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Citations for Promega :
8251 - 8300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Retroviral producer cell lines were generated by transiently transfecting of H29 cells with named constructs utilizing standard CaPO4 transfection (Promega, Madison, WI, USA). Supernatant from transfected H29 cells was used to transduce Phoenix-ECO (pECO ...
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bioRxiv - Immunology 2023Quote: T-cell and prodrug cytotoxicity assays with secreted enzymes were performed after 24 to 48 hours using CellTiter-Glo (Promega, Madison, Wisconsin, USA) or luminescence using firefly (ffLuc ...
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bioRxiv - Evolutionary Biology 2023Quote: ... one microliter volumes of Reverse Transcription reactions were used as template for amplification of internal cDNA fragments using in 20 μl volume PCR reactions (35 rounds of amplification using Promega 2X PCR Master Mix (Promega, Madison, WI). Gene-specific primers and annealing temperatures are listed in Table 1 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... post-injection using a Synergy HTX Multi-mode Microplate Reader (Bio Tek Instruments, Winooski, VT) and the Nano-Glo Dual Luciferase Reporter System (Promega, Madison, WI) as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and digested with trypsin/Lys-C mix (V5071, Promega, Madison, WI). The resulting peptides were cleaned up with Acclaim™ PepMap™ 100 C18 spin columns (SEM SS18V ...
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bioRxiv - Developmental Biology 2023Quote: ... and transcription with T7 RNA polymerase (Promega, Madison, WI).
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bioRxiv - Genetics 2023Quote: ... 36 hours after transfection firefly and renilla luciferase activities were measured using the Dual- Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... The firefly luciferase constructs were co-transfected with the Renilla luciferase vector (Promega) in 1-2X10^6 LCLs at a ratio of 2ug:40ng respectively ...
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bioRxiv - Genetics 2023Quote: ... The assay was performed on fresh leaf disks collected 72 hours after Agrobacterium-mediated infection and following the manufacturers instruction (Promega). A reference vector overexpressing the Renilla reniformis luciferase (REN ...
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bioRxiv - Microbiology 2023Quote: ... phage genome was extracted using Wizard genomic DNA purification kit (Promega, Madison, WI) and sequenced at Massachusets General Hospital next generation sequencing core facility ...
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bioRxiv - Microbiology 2023Quote: ... in the presence of ProteaseMax (Promega) overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were incubated in freshly made stain solution (5mM potassium ferricyanide + 5mM potassium ferrocyanide + 1mg/mL X-gal (Promega cat no. V3941) in wash buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... then incubated in freshly made stain solution (5mM potassium ferricyanide + 5mM potassium ferrocyanide + 1mg/mL X-gal (Promega cat no. V3941) in wash buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and 1μg total DNA:4μL of Fugene (Promega) per well ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... GoTaq qPCR master mix (Promega A6001) was used in a final reaction volume of 20 µl ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-beta galactosidase (Promega Z378825580610; 1:500), guinea pig anti-brk (provided by from Gines Morata ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Multiplex PCRs were modified using 5X GoTaq Flexi colorless reaction buffer (Promega Corp., Madison, USA) instead of 1X and performed using a T100™ Thermal Cycler (Bio-Rad Laboratories Inc.).
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bioRxiv - Evolutionary Biology 2023Quote: ... Amplicons were cloned into the pGEM-T vector (Promega, USA) and their identity confirmed using Sanger sequencing ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Finally embryos were placed in BCIP-NBT (Promega) with AP buffer to react ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Probe synthesis reactions were performed at 37 °C for 3 h and then were treated with DNase I (Promega) at 37 °C for 20 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Levels of reporter mRNAs in eggs were measured 6 hrs post-injection by RT-qPCR using the GoTaq 2-Step RT-qPCR system as per manufacturer’s instructions (Promega, Madison, WI) with random primers for reverse transcription and oligos listed in Table 1 for the qPCR step.
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bioRxiv - Evolutionary Biology 2023Quote: ... The identity of PCR products was verified by Sanger sequencing of amplicons cloned using the pGEM-T cloning system (Promega, Madison, WI) as per manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... and random hexamer primers (Promega). PCR was carried out by QuantStudio-3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2023Quote: ... embryos were rehydrated and treated with Proteinase K (Promega; V3021). Following re-fixation and prehybridization ...
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bioRxiv - Genetics 2023Quote: ... the vector was used as a template for in vitro transcription using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Cat#P1320). The synthesized sgRNAs were extracted with phenol (pH 4–5):chloroform:isoamyl alcohol (125:24:1 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the bands were excised from the gel and TA cloned using the pGEM- T easy-vector II cloning kit (Promega). The standard mini-prep step was avoided by using colony PCR ...
