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Citations for Roche :
2451 - 2500 of 3023 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended in lysis buffer supplemented with 1 mM PMSF and cOmplete EDTA-free protease inhibitor cocktail (Roche) and lysed by sonication ...
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bioRxiv - Cell Biology 2020Quote: ... culture media were collected and cells harvested on ice in Nonidet lysis buffer supplemented with protease inhibitor mixture (Roche). Culture media and cell lysates were precleared and α1AT was immunoprecipitated with a α1AT polyclonal antibody (total ...
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bioRxiv - Cell Biology 2020Quote: Genomic DNA was extracted from sorted cells by first decrosslinking cells in a solution of 10 mM Tris pH 7.5 and 10 mg/mL Proteinase K (Roche) in PBS at 55°C for 24 hours ...
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bioRxiv - Genomics 2020Quote: ... all multiplexed single-cell libraries (n = 30) were quantified using the KAPA Library Quantification Kit for Illumina Platforms (Roche) and pooled in an equimolar ratio ...
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bioRxiv - Biophysics 2021Quote: ... The cell pellet was resuspended in 1 ml of polysome lysis buffer supplemented with EDTA-free protease inhibitors (Roche) and was grounded in liquid nitrogen using an RNase-free mortar and pestle ...
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bioRxiv - Microbiology 2021Quote: ... Cells were resuspended in TBSI (50 mM Tris, 100 mM NaCl, pH 8, plus Complete Protease Inhibitor Cocktail, Roche) and broken using 0.1 mm silica spheres (Lysing Matrix B ...
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bioRxiv - Developmental Biology 2020Quote: ... Neural stem / progenitor cells were seeded in coated 24-well plates (1.6×105 cells per well) and transfected with the reporter plasmids using xTremeGENE HP DNA transfection reagent (Roche). Cells were collected 48hr after transfection using Passive lysis buffer (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were then harvested on ice lysed with M2-Lysis buffer72 supplemented with phosphatase and protease inhibitors tablets (Roche), and lysates ran on 15% SDS-PAGE at constant voltage (75V for fifteen minutes followed by 90V for 2 hours) ...
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bioRxiv - Molecular Biology 2021Quote: ... After 24 h cells were lysed in lysis buffer (0.5 (v/v))% NP-40 in PBS supplemented with protease inhibitors (Roche) and phosphatase inhibitors (Roche) ...
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bioRxiv - Immunology 2019Quote: HeLa or Jurkat cells were seeded on 13mm diameter glass coverslips and transfected the next day using Fugene (Roche). Cells were fixed 24 h later with 3% paraformaldehyde ...
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bioRxiv - Biochemistry 2019Quote: ... at 37°C and 5% CO2 were seeded onto ibidi μ-slides at 30,00 cells/well and transfected with TDP-43RRM-GFP reporter constructs (300 ng) using X-tremeGENE 9 (Roche). After 24 hours ...
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bioRxiv - Genomics 2020Quote: ... Cell suspension was then supplemented with 1 mM Tris (2-carboxyethyl) phosphine (TCEP) and 1× complete inhibitor cocktail (Roche). Cells were lysed by sonication on ice and then centrifuged at 80,000 g at 4 °C for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Every 10 million cells were lysed by 300μL 1% SDS include freshly made Protease Inhibitor (PI) cocktail (Roche #4693132001), sonicated with Diagenode Bioruptor Pico and diluted with 2.7mL PBA (1x PBS + 0.5% BSA ...
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bioRxiv - Cell Biology 2019Quote: ... and packaging plasmids PEx-QV and pMD-G were co-transfected into HEK 293T cells using Fugene 6 (Roche). After 4 days ...
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bioRxiv - Microbiology 2021Quote: ... and human acute promyelocytic leukemia cell line NB4 (ATCC) were washed once with the digestion buffer for neuraminidase (Roche), the cells were centrifuged at 2000 rpm for 5 minutes ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed in hypotonic buffer (20 mM HEPES pH=8.0, 1x cOmplete EDTA-free protease inhibitor cocktail (Roche) and sonicated three times for 2 minutes ...
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bioRxiv - Cancer Biology 2021Quote: Apoptotic cells were detected using the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL assay; Roche Applied Sciences) according to the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized from 400 ng (melanoblasts) or 1µg (cell lines and tissues) input RNA using Transcriptor first-strand cDNA synthesis kit (04897030001, Roche) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were lysed with 400 μL of NAD+/NADH extraction buffer containing a cocktail of protease inhibitor (Roche Diagnostic ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed in RIPA buffer or high-salt RIPA buffer supplemented with cOmplete EDTA-Free protease inhibitor (Roche) and PhosSTOP phosphatase inhibitor (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... total RNA was extracted from LOUCY cells using the High Pure RNA Isolation Kit (Roche Life Sciences, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... 12 μg of the above vector with 6 μg pCMV8.9 and pCMV-VSVG packaging vectors were transfected into the 293 packaging cell line using X-tremeGENE 9 (Roche), and the resulting viral supernatants were harvested as previously described13 ...
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bioRxiv - Cell Biology 2021Quote: ... NPCs were washed by phosphate buffer saline (PBS) and stained by in situ Cell Death Detection Kit (Roche, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Pelleted cells were resuspended in 1x PBS buffer (pH 7.4) with 5 mM BME and proteinase inhibitor cocktail (Roche). For purification of PABPC1-His ...
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bioRxiv - Cell Biology 2021Quote: ... and thiamine-dependent repression was validated by immuno blotting of whole cell extracts using anti-HA antibodies (Roche, 3F10).
