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Citations for Promega :
5301 - 5350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: DNA of selected phage-resistant mutants was isolated using a Wizard Genomic DNA purification kit (Promega Corp.) according to the guidelines provided by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... Cell supernatants were assayed for glutamine consumption (Promega #J8021) and Lactate production (Promega #J5921 ...
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bioRxiv - Microbiology 2023Quote: ... DNA was quantified using the Quantus system (Promega Corp.). The integrity of samples was analyzed with TapeStation 2200 (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then cleaned up using the ProNex Size-Selective Purification System (Promega, Wisconsin, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and Lactate production (Promega #J5921) according to the kit specifications ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were digested with MS Grade trypsin (Promega, Madison, WI, USA) on 10 kDa Microcons (Merck-Milipore ...
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bioRxiv - Microbiology 2023Quote: ... A 1 kb DNA ladder (Promega) was used as a size marker ...
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bioRxiv - Microbiology 2023Quote: ... All diagnostic PCR analyses were performed using GoTaq Green (Promega) under the following conditions ...
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bioRxiv - Microbiology 2023Quote: ... Halo TEV protease (Promega, USA) cleavage was performed in 1 ml buffer at 4 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... and the CellTiter-Glo® luminescent reagent (Promega) was used to quantify cell viability as described previously [8] ...
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bioRxiv - Microbiology 2023Quote: ... In Ghent the optical density was measured by diluting the culture in phosphate-buffered saline in a 96-well microtiter plate and measuring the turbidity in a plate reader at 600 nm (GloMax® Explorer Multimode Microplate Reader, Promega).
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bioRxiv - Microbiology 2023Quote: ... using the CellTiter-Glo® luminescent reagent (Promega) as previously described [8].
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bioRxiv - Microbiology 2023Quote: ... Trypsin/LysC (Promega) was added twice at 1:100 (wt:wt ...
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bioRxiv - Microbiology 2023Quote: Viability was assessed using the CellTiter-Fluor Cell Viability Assay (Promega, G6080) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Cells were cultured in the presence of drug for 72 hours prior to addition of CellTiter-Glo 2.0 reagent (Promega) and collection of luminescence values in relative luminescence units (RLU ...
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bioRxiv - Microbiology 2023Quote: ... The gel lanes corresponding to pulsed-SILAC biological triplicates were cut alike into five bands and subjected to a manual in-gel digestion with modified porcine trypsin (Trypsin Gold, Promega). After destaining ...
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bioRxiv - Microbiology 2023Quote: ... Mouse anti-HiBiT tag mAb (30E5, Promega) and goat anti-mouse IgG H&L conjugated with Alexa Fluor 568 (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... The bmrB riboprobe was transcribed in vitro using T7 RNA polymerase (Promega) and labeled with [α-32P]-UTP using a PCR fragment amplified with oligo pair CC2195/CC2196 as template ...
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bioRxiv - Microbiology 2023Quote: ... cells were lysed with 25 μl of the Nano-Glo HiBiT lytic detection system (Promega, Madison, USA) plus 25 μl of PBS ...
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bioRxiv - Microbiology 2023Quote: ... The value of relative light unit of luciferase was determined using the PicaGene Luminescence Kit (TOYO B-Net Co., Ltd., Tokyo, Japan) and GloMax Navigator System G2000 (Promega Corporation, Madison, WI, USA), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... The 3809-bp PCR product was purified and cloned into pGEM-T-Easy (Promega, Medison WI, USA) to yield pGEM ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Microbiology 2023Quote: ... using M-MLV-RT and random hexamer primers (Promega) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... pCI Luc contains firefly luciferase cDNA cloned MluI / Xba I in pCI (Promega). pNLENG1-ES-IRES (a gift of D.N ...
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bioRxiv - Microbiology 2023Quote: ... The standard curve was prepared using Bovine Serum Albumin (Promega). Absorbance was measured at 595 nm with a spectrophotometer.
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bioRxiv - Molecular Biology 2023Quote: ... were mixed with 75ul of 0.1% Triton X-100 in PBS and 25 μl of substrate (Bright-Glow™ Luciferase Assay System, Promega), and 50ul aliquots of the mix were distributed in triplicate wells of a 96-wells white plate and analyzed in a microplate luminometer.
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bioRxiv - Molecular Biology 2023Quote: RNA samples were subjected to DNase digestion using RQ1 RNase-free DNase (Promega) according to the manufacturer’s instructions ...
