Labshake search
Citations for Promega :
5251 - 5300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... Japan) at 37 C for 2 h before digestion overnight with 1 ug Trypsin/Lys-C mix (V5071, Promega, Madison, WI, USA) at 37 C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Full-length GID4 N or C-terminal NanoLuc-fusion was transfected into HEK293T cells using FuGENE HD (Promega, E2312) according to manufacturer’s instructions and proteins were allowed to express for 20 hours ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were washed three times with PBSTR (PBS containing 0.1% Tween-20 (Promega, 0000395426), 0.1 U/µL RRI (Vazyme ...
-
bioRxiv - Molecular Biology 2023Quote: ... Luciferase assays were conducted using the Dual-Luciferase Reporter Assay System (Promega). HeLa cells were seeded at 70,000 cells per well of a 24-well plate approximately 24 hours prior to transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... and another 10 μl the NanoLuc substrate (furimazine, 1:25 dilution, Promega) diluted in the detection buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... the proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described71 ...
-
bioRxiv - Molecular Biology 2023Quote: ... or empty vector control (400ng) with 2.4 ul Fugene HD (Promega, E2311) in 43 ul Opti-MEM media (Gibco) ...
-
bioRxiv - Molecular Biology 2023Quote: The human MEOX1 enhancer syntenic to the mouse Meox1 peak9/10 enhancer3 fragment along with the mouse Il1b peak5 and peak6 fragments were PCR amplified and cloned into the XhoI and HindIII sites in the Firefly luciferase plasmid pGL4.23 (Promega) using the Cold Fusion kit (System Biosciences Inc ...
-
bioRxiv - Molecular Biology 2023Quote: The pGL3 vector-based constructs (Promega, E1751) described earlier (17 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the SV40pro+SV40e (Promega, E1741) vectors were used to evaluate promoter and enhancer activity ...
-
bioRxiv - Molecular Biology 2023Quote: ... gene activity was assessed according to the supplied Luciferase Assay System Kit protocol (Promega). Luminescence detection was performed using the Microlumat LB96p device (EGG Berthold ...
-
bioRxiv - Molecular Biology 2023Quote: ... the SV40pro (Promega, E1761) and the SV40pro+SV40e (Promega ...
-
bioRxiv - Molecular Biology 2023Quote: ... Gel slices were pre-incubated with 20 μl of pre-activated trypsin (Promega France, Charbonnières) at room temperature for 15-20 min ...
-
bioRxiv - Molecular Biology 2023Quote: The synthesis of double-stranded RNA (dsRNA) was followed by the manufacturer’s instructions of the T7 RiboMAXTM Express RNAi System (Promega, USA). Briefly ...
-
bioRxiv - Molecular Biology 2023Quote: ... digested with SacI (Promega) and separated by electrophoresis in 0.8% agarose (Methods S1) ...
-
bioRxiv - Molecular Biology 2023Quote: ... provided by the Nano-Glo® HiBiT Blotting System (PROMEGA N2410). Readout was carried out in a ChemiDoc XRS+ (BioRad).
-
bioRxiv - Molecular Biology 2023Quote: ... prepared following the FuGENE HD (PROMEGA) protocol was added to 50 μl of seeded cells ...
-
bioRxiv - Molecular Biology 2023Quote: PROTAC toxicity was determined by using the CellTiter-Glo® 2.0 Cell Viability Assay (PROMEGA G9241). For this ...
-
bioRxiv - Molecular Biology 2023Quote: Full-length VHL was obtained as plasmids cloned in frame with a terminal NanoLuc-fusion (Promega, ND2700). The plasmid was transfected into HEK293T cells using FuGENE HD (Promega ...
-
bioRxiv - Molecular Biology 2023Quote: ... using the Nano-Glo® HiBiT Lytic Detection System (PROMEGA N3030) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... Serially diluted inhibitor and NanoBRET CRBN Tracer (Promega) at a concentration previously determined (1 μM ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 ml FuGENE HD (Promega, E2312) transfection mix containing 30 μl FuGENE HD and 8 μl of each plasmid was prepared per plate ...
-
bioRxiv - Molecular Biology 2023Quote: ... After incubation at 37 °C/5 % CO2 for 20 h 40 nl HaloTag® NanoBRET™ 618 Ligand (PROMEGA) was added to the cells using an Echo acoustic dispenser (Labcyte ...
-
bioRxiv - Molecular Biology 2023Quote: ... NanoBRET NanoGlo Substrate + Extracellular NanoLuc Inhibitor (Promega, N2540) were added as per the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... using the Nano-Glo® HiBiT Blotting System (PROMEGA N2410). Here ...
-
bioRxiv - Molecular Biology 2023Quote: ... The plasmid was transfected into HEK293T cells using FuGENE HD (Promega, E2312) and proteins were allowed to express for 20h ...
