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Citations for Roche :
51 - 100 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 6 uL X-tremegene-9 transfection reagent (Roche 06365787001), 0.3 μg pCMV-VSV-G (Addgene Plasmid #8454) ...
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bioRxiv - Cell Biology 2024Quote: ... and 1x cOmplete Mini protease inhibitor cocktail (Roche, Mannheim, Germany)) ...
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bioRxiv - Developmental Biology 2024Quote: ... EDTA free protease inhibitor cocktail (Roche)) at left on ice for 10 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... washed once with PBS and resuspended and cryopreserved in wash buffer 150 (20 mM HEPES pH 7.5, 150 mM NaCl, protease inhibitor cocktail (Roche), 0.5 mM Spermidine ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 phosphatase tablet (PhosSTOP – Roche), 20 μM MG132 ...
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bioRxiv - Cancer Biology 2024Quote: ... Isolated hepatocytes were seeded at a density of 4-500,000 and 1,000,000 cells in rat tail collagen I (5 μg/cm2, Roche) pre-coated 6-well plates and T25 flasks ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA plasmids were transfected using X-tremeGENE HP (Roche Diagnostics) whereas siRNA was delivered using Lipofectamine™ RNAiMAX (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... or incubated in 1X RBC lysis solution (Roche #11814389001) for 10 minutes at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... T7 DNA ligase (Roche) 25ng of backbone vector ...
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bioRxiv - Developmental Biology 2024Quote: ... Nuclei were washed once in 1 mL Wash350 (20 mM HEPES pH 7.5, 350 mM NaCl, 10 mM NaButyrate, 0.025% Digitonin, protease inhibitor cocktail (Roche), 0.5 mM Sper-midine ...
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bioRxiv - Developmental Biology 2024Quote: ... Phosphorylated and annealed oligos were diluted 1:10 and a Golden Gate reaction was setup with 1x rapid ligase (Roche), 10 units of BbsI enzyme ...
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bioRxiv - Cancer Biology 2024Quote: ... with 1mg/ml of DNase (Roche, 10104159001) and 200U of collagenase IV (GIBCO ...
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bioRxiv - Developmental Biology 2024Quote: ... Cell lines were transfected with siRNAs using xTremeGene siRNA transfection reagent (1ul/well, Roche, #4476093001; standard protocol). After addition of siRNA transfection mix ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA libraries were prepared using the KAPA mRNA HyperPrep Kit (Roche) and subsequently sequenced on a NextSeq 500 sequencer (paired-end 43 base pairs ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... The FastStart Universal SYBR Green Master (Roche) was used for all reactions ...
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bioRxiv - Cancer Biology 2024Quote: ... with LightCycler® 480 SYBR Green I Master (Roche, cat. no:04 707 516 001) was used to perform quantitative PCR under following conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... PCRs reactions used KAPA HiFi HotStart ReadyMix (Roche) as per manufacturer’s instructions using an annealing temperature of 65.7C.
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bioRxiv - Microbiology 2024Quote: ... . Apical wash and basolateral samples were assayed following protocol instructions (Cytotoxic Detection Kit, Roche Applied Science). To calculate absolute values ...
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bioRxiv - Microbiology 2024Quote: ... L-lactate dehydrogenase (Roche Applied Science) was used to construct a standard curve that demonstrated an ample linear dynamic range (r2>0.998 ...
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bioRxiv - Microbiology 2024Quote: ... and CDT genes was determined by quantitative reverse transcriptase PCR (qRT-PCR) in a LightCycler 480 thermal cycler (Roche Diagnostics, USA). The primers specific for toxin A ...
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bioRxiv - Microbiology 2024Quote: ... spleens were collected from naïve C57BL/6 (CD45.1+) and a single cell suspension was obtained by incubation with Liberase Blendzyme 3 (Roche, Bradford, CT) and DNase (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Cycling was carried out in a Lightcycler (Roche), and GLuc mRNA levels determined by the ΔΔCt method using 18s RNA as reference gene (18s F ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR was performed using the Luna Universal One-Step RT-qPCR Kit (Biolaps) according to the manufacturer protocol on a LightCycler real-time PCR thermocycler (Roche). The analysis of the infection rate based on PCR data was done with the binGroup package [42] in R software.
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bioRxiv - Molecular Biology 2024Quote: Ni-NTA Magnetic Agarose Beads were incubated with 1.5µg of recombinant His-TIRR(8) in binding buffer (50mM Tris pH 7.4, 150mM NaCl, 5mM MgCl2, 10% Glycerol, Protease inhibitors (EDTA free, Roche)) for 1 hour at 4°C on a wheel ...
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bioRxiv - Molecular Biology 2024Quote: ... The digoxigenin (DIG)-labeled probes were generated by PCR on H26 genomic DNA using primers and the PCR DIG labeling Mix from Roche. Probe hybridization was detected using the DIG Luminescent Detection kit (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were lysed in lysis buffer (50mM Tris pH 7.5, 150mM NaCl, 1mM EDTA, 5mM MgCl2, 0.5% NP40, Protease and Phosphatase inhibitors (Roche), Pierce Universal Nuclease (Thermo) ...
