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Citations for Roche :
251 - 300 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Cells were then washed with ice-cold PBS two times and lysed in 300 μl RIPA buffer (50 mM TRIS pH 8, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, Roche Complete protease inhibitors ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then washed with ice-cold PBS two times and lysed in 300 μl RIPA buffer (50 mM TRIS pH 8, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, Roche Complete protease inhibitors ...
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bioRxiv - Bioengineering 2024Quote: ... in a Roche LightCycler 96 system (Roche Diagnostics, Penzberg, Germany). Actin was used as a reference sequence to calculate the 2−ΔΔCT value (Gao et al. ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1% sodium dodecyl sulfate (SDS) and 1% TritonX-100) supplemented with Protease Inhibitor (Roche, 11873580001). Extracted proteins were boiled for 10 min with 2X Laemmeli buffer (100mM Tris-HCl pH6.8 ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM β-mercaptoethanol and 20 μM GDP supplemented with DNase I (10 μg/ml) and cOmplete protease inhibitor cocktail tablets (Roche). Cells were mechanically disrupted using a Microfluidizer (Microfluidics International Corporation ...
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bioRxiv - Bioengineering 2024Quote: ... Shotgun libraries were constructed using the Kapa Hyper Prep Sample Preparation Kit (Roche, Indianapolis, IN). For each sample ...
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bioRxiv - Bioengineering 2024Quote: ... The adaptor-ligated DNA was amplified by PCR for 8 cycles with the Kapa HiFi polymerase (Roche, Indianapolis, IN). The final libraries were quantitated on Qubit and the average size determined on the AATI Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was loaded on a column filled with cOmplete His-tag Purification Resin (Roche Diagnostics GmbH, Mannheim, Germany) and pre-equilibrated with lysis buffer ...
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bioRxiv - Immunology 2024Quote: ... utilizing the X-tremeGENE HP DNA Transfection Reagent from Roche (Basel, Switzerland). After 48 hours ...
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bioRxiv - Microbiology 2024Quote: ... Detection of mRNA was performed with anti-DIG-alkaline-phosphatase-conjugated antibody (Roche Diagnostics) and the alkaline phosphatase substrate 5-bromo-4-chloro-3-indolyl phosphate (BCIP ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 mM β-mercaptoethanol and protease inhibitors from Roche according to the manufacturer’s instructions) ...
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bioRxiv - Biochemistry 2024Quote: ... 9 μl of qPCR reaction was set up in 384 well plates with 4.5 μl of 2X FastStart Universal SYBR Green Master mix ROX (Roche), ∼0.28 μM of primers (Set 1 ...
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bioRxiv - Biochemistry 2024Quote: ... pHM6 (Roche Applied Science), which carries a cDNA encoding the recombinant human ETB receptor ...
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bioRxiv - Biochemistry 2024Quote: ... cOmplete™ EDTA-free Protease Inhibitor (ROCHE), at 1 g wet cells per 5 mL buffer ...
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bioRxiv - Biochemistry 2024Quote: ... harvested cells were resuspended with ice-cold lysis buffer (50 mM Tris-Cl pH 8.0, 300 mM NaCl, and complete, EDTA-free protease inhibitor cocktail tablets (Roche)) at a ratio of 3.1 mL/g of cell pellet ...
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bioRxiv - Biochemistry 2024Quote: ... cells were lysed in RIPA buffer supplemented with protease inhibitors (Roche) and protein was quantified by Pierce® Bicinchoninic acid (BCA ...
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bioRxiv - Biochemistry 2024Quote: ... tissues were lysed in RIPA buffer (ratio 1:4) supplemented with protease inhibitors (Roche) and protein was quantified by BCA Protein Assay Kit assay.
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bioRxiv - Biochemistry 2024Quote: ... cells were lysed in RIPA buffer supplemented with protease inhibitors cocktail (Roche). Lysates were centrifuged at 20000 × g at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... 20,000g for 20 min and the pellet was resuspended in 50 µl of PBS supplemented with 1 mM TCEP and cOmplete protease inhibitor (Roche, 11836170001). Membrane proteins were solubilized and PEGylated by adding 7 µl of 10% β-d-dodecyl maltoside (Anatrace 329370010 ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 mM PMSF) and supplemented with a protease inhibitor cocktail (cOmplete Protease Inhibitor Cocktail Tablets, EASYpack, Roche). Cells were lysed by sonication on ice (Branson Digital Sonifier 450 ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with protease inhibitor cocktail (Roche) and 5 mM Sodium Fluoride and 5 mM Sodium Orthovanadate.
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) + protease inhibitors (1 tablet / 50 ml Roche Complete Ultra EDTA-free ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM benzamidine) + protease inhibitors (1 tablet / 50 ml Roche Complete Ultra EDTA-free ...
