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Citations for Roche :
51 - 100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... by measuring released LDH (Roche Diagnostics, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... 0.5 M EDTA and Protease Inhibitor (Roche). The cell lysate was collected by centrifuging the homogenate at 20,000g for 10 min ...
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bioRxiv - Plant Biology 2024Quote: ... Real-time quantitative PCR assays were prepared using KAPA SYBR FAST qPCR kit (Kapa Biosystems) and run on an Applied Biosystems QuantStudio 12K QPCR instrument ...
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bioRxiv - Plant Biology 2024Quote: ... AV2 RNA was detected with the digoxigenin (Roche Diagnostics GmbH) labelled in vitro transcribed RNA fragment reverse complementary to the AV2 CP ORF ...
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bioRxiv - Plant Biology 2024Quote: ... qRT-PCR was performed on a 480 LightCycler thermocycler (Roche, Indianapolis, IN, USA) following the manufacturer’s instructions with TB Green Premix Ex Taq II (Takara ...
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bioRxiv - Plant Biology 2024Quote: ... total proteins were extracted from 2-3 aliquots of 3 g frozen ground Arabidopsis seedling tissue with IP buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5% NP-40, 1% Triton-X, EDTA-free protease inhibitor cocktail [Roche]). Lysates were cleared by filtering through miracloth followed by centrifugation (6,000 g ...
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bioRxiv - Plant Biology 2024Quote: ... Probes were synthesized using the DIG RNA Labeling Kit (Roche, Mannheim, Germany) for CDS of the FLC gene cloned into the pGEM®-T Easy vector (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... a LightCycler® 480 (Roche) and the primers described below ...
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bioRxiv - Plant Biology 2024Quote: ... using commercial SYBR green mastermix (Roche). Samples were subjected to 2 min pre-incubation at 95°C followed by 40 amplification cycles of 15 s at 95°C ...
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bioRxiv - Plant Biology 2024Quote: ... the volumes of each reagent used were as follows: FastStart Universal SYBR Green Master (Roche) 5.50 μL ...
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bioRxiv - Plant Biology 2024Quote: ... 100 nM of each forward and reverse primer (Supplementary Table S4) and 125lllng λ-DNA (Roche Diagnostics, Vilvoorde, Belgium) was mixed with 2lllμL of a 12x diluted sample cDNA ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR was performed using gene-specific primers (Supplemental Table S3) using Promega Go Taq® qPCR Master Mix on a Light Cycler 480 II® (Roche) thermocycler ...
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bioRxiv - Neuroscience 2024Quote: ... RNA has been retro-transcribed to cDNA using oligo-dT and Transcriptor reverse transcriptase (Roche) according to manufacturer recommendations.
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bioRxiv - Neuroscience 2024Quote: ... Genomic PCR for ATP13A2 floxed or KO alleles was performed using 100 ng genomic DNA and the Kapa2g Fast HotStart PCR Kit (Roche). PCR primers included a forward primer (5’-CTGCAGCTTCGAGAGGAAAG-3’) ...
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bioRxiv - Neuroscience 2024Quote: ... GFP (A-11122; Thermofisher or 11814460001; Roche or GFP-1010; Aves Labs), GAD67 (MAB5406 ...
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bioRxiv - Neuroscience 2024Quote: Primary antibodies used for Western blotting include GFP (11814460001; Roche), pThr73-Rab10 (ab230261 ...
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bioRxiv - Neuroscience 2024Quote: ... using LightCycler 480 (Roche, Basel, Switzerland). The final titration of the AAV was estimated as relative quantitation according to a calibration curve calculated from the known numbers of copies of AAV plasmids.
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Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosisbioRxiv - Neuroscience 2024Quote: Cell viability assays were performed using a WST-8 kit (Roche Diagnostics) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and DispaseII (Roche, 1mg/ml) and minced before placing on thermocycler at 37C for 1h at 1000rpm ...
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bioRxiv - Neuroscience 2024Quote: ... and halt phosphatase inhibitor cocktail (Roche, Indianapolis, IN, USA). Collected samples were sonicated on ice and centrifuged at 10,000 × g for 10 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were moved for digestion in Collagenase A (Roche, 5mg/ml) and DispaseII (Roche ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... and complete protease inhibitor cocktail (Roche). Resuspended lysates were sonicated for 6 x 45s pulses at 90% duty cycle and pelleted for 75 min ...
