biology 2023Quote: ... 4 µl 5x reaction buffer (Promega, M289A), 2.4 µl MgCl2 (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... The ImProm-II™ Reverse Transcription System (Promega) was used for cDNA synthesis ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA was eliminated with DNase I (Promega) and RNA probes were purified using Micro Bio-Spin columns (Bio-RAD) ...