Labshake search
Citations for Roche :
151 - 200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... 50 µL of Kapa Hifi Hotstart Ready Mix (Roche) and 40 µL of Nuclease-free water (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... Western blot was stained with the following primary antibodies: mouse anti-GFP antibody (11814460001, Roche) and rabbit anti-HA antibody (H6908 ...
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bioRxiv - Developmental Biology 2024Quote: ... After post-hybridisation washed and blocking with BMB (Roche), embryos were treated with anti-DIG antibody coupled to alkaline phosphatase (Merck ...
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bioRxiv - Developmental Biology 2024Quote: ... TMR Red (12156792910, Roche). Slides were fixed in 4% PFA ...
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bioRxiv - Genetics 2024Quote: ... qPCR was performed using the KAPA SYBR FAST kit (KAPA Biosystems) and BioRad CFX96 Real-Time System ...
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bioRxiv - Genetics 2024Quote: 12.5 Kapa HiFi HotStart PCR ReadyMix (KAPA Biosystems, KK2601)
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bioRxiv - Genetics 2024Quote: ... and 15 μL of Kapa HiFi HotStart ReadyMix polymerase (Roche Diagnostics, KK2601). Each reaction tube was initially incubated at 95°C for 5 mins ...
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bioRxiv - Genetics 2024Quote: ... 1 mM DTT, 10 mM NaPPi, 5 mM EGTA, 5 mM EDTA, 0.1 mM Na3VO4, 5 mM NaF, and Roche cOmplete™ Protease Inhibitor Mix). Crude extracts were prepared by centrifugation ...
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bioRxiv - Genetics 2024Quote: ... and digested with NB4 collagenase (12mg/ml) (SERVA) and Dispase II (100U/ml) (Roche) for 1 hour ...
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bioRxiv - Genetics 2024Quote: ... and phosphatase inhibitors (Roche, 04906837001). Lysis was allowed to proceed on ice for 30 minutes before centrifugation at 20,000xg for 10 min at 4°C ...
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bioRxiv - Genomics 2024Quote: ... and the RT-qPCR was performed using ChamQ SYBR qRCR Master Mix (High ROX Premixed) (Cat# Q341-02-AA, Vazyme, China) by the LightCycler® 480 System (Roche, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... digested three times (DMEM high glucose with 2% FBS, 100 µg/ml DNase I, and 100 µg/ml Liberase TM, Roche) or until no tissue fragments remained ...
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bioRxiv - Microbiology 2024Quote: ... 5 μl of KAPA HiFi 2X master mix (Roche) and 3 μl of diluted overnight culture grown in 96-well plates ...
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bioRxiv - Microbiology 2024Quote: ... cDNAs were diluted to 1 ng/ µL and used for qPCR analysis in a 10 µL reaction mix containing 5 µL of LightCycler® 480 SYBR Green I Master mix (Roche), 300 nM of each primer ...
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bioRxiv - Microbiology 2024Quote: ... qPCR was performed in triplicate using a LightCycler ® 480 System (Roche) with preheating at 95 °C for 5 minutes and then 40 cycles of 95 °C for 15 s and 60 °C for 60 s ...
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bioRxiv - Microbiology 2024Quote: ... 0.1% SDS) supplemented with cOmplete Mini Protease Inhibitor Cocktail (Roche, Penzberg, Germany). Cell-free virions were further cleared of debris by centrifugation at 1000 × g for 5 min at 4°C and resuspended in RIPA buffer supplemented with cOmplete Mini Protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2024Quote: ... For library preparation the Kapa LTP Library Preparation Kit for Illumina Platforms was used (Kapa Biosystems, Part Number KK8232). The cDNA was filtered into the End Repair step of the method and the library preparation was followed as per the protocol through to Adapter Ligation ...
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bioRxiv - Microbiology 2024Quote: ... Mix (Roche) with buffer 3 or the Taq polymerase (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... with buffer 3 or the Taq polymerase (Roche). The initial denaturation for Taq polymerase at 95 °C for 5 min was followed by 30 amplification cycles of 1 min at 95 °C ...
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bioRxiv - Microbiology 2024Quote: ... 1% NP-40) supplemented with Complete Protease Inhibitor Cocktail (Roche) for 30 min on ice ...
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bioRxiv - Genetics 2024Quote: ... RNA was visualized using alkaline phosphatase conjugated anti-DIG antibody (Roche), using CBIP and NBT as substrates (Roche) ...
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bioRxiv - Genetics 2024Quote: ... they were quantified on a CFX 384 Touch Real-Time PCR Detection System using the KAPA Library Quantification Kit (KAPA Biosystems, cat. KK4824). Finally ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA prepared using the KAPA Express reagent (a component of the KAPA Mouse Genotyping Kit from Roche, 07961804001) and analyzed for both CRISPR lesions and repeat size as described below.
