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Citations for Roche :
2801 - 2850 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... and PCR enriched (8-10 cycles) using the KAPA Hyper Library Preparation kit (KAPA Biosystems, #KK8504). eCLIP libraries were prepared as described above according to (Van Nostrand et al. ...
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bioRxiv - Microbiology 2022Quote: Quantitative PCR (qPCR) was performed using LightCycler 480 SYBR Green I Master mix (ref.04707516001- Roche) or IQ Supermix (Ref ...
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bioRxiv - Immunology 2022Quote: ... Quantitative PCR (qPCR) was performed with a LightCycler 480 II real time system (Roche, Basel, Switzerland) using FastStart Essential DNA Probes Master (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... Polymerase chain reactions (PCR) on bisulfite converted DNA was performed with FastStart Taq DNA Polymerase (Roche) in the following conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... qRT-PCR experiments with the resulting cDNA samples were carried out on a Lightycler 480 (Roche) using qPCRBIO SyGreen Blue Mix (PCRBiosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... The following PCR conditions were used: 5µL KAPA HiFi Hotstart Fidelity Buffer (5X) (KAPA Biosystems, USA), 1.25µL Forward Primer (10µM) ...
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bioRxiv - Genomics 2020Quote: ... The resulting double-stranded cDNAs were amplified by PCR using KAPA HiFi DNA Polymerase (Kapa Biosystems), Ligation Sequencing Kit Primer Mix (provided by the ONT 1D Kit) ...
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bioRxiv - Genomics 2019Quote: ... mRNA expression was analyzed by quantitative PCR (qPCR) with SYBR Green using a LightCycler 480 (Roche). All qPCR primer sequences used in this study are listed in Table S1.
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bioRxiv - Immunology 2021Quote: ... Reverse: 3’-GGTCCACCAAACGTAATGCG-5’) were performed using KAPA SYBR FAST ONE-STEP qRT-PCR kits (Roche) according to manufacturer’s instructions on a Lightcycler 480 Instrument-II (Roche).
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bioRxiv - Developmental Biology 2020Quote: ... Amplification reactions were performed in a LightCycler 480 Real-Time PCR System (05015243001, Roche, Basel, Switzerland) in 384 well plates ...
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bioRxiv - Microbiology 2019Quote: ... Fragments MD1 (8530bp) and MD3 (1079bp) were amplified by Expand Long Template PCR System (Roche, Germany) from MDMV genome using M1F/M1R and M3F/M3R ...
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bioRxiv - Developmental Biology 2019Quote: ... Quantitative real-time PCR was conducted with LightCycler-FastStart DNA Master SYBR Green I kit (Roche) using a LightCycler 480 Real-Time PCR System (Roche) ...
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bioRxiv - Plant Biology 2019Quote: The satellite-specific probes were labelled by PCR in the presence of biotin-16-dUTP (Roche). We used nick translation to mark the probes for the ribosomal genes ...
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bioRxiv - Molecular Biology 2019Quote: ... Real-time PCR was performed using the FastStart Universal SYBR Green Master [Rox] (Hoffmann-La Roche) with miRNA forward primer and universal primer (URP) ...
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bioRxiv - Immunology 2019Quote: ... HDR donor vectors were linearized by PCR with the KAPA Hifi HotStart ReadyMix (KAPA Biosystems, KK2602) using either p9 and p10 or p11 and p12 (HA 750bp each ...
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bioRxiv - Cancer Biology 2019Quote: ... for cDNA amplification and the qRT-PCR was carried out in a LightCycler 480 II (Roche) for signal detection ...
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bioRxiv - Bioengineering 2019Quote: ... and their DNA was amplified by PCR using 2x KAPA HiFi HotStart ReadyMix (KAPA Biosystems, MA) with 2.5 μL of phage DNA template and 10 μL of 1μM primers (NGS1F and NGS1R ...
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bioRxiv - Genomics 2019Quote: ... The size-selected library pool was quantified by quantitative PCR (qPCR; Kapa Biosystems, Wilmington, MA, USA) and adjusted to 2 nmol/L ...
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bioRxiv - Biochemistry 2021Quote: The RBD domain variants (see Table S1) were PCR amplified (KAPA HiFi HotStart ReadyMix, Roche, Switzerland) from codon-optimized SARS-CoV-2 Spike protein gene (Sino Biological ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR amplifications were performed on a LightCycler 480 thermal cycler (Roche Diagnostic, Mannheim, Germany) in a final volume of 10 μl ...
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bioRxiv - Cell Biology 2020Quote: ... Single-stranded DNA fragments were linearly amplified via 14 PCR cycles (HiFi HotStart ReadyMix, Kapa Biosystems).
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bioRxiv - Microbiology 2021Quote: ... Labelled DNA was generated using the same PCR program with the DIG Easy Hyb (Roche, 1603558) mix ...
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bioRxiv - Bioengineering 2021Quote: ... Quantitative Real-Time polymerase chain reaction (qRT-PCR; Light-Cycler 480 instrument Roche Diagnostics, Mannheim, Germany) analysis was performed for Collagen Type I (COL1A1) ...
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bioRxiv - Bioengineering 2021Quote: ... The Real-time quantification PCR data were analyzed in LightCycler® 96 Application (Roche Applied Science) and exported into an Excel datasheet for further analysis ...
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bioRxiv - Bioengineering 2020Quote: ... and the qRT-PCR analysis was performed using LightCycler 480SYBER Green I Master Mix (Roche Diagnostics), (Supplementary Table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... 8 μL of each sample E3 was used directly for PCR as described above (KAPA Biosystems). Embryos were kept in 48-well plates until genotyping was achieved.