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bioRxiv - Genetics 2023Quote: ... RNA (1000 ng input) was subsequently reverse-transcribed to cDNA using random primers and the GoScript RT kit (Promega). RT-qPCR was performed using FIREPoly qPCR Master Mix (Solis BioDyne ...
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bioRxiv - Microbiology 2023Quote: ... Cleaned libraries were then sized-checked with an Agilent Technology 2100 Bioanalyzer using a High sensitivity DNA chip and quantified by Promega Quantus™ fluorometer using OneDNA protocol ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative PCR was performed using GoTaq qPCR Master Mix (Promega) and primers shown in S3 Table ...
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bioRxiv - Microbiology 2023Quote: ... diluted with 40 μL PBS and assayed for secreted gaussia Luciferase (gLuc) activity by injecting 30 μL coelenterazine solution (Promega) followed by 1.6 sec incubation period and a luminescence integration read time of 1.0 sec ...
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bioRxiv - Microbiology 2023Quote: ... following the manufacturer’s protocol (Promega).
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bioRxiv - Microbiology 2023Quote: ... (nLuc, Promega), 250 ng pmCherry-C1 plasmid ...
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bioRxiv - Microbiology 2023Quote: ... cleaned and ligated using T4 DNA ligase (Promega). Ligation was transformed into chemically competent TOP10 E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in which proteins were reduced (DTT) and alkylated (IAA) before the digestion with Trypsin and Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade Promega) for 14 hours at 37° C ...
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bioRxiv - Microbiology 2023Quote: ... followed by an overnight trypsin digestion (Promega) at a ratio 1/100 (enzyme/proteins) ...
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bioRxiv - Microbiology 2023Quote: ... GoTaq® Green Master Mix (Promega) was used following the manufacturer’s protocol to prepare a 20 µL reaction mix containing 0.5 µM of each primer and 5 µL of template DNA ...
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bioRxiv - Developmental Biology 2023Quote: ... T7 or SP6 RNA polymerases (Promega) and digoxigenin or fluorescein labeled nucleotides (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... the corresponding promoter regions of the human KLF2 and KLF4 genes were amplified using human genomic DNA as a template and cloned into the BglII site of the GL4 basic vector (Promega, Fitchburg, WI). Point mutants of the vectors were constructed by site-directed mutagenesis using a QuikChange Site-Directed Mutagenesis kit (Stratagene ...
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bioRxiv - Cell Biology 2023Quote: ... China and listed in Table S2) were incubated with 201tμL of the real-time PCR reaction mixture including GoTaq qPCR Master Mix (Promega, USA) at 95°C for 3 min ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with plasmid DNA by the calcium phosphate or lipofection method using FuGene 6 (Promega, WI) (Yoshida et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Top half was incubated in anti-Nup107 (1:1000; ab236634) and HaloTag (1:500; Promega G9211). Bottom half was incubated in anti-GAPDH (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... with the transfection reagent of Fugene HD (Promega, E2311). 72 hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: HeLa FlpIn were co-transfected with pCDNA5 constructs with gene of interest and construct of pOG44 recombinase in a 1:9 ratio with Fugene HD (Promega, E2311) under the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... or pCEP4-OCT4-T234A-S235A (addgene, 40630) or pCEP4-OCT4-T234E-S235E (addgene, 40631) for 48 h using FuGENE 6 (Promega, E2691) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... using Pfu polymerase (Promega). Mammalian expression constructs were made using pcDNA5/FRT/TO (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1 U/µL RNasin (Promega; Cat# N2111) and 50 µg/mL cycloheximide ...
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bioRxiv - Cell Biology 2023Quote: ... All cells were transfected using FuGENE HD (Promega, E2311) and imaged 16-24 hours after transfection.
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bioRxiv - Immunology 2023Quote: ... The components were mixed with 30 μl FuGene (Promega) in 250 Opti-MEM and incubated for 5 min at RT ...
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bioRxiv - Immunology 2023Quote: ... dNTP Mix and Oligo (dT)15 Primer (Promega), Low Input Quick Amp Labeling Kit ...
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bioRxiv - Immunology 2023Quote: ... after which cells were collected and luciferase assay was performed using the Dual Luciferase Reagents (Promega). Firefly luciferase activity was normalized to renilla luciferase activity for each sample and expressed as fold change over empty pGL4-minP ...