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bioRxiv - Molecular Biology 2024Quote: ... Adapters were ligated by adding 15 µL of the ligation mix (3 µL T4 Ligase Buffer 10X, 0.5 µL T4 ligase (5 U/µL, Roche, 10799009001), 0.25 µl of x-Gene Stubby Adapter 50 mM (IDT) ...
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bioRxiv - Immunology 2024Quote: ... using a MagNA Lyser instrument (Roche Diagnostics). Serial dilutions of homogenized organs were plated on LB agar and enumerated the following day ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM EDTA) supplemented with protease inhibitor cocktail (Roche, Cat#4693116001). The lysates concentration was quantified by the BCA protein assay kit (Thermo Fisher Science ...
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bioRxiv - Immunology 2024Quote: ... Tumours were cut into small pieces and digested with 100μg/ml DNase I (Roche) and 200 U/ml Collagenase IV (Worthington Biochemical ...
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bioRxiv - Molecular Biology 2024Quote: ... completed with protease and phosphatase inhibitors (Roche #11697498001 and #5892970001), to isolate the nuclei from the cytoplasm ...
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bioRxiv - Molecular Biology 2024Quote: ... qPCR reactions were carried out on 0.625 ng of cDNA using a LightCycler 480 System (Roche), with the SYBR® Premix Ex Taq (Tli RNaseH Plus ...
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bioRxiv - Molecular Biology 2024Quote: Total RNAs were isolated using TriPure Isolation Reagent (Roche). For reverse transcription ...
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bioRxiv - Molecular Biology 2024Quote: ... which were then sealed and loaded into a LightCycler 480 differential scanning fluorimeter (Roche) equipped with a 100W xenon excitation lamp ...
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bioRxiv - Cancer Biology 2024Quote: ... human 293FT cells were grown on 10cm dishes and transfected with sgRNA-containing vector (pX330A-1x2-cNAT10/PITCh) and donor vector using Xtremegene 9 transfection reagent (Roche). 72 h after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were co-transfected with 5 μg of Myc-tagged NAT10 and 5 μg of BRD4 short or long isoform using the Xtremegene 9 DNA transfection reagent (Roche). After overnight incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were validated using the Agilent TapeStation and quantified by using Qubit Fluorometer as well as by quantitative PCR (KAPA Biosystems). The sequencing libraries were multiplexed and clustered on a flowcell ...
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bioRxiv - Molecular Biology 2024Quote: ... containing Triton(R) X-100 (Nacalai Tesque, Cat# 35501-15) and cOmplete™ protease inhibitor cocktail (Roche, Cat# 4693116001). After centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA isolation was performed using the MagNA Pure LC system (Total nucleic acid isolation kit, Roche Molecular System) or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0 ...
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bioRxiv - Microbiology 2024Quote: ... All buffers contained cOmpleteTM EDTA-free protease inhibitors (Roche). Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Anti-GFP mAbs (Roche) were used at a dilution of 1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Pelleted cells were resuspended in Lysis buffer (50mM Tris pH 7.4, 150mM NaCl, 5mM MgCl2 1% Triton X-100, protease inhibitors (Roche Complete Inhibitor cocktail ...
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bioRxiv - Cell Biology 2024Quote: ... protease inhibitors (Roche Complete Inhibitor cocktail, Roche, Switzerland)) and incubated for 20 min at 4°C on a rotator ...
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bioRxiv - Cell Biology 2024Quote: ... and Complete Protease Inhibitor (Roche) for 30 min with gradual agitation before clarification of samples by centrifugation at 10,000 g for 10 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Cells were resuspended in 400 μL of lysis buffer (50 mM Tris-HCl [pH 8.0], 150 mM NaCl, 1% NP-40, cOmplete EDTA-free protease inhibitor tablets [Roche, Basel, Switzerland]), incubated for 1 h at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then resuspended in 10 ml of cold PBS and incubated with cOmplete mini EDTA-free protease inhibitor (Roche, 11836170001) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and PhosSTOP (Roche). Protein concentration was calculated by using the BCA Assay (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... Schizonts were then lysed in the presence of 0.15 % saponin in PBS containing cOmplete Mini EDTA-free Protease and PhosSTOP Phosphatase inhibitor cocktails (Roche) for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... Plate-based DNA library preparation for Illumina sequencing was performed on the PerkinElmer Sciclone NGS robotic liquid handling system using Kapa Biosystems library preparation kit (Roche). 200 ng of sample DNA was sheared to 300 bp or 600 bp using a Covaris LE220 focused-ultrasonicator ...
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bioRxiv - Microbiology 2024Quote: ... The prepared libraries were then quantified using KAPA Illumina library quantification kit (Roche) and run on a LightCycler 480 real-time PCR instrument (Roche) ...