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bioRxiv - Cancer Biology 2020Quote: ... PCR was performed using a master mix including the Expand high fidelity Taq polymerase (Roche Diagnostics). Amplicons were analyzed by standard agarose gel-electrophoresis ...
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bioRxiv - Bioengineering 2021Quote: Second-round library PCR (Fig. 1H) was performed using Kapa HiFi Hotstart Readymix (KK2602, KAPA Biosystems) in 100 μl reaction volume with 10 μl of 2 nM first-round PCR product as a template and forward (AAT GAT ACG GCG ACC ACC GAG ATC TAC ACT CTT TCC CTA CAC GAC GCT CT*T *C ...
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bioRxiv - Plant Biology 2021Quote: ... All qPCR reactions were performed in a LightCycler® 96 Real-Time PCR System (Roche Diagnostics) using the FastStart Essential DNA Green Master (Roche Diagnostics) ...
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bioRxiv - Microbiology 2020Quote: ... Relative quantification of gene expression was performed using a LightCycler 480 Real-Time PCR System (Roche). Forward and reverse primers were combined with SYBR Green PCR Supermix (Bio-rad ...
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bioRxiv - Microbiology 2020Quote: ... except the Enhanced PCR Mix (EPM) was substituted with KAPA HiFi HotStart Uracil+ ReadyMix (Kapa Biosystems) to enable the amplification of uracil encountered in the DNA template ...
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bioRxiv - Genomics 2021Quote: ... Library amplification (13 PCR cycles) was performed with KAPA HiFi HotStart Uracil+ DNA polymerase (Kapa Biosystems). Library size was determined by the Agilent 4200 Tapestation system ...
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bioRxiv - Neuroscience 2020Quote: ... All the PCR reactions were carried out with Kapa HiFi 2 x mastermix (KK2601, Kapa Biosystems). PCR products were then separated by 2% agarose gel ...
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bioRxiv - Microbiology 2020Quote: ... Amplicon PCRs were conducted in 25 μL reactions each containing 1 X Buffer (KAPA Biosystems, USA), 1.5 mM MgCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative PCR reactions were carried out using FastStart Universal SYBER Green Master mix (Roche Applied Science) on a StepOnePlus system (Applied Biosystems) ...
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bioRxiv - Biophysics 2020Quote: ... and a biotin molecule (Supplementary Note 1) via PCR amplification with KAPA Hi Fi Polymerase (Roche) and nicking near the ends by the site-specific nicking enzyme Nt.BspQ1 (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... quantitative real-time PCR was performed with Light Cycler 480 SYBR Green I Master (Roche, Switzerland) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR reactions were performed in LightCycler 480 96-well plates (Roche Diagnostics, Indianapolis, IN, USA) using a 10 minute pre-incubation period at 95°C followed by 40 cycles of denaturation for 5 seconds at 95°C ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was extracted from 106 cells using High Pure PCR Template Preparation Kit (Roche, 11796828001). 150 ng of purified DNA was digested in Reaction Buffer (200 mM sodium acetate [pH 4.5] ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR reactions were performed using the LC480 Instrument with Sybr Green PCR master mix (Roche, 04707516001) according to manufacturer’s protocol and using primers indicated in Supplementary Table 2 ...
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bioRxiv - Genomics 2022Quote: ... PCR amplifications for Nanopore library preparations were made with Kapa Hifi Hotstart polymerase (Roche Sequencing Solutions): initial denaturation ...
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bioRxiv - Molecular Biology 2022Quote: ... All PCR reactions were carried out with the LightCycler 480 SYBR Green I Master (Roche, 04887352001) according to the manufacturer’s protocol and amplification was quantified on an LightCycler 480 II Real-Time PCR System (Roche) ...
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bioRxiv - Cancer Biology 2022Quote: ... gRNA DNA sequences were PCR amplified (empirically determined number of cycles; KAPA HiFi HotStart ReadyMix; Roche), SPRI purified (AMPure XP SPRI beads ...
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bioRxiv - Cancer Biology 2020Quote: ... The quantification and quality of final libraries were determined using KAPA PCR (Kapa Biosystems, Waltham, MA) and a high-sensitivity DNA chip (Agilent Technologies) ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR was performed on a LightCycler480 Real-Time PCR detection system (Roche Applied Science, Penzberg, Germany) with standard PCR parameters ...
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bioRxiv - Cell Biology 2021Quote: ... PCR array were performed in 384-well plates on a LightCycler 480 instrument (Roche Applied Science). The reaction mix of 102 ul of sample cDNA was prepared using 2x SA Biosciences RT2 qPCR Master Mix and 10 ul of this mixture was added into each well of the PCR array ...
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bioRxiv - Cell Biology 2021Quote: ... quantitative real-time PCR was performed with Light Cycler 480 SYBR Green I Master (Roche, Switzerland) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... PCRs were prepared containing 1x KAPA Taq Buffer A (includes 1.5 mM MgCl2) (Kapa Biosystems, USA), dNTP nucleotide mix (0.2 mM each) ...
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bioRxiv - Microbiology 2022Quote: ... Real-time PCR amplifications were performed on a LightCycler 480 thermal cycler (Roche Diagnostic, Mannheim, Germany) in a final volume of 10 µl ...
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bioRxiv - Genomics 2022Quote: ... mRNA expression was analyzed by quantitative PCR (qPCR) with SYBR Green using a LightCycler 480 (Roche).