-
bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
-
bioRxiv - Molecular Biology 2023Quote: ... The 3,199-bp pGEM3 Zf(+) was used as a source of circular ssDNA and dsDNA (Promega Biotech, Spain). DNA concentrations were established using the molar extinction coefficients of 8780 and 6500 M-1 cm-1 at 260 nm for ssDNA and dsDNA ...
-
bioRxiv - Molecular Biology 2023Quote: ... together with dNTP mix containing 10 mM of each nucleotide (Promega). Combined reaction mixtures were incubated at 50°C for 10 minutes prior to inactivation at 85°C for 10 minutes.
-
bioRxiv - Molecular Biology 2023Quote: ... in the presence of 1 mM ATP and 1 unit/uL RNasin® (Promega, N2515) for 30 minutes at 37°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Linear plasmids were transcribed in vitro using the T7 RiboMAX Express Large Scale RNA Production System (Promega) using ‘half sized’ reactions whereby 250 ng linear plasmid was added to a reaction mixture using half the volume suggested for all reagents in the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1.5 µL 10 mM dNTPs (Promega, #C1141), 10 µL 5X LongAmp Taq Reaction Buffer in 50 µL ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1.5 µL 10 mM dNTPs (Promega, #C1141), 10 µL 5X LongAmp Taq Reaction Buffer in 50 µL ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1.5 µL 10 mM dNTPs (Promega, #C1141), 10 µL 5X LongAmp Taq Reaction Buffer in 50 µL ...
-
bioRxiv - Molecular Biology 2023Quote: Protein samples were treated with Endoprotease Lys-C (Fujifilm Wako chemicals, Osaka, Japan) and Trypsin (Trypsin Gold Mass Spec Grade, Promega). Peptide samples were desalted using OMIX C18 pipette tips (Agilent Technologies) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Luciferase activity was measured with the Luciferase Reporter Assay Kit (Promega).
-
bioRxiv - Molecular Biology 2023Quote: ... HAP1 and HAP1-dEGFPY66S cells were imaged in imaging medium (FluoroBrite™ DMEM, 10% FBS, 2.5 mM CaCl2) supplemented with 2 µL CellTox™ Green (Promega, G8741) and 5 µL Annexin V-AV647 (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed using GoTaq qPCR Master Mix (Promega). Reaction mixes were made up in 10 μL total volume containing 500 nM forward and reverse primers (Table 1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Luciferase activities were measured by Dual-Glo luciferase assays (Promega) and normalized to BSA.
-
bioRxiv - Molecular Biology 2023Quote: In vitro translation was performed using a rabbit reticulocyte lysate system (Promega, L4960). 1.5 μL of purified eIF2A protein or BSA was added to 10 μL rabbit reticulocyte lysate reaction mix ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 µL of 100 ng/µL random hexamer primers (Promega: C1181) was added to each sample ...
-
bioRxiv - Molecular Biology 2023Quote: ... or the control vector pHTN HaloTag CMV-neo (G7721, Promega) and 0.01 or 0.02 µg NanoLuc-tagged SOCS1 or VHL expression constructs ...
-
bioRxiv - Microbiology 2023Quote: The genes were cloned one by one into in pGEM-T Easy Vector (Promega, Madison, USA) and finally in pMG36e vector (Addgene ...
-
bioRxiv - Microbiology 2023Quote: ... The amplified products were stained with a mix of 2X Blue/Orange Loading Dye (Promega, Madison, USA), loading buffer (1X ...
-
bioRxiv - Microbiology 2023Quote: ... lactis strains were extracted using a “Wizard Genomic DNA Purification” kit (Promega, Madison, USA). Real-time PCR was done using a Rotor-Gene Q machine (Qiagen ...
-
bioRxiv - Microbiology 2023Quote: ... The pGEM-T Easy Vector (Promega, Madison, USA) and pMG36e vector (Addgene ...
-
bioRxiv - Microbiology 2023Quote: ... Secondary antibodies used in Western blotting were conjugated to alkaline phosphatase (Promega) or horseradish peroxidase.
-
bioRxiv - Molecular Biology 2023Quote: ... 1.5μg of plasmid was transfected into 6-well dishes using Fugene HD (Promega, Madison, WI, USA) with 3:1 transfection reagent to DNA for 24 hours ...
-
bioRxiv - Microbiology 2023Quote: ... 8μl of Trypsin/LysC solution (100ng/μl 1:1 Trypsin (Promega) and LysC (FujiFilm ...
-
bioRxiv - Microbiology 2023Quote: ... 100 μl CellTiter-Glo Reagent (CellTiter-Glo Luminescent Cell Viability Assay, Promega, cat. G7571) was added to each well for 10 min before luminescence was measured with PerkinElmer EnSight microplate reader ...