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Citations for Roche :
2501 - 2550 of 3182 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: Expression of GFP was analyzed in automiG induced cells by western blotting using mouse anti-GFP (Roche®) and anti-Mbf1 antibodies (43 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HCC827 and H661 cells were transfected with the siRNAs using X-tremeGENETM siRNA Transfection Reagent (Roche, Mannheim, Germany) according to the manufacturer’s manual when the cells reached a confluence of 60-80% ...
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bioRxiv - Immunology 2019Quote: ... NK92 cells were maintained in Myelocult medium supplemented with penicillin/streptomycin and 100 U/ml IL-2 (Roche). CRISPR-edited cell lines were generated by nu-cleofecting NK92 cells or CD34+ precursors with pCMV-Cas9-GFP all-in-one plasmid (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... All three vectors were transfected into DLD-1 TIR1 cells using Xtreme Gene 9 DNA transfection reagent (Roche). Cells were sorted based on their YFP fluorescence and single clones were isolated ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed with an ice-cold Lysis/Wash Buffer supplemented with fresh protease and phosphatase inhibitors (Roche). Cell lysates were pre-cleared by incubating the lysate with Control Agarose Resin at 4°C for 1 h ...
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bioRxiv - Immunology 2020Quote: Single cell suspensions of the tumor were obtained after tumor digestion with 400U/mL of collagenase D (Roche) at 37C for 1 hour ...
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bioRxiv - Cell Biology 2019Quote: ... Drug cytotoxicity was evaluated by the colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay (Cell Proliferation Kit I, Roche) with a NanoQuant microplate reader (Tecan Trading AG ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell proliferation was assessed by WST-1 assay as per manufacturer’s protocol (Roche Applied Science, Indianapolis, IN, USA). Colony formation assay was performed with MethoCult methylcellulose-based media as per manufacturer’s protocol (Stemcell Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were resuspended in lysis buffer (8M urea, 50mM EPPS pH 8.5, 150mM NaCl, Roche protease inhibitor tablet) and lysed by bead beating ...
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bioRxiv - Cell Biology 2020Quote: ... CMV-GFP-LC3 stable HeLa cell line was cultured as described above with G418 (0.1 mg/ml, Roche). PC12 (rat pheochromocytoma ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed with 10ml of cold 1x PBS supplemented with proteinase inhibitors and phosphatase inhibitors (Roche # 4693132001) and the pellets were snap frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The cell culture supernatants containing the retroviruses were harvested 48 hours later and premixed with DOTAP (Roche, Switzerland) before spin transfection at 2400 rpm and 32°C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was harvested from cells using a High Pure RNA Isolation Kit (Roche Diagnostics Indianapolis, IN, USA), according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... cells or tissues were collected and lysed in RIPA lysis buffer supplemented with Protease Inhibitor Cocktail (Roche, Germany) for 15 min on ice ...
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bioRxiv - Cancer Biology 2021Quote: For HA pulldown assays the cells were lysed with 500 µL of IP Lysis Buffer containing cOmplete (Roche) protease inhibitor cocktail and incubated at 4°C for 35 min on a rotator followed by centrifugation at 15000 rpm for 15 min at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: On day 0 (start of differentiation) human pluripotent stem cells were treated with 1mg/ml Collagenase B (Roche) for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... resuspended in two packed cell volumes of TMG(300) plus supplements (complete EDTA-free protease inhibitor cocktail (Roche), 0.5 mM PMSF ...
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bioRxiv - Developmental Biology 2020Quote: ... nuclei were harvested using cell lysis buffer (5 mM PIPES, pH 8.0, 85 mM KCL, 0.5% NP40, and Roche cOmplete ...
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bioRxiv - Cancer Biology 2021Quote: Cells were lysed with RIPA buffer containing a complete protease and phosphatase inhibitor tablet (Roche #11836153001, #PHOSS-RO). Lysates were cleared by centrifugation at 14,000xq for 15 min at 5°_C ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA was extracted from cells using High pure RNA isolation kit from Roche (Ref. 11828665001, lot. 38800800) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and cell suspensions were prepared by digesting the tissue in a cocktail consisting of Liberase TL (Roche, 5401020001) and DNase I (Sigma ...
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bioRxiv - Microbiology 2020Quote: Isolation of intracellular RNA was performed by lysing infected cell monolayers with TriPure isolation reagent (Roche Applied Science) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were harvested by trypsinization and immediately lysed in RIPA lysis buffer containing cocktails of proteases inhibitors (Roche) and phosphatase inhibitors (Sigma) ...
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bioRxiv - Microbiology 2020Quote: Isolation of intracellular RNA was performed by lysing infected cell monolayers with TriPure isolation reagent (Roche Applied Science) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... The concentration of each 10x single cell and V(D)J library was determined through qPCR (Kapa Biosystems) to produce cluster counts appropriate for the NovaSeq 6000 platform ...
