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Citations for Roche :
2151 - 2200 of 3023 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... treated cells were lysed in 0.1% Triton X-100 with phosphate and protease inhibitors (Roche, Inc.) and the resulting pellet after high-speed centrifugation was then dissolved in 1% Triton X-100 ...
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bioRxiv - Neuroscience 2022Quote: Cell death was determined by lactate dehydrogenase (LDH) activity using a Cytotoxicity Detection Kit (LDH) (Roche). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were lysed on ice in phosphate buffered saline (PBS) containing phosphatase (PhosphoSTOP Tablet, Roche) and protease (Complete Mini Tablets ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with In Situ Cell Death Detection (TUNEL) Kit with TMR Red (Roche, 12156792910) according to the vendor’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were incubated with two lysis buffers containing a complete protease inhibitor cocktail (Roche, 11697498001) (Paro Rinse 1 ...
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bioRxiv - Cell Biology 2023Quote: ... Infected cells were cultivated in the presence of IL-2 (10 IU/mL, Roche, Basel, Switzerland). Three days after coinfection cells were sorted based on expression of mouse CD48 and mCD24 (BD biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were scraped into ice-cold PBS supplemented with protease inhibitors (cOmplete protease inhibitor cocktail, Roche) and phosphatase inhibitors ...
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bioRxiv - Biophysics 2023Quote: ... Transfected cells were grown to mid log (OD = 0.7) and induced with 0.4 mM IPTG (Roche) and grown overnight at 16°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were lysed in 150 µL RIPA buffer with 2x complete protease inhibitor cocktail (Roche) on ice for 10 minutes ...
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bioRxiv - Biochemistry 2023Quote: Tissue homogenates or cell lysates were prepared in RIPA lysis buffer supplemented with protease inhibitors (Roche) and subjected to Western blotting as previously described30 ...
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bioRxiv - Immunology 2023Quote: ... Adherent cells were lysed in 50 uL of RIPA buffer supplemented with protease inhibitor cocktail (Roche). Cell lysate and cell supernatant were homogenized by pipetting up and down ten times and supplemented with laemli buffer (1X final ...
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bioRxiv - Cell Biology 2023Quote: Lipofection: Plasmid was lipofected into cells using X-tremeGENE 9 DNA Transfection Reagent (Roche; Basel, Switzerland). The plasmid:transfection reagent ratio (w:v ...
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bioRxiv - Immunology 2023Quote: The cell lysis buffer 10 M urea containing the cOmplete™ protease inhibitor cocktail (Roche, #11697498001) was used for protein extraction ...
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bioRxiv - Cancer Biology 2022Quote: Total cell lysate was obtained by lysing in 1x RIPA buffer supplemented with protease inhibitors (Roche) and MG132 (Calbiochem) ...
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bioRxiv - Genetics 2022Quote: ... Total RNA was extracted from ES cells using the High Pure RNA Isolation Kit (Roche Diagnostics) and RTPCR performed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... Pathology-grade, pre-diluted PAX8 (Cell Marque, no RRID available) and WT1 (Roche, no RRID available) antibodies were detected with the Ultraview DAB detection kit on the Ventana Benchmark Ultra.
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bioRxiv - Cell Biology 2023Quote: Cells were lysed on ice for 10 min in RIPA buffer containing protease inhibitors (Roche, complete) and phosphatase inhibitors ...
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bioRxiv - Immunology 2022Quote: Cells were lysed using RIPA buffer (Pierce) supplemented with Complete Mini EDTA-free Protease Inhibitor (Roche). Lysate protein concentration was determined using BCA protein assay kit (Pierce) ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were cultured in RPMI + IL-2 (final concentration 100 U/mL, Roche, 10799068001), IL-7 (final conc ...
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bioRxiv - Cancer Biology 2023Quote: Du145 (5 × 103) and 22Rv1 (1 × 104) cells were seeded on 96-well E-Plates (Roche). Proliferation was monitored every 1 h and time dependent cell index (CI ...
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bioRxiv - Bioengineering 2023Quote: ... Transfections of HEK293T and 293FT cells were performed using X-tremeGENE 9 DNA Transfection Reagent (Roche). Murine Stem Cell Viruses (MSCV ...
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bioRxiv - Neuroscience 2023Quote: ... The brain slices were processed using the In Situ Cell Death Detection Kits (Roche Life Science) according to manufacturer’s instruction.
