Labshake search
Citations for Roche :
2251 - 2300 of 3023 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... Vector DNA was transiently transfected into human embryonic kidney (HEK) cells using Fugene-6 (Roche Molecular Biochemicals) transfection reagent ...
-
bioRxiv - Immunology 2021Quote: ... plasma and LN cells were isolated in PBS containing a protease inhibitor cocktail (Roche Diagnostics, Chicago IL) and Ccl19/Ccl21 levels were determined by indirect sandwich of enzyme-linked immunosorbent assay (ELISA ...
-
bioRxiv - Cancer Biology 2020Quote: Cell pellets were homogenized in RIPA buffer (Pierce, Rockford, IL) with complete protease inhibitor (Roche, Basel, Switzerland). Insoluble material was removed by centrifugation at 16,000g at 4oC for 15mins ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were stained with Bromodeoxyuridine (BrdU) and assessed with a BrdU ELISA kit (Roche, Mississauga, ON, Canada) following the manufacturer’s protocol.
-
bioRxiv - Developmental Biology 2021Quote: ... Cell suspensions were prepared by digesting the tissue in a cocktail consisting of Liberase TL (Roche, 5401020001) and DNase I (Sigma ...
-
bioRxiv - Immunology 2020Quote: ... blood and BM cells for both species) with a LightCycler® 480 Real-Time PCR System (Roche). Gene expression was then assessed with the BioMark HD (Fluidigm ...
-
bioRxiv - Immunology 2020Quote: ... cells were washed with PBS and lysed in RIPA buffer with added protease and phosphatase inhibitors (Roche). Samples were sonicated ...
-
bioRxiv - Immunology 2021Quote: ... cells were lysed with RIPA lysis buffer containing complete protease inhibitor cocktail and phosphatase inhibitor cocktail (Roche) for the detection of S protein by western blot or ELISA ...
-
bioRxiv - Genomics 2021Quote: Mice livers were perfused and dissociated into single cells using Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) as previously described8 ...
-
bioRxiv - Neuroscience 2021Quote: ... and cells were lysed in RIPA buffer supplemented with 1x cOmplete protease inhibitor cocktail (Roche, Basel, Switzerland). 20 μl of each sample was analysed in duplicates using the BD™ CBA Human Soluble Protein Master Kit (#556264 ...
-
bioRxiv - Neuroscience 2021Quote: ... cells or dentate gyrus were lysed and sonicated in RIPA lysis buffer (iNtRON) with proteinase inhibitor (Roche), followed by incubation with streptavidine-agarose beads (Pierce ...
-
bioRxiv - Immunology 2022Quote: Cell pellets were lysed in cold RIPA buffer containing a cocktail of protease inhibitors (Roche, Cat# 11697498001). Quantification of proteins was conducted via the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2022Quote: ... Glucose was measured in cell medium using the Roche diagnostic kit on a Cobas 8000 (Roche Diagnostics) as described previously ...
-
bioRxiv - Cancer Biology 2022Quote: Cells were lysed with RIPA buffer (Wako Chemicals, 182-02451) containing cOmplete protease inhibitor cocktail (Roche, 05056489001) and PhosSTOP phosphatase inhibitor cocktail (Roche ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cell pellets were resuspended in PBS buffer supplemented with 5 mM imidazole and protease inhibitors (cOmplete, Roche). Cells were lysed by sonication and incubated at 80°C in a water bath for 10 min with sporadic manual agitation ...
-
Synthetic lethal targeting of TET2-mutant hematopoietic stem and progenitor cells by XPO1 inhibitorsbioRxiv - Cancer Biology 2022Quote: ... TUNEL apoptosis assay was performed on embryos with the In-Situ Cell Death Detection kit (POD: Roche) according to the manufacturer’s recommendations at 37°C for 5 hours ...
-
bioRxiv - Cancer Biology 2022Quote: ... drug-treated cells were lysed in RIPA buffer supplemented with a phosphatase inhibitor cocktail (Roche, Mannheim, Germany) and 1mM PMSF on ice for 20min ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were pre-incubated with 20 mU ml- 1 Vibrio cholerae-derived neuraminidase (sialidase) (#11080725001, Roche, Switzerland) for 1 h at 37°C on a cell shaker ...
-
bioRxiv - Cell Biology 2022Quote: ... cell pellets were lysed in IgG Buffer supplemented with cOmplete™ EDTA-free protease inhibitor cocktail (Roche) and 0.2 mM Mg-ATP ...
-
bioRxiv - Molecular Biology 2022Quote: ... Supernatants was aspirated and cells were lysed in 2 PCVs Buffer A containing 1x CPI (Roche, #11836145001), 0.5 mM DTT and 0.15 % NP40 ...
-
bioRxiv - Microbiology 2022Quote: ... and lentivirus vectors at a 2:8:10 μg ratio to HEK293T cells using Xtreme-Gene9 (Roche) and lentivirus-containing supernatants were collected 72hr post-transfection ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... blastocytes were subject to the TUNEL reaction using the In-Situ Cell Death Detection Kit (Roche, 11684809910) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: TUNEL assay was carried out following the manufacturer’s instructions (In Situ Cell Death Detection Kit, Roche-11684795910).
-
bioRxiv - Neuroscience 2022Quote: ... Transient transfection was achieved by transfecting cells with plasmid DNAs using XtremeGene HP DNA Transfection reagent (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: Cell lysates were prepared in radioimmunoprecipitation assay buffer supplemented with protease inhibitor cocktail tablets (Roche, Basel, Switzerland), and total protein was measured using a Bicinchoninic Acid Protein Assay Kit (Beyotime Biotechnology ...
