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Citations for Roche :
451 - 500 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 8 M Urea and 1x cOmpleteTM EDTA-free protease inhibitor (Roche #11873580001). Lysates were sonicated with a probe sonicator and cleared via centrifugation at 15,000x g for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... 10% (v/v) glycerol and 1× Halt or 1x cOmpleteTM EDTA-free protease inhibitor cocktail (Roche #11873580001). Lysates were sonicated with a probe sonicator and cleared via centrifugation at 15,000x g for 5 min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... was washed once with 80 mL of lysis buffer 1 (20 mM Tris-HCl, pH 8.0) containing 2 mL of EDTA-free protease inhibitor mixture (Roche Applied Science, Penzberg, Germany). Cells were disrupted by passing the cell suspension through a Constant Cell disruption system (TS benchtop ...
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bioRxiv - Biochemistry 2024Quote: ... containing protease inhibitor cocktail (Roche; 11-873-580-001) and phosphatase inhibitor (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... and phosphatase inhibitor (Roche; 4-906-837-001) on ice for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... we mixed 0.6 µg of yeast tRNA (Roche), 10 mM MgCl2 ...
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bioRxiv - Biochemistry 2024Quote: ... and RNA was released from the membrane using Proteinase K (Roche, 03115828001). RNA was purified using neutral phenol/chloroform/isoamylalcohol (Ambion ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM magnesium chloride and protease inhibitors (Roche). A FastPrep-24 (MP Bio ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) supplemented with 1x protease inhibitor cocktail (Roche). Lysate was spun at 8,000 x g for 5 minutes at 4°C in a JA 25.5 rotor ...
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bioRxiv - Biochemistry 2024Quote: ... sensors were moved into binding buffer with 1 mM GTP (Roche), 1 mM XTP (TriLink Biotechnologies) ...
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bioRxiv - Biochemistry 2024Quote: ... and 2x cOmplete protease inhibitor cocktail (PIC) tablet (Roche, Basel, Switzerland) was added per 6 grams of cell weight and frozen via a ‘popcorn’ method (frozen drop by drop in liquid nitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... 1x protease inhibitor cocktail (Roche), and 0.1 mM PMSF at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... 250 mg of powder was thawed at 4°C followed by resuspension in 1 mL of HIP buffer (40 mM HEPES-KOH pH 7.5, 110 mM KOAc, 2 mM MgCl2, 1% Triton X-100, 0.1% Tween, 1x protease inhibitor cocktail [Roche], 1% solution P, 1 mM DTT). The lysate was next passed through a Whatman 25 mm GD/X Disposable filter (Cat No ...
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bioRxiv - Biochemistry 2024Quote: ... and resuspended in 1 mL per gram of pellet of resuspension buffer (1.2% PVP-40, 20 mM HEPES pH 7.4, 1x protease inhibitor cocktail [Roche] ...
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bioRxiv - Biochemistry 2024Quote: ... 1x protease inhibitor cocktail (Roche), and 0.1 mM PMSF ...
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bioRxiv - Biochemistry 2024Quote: ... and resuspended in 1 mL (per gram of pellet) resuspension buffer (1.2% PVP-40, 20 mM HEPES pH 7.4, 1x protease inhibitor cocktail [Roche] ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Reactions were performed in 20 μl volumes including 1x KAPA SYBR® FAST qPCR Master Mix (Kapa Biosystems), 3 pmol of each primer (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... Pre-hybridization was carried out at 48°C for 2 hours in DIG Easy Hyb buffer (Roche). The trnI/A probe was denaturalized for 20 minutes at 68°C and used for hybridization overnight at 48°C ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The qPCR ran on a LightCycler 480 System (Roche) with KAPA SYBR Fast mastermix (Sigma-Aldrich).
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bioRxiv - Microbiology 2024Quote: ... and a LightCycler 96 instrument (Roche). The number of gut symbionts was calculated based on a calibration curve for the opcP gene consisting of ten-fold dilutions of 107 to 10 copies of the PCR DNA fragment ...
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bioRxiv - Microbiology 2024Quote: ... the Fast Start Essential DNA Green Master qPCR Kit (Roche), and a LightCycler 96 instrument (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... 1 × proteinase inhibitor and phosphatase inhibitor (4693116001 and 04906845001, Roche)] ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... We prepared RNA libraries using the KAPA mRNA HyperPrep Kit and Unique Dual-Indexed Adaptors (KAPA Biosystems; Massachusetts, USA), with 200 ng of RNA as input ...
