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Citations for Promega :
5201 - 5250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The reaction was done using GoTaq® Flexi (Promega, Cat # M829) on a GeneAmp® PCR System 2700 (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... An additional 0.1 μg of trypsin (Promega) was added to the samples and digested over night at 25°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were harvested and the medium was exchanged to CO2 independent medium (Life Sciences) containing Endurazine substrate (PROMEGA) according to the manufacturers protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... NanoBRET NanoGlo Substrate + Extracellular NanoLuc Inhibitor (Promega, N2540) was added as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... using FuGENE 4K (PROMEGA) following the manufacturer’s protocol with the LgBIT ...
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bioRxiv - Molecular Biology 2023Quote: ... NanoBRET NanoGlo Substrate + Extracellular NanoLuc Inhibitor (Promega, N2540) was added as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: The HDAC-Glo I/IITM screening system (Promega, cat#G6430)69 was adapted to measure the effect of compounds on the enzymatic activity of human and yeast HDACs (see Note S2) ...
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase activity was measured 48 h after transfection using the Luciferase Assay System kit (Promega) on a Enspire luminometer (Perkin-Elmer) ...
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bioRxiv - Molecular Biology 2023Quote: ... and samples were subsequently digested with trypsin enzyme (Promega) at 25 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... and digested with trypsin enzyme (Promega) at 25 °C ...
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bioRxiv - Molecular Biology 2023Quote: HeLa cells were transfected using Fugene HD reagent (Promega, E2311) at a 3:1 Transfection Reagent:DNA ratio ...
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bioRxiv - Molecular Biology 2023Quote: NanoLuc Binary Technology (NanoBiT, Promega) is originally a structurally complementary reporter system to evaluate protein-protein interaction in cells 36 ...
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was incubated at 25°C for 30 minutes and the production of ADP was measured using the ADP-Glo kinase assay kit (Promega). Vmax was calculated after fitting the kinase activity data to the Michaelis–Menten equation ...
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bioRxiv - Neuroscience 2023Quote: ... The Atp6v1h promoters were cloned into pGL3 plasmid (Promega). The CLEAR mutant promoters were generated using site-directed mutagenesis (QuikChange II XL Site-Directed Mutagenesis Kit ...
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bioRxiv - Neuroscience 2023Quote: ... Thereafter cell viability was measured using CellTiter-Blue Assay (Promega) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: ... Immediately after the addition of the luciferase substrate (Promega) at a 1:1 ratio ...
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bioRxiv - Microbiology 2023Quote: MTT (CellTiter 96 nonradioactive cell proliferation assay, Promega) and XTT (cell viability and proliferation assay ...
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bioRxiv - Microbiology 2023Quote: ... and Nluc activity was determined by adding Nano-Glo luciferase substrate (Promega) and quantified in a microplate reader (rVACV Nluc/Scarlet or rVACV Nluc/GFP) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was collected from these isolates using the Wizard Genomic DNA Purification kit (Promega) and submitted to the McGill Genome Center for library preparation and sequencing using a 600 cycle MiSeq v3 reagent kit.
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bioRxiv - Microbiology 2023Quote: ... The copy numbers of the control DNA/RNA were calculated based on the molecular weight and absorbance at 260 nm of RNA that had been serially diluted (≥3 steps) and/or fluorescence using the Quantus Fluorometer (Promega, Madison, WI, USA) (n = 3).
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bioRxiv - Microbiology 2023Quote: ... and TdT enzyme in TdT buffer (ProMega). This DNA was then subject to an initial round of PCR amplification with primers oTB535 and oTB536 with the Easy A cloning kit (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... The lysis buffer contained 1:4000 NanoGlo (furimazine) substrate (Promega, USA), to generate a bioluminescence signal through substrate hydrolysis 59 ...
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bioRxiv - Microbiology 2023Quote: The assay was adapted from 15 where Hyp1-Nluc schizonts at 1% hematocrit and 1-2% parasitaemia were lysed in 1x NanoGlo buffer (Promega, USA) within a 96-well flat-bottom plate and parasite lysates were added to compounds and incubated for 10 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: Cell growth was monitored using Cell Titer 96 AQueous One proliferation assay (MTS) (Promega, Madison, WI) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Each fly was then lysed in 50 µl Passive Lysis Buffer (Promega) and homogenized with approximately 10 steel beads in a ‘Bullet Blender Blue’ Homogenizer at speed 10 for 2 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lines were routinely tested for mycoplasma contamination using the MycoAlert® kit (Promega, Madison, WI).