-
bioRxiv - Cancer Biology 2022Quote: Cells were harvested and lysed using the RIPA lysis buffer supplemented with protease inhibitor cocktail (Roche, 04693124001). The frozen tumor tissues were homogenized with RIPA lysis buffer (with protease inhibitor ...
-
bioRxiv - Immunology 2023Quote: Cell pellet or tissue were lysed in RIPA lysis buffer containing 1× proteinase inhibitor cocktail (Roche, 5056489001), 2 mM PMSF and 1 mM DTT ...
-
Reduction of Spermine Synthase Suppresses Tau Accumulation Through Autophagy Modulation in TauopathybioRxiv - Neuroscience 2023Quote: ... On the fifth day, harvest the cells with RIPA buffer (R0278, Thermo) with proteinase inhibitors (11836170001, Roche) and phosphatase inhibitors (04906837001 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... fixed cells were treated with 333 µL PBS supplemented with 1 µL RNAse (10 mg/mL) (Roche) at 37°C for 30 min ...
-
bioRxiv - Cell Biology 2023Quote: ... and the cell pellet was resuspended in 150 μl of the hypotonic Cell Fraction Buffer (10 mM HEPES, 1.5 mM MgCl2,10 mM KCl, pH 7.9 plus PIM (Roche)) ...
-
bioRxiv - Microbiology 2023Quote: ... and the cell suspension were digested in HBSS containg 25 μg /ml of DNAse I (#11284932001, Roche) and 50 μg/ml Liberase (#5401119001 ...
-
bioRxiv - Cell Biology 2023Quote: ... gRNA constructs were transfected into HeLa S3 cells (https://www.atcc.org/products/ccl-2.2) with X-tremeGENE 9 (Roche) overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells (1×105) were then trypsinized and plated on collagen pre-treated cover glasses (Roche Applied Sciences) followed by treatment with GO or Bleo ...
-
bioRxiv - Molecular Biology 2023Quote: ... the LDH present in cell-free supernatants was detected in triplicates using the Cytotoxicity Detection kit (Roche) according to manufacturer instructions ...
-
bioRxiv - Cell Biology 2023Quote: SA2 cDNAs were cloned directly from HCT116 cells by PCR using KAPA HiFi HotStart PCR kit (Roche) (Fwd ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were lysed with 1 ml lysis buffer (0.5 % Nonident-P40, 2 % protease inhibitor cocktail (Roche, Switzerland) and 1 % phosphatase inhibitor cocktails I and II (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2023Quote: The viable cell density (VCD) and viability were measured using a Cedex HiRes Analyzer (Roche, Basel, Switzerland) from daily samples ...
-
bioRxiv - Biophysics 2023Quote: ... and harvested cell pellets resuspended in 250 μl lysis buffer containing a complete protease inhibitor cocktail (Roche). After centrifugation (15,000 × g for 5 min at 4°C) ...
-
bioRxiv - Genomics 2023Quote: Cell-free fDNA and gDNA libraries were constructed using the Kapa Hyper Prep kit (cat: 07962363001, Roche) with custom dual index duplex UMI adapters (IDT) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were lysed by scrapping in RIPA buffer supplemented with EDTA-free protease inhibitors (Roche, cat. # 04693159001). Lysed cells were centrifuged at maximal speed for 10 min ...
-
bioRxiv - Cell Biology 2023Quote: ... gRNA constructs were then sequence-verified and transfected into penta KO cells with X-tremeGENE 9 (Roche) overnight and single cells that were positive for GFP were sorted by fluorescence activated cell sorting (FACS ...
-
bioRxiv - Molecular Biology 2023Quote: ... 200 µL of cell culture supernatant was collected and the Cytotoxicity Detection Kit (LDH) (Roche, Indianapolis, IN) was then utilized to detect LDH levels in the culture supernatant ...
-
bioRxiv - Microbiology 2022Quote: Antigen retrieval was conducted using a Tris based buffer-Cell Conditioning 1 (CC1)-Catalog # 950-124 (Roche). The SARS-CoV-2 spike primary antibody was of rabbit origin ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell-free supernatants were collected and assessed via LDH release assay with a Cytotoxicity Detection Kit (Roche). The percent LDH release was calculated according to the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2023Quote: Resuspended cells were homogenized by gentle stirring and supplemented with an EDTA-free protease inhibitor cocktail (Roche). The cell suspension was passed three times through an M-110 PS microfluidizer (Microfluidics ...
-
bioRxiv - Biochemistry 2023Quote: Resuspended cells were homogenized by gentle stirring and supplemented with an EDTA-free protease inhibitor cocktail (Roche). The cell suspension was passed three times through an M-110 PS microfluidizer (Microfluidics ...
-
bioRxiv - Neuroscience 2023Quote: ... in a PBS-based cell lysis buffer complete with EDTA-free protease inhibitors (#04693159001; Roche, Mississauga, Canada) and 1 mmol/L phenylmethylsulfonyl fluoride (#PMSF123.5 ...
-
bioRxiv - Immunology 2023Quote: ... tissue was finely minced and cell dissociation was performed using a mixture of liberase TH (Roche, 05401135001) and DNase-I (Roche ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed in RIPA buffer supplemented with protease and phosphatase inhibitors (mini cOmplete and phosSTOP, Roche) on ice for 10 min.
-
bioRxiv - Molecular Biology 2023Quote: Cell lysates were prepared in 1x PXL lysis buffer supplemented with complete protease inhibitors (Roche, cat. #11873580001). All lysates were prepared from equal cell numbers per condition ...