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Citations for Roche :
2201 - 2250 of 3023 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... TUNEL staining was performed according to manufacturer’s instructions (In situ cell death detection kit TMR red, Roche). For the positive control ...
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bioRxiv - Developmental Biology 2019Quote: TUNEL was performed as described in [68] using In Situ Cell Death Detection Kit provided by Roche Diagnostics ...
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bioRxiv - Microbiology 2019Quote: ... cells were resuspended in buffer B1 supplemented with complete EDTA-free protease inhibitor cocktail (Roche, Basel, Switzerland), 1 mg/ml lysozyme ...
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bioRxiv - Microbiology 2019Quote: ... a first stage consisted in lysing the cell pellets by beat beating with MagNA Lyser Instrument (Roche Diagnostics ...
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bioRxiv - Microbiology 2019Quote: ... Cells were subsequently solubilised in 1% Triton X-100 in PBS containing 1x cOmplete protease inhibitor (Roche) and incubated on ice for 20 min ...
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bioRxiv - Microbiology 2019Quote: ... Infected red blood cells were lysed on ice with chilled hypotonic buffer (1 mM HEPES/NaOH, Roche cOmplete EDTA-free protease inhibitor ...
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bioRxiv - Cancer Biology 2019Quote: ... Cytofluorimetric recordings of phosphatidylserine exposure on the cell surface (increased staining of FITC-conjugated Annexin-V; Roche) and loss of plasma membrane integrity (7-AAD staining ...
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bioRxiv - Systems Biology 2019Quote: ... The Th17 cultured cells were stimulated in presence of IL-6 (20ng/ml, Roche, Cat# 11138600 001); IL-1β (10ng/ml ...
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bioRxiv - Biochemistry 2021Quote: ... coli cell lysate (in Buffer C supplemented with a cocktail of protease inhibitors (cOmplete, EDTA-free (Roche)) ...
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bioRxiv - Immunology 2021Quote: Mouse lung cells were obtained by digestion of lung tissue with 0.05 mg/ml Liberase TL (Roche) and 0.5 mg/ml DNaseI (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Cells were resuspended in AYE media containing a complete mini EDTA-free protease inhibitor cocktail tablet (Roche) and adjusted to an OD600 of 20 ...
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bioRxiv - Microbiology 2021Quote: Cells were washed in PBS and lysed in NTEN buffer supplemented with phosphatase and protease inhibitors (Roche). Protein concentrations were determined using a BCA assay (Pierce) ...
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bioRxiv - Bioengineering 2021Quote: Cell membrane integrity was measured via the lactate dehydrogenase activity (LDH) release assay (Roche Cytotoxicity Detection Kit). Media from the bottom channel was continuously collected in 15 minutes intervals (both for the unstretched and stretched mimicking VILI) ...
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bioRxiv - Neuroscience 2021Quote: ... for 5 minutes and cells were washed in cold PBS supplied with protease inhibitor cocktail (11873580001, Roche). Cells were stored as dry cell pellet at -80°C until further processed ...
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bioRxiv - Neuroscience 2020Quote: ... The cell suspension was filtered through a 70 µm filter with DNAse (66 U/mL) (Roche Diagnostics). Cell pellet was resuspended in 25% density gradient and centrifuged at 521 g for 20 min at 18° C ...
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bioRxiv - Cell Biology 2021Quote: Cells were re-suspended in lysis buffer containing 2x concentration of PIC (Roche complete, EDTA-free #05056489001) and 2 mM PMSF ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mM β-mercaptoethanol and supplemented with complete EDTA-free cocktail tablets (1 tablet/50ml cells; Roche), 0.01 mg/ml DNase (Sigma ...
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bioRxiv - Systems Biology 2020Quote: ... The cell pellets were then lysed by adding 10 M urea containing complete protease inhibitor cocktail (Roche) and Halt™ Phosphatase Inhibitor (Thermo) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell pellets were lysed in 250 μl IP Lysis Buffer (Pierce) supplemented with complete protease inhibitors (Roche) for 30 minutes at 4 °C ...
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bioRxiv - Microbiology 2021Quote: Culture density was measured with a haemocytometer (BSFs) or a CASY model TT cell counter (Roche Diagnostics) with a 60 µm capillary and exclusion of particles with a pseudo diameter below 2.0 µm (PCFs).
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bioRxiv - Microbiology 2020Quote: ... GISAID: EPI_ISL_413570) obtained from cell culture using the MagNA Pure LC Total Nucleic Acid Isolation Kit (Roche). We determined the slope by linear regression in GraphPad Prism and defined the required levels for PCR efficiency (E ...
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bioRxiv - Cell Biology 2021Quote: ... Assay was performed according to protocol in Cell proliferation kit II (XTT) (Roche, #11 465 015 001).