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bioRxiv - Microbiology 2020Quote: ... After 24 h cells were lysed in lysis buffer (0.5 (v/v) % NP-40 in PBS supplemented with protease inhibitors (Roche) and phosphatase inhibitors (Roche) ...
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bioRxiv - Microbiology 2021Quote: Cells were transfected with mammalian construct DNA according to manufacturer instructions with X-tremeGENE 9 DNA Transfection Reagent (Roche).
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bioRxiv - Cell Biology 2020Quote: ... The cells were lysed in immunoprecipitation buffer (50mM Tris–HCl, 0.5% NP-40 PBS with protease inhibitor cocktail (Roche)) 24hr after transfection and subjected to GFP-trap (ChromoTek ...
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bioRxiv - Biophysics 2020Quote: ... a 500 mL cell pellet of Sf9 cells was thawed in 50 mL lysis buffer (25 mM HEPES pH 7.4, 1 M KCl, 10% glycerol, Roche protease inhibitor ...
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bioRxiv - Cancer Biology 2021Quote: Cell pellets and tumor-bearing mammary glands were homogenized in 1% SDS solution containing protease (cat# 11836153001, Roche AG) and phosphatase inhibitor cocktail (cat# 04906845001 ...
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bioRxiv - Cell Biology 2020Quote: ... cell nuclei were counterstained with 100 ng/ml of DAPI for 10 min at room temperature (Roche Applied Science). Slides were mounted on coverslips using Fluoromount mounting medium (DAKO) ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were removed from neurons cultured in MatTek dishes at 8-10 days in vitro (DIV) by incubating cells with extraction buffer (1M sucrose, 0.15% Triton X-100 (Roche, Basel ...
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bioRxiv - Cell Biology 2021Quote: ... The pelleted cells were homogenized in 1X RIPA lysis buffer containing freshly added phosphatase and protease inhibitor cocktail (Roche). To remove cellular debris ...
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bioRxiv - Cell Biology 2022Quote: ... The labeled cells were directly harvested in 1ml of RIPA buffer including 1X EDTA-free protease inhibitor cocktail (Roche). The lysate was cleared by centrifugation at 20,000g for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed in lysis buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 1% NP-40, 2x Roche EDTA-free protease inhibitors ...
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bioRxiv - Cell Biology 2022Quote: ... A single-cell solution was obtained by tissue digestion using 1mgml−1 collagenase D and 0.1mgml−1 DNase I (Roche) in HBSS (Lonza ...
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bioRxiv - Immunology 2022Quote: ... The cells were lysed in TBS/1% (v/v) IGEPAL-630 (sigma) containing 1X Complete protease cocktail inhibitors (Roche). The TCR-CD3 complex was purified from the lysate using Dynabeads M-280 streptavidin (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Transfected Expi293F cell pellets were lysed in PBS + 1% Triton-X100 and Complete protease inhibitor tablets (Roche, Mannheim, Germany) for 15 minutes on ice ...
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were then collected in RIPA buffer containing Complete Mini protease inhibitor cocktail (Roche Diagnostic Systems, Laval, Canada) and lysed through five cycles of freeze-thaw ...
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bioRxiv - Cancer Biology 2022Quote: ... DF1 cells were transfected with the indicated RCAS plasmid using X-tremeGENE 9 DNA transfection reagent (Roche, Catalog# 06365809001) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 x 105 DAOY wild type or 2.5 x 105 HD-MBO3 cells were lysed using RIPA buffer supplemented with protease (Complete Mini, Roche) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified cell lysate was bound to 10 mL of cOmplete His-Tag purification resin (Roche, Germany, 30 min), washed with 1 L base buffer ...
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bioRxiv - Neuroscience 2020Quote: Lysates for whole cell protein analysis were obtained by resuspending cells with Tris-buffered Saline (TBS) supplemented with 0.05% NP40 with the addition of phosphatase and protease inhibitors (Roche) on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... The TUNEL reaction was performed in a humid chamber for 2.5 h at 37 °C with the in Situ Cell Death Detection Kit (Roche) using 100 μL of reaction mixture per slide ...
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bioRxiv - Immunology 2021Quote: ... For experiments in which islets were analyzed using flow cytometry single cell suspensions were generated by digestion with 0.4 Wunsch Units/ml Collagenase D (Roche) and 250 µg/ml DNAse I (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were then lysed in RIPA buffer supplemented with a Complete EDTA-free protease inhibitor cocktail tablet (Roche) and Halt protease ...
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bioRxiv - Microbiology 2021Quote: ... cytotoxicity was determined by lactate dehydrogenase measurements on cell-free supernatants using the Cytotoxicity Detection Kit Plus assay (Roche) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Protein was purified first by resuspending cell pellets in lysis buffer (50 mM Tris-HCl, 300 mM NaCl, pH 8.0, and protease inhibitor tablets (Roche)) and lysed by pulse sonication on ice for 5 minutes in three cycles of 1 s on and 1 s off ...
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bioRxiv - Microbiology 2022Quote: ... 1.5 million S2 cells were transfected with 2 μg of plasmid using Xtreme-GENE HP DNA transfection reagent (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Apoptosis in the migrating primordium was identified using terminal transferase-mediated dUTP nick end-labeling (TUNEL) assay according to manufacturer’s instruction with minor modifications (In situ Cell Death Detection Kit, TMR Red, Roche). Embryos at 30-42 hpf were dechorionated and fixed in 4% PFA overnight at 4°C ...