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bioRxiv - Biochemistry 2024Quote: ... real-time PCR was performed using SYBR Fast qPCR Master Mix (Kapa Biosystems) and the CFX-96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2024Quote: ... excluding mitochondrial targeting sequence) were amplified by polymerase chain reaction (PCR) using Kapa HiFi Hotstart ReadyMix (Roche) and inserted into a pET-SUMO expression vector containing an N-terminal hexahistidine-SUMO tag (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... 5mM EDTA supplemented with complete EDTA-free protease inhibitor cocktail (Roche) and PhosSTOP (Millipore) ...
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bioRxiv - Biochemistry 2024Quote: ... Frozen cell pellets were lysed in immunoprecipitation lysis buffer (50 mM Tris [pH = 7.5], 150 mM NaCl, 1% IGEPAL, 10% Glycerol) with cOmplete Protease Inhibitor Cocktail (Roche) and PhosSTOP Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.1% Cholesteryl Hemissucinate (CHS) (Avanti Pola lipids) supplemented with 2 tablets of cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). The resuspended pellet was homogenized manually in a 40 ml Kimble glass homogenizer (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... 200 mM NaCl and supplemented with cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). 4 tablets of protease inhibitor cocktail were added per 100ml of buffer ...
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bioRxiv - Biochemistry 2024Quote: ... 200 mM NaCl and 0.5% SMALP200 (CubeBiotech GmbH) supplemented with 2 tablets of cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). The resuspended pellet was homogenized manually in a 40 ml Kimble glass homogenizer (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... and PhosSTOP Phosphatase Inhibitor Cocktail (Roche) by probe sonication (2x 15 pulses) ...
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bioRxiv - Biochemistry 2024Quote: ... 1× protease inhibitor (cOmplete Protease Inhibitor Cocktail, Roche). After keeping the suspension on ice for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... and Complete® protease inhibitor cocktail (Roche Diagnostics, Mannheim, Germany)) ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was amplified using gene-specific primers and FastStart Essential DNA Green Master (Roche #06402712001). Cq values of genes of interest were normalized to housekeeping gene RHOA expression.
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with cOmplete (TM) protease inhibitor (Roche, 4693159001) and PhosSTOP tablet (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Faststart Essential DNA Green Master mix (Roche, Basel, Switzerland) was used to perform qPCR on a Lightcycler 96 Real-Time PCR System (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... The aqueous phase from each MaXtract tube (∼400 µl) was then transferred to 1.5 ml tubes containing 2 µl of 2 µg/µl glycogen (Roche, Cat# 10901393001), 1 ml ethanol was added ...
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bioRxiv - Cancer Biology 2024Quote: ... EDTA-free Protease Inhibitor Cocktail (Roche, Cat# 04693159001) at 200 x g for 3 min ...
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bioRxiv - Cell Biology 2024Quote: ... including a protease inhibitor cocktail (Roche, 04906845001, Basel, Switzerland) and a phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... was used to perform qPCR on a Lightcycler 96 Real-Time PCR System (Roche, Basel, Switzerland). The relative transcript abundance was normalized to Ef1α expression as the reference gene control ...
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bioRxiv - Cell Biology 2024Quote: ... and a phosphatase inhibitor cocktail (Roche, 11697498001) for 30 min followed by centrifugation and the supernatant was collected as a protein extract ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was isolated by lysing cells (10 mM Tris-HCl pH 8.0, 1 mM EDTA, 0.67% SDS, and 125 μg/ml proteinase K from Roche), followed by incubation 4 hours at 55°C with weak agitation ...
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bioRxiv - Cancer Biology 2024Quote: ... pH 7.4, 150 mM NaCl, 1.25 mM MgCl2, 5 U/ml RNase inhibitor [Invitrogen], protease inhibitor cocktail [Complete; Roche] ...
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bioRxiv - Cancer Biology 2024Quote: ... and phosphatase inhibitors PhosSTOP™ (Roche, Cat#4906837001), and cleared by centrifugation ...
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bioRxiv - Cancer Biology 2024Quote: ... Mini Protease Inhibitor Cocktail (Roche, Cat#11836153001) and phosphatase inhibitors PhosSTOP™ (Roche ...
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bioRxiv - Cancer Biology 2024Quote: Immunohistochemistry (IHC) staining was performed with a Bench-Mark ULTRA Autostainer (VENTANA/Roche) on 3 µm TMA sections ...
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bioRxiv - Cancer Biology 2024Quote: Cells were harvested and lysed with 1x RIPA buffer (CST, #9806) supplemented with 1x protease inhibitor cocktail (Roche, #693116001) and 1x phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured using the Cell Proliferation Kit I (MTT) (Roche, #11465007001) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1x phosphatase inhibitor cocktail (Roche, #04906845001) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 3mg DNase (Roche), for 1 hour at 37C ...