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Citations for Roche :
101 - 150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 10 mM NaF) supplemented with protease inhibitors (1x Complete® EDTA-free (Roche, Cat. # C7698), 1 mM PMSF ...
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bioRxiv - Cell Biology 2024Quote: ... 1.0% Triton X-100) supplemented with protease inhibitors (1x Complete® EDTA-free (Roche, Cat. # C7698), 1 mM PMSF ...
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bioRxiv - Cell Biology 2024Quote: ... The remaining fraction was diluted with 720 µL IP buffer supplemented with protease inhibitors (1x Complete® EDTA-free (Roche, Cat. # C7698), 1 mM PMSF ...
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bioRxiv - Cell Biology 2024Quote: Cells or tissues were washed with PBS and lysed in an appropriate amount of protein lysis buffer (TritonX100 -1%, Tris 7.4 -50mM, EDTA -5mM, NaCl -150mM, and glycerol -5%) containing Protease Inhibitor Cocktail (Roche). We used Phosphatase Inhibitor Cocktail (Millipore ...
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bioRxiv - Cell Biology 2024Quote: All qPCR reactions were performed with a LightCyler 1.3 Real-Time PCR system (Roche S/N: 140 6143). The MgCl2 concentration (1-3 mM ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time qPCR with SYBR Green Master Mix (Roche) was performed to evaluate AAV9 titers.
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bioRxiv - Cell Biology 2024Quote: ... 100 µM random hexamer primers (Roche # 11034731001) and 1 μl RNAsin Plus Inhibitor (Promega #N2615 ...
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bioRxiv - Cell Biology 2024Quote: ... a final concentration of 1 ng/μl cDNA was mixed with 2 μl FastStart DNA Master SYBR Green I (Roche #03 003 230 001), 0.5 μM forward and reverse primer ...
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bioRxiv - Neuroscience 2024Quote: ... 1x Proteinase inhibitors (Complete, Roche)) ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% SDS and protease inhibitor (Complete, Roche). After centrifugation at 14,000 rpm for 10 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were selected using 250 µg/mL hygromycin (10843555001; Roche). 3T3-L1 fibroblasts were differentiated into mature adipocytes as previously described (38 ...
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bioRxiv - Cancer Biology 2024Quote: ... consisting of 2 μL 5X KAPA HiFi HotStart Buffer (Roche, KK2502), 0.048 μL of 10 mM dNTPs ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.2 μL of 10 mM dNTPs (Roche, 50-196-5273), 0.02 μL Protector RNase inhibitor (Sigma Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... supplemented with 0.4% protease inhibitor cocktail (Roche, Cat# 04693132001), 0.5% β-mercaptoethanol (PanReac AppliChem ...
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bioRxiv - Plant Biology 2024Quote: The pulverized tissue was incubated in 30 mL of buffer 1 (400 mM sucrose, 10 mM Tris-HCl pH 8.0, 10 mM MgCl2, 5 mM β-mercaptoethanol, 1 mM PMSF, 1× Roche cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2024Quote: ... completed with cOmplete protease inhibitor (1/30) (Roche) and centrifugated (3,800 rpm ...
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bioRxiv - Plant Biology 2024Quote: ... using a Light Cycler 480 and Light Cycler 480 SYBR green I Master mix (Roche) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5% (w/v) Na deoxycholate and 1x protease inhibitor mix (Roche). Cell debis was pelleted by centrifugation (30,000 x g ...
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bioRxiv - Physiology 2024Quote: ... and digested in PBS with 10 mg/ml BSA (Cat#A600332-0100, BBI Life Sciences) and 10 mg/mL collagenase (Cat#11088858001, Roche) for 60 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... six reverse primers (10 μM) and Robust DNA polymerase from the KAPA2G Robust PCR Kit (Kapa Biosystems, Wilmington, MA). Cycling conditions were ...
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bioRxiv - Neuroscience 2024Quote: ... cDNAs and final libraires were run on an Agilent 4200 TapeStation to check for quality and were quantified using KAPA Library Quantification Kit (Roche, Basel, Switzerland).
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bioRxiv - Microbiology 2024Quote: ... cells were harvested by centrifugation and lysed by sonication in native buffer including Complete Mini (Roche) and Lysozyme (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... 5 µg/mL DNAse and protease inhibitors (Roche) and lysed by sonication ...
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bioRxiv - Microbiology 2024Quote: ... 5 µg/mL DNAse and protease inhibitors (Roche). Lysate was incubated with 1% N-Dodecyl-β-D-maltoside for 1 hour at 4°C with mixing and then spun down at 20,000g for 20 minutes at 4°C to remove cell debris ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in twice their volume of buffer A (50 mM HEPES pH 7.5, 500 mM KCl) with 5 mM MgCl2 and DNase I (Roche, Basel, Switzerland). The cell suspension was treated with a Sonopuls GM200 sonicator (BANDELIN electronic GmbH & Co ...