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bioRxiv - Immunology 2023Quote: ... samples were diluted 1:1 with water and digested for 1.5 hours at 37 °C with 1 µg of LysC and overnight at 37 °C with 1 µg trypsin (Promega). The peptide mixture was acidified with trifluoroacetic acid (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... The templates were then used to produce dsRNA using the T7 RiboMAX Express Large Scale RNA Production System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 58 °C for 45 seconds and 72 °C for 1 minute (32 cycles) and 72 °C for 10 minutes (1 cycle) using GoTaq G2 DNA polymerase (Promega). Additionally ...
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bioRxiv - Cell Biology 2023Quote: pGL2-Basic (Promega, E1641) or pGLCD36 (Shore ...
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bioRxiv - Microbiology 2023Quote: Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) system for RT-PCR (Promega) was used for cDNA synthesis using 500 ng extracted RNA from all samples ...
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bioRxiv - Microbiology 2023Quote: ... Immunoreactive bands were detected with alkaline phosphatase conjugated secondary antibodies and NBT/BCIP (Promega). Blots shown correspond to representative results of at least 2 independent experiments ...
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bioRxiv - Microbiology 2023Quote: ... and plasmid extractions were done using the Wizard Plus SV Minipreps DNA Purification System according to the manufacturer’s protocol (Promega). The pk18mobsacB vector contains a kanamycin resistance cassette and the sacB gene ...
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bioRxiv - Microbiology 2023Quote: ... and 2.5 U GoTaq DNA polymerase (GoTaq Green Flexi Kit, Promega) was added to 10 μL genomic DNA template mixture yielding 50 μL total reaction volume ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 10 uL of 1X Green GoTaq Flexi Buffer (Promega, Madison, WI, USA) and stored at −80°C ...
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bioRxiv - Immunology 2023Quote: ... was inserted upstream of the luc2 gene in the pGL4.10[luc2] plasmid (Promega Cat. E6651). As the SNP was located within the promoter region ...
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bioRxiv - Immunology 2023Quote: ... was inserted upstream of the luc2 gene in the pGL4.10[luc2] plasmid (Promega Cat. E6651). Following this step ...
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bioRxiv - Cancer Biology 2023Quote: ... All PCR reactions were run using 2 µL of extracted DNA in GoTaq® PCR Master Mix (Promega) and the following cycling protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... and luciferase readings were acquired using the automated dual injector GloMax Luminometer (Promega). Relative WβS levels were estimated by normalizing
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bioRxiv - Cancer Biology 2023Quote: ... Apoptosis was measured by detecting Caspase 3/7 activity after 24 hours of treatment using the Caspase-Glo 3/7 Assay System (Promega) on a BMG CLARIOstar plate reader ...
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bioRxiv - Neuroscience 2023Quote: ... levels of lactate dehydrogenase (LDH) in the culture medium were quantified using CytoTox 96® cytotoxicity assay (Promega, G1780) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... and quantity measured by a Quantus fluorometer (Promega). HiFi libraries were then prepared according to the manual “Procedure & Checklist - Preparing HiFi SMRTbellR Libraries using SMRTbell Express Template Prep Kit 2.0” with an initial DNA fragmentation by g-Tubes (Covaris ...
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bioRxiv - Plant Biology 2023Quote: ... Quality was assessed by Agilent Bioanalyser and the amount was calculated by an RNA-specific kit for Quantus (Promega). An Illumina-compatible library was prepared with the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina ® and finally sequenced on a HiSeq 3000 at the Max Planck-Genome-centre Cologne.
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bioRxiv - Microbiology 2023Quote: ... then treated with RNAse A (ProMega) and Proteinase K (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... and the addition of 1:1000 Nano-Glo (Promega). The bioluminescent signal was measured using a CLARIOstar luminometer (BMG Labtech ...
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bioRxiv - Microbiology 2023Quote: ... After the addition of 1x Nano-Glo Lysis Buffer and 1:1000 Nano-Glo substrate (Promega, USA), relative light units (RLU ...
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bioRxiv - Microbiology 2023Quote: ... Each PCR product was digested with XhoI and BamHI and ligated with the T4 DNA ligase (Promega, Cat #: M1794) into a XhoI and BamHI pre-digested pcDNA3.1-mCherry-HIS vector ...
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bioRxiv - Microbiology 2023Quote: ... Nanoluciferase bioluminescence was measured from the Hyp1-Nluc parasites by lysing the parasite culture 1:5 with 1x Nano-Glo Lysis Buffer (Promega, USA), and the addition of 1:1000 Nano-Glo (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... jejuni NCTC11168 WT (CSS-5295) gDNA using oligonucleotides CSO-4744 x 4745 and ligated into pGEM-T Easy (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were further digested by adding 0.5 µg sequencing grade modified trypsin (Promega) at 25°C ...