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bioRxiv - Cancer Biology 2019Quote: ... Heat induced epitope retrieval was applied for 1 hour using cell conditioning 1 buffer (CC1, Roche, #950-500) followed by the application of anti-CD31 (Histobiotec ...
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bioRxiv - Developmental Biology 2022Quote: The TUNEL assay was performed in cryosectioned lung tissue using the In Situ Cell Death Detection Kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The next day cells were transfected using X-tremeGENE HP DNA transfection reagent (Roche 06-366-236-001) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were lysed with lysis buffer B without DTT supplemented with cOmplete™ Protease Inhibitor cocktail (Roche) and PhosSTOP (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were lysed with Cell Lysis Buffer (10mM Tris-Cl pH 8, 10mM NaCl, 0.5% NP40, 1xPIC (Roche)) followed by mechanical shearing for 10minutes on ice ...
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bioRxiv - Cell Biology 2022Quote: Cells or tissue powders were directly dissolved in 1 ml of the TriPure Isolation Reagent (Cat. 11667165001, Roche) and extracted using a column-based kit (Cat ...
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bioRxiv - Cell Biology 2022Quote: ... Cells where lysed in immunoprecipitation buffer (50 mM Tris-HCl, 0.5% NP-40, and Roche protease inhibitor cocktail) and subjected to GFP trap (ChromoTek) ...
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bioRxiv - Cell Biology 2022Quote: ... the whole-cell extracts and immunoprecipitates were subjected to western blotting using monoclonal anti-GFP (JL-8, Roche), Flag-M2 monoclonal antibody (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then stained using the in situ TMR red cell death detection kit (Roche Applied Science, cat#: 12156792910) [39] ...
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bioRxiv - Neuroscience 2023Quote: Cell apoptosis of brain tissues around the hematoma was measured with a TUNEL Apoptosis Assay Kit (Roche, 11684817910) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Cells were washed in PBS and lysed in RIPA buffer (Pierce) supplemented with cOmplete protease inhibitor cocktail (Roche). For experiments investigating MATR3 phosphorylation ...
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bioRxiv - Neuroscience 2022Quote: Cells were lysed in ice-cold RIPA buffer supplemented with cOmplete mini protease inhibitor cocktail (Cat.#1183615300, Roche) and PhosSTOP (PHOSS-RO ...
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bioRxiv - Microbiology 2022Quote: ... Lamina propria-resident immune cells from large intestine were isolated by digesting intestinal tissue with Collagenase D (Roche), Collagenase V (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed and lysed with Lysis Buffer in the presence of 1X Protease Inhibitor Cocktail (Roche,11873580001). Chromatin was fragmented into small pieces (200-500 bp ...
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bioRxiv - Molecular Biology 2024Quote: ... Pellets were resuspended in 500 µL cold cell lysis buffer (5 mM PIPES pH8.0, 85 mM KCl, 0.5% NP-40, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min on ice) ...
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bioRxiv - Genetics 2023Quote: ... Cells were incubated overnight in primary antibodies targeting GFP to stain GFP labelled CTCF or RAD21 (Roche, 11814460001) or SON (abcam ...
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bioRxiv - Physiology 2024Quote: ... TUNEL staining was performed using a commercial kit (in situ Cell Death Detection Kit, TMR red – Roche, Switzerland). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the apoptosis of MIN6 cells in each group was assessed using TUNEL apoptosis detection kit (Roche, Basel, Switzerland). Briefly ...
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bioRxiv - Cell Biology 2024Quote: Cell lysates from mouse tissues were prepared in RIPA buffer (Beyotime, P0013B) containing Protease Inhibitor Cocktail (Roche, 11873580001) and 1mM PMSF ...
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bioRxiv - Cell Biology 2023Quote: ... or MDCK-Ecad Res cells were pre-treated with Mitomycin C at a concentration of 10μg/ml (Roche) for a duration of 1 hour to inhibit cell proliferation ...
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bioRxiv - Microbiology 2023Quote: ... Upon reaching OD600= 0.3 total RNA was extracted from cells using a High Pure RNA Isolation Kit (Roche). Samples at different time points were centrifuged for 5 min at 2,000 relative centrifugal force and pellets were flash-frozen in liquid nitrogen ...
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LptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicrybioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 20 mM Tris-HCl pH 8 containing a EDTA-free protease inhibitor cocktail (Roche). For the purification of protein samples that had to be analyzed both by reducing and non-reducing SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were stained using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL apoptosis assay kit, Roche, American), and the cells were viewed under a fluorescence microscope.
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bioRxiv - Genomics 2022Quote: Crosslinked cells were incubated in Cell Lysis Buffer (10 mM HEPES pH 7.5, 10 mM NaCl, 0.2% IGEPAL) + Protease Inhibitors (Roche) for 5 minutes on ice ...
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bioRxiv - Genomics 2022Quote: ... Cells were collected in 1xPBS supplemented with 1X complete EDTA-free protease inhibitor cocktail (PI tablets, 5056489001, Roche) on ice ...