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bioRxiv - Biochemistry 2024Quote: ... 35 mM imidazole) + protease inhibitors (1 tablet / 50 ml Roche Complete EDTA-free, 1 mM AEBSF), lysozyme ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) + protease inhibitors (1 tablet / 50 ml Roche Complete Ultra EDTA-free ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) + protease inhibitors (1 tablet / 50 ml Roche Complete Ultra EDTA-free ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) + protease inhibitors (1 tablet / 50 ml Roche Complete Ultra EDTA-free ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) + protease inhibitors (1 tablet / 50 ml Roche Complete Ultra EDTA-free ...
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bioRxiv - Biochemistry 2024Quote: ... 50mM TrisHCL (pH 7.5) supplemented with protease inhibitor tablets (Roche). The homogenate was sonicated 3x for 30 sec and incubated on ice for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT and protease inhibitors (Roche) and sonicated 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... pH 8.5 with the addition of EDTA-free protease inhibitor cocktail (Roche). Cells were then lysed using ultrasonication and centrifuged to clarity ...
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bioRxiv - Biochemistry 2024Quote: ... and protease inhibitor cocktail (Complete-EDTA free ULTRA, Roche) (buffer A) ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were harvested and resuspended in twice the cell pellet volume of lysis buffer (50 mM potassium phosphate pH 8.0, 0.5 M NaCl, 20 mM Imidazole, cOmplete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Biochemistry 2024Quote: ... A ∼3:1 ratio of X-tremeGENE™ 9 DNA Transfection Reagent (Roche, XTG9-RO) was added to the mixture prior to incubation and application on HEK293T cells using standard methods 1 ...
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bioRxiv - Biochemistry 2024Quote: ... EDTA-free Protease Inhibitor Cocktail (Roche) and 250 U/ml benzonase ...
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bioRxiv - Bioengineering 2024Quote: ... the infected lungs were transferred to gentleMACSTM C tubes and homogenized on a gentleMACSTM tissue dissociator (“m_lung_01” program) using HEPES buffer (2 mg/ml collagenase D (Roche) and 80 U/mL DNAse I (Roche) ...
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bioRxiv - Bioengineering 2024Quote: ... Indexing PCR was performed using KAPA HiFi HotStart Ready Mix (Roche), 0.067 ng of PCR template and 0.5 μM of indexed primers in the total reaction volume of 25 μL ...
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bioRxiv - Bioengineering 2024Quote: ... and 80 U/mL DNAse I (Roche)) as a medium ...
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bioRxiv - Bioengineering 2024Quote: ... UK) and 0.3 mg/kg Hypnovel (Midazolam, Roche Products, Welwyn Garden City, UK) was performed ...
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bioRxiv - Bioengineering 2024Quote: ... a 10 mM Tris pH 7.4 (Roche 10812846001), 0.25% Igepal CA-630 (Sigma I8896-50ML),150 mM NaCl (Macron 7581-06 ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with protease inhibitor tablets (Roche). IL-10 was quantified by ELISA (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: cDNA from cells was diluted 1:10 and qPCR was performed in triplicate using LightCycler 480 SYBR Green I Mastermix (Roche). Samples containing primers specific for lmna ...
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bioRxiv - Bioengineering 2024Quote: ... the samples were placed in LightCycler® 96 System (Roche, Canada). The reaction was initiated with an initial denaturation step at 95°C for 10-30 seconds with detection turned off ...
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bioRxiv - Bioengineering 2024Quote: ... Products were amplified by PCR with the Kapa HiFi polymerase (Kapa Biosystems, Wilmington, MA), and libraries were quantified by qPCR (Kapa Biosystems ...
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bioRxiv - Bioengineering 2024Quote: ... and libraries were quantified by qPCR (Kapa Biosystems, Wilmington, MA). Libraries were sequenced with 150 bp paired-end reads on the Illumina NextSeq2000 instrument ...
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bioRxiv - Cancer Biology 2024Quote: ... The following commercial kits from Roche-Ventana Medical System were used ...
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Refining Spatial Proteomics by Mass Spectrometry: An Efficient Workflow Tailored for Archival TissuebioRxiv - Bioengineering 2024Quote: ... The slides were counterstained with Hematoxylin II (Roche) for 8 minutes and Bluening Reagent for 4 minutes.
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Refining Spatial Proteomics by Mass Spectrometry: An Efficient Workflow Tailored for Archival TissuebioRxiv - Bioengineering 2024Quote: ... sections were incubated with EpCAM (Ready-To-Use, 790-4465, Roche) for 60 minutes at room temperature ...
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Refining Spatial Proteomics by Mass Spectrometry: An Efficient Workflow Tailored for Archival TissuebioRxiv - Bioengineering 2024Quote: ... and the secondary antibody Omnimap Anti-Mouse (Roche) was applied ...
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bioRxiv - Cancer Biology 2024Quote: Whole cell extracts were prepared by lysing cells in RIPA buffer (50 mM Tris HCl pH 7.4/150 mM NaCl/0.5% sodium deoxycholate/0.1% sodium dodecyl sulfate/1% NP-40) containing cOmplete protease inhibitor cocktail (Roche), followed by quantification using Bradford assays ...