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bioRxiv - Physiology 2024Quote: ... TUNEL staining was performed using a commercial kit (in situ Cell Death Detection Kit, TMR red – Roche, Switzerland). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... 0.001 % SDS and protease inhibitor cocktail (cOmplete Mini, Roche). Three replicates were assessed for each shRNA sequence tested ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was sonicated in homogenization buffer (1% SDS, 50 mM Tris pH 7.4, 2 mM EDTA, protease and phosphatase inhibitors (Roche 11873580001; Roche 04906837001), heated at 95° C for 5 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... and protease inhibitor (cOmplete, Roche) to maintain the integrity of the proteins during further processing ...
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bioRxiv - Plant Biology 2024Quote: ... and protease inhibitor (cOmplete, Roche). Thylakoids were isolated using a discontinuous three-step sucrose gradient (2.3 M ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand complementary DNA synthesis was performed using 1 µg of total RNA using an anchored oligo (dT) and Transcriptor Reverse Transcriptase (Roche) following the manufacturers’ protocol ...
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bioRxiv - Plant Biology 2024Quote: ... and using the SYBR Green I Master mix (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... a collagenase solution from Roche was added to the µ-Slide 8 well and incubated for 15 minutes to release T cells from the collagen gel ...
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bioRxiv - Neuroscience 2024Quote: ... 0.15M NaCl) supplemented with protease inhibitor (Roche miniComplete) and PhosSTOP phosphatase inhibitor (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... and PhosSTOP phosphatase inhibitor (Roche). Protein samples of equal amounts were loaded into TGX precast gels (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The real time PCR program of the quantitative PCR (LightCycler480 II, Roche, Basel, Switzerland) was arranged as follows ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5μl SYBR Green Mastermix (Roche FastStart Universal ...
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bioRxiv - Neuroscience 2024Quote: ... sensory ganglia were dissected and incubated in warmed Collagenase P (Roche) in Hanks calcium-free balanced salt solution (HBSS ...
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bioRxiv - Neuroscience 2024Quote: ... embryos were incubated with AP-conjugated-anti-DIG antibodies (Roche, Basel, Switzerland) and stained with NBT/BCIP (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... pH 7.6) containing protease inhibitor (Roche Diagnostics, catalog #11836153001) and phosphatase inhibitor (Roche Diagnostics ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... 50 µL KAPA HiFi HS RM (Roche, KK2601). The PCR was performed with the following cycle numbers ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... 50 µL KAPA HiFi HS RM (Roche, KK2601) in a 100 µL reaction ...
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bioRxiv - Systems Biology 2024Quote: ... and 0.02 U/uL KAPA HiFi DNA Polymerase (Roche) in a 30uL volume ...
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bioRxiv - Systems Biology 2024Quote: ... 15uL of the first round products were mixed into a second PCR reaction that contained 1x KAPA HiFi GC Buffer (Roche), 300uM dNTPs ...
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bioRxiv - Systems Biology 2024Quote: ... Libraries prepared using the KAPA Library Preparation Kit (KAPA Biosystems) and amplified using NEB indexed adapters with 12 PCR cycles (15 sec at 98°C ...
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bioRxiv - Systems Biology 2024Quote: ... and adapter ligation were all performed in lo-bind tubes using the KAPA library preparation kit (KAPA biosystems). Library was amplified using the HiFi HotStart ReadyMix (KAPA Biosystems ...
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bioRxiv - Systems Biology 2024Quote: ... Library was amplified using the HiFi HotStart ReadyMix (KAPA Biosystems) as well as the universal forward primer and barcoded reverse primer and incubated following the PCR program ...
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bioRxiv - Neuroscience 2024Quote: ... titer was determined at a concentration of 1.1013 gcp/ml by quantitative real-time PCR using the Light Cycler 480 SYBR green master mix (Roche, cat # 04887352001) with primers specific for the AAV2 ITRs (fwd 50-GGAACCCCTAGTGATG GAGTT-3’ ...
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bioRxiv - Neuroscience 2024Quote: ... 0.01 M Tris-HCl pH 7.4] containing protease inhibitors (Roche, Switzerland). For sarkosyl preparations ...
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bioRxiv - Neuroscience 2024Quote: ... and complete mini EDTA-free protease inhibitor cocktail (Cat No. 11836170001, Roche) at room temperature for about 30 sec until the heads were no longer intact ...