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Citations for Roche :
501 - 550 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... quantitated by qPCR using KAPA Library Quantification Kit (Kapa Biosystems, Inc., Wilmington, MA, USA), and validated using Agilent High Sensitivity DNA Kit on a Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... cOmplete EDTA-free protease inhibitor cocktail (Roche), phosphate inhibitors P1 and P2 (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... Mini Protease Inhibitor Cocktail (Roche; 11836153001) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... and 1x protease inhibitor cocktail (c0mplete EDTA-free, Roche). Cells were lysed using a continuous-flow homogenizer (Avestin ...
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bioRxiv - Cancer Biology 2024Quote: qRT-PCR was performed in triplicates in 384-well plates using primers and Roche universal probe library (Roche, 04869877001) with TaqManTM Universal MasterMix II (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... 1x PhosStop (Roche, 4906837001) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... 1x complete protease inhibitor cocktail (Roche, 04693132001), 1x PhosStop (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... and LightCycler® 480 II (Roche). Alternatively ...
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bioRxiv - Cell Biology 2024Quote: ... and DNase I (400 U/mL, Roche). The digested tissue pieces were then filtered through a 70 µm filter ...
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bioRxiv - Cell Biology 2024Quote: ... cOmplete™ Proteasehemmer-Cocktail by Roche were used to stop protein degradation ...
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bioRxiv - Cell Biology 2024Quote: ... cells were detached using cold PBS and lysed in 350 µl lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) containing protease inhibitor (Roche) for at least 20 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was blotted with anti-GFP monoclonal primary antibody (mouse monoclonal, Sigma- Roche #11814460001) at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... containing 5% beta-mercaptoethanol and protease and phosphatase inhibitors (Roche Complete Mini protease inhibitor ...
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bioRxiv - Cell Biology 2024Quote: ... containing 5% beta-mercaptoethanol and protease and phosphatase inhibitors (Roche Complete Mini protease inhibitor, Sigma-Aldrich #11836153001, and Roche Complete Ultra phosphatase inhibitor ...
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bioRxiv - Cell Biology 2024Quote: ... The quantitative real-time PCR was done using Maxima SYBR Green/ROX qPCR Master Mix (2X) (Fermentas) and LightCycler 480 (Roche) quantitative PCR machine ...
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bioRxiv - Cell Biology 2024Quote: ... just after the resection the tissue was dissociated into small pieces (∼2 mm × 2 mm × 2 mm) and then digested for 30 minutes at 37°C with Collagenase (2 mg/mL, Roche, Basel, Switzerland) and DNase I (400 U/mL ...
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bioRxiv - Biophysics 2024Quote: ... 20 mM imidazole) supplemented with 3 protease inhibitors (Roche) and then lysed by an EmulsiFlex-C3 (Avestin ...
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bioRxiv - Biophysics 2024Quote: ... 1 tablet of cOmplete ULTRA EDTA-free protease inhibitors (Roche Applied Science) (pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... 5 μM LMD-009 and EDTA-free protease inhibitor cocktail tablets (Roche). Then the suspension was incubated at room temperature for 1.5 h after adding apyrase (25 mU/mL ...
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bioRxiv - Biophysics 2024Quote: ... 5% glycerol) per 1 L of cells with one EDTA-free protease inhibitor cocktail tablet (Roche). Cells were lysed using a Misonix Sonicator 3000 (110 W for 2 min total ON-time ...
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bioRxiv - Biophysics 2024Quote: ... EDTA-free protease inhibitor cocktail (Roche Pharmaceuticals, Basil, Switzerland). Cells were lysed by three passes through an Avestin Emulsiflex C3 (Bee International ...
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bioRxiv - Biophysics 2024Quote: ... 10 mM imidazole pH 7.0) supplemented with 0.01% Nonidet P40 (NP40) substitute (Roche). The polymerized F-actin was stored at 4 °C overnight before grid preparation.
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bioRxiv - Cancer Biology 2024Quote: ... and then fragmented to around 200-300 bp with heat in fragmentation buffer (KAPA RNA Hyper+RiboErase HMR, Roche). Double-stranded cDNA was then synthesized by reverse transcription and underwent end-repair and ligation using New England Biolabs (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were developed by using either secondary antibodies linked to horseradish peroxidase with the UltraView™ Universal DAB Detection Kit (Ventana Medical System, Roche) or secondary antibodies linked to fluorophores ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was diluted 1:3 and qRT-PCR was conducted using a SYBR Green master mix (Roche, FSUSGMMRO) on a Light Cycler 480 (Roche) ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions including 25U RNA Inhibitor (Roche/Merck, 3335399001) per reaction ...