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bioRxiv - Pathology 2024Quote: ... Real-time PCR was performed using a LightCycler 96 instrument (Roche Diagnostics GmbH, Mannheim, Germany). FAP mRNA expression level was normalized to that of β-actin.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1 mM EDTA and 1×Complete EDTA free-tablet (Roche Diagnostics, Basel, Switzerland) in distilled water ...
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bioRxiv - Neuroscience 2024Quote: ... and 3 mg/ml dispase II (Roche Diagnostics) in CMF Hank’s solution ...
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bioRxiv - Neuroscience 2024Quote: ... Ganglia were then treated for 20 min at 37°C with 3 mg/ml collagenase (type I; Roche Diagnostics) and 3 mg/ml dispase II (Roche Diagnostics ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... hygromycin B was purchased from Roche Applied Science ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... 2% SDS) containing protease inhibitor cocktail (PIC 100X, Roche) and phosphatase inhibitors (Roche) ...
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bioRxiv - Synthetic Biology 2024Quote: PCR amplicons are generated using Kapa HiFi (Roche) PCR kits following the manufacturer protocol (Supplementary Table 1) ...
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bioRxiv - Neuroscience 2024Quote: ... The pooled library was quantified using KAPA Library Quantification Kit (Kapa Biosystems, Cape Town, South Africa) diluted and denatured as the guideline of Illumina’s sequencing library preparation ...
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bioRxiv - Microbiology 2024Quote: ... and then blocked with 1% bovine serum albumin (BSA; Roche #3853143) in PBS for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Library quality control was performed with an Agilent 2100 Bioanalyzer and quantity determined via the KAPA Library Quantification Kit (KAPA Biosystems). WGBS libraries were then pooled to meet the required 1µg DNA input necessary for targeted enrichment ...
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bioRxiv - Neuroscience 2024Quote: ... mRNA levels were measured by quantitative PCR by using a LightCycler480 (Roche, Rotkreuz, Switzerland) using specific Taqman probes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... harvested lungs were minced and digested with Liberase TM (100 µg/mL; Roche, Indianapolis, IN), collagenase XI (250 µg/ml) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The hybridization result was detected with anti-DIG-AP antibody (1:2000, Roche, 11093274910) and NBT/BCIP (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Probes were synthesized using the in vitro DIG-RNA labeling transcription Kit (Roche, 11175025910) with linearized pGEM-T easy vector containing marcksl1a or foxo1a gene fragment as the templates ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Real-time PCR analysis was performed with the LightCycler 480 SYBR Green I Master (Roche) using the ABI Prism 7500 Sequence Detection System instrument ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bronchoalveolar lavage (BAL) was performed by instilling the lungs with phosphate-buffered saline containing a protease inhibitor cocktail (Roche Cat. No. 11836170001) two times ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with Phosphatase Inhibitors (PhosSTOP, Roche). Beads containing GFP-tagged targets were distributed evenly in kinase reaction tubes and resuspended in 50 μL 1x Kinase Buffer containing 1 mM ATP (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with a Protease Inhibitor Cocktail (cOmplete™, Roche), and clarified by centrifugation at 17,000 x g for 10 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... and membrane washing were conducted using the DIG luminescent Detection kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The lcrF transcripts of interest were detected with a DIG-labeled PCR fragment (DIG-PCR nucleotide mix, Roche) amplified with primer pairs for the individually detected according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The reaction mix used was FastStart Essential DNA Green Master mix (Roche; 06402712001). Real time PCR assay was performed on Quant studio 5 real time PCR machine (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and KAPA Library Quantification kit for Illumina (KAPA Biosystems). Sequencing of the 10x Genomics single-cell RNA-seq libraries was performed on an Illumina NovaSeq 6000 (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... and cOmplete mini EDTA-free protease inhibitor cocktail (Cat No. 11836170001, Roche) and sonicated 2x10 sec at 30 amp at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... and 1 ml of 0.1 M PBS supplemented with protease inhibitors (Roche, Switzerland) was used per 20 mg of tissue ...
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bioRxiv - Neuroscience 2024Quote: ... and proteinase inhibitor (Roche, Cat. 04693124001). Lysates containing 500 µg total protein were incubated at 4°C for 2 - 3 hr with one of the following antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... and streptavidin alkaline phosphatase fast red (Roche Diagnostics).
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bioRxiv - Cancer Biology 2024Quote: ... containing a complete mini-protease inhibitor cocktail (Roche), then equal quantities of protein lysate (3-10 µg ...
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bioRxiv - Cell Biology 2024Quote: ... pH 5.5 and 1 mg/mL pronase (both from Roche Diagnostics, Germany), blocked with 5% bovine serum albumin (Sigma Aldrich ...