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bioRxiv - Genetics 2024Quote: ... Three tenths of a microliter of Robust HotStart KAPA2G polymerase (Roche, 07961316001) was added per 100 μl of PCR mix ...
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bioRxiv - Genetics 2024Quote: ... plus a protease inhibitor mixture (Roche Applied Science). Samples were resolved by SDS-PAGE and transferred to PVDF ...
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bioRxiv - Genetics 2024Quote: ... using the Kapa mRNA HyperPrep kit (Roche, Switzerland), using 500 ng of total RNA and following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Total RNA was extracted from 5-8 pairs of ovaries using an RNA extraction kit (Roche). 50 ng of total RNA was used to make cDNA using the Transcriptor First Strand cDNA Synthesis kit (Roche ...
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bioRxiv - Genetics 2024Quote: ... using CBIP and NBT as substrates (Roche). Each set of experiments was carried out with control and experimental samples in parallel to minimize experimental variation ...
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bioRxiv - Genetics 2024Quote: ... 50 ng of total RNA was used to make cDNA using the Transcriptor First Strand cDNA Synthesis kit (Roche) with random primer ...
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bioRxiv - Genetics 2024Quote: ... Real-time PCR was run in a Lightcycler 480 (Roche), using SYBR Green Master Mix–BioRad as a fluorescent dye ...
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bioRxiv - Genomics 2024Quote: ... We used 10 ng of DNA from two biological replicates to generate sequencing libraries using KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... pegRNAs were amplified from the oligo pool via PCR using KAPA HiFi HotStart ReadyMix (#KK2602, Roche). pegRNA oligo subsets were specifically amplified from the oligo pool using primers specific for the library BC (Supplementary Table 9) ...
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bioRxiv - Genomics 2024Quote: ... Cells were re-suspended in digestion buffer (50 mM Tris-HCl pH7.6, 2 mM CaCl2, 0.2% NP40) containing protease inhibitors (complete EDTA-free, Roche) and warm-up to 37°C for 2min ...
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bioRxiv - Genomics 2024Quote: Genomic DNA (5∼8 μg) for Nanopore sequencing was end-repaired and ligated via a KAPA Hyper Prep Kit (Cat#KR0961, Kapa Biosystems, Wilmington, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... but replaced the Zymo-Spin V column binding apparatus with a high pure extender assembly from the High Pure Viral Nucleic Acid Large Volume Kit (Roche 05114403001). Double-stranded Illumina libraries were prepared using the Blunt-End Single Tube (BEST ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we prepared libraries using Hyper Prep Kits (Kapa Biosystems, Inc.) following the manufacturer’s protocol at the recommended volume ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We quantified final libraries using a commercially available library quantification kit (Kapa Biosystems, Inc.), combined libraries into 10 nM pools ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we PCR-amplified the resulting libraries using HiFi HotStart polymerase (Kapa Biosystems, Inc.) and 10 PCR cycles following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... into a genomic DNA library preparation protocol (KAPA HyperPrep Kit, KAPA Biosystems, Wilmington, MA, U.S.A.). For targeted enrichment of UCEs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We incorporated sample-specific dual-indexed adapters to library fragments (Glenn et al., 2019) by PCR amplifying the resulting libraries using HiFi Hotstart ReadyMix (Kapa Biosystems, Inc.) and 8-12 PCR cycles following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were then incubated with anti-digoxigenin Fab fragments conjugated to alkaline phosphatase (Roche) diluted 1:6000 in PBT+BSA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we input 50-1,600 ng sheared DNA to a modified genomic DNA library preparation protocol (Kapa Biosystems, Inc.) that incorporated “with-bead” cleanup steps (Fisher et al. ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We verified enrichment success and measured size-adjusted DNA concentrations of each pool with qPCR using a SYBR-FASTqPCR kit (Kapa Biosystems, Wilmington, MA, U.S.A.) and a Bio-Rad CFX96 RT-PCR thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and protease inhibitors (Roche). Cells were lysed using a combination of freeze-thawing (3 cycles of dry ice at -37 °C) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or Roche High Pure™ PCR Template Preparation Kits (Roche Molecular Systems ...
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bioRxiv - Developmental Biology 2024Quote: ... ST-HSCs and LT-HSCs using a KAPA RNA HyperPrep kit with RiboErase (HMR) (Kapa Biosystems) and sequenced using a Hiseq 1500 (Illumina ...
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bioRxiv - Developmental Biology 2024Quote: ... Cell nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL, Roche). To reduce autofluorescence ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was performed using KAPA Hyper Prep (Roche, Basel, Switzerland) following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2024Quote: ... and protease inhibitor cocktail (Roche, 11697498001). Cell lysis was facilitated with 27G needle and syringe at least 7 times ...
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bioRxiv - Genetics 2024Quote: ... Complete Phosphatase inhibitor cocktail (Roche, 11836145001), 10mM sodium fluoride ...