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bioRxiv - Cancer Biology 2024Quote: ... 1X cOmplete Protease Inhibitor Cocktail (Roche) and 20U/mL RNAsin Ribonuclease Inhibitors (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were generated with a KAPA Hyper Kit (Roche), incorporating adaptors and index primers provided by NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1X EDTA-free Complete protease inhibitor mixture (Roche)) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by primary antibody labelling (16-120 min) and signal detection carried out with the OptiView DAB IHC Detection Kit (VENTANA/Roche), as described previously (33) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% Triton X-100) supplemented with 1X cOmplete Protease Inhibitor Cocktail (Roche), 1X PhosSTOP (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 mM EDTA) supplemented by a cocktail of protease (04693116001, Roche) and phosphatase inhibitors (88667 ...
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bioRxiv - Cell Biology 2024Quote: ... with glass coverslip (coated with 5 μg/cm2 fibronectin (Roche) for an hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... cell nuclei were counterstained using 40,60-diamidino-2-phenylindol (DAPI, Roche, Mannheim, Germany). Labeled cells were washed three times with TBS before being covered with Fluoromount mounting medium (Southern Biotech ...
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bioRxiv - Cell Biology 2024Quote: ... GFP was blocked with anti-GFP antibody (Roche) and ubiquitin was blocked with anti-ubiquitin was detected using an anti-GFP antibody (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... and ubiquitin was blocked with anti-ubiquitin was detected using an anti-GFP antibody (Roche) and ubiquitin was detected using an anti-ubiquitin antibody (Santa Cruz) ...
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bioRxiv - Cell Biology 2024Quote: ... After washes, the cells were stained with the DNA stain DAPI (4, 6 diamidino-2-phenylindole dihydrochloride) (Roche, #1023627001) and mounded with Prolong gold anti-fade mound media (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed with 1X PBS and were resuspended in 1 mL of 1X Triton Lysis Buffer (50mM Tris pH 7.5, 150mM NaCl, 0.5% Triton and cOmplete, EDTA-free Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Cell Biology 2024Quote: ... Blood glucose was quantified using an ACCU-CKEK glucometer (Roche, Germany) following tail vein-puncture of whole blood sampling ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP sequencing libraries were constructed using the KAPA Hyper Prep Kit (Roche; cat. 08278539001) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and no degradation peaks) were quantified using the Library Quantification Kit (Roche; cat. 07960298001). Finally ...
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bioRxiv - Cancer Biology 2024Quote: ... size and concentration of cDNA libraries were checked using the High Sensitivity NGS kit Fragment Analyzer and qPCR (ROCHE Light Cycler 480). Libraries were sequenced using an Illumina Novaseq 6000 sequencer as single-end 100 base reads ...
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bioRxiv - Cancer Biology 2024Quote: IHC staining was performed using a Ventana BenchMark ULTRA IHC/ISH System (Roche, Rotkreuz, Switzerland). For both the sarcoma xenograft TMA and human sarcoma tissue samples ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred to PVDF membranes (Roche). The membranes were blocked in 5% non-fat milk (Difco ...
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bioRxiv - Cancer Biology 2024Quote: ... FastStart Universal SYBR® Green Master (ROX) (#0491385001, Roche, Mannheim, Germany) was used to perform the quantitative PCR assay on the 7900HT Sequence Detection System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... mitochondrial dehydrogenase activity was assayed using the WST-1 reagent (Roche Applied Science Ltd, Hertfordshire, UK) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was extracted using the High Pure FFPET DNA Isolation Kit (Roche). Extracted FFPE DNA was repaired using the NEBNext FFPE DNA Repair Mix ...
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bioRxiv - Cell Biology 2024Quote: Cells were harvested and lysed in RIPA buffer (Roche) containing 1x Halt Protease Inhibitor Cocktail (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... and 1x PhosStop (Roche, 4906837001), clarified at 13,000 g 4°C for 15 minutes ...