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Induction of osteogenic differentiation of MSCs by GSK3β knockdown through GSK3β siRNAs transfectionbioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was performed using the ImProm-II™ Reverse Transcription System reagent kit (Promega, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... and yeast-optimized nanoluciferase (Promega Cat. No. N1141) were amplified using PCR and cloned into the XhoI and HindIII-digested single-copy yeast integrating plasmid using Gibson assembly.
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bioRxiv - Molecular Biology 2023Quote: ... FuGENE HD (#E2312, Promega, Walldorf, Germany), and linear polyethylenimine (PEI ...
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bioRxiv - Microbiology 2023Quote: Nanoluciferase (Nluc) gene was obtained from the pNL1.1 plasmid (Promega Corporation, USA). The nanoluciferase encoding gene was fused to the C-terminus of either CFP-10 or Rv1636 following a short linker sequence (GGSGGGSSGG ...
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bioRxiv - Molecular Biology 2023Quote: ... Each of the enzymes [trypsin (Promega, Madison, WI, USA), GluC (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... or stimulated for 10 minutes or 1 hour by BRET through activation of NanoLuc’s bioluminescence with its substrate furimazine (1:100 dilution of Promega nano-Glo Live Assay). After treatment ...
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bioRxiv - Molecular Biology 2023Quote: The Nano-Glo® luciferase assay system (Promega #N1120) was used to assess NLuc expression by SARS-CoV-2 NLucFL ...
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bioRxiv - Molecular Biology 2023Quote: ... and NanoLuc luciferase reporter gene expression was detected using a Nano-Glo® Luciferase Assay System (Promega) and analyzed on a Infinite M200 plate-reader (Tecan).
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated using the WIZARD Genomic DNA Purification kit (Promega, Madison, WI, USA), and PCR amplicon libraries of the V1-V2 region of the 16S rRNA gene were constructed using uniquely barcoded primers with primers V1_A_Pyro_27F (5’ s-CGTATCGCCTCCCTCGCGCCATCAGTCAGAGTTTGATCCTGGCTCAG-3’ ...
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bioRxiv - Microbiology 2023Quote: ... 10 μL 5X Green GoTaqR Reaction Buffer (Promega), 1 μL dNTPs mix (each deoxynucleotide at 10 mM) ...
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bioRxiv - Microbiology 2023Quote: An RNA probe was synthesized in vitro from pSPT19 AS1-S using T7 RiboMAX Large Scale RNA Production Systems (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting templates were purified using the Wizard® SV Gel and PCR Clean-Up System kits (Promega) and transcribed using the Megascript T7 kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μl cDNA of WT and CRISPR cln3 mutant lines was used as PCR template and amplicons were cloned into the pGEMT vector following the manufacturer’s instructions (Promega). Selected clones were sent for sequencing.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were digested overnight in 25 mM ammonium bicarbonate with trypsin (Promega) for mass spectrometric analysis ...
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bioRxiv - Molecular Biology 2023Quote: ... this reaction was spiked at 1% into a GoTaq HotStart (Promega) PCR reaction with primers P3HDV and P5XHPγn ...
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bioRxiv - Molecular Biology 2023Quote: ... a mixture of the 3 Cas9-sgRNA plasmids (150ng/μl) was transfected into HEK293 cells at 70% confluency in fresh medium using FuGENE® HD Transfection Reagent (Promega). Two days after transfection ...
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bioRxiv - Molecular Biology 2023Quote: Predigestion of bound proteins was performed by addition of 200 ng / µl of a 1:1 LysC (FUJIFILM Wako Pure Chemical Corporation, 125-02543) trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) mix ...
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bioRxiv - Molecular Biology 2023Quote: ... at an enzyme-to-substrate ratio of 1:100 and subsequently digested overnight at 37°C with sequencing grade trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Molecular Biology 2023Quote: ... Luminescence was measured in a Promega™ GloMax® 20/20 Luminometer (Promega #E5311) with an integration time of 10 seconds.
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bioRxiv - Molecular Biology 2023Quote: ... 0.3 unit of Taq DNA polymerase (Promega, Shanghai, China) and ~40 ng of genomic DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... and finally digested with 17 ng/μl sequencing-grade trypsin (Promega) in 25 mM ABC ...
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bioRxiv - Microbiology 2023Quote: ... 0.1 μL of DNA polymerase (Promega, Madison, USA) at 50 ng/μL and enough water in a final volume of 12.5 μL ...
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bioRxiv - Microbiology 2023Quote: ... and 1:10000) were established using the genes previously cloned in pGEM-T Easy Vector (Promega, Madison, USA). The data were determined in terms of copies of the nisA and nisRK genes / μL of qPCR reaction ...