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bioRxiv - Microbiology 2023Quote: ... Cells were washed once with PBS and resuspended in Lysis buffer (PBS + Protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2023Quote: Cells for immunoblots were lysed in ice cold RIPA buffer with protease and phosphatase inhibitors (Roche). Lysates were quantified using a DC protein assay (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 2.5 ug plasmid DNA per well using DOTAP Liposomal Transfection Reagent (Roche) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... cells were harvested by centrifugation and lysed by sonication in native buffer including Complete Mini (Roche) and Lysozyme (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... mechanically dissociated in a single cell suspension in media containing DNase I (Roche, 100 ug/ml) using repeated pipetting and concentrated by centrifugation (3 min at 800 X g) ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed with PBS and subsequently lysed in RIPA buffer containing protease inhibitor cocktail (Roche). Protein samples were resolved by SDS–PAGE on 4-12% Nu-Page gels (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with DAPI (1/2000 in sterile PBS from 1 mg/ml stock, Roche). Sorted cells were re-suspended in medium to reach a 2.5 million cells/ml concentration ...
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bioRxiv - Cancer Biology 2024Quote: ... were transfected into the human 293FT cell line using X-tremeGENE 9 DNA transfection reagent (Roche). After 48 hours ...
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bioRxiv - Microbiology 2024Quote: ... plasmids were transfected into HeLa cells using X-tremeGENE HP DNA transfection reagent (Roche, Laval, QC) according to manufacturer’s protocols.
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bioRxiv - Bioengineering 2024Quote: ... cells were harvested from their matrices using liberase DL research grade (24 U; Roche, Basel, Switzerland) and stained with the following antibodies (diluted 1:500 in PBS ...
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bioRxiv - Biophysics 2024Quote: ... 5% glycerol) per 1 L of cells with one EDTA-free protease inhibitor cocktail tablet (Roche). Cells were lysed using a Misonix Sonicator 3000 (110 W for 2 min total ON-time ...
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bioRxiv - Cell Biology 2024Quote: ... D-Mel (d.mel-2) cells were transfected using X-tremeGene HP DNA transfection reagent (#06366236001, Roche). Two days after transfection ...
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bioRxiv - Cell Biology 2024Quote: RNA extraction from cultured cells has been performed using the High Pure RNA Isolation Kit (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was purified from one million cells with the High Pure RNA Isolation kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and pLentiCRISPRv2-PFN1sgRNA (500 ng each) into HEK293FT cells using X-tremeGENE HP transfection reagent (Roche). Lentivirus was harvested 72 hours later and subsequently used to infect JR20 cells supplemented with 4 μg/mL of Polybrene ...
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bioRxiv - Molecular Biology 2020Quote: Proliferative capacity of cells was analysed using 5-bromo-2’-deoxy-uridine (BrdU) labelling (Roche, Basel, Switzerland). Cells seeded on coverslips were pulsed with 10 μM BrdU for 60 min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Whole cell extracts were prepared by lysis in RIPA buffer supplemented with protease and phosphatase inhibitors (Roche). Protein concentration was quantified by BCA assay (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed with lysis buffer (500 μL) containing the cOmplete protease inhibitor cocktail (Roche, cat#11697498001). The biotinylated surface proteins were excluded with NeutrAvidin agarose gel (Pierce ...
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bioRxiv - Cell Biology 2020Quote: ... at pH 7.4] and seeded at a density of 50–75 × 103 cells/plate on laminin (Roche) coated plates ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were harvested on ice in Pierce IP lysis buffer and EDTA-free protease inhibitor cocktail (Roche), sonicated ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed 3x with ice cold PBS and lysed with lysis buffer with protease inhibitors (Roche). Cell lysate was centrifuged for 30 min at 15000 g and supernatant was applied to streptavidin-conjugated magnetic beads (Dynabeads® MyOne™ Streptavidin C1 ...
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bioRxiv - Cell Biology 2020Quote: Mammalian cells were lysed in Radioimmunoprecipitation assay buffer (RIPA) buffer with the addition of protease (Roche cOmplete), and phosphatase inhibitors (GB Sciences) ...
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bioRxiv - Microbiology 2020Quote: ... The DNA fragmentation assay used the Cell Death Detection ELISA kit (initially from Roche, later Millipore-Sigma). Phosphatidylserine was detected by FITC-conjugated annexin V (Sigma) ...
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bioRxiv - Immunology 2021Quote: Macrophage cell lysates were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitor cocktails and PhosSTOP (Roche). Protein concentration was measured using the BCA Protein Assay Kit (Pierce) ...
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bioRxiv - Immunology 2021Quote: The cell pellets were lysed in 8M urea/50mM Tris pH 8.1/100mM NaCl + protease inhibitors (Roche) and quantified by BCA assay (Pierce) ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed in IP Lysis Buffer (87787, Pierce™) supplemented with protease inhibitors (Complete mini, Roche), and IP was performed with antibodies against survivin (RnD AF886 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... by using In Situ Cell Death Detection Kit (TMR red cat no. 1684795, Roche, Indianapolis, IN, USA) according to manufacturer’s recommendation ...
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bioRxiv - Immunology 2022Quote: ... and pLenti-Luc-GFP (6 µg) into HEK293T cells using X tremeGENE HP DNA Transfection Reagent (Roche) according to the manufacturer′s instructions ...