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bioRxiv - Biochemistry 2022Quote: ... Cells were transfected with a total of 1 µg of DNA using Fugene 6 (Roche, Branchburg, NJ) according to the manufacturer’s instructions
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bioRxiv - Molecular Biology 2022Quote: ... the cell culture suspension was supplemented with DNase I and EDTA-free protease inhibitor cocktail tablet (Roche) and lysed by passing through an EmulsiFlex-05 cell disruptor (Avestin ...
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bioRxiv - Molecular Biology 2022Quote: ... the cell culture suspension was supplemented with DNase I and EDTA-free protease inhibitor cocktail tablet (Roche) and cells were lysed by passing through an EmulsiFlex-05 cell disruptor (Avestin ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-cell library quantification is completed using a Roche LightCycle96 using FastStart Essential Green Master (Roche 06402712001) and KAPA Library Quant (Illumina ...
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bioRxiv - Genetics 2022Quote: Frozen mouse tissues were homogenized in CelLytic M cell lysis reagent (MilliporeSigma) and cOmplete protease inhibitors (Roche) was added to prevent protein degradation ...
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bioRxiv - Cancer Biology 2022Quote: ... DF-1 cells were transfected with the relevant RCAS viral plasmids using Extreme-Gene 9Transfection reagent (Roche) accordingly to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed with 2% paraformaldehyde/ 2% sucrose for 20 min and stained with DAPI (#10236276001, Roche) to visualize anaphases ...
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bioRxiv - Microbiology 2020Quote: A colorimetric assay based on the measurement of LDH activity released by damaged cells was used (Roche). Experiments were performed in 96-well plate and the manufacturer’s instructions were followed ...
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bioRxiv - Microbiology 2021Quote: ... medium was removed and cells were lysed with MagNA Pure 96 external lysis buffer (Roche, Penzberg, Germany). Isolation of RNA from cell lysates and quantitative RT-PCR on subgenomic nucleocapsid RNA was performed as described elsewhere (Corman et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... All cell lysis buffers were supplemented with fresh EDTA-free complete protease inhibitor cocktail (CPI, Roche #11836153001). Purified chromatin was sonicated with a Bioruptor (Diagenode ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were resuspended in 200 µL PBS containing 1× Complete EDTA-free protease inhibitor cocktail (Roche) and sonicated by Bioruptor II (BMBio) ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was isolated from 2.5×107 cells using the High Pure PCR Template Preparation kit (Roche). 1.1 µg DNA was then either treated with 10 U recombinant E ...
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bioRxiv - Immunology 2020Quote: Skin samples from mice were enzymatically digested to release cells using 1 mg/ml collagenase D (Roche), 0.5 mg/ml dispase (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... All the cells in culture were negative on periodically testing for mycoplasma using Mycoplasma Detection Kit (Roche). All the human and mouse cells ...
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bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in complete media plus 10 IU/mL recombinant human IL-2 (rHIL-2, Roche), and seeded in fresh media at 0.5×106 cells/mL every 48 hours ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa WT cells were transfected with the guide containing plasmid using the transfection reagent XtremeGENE HP (Roche) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Barrier function was assessed using the xCELLigence Real-Time Cell Analyzer (RTCA, Acea Biosciences/Roche Applied Science) to measure electrical impedance across HUVEC monolayers seeded onto microelectrodes ...
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bioRxiv - Systems Biology 2020Quote: ... The cell pellets were immediately lysed by adding 10 M urea containing complete protease inhibitor cocktail (Roche) and Halt™ Phosphatase Inhibitor (Thermo ...
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bioRxiv - Cancer Biology 2020Quote: ... cell pellet was resuspended in lysis RIPA buffer supplemented with protease inhibitor cocktail and phosphatase inhibitors (Roche) and incubated in ice for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated with 1:500 mouse anti-actin26 and 1:1000 rat anti-HA (3F10, Roche) followed by 1:1000 anti-mouse Alexa 488 (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... The cell-free DNA library was prepared using the KAPA Library Preparation Kit (Kapa Biosystems, Wilmington, USA). The gDNA library was constructed according to our established method [15] ...
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bioRxiv - Pathology 2020Quote: ... cells were dissolved in RIPA buffer containing a protease inhibitor cocktail (Roche Applied Science, Indianapolis, IS, USA) were quantitated using the BCA protein assay kit (Energenesis- Biomedical ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were lysed on ice with RIPA buffer containing protease and phosphatase inhibitors (Complete and PhosSTOP, Roche). Samples were centrifuged for 3 minutes at 15000xg to remove cell debris and treated with DNAse to degrade genomic DNA ...
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bioRxiv - Systems Biology 2019Quote: ... The cells were then mechanically disrupted with 0.1 mm Zirconia beads in a MagNA Lyser instrument (Roche) by agitating the samples 4 times at 7000 rpm for 45 seconds with a cooling step at −20°C for 5 minutes between each cycle ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cell viability was measured by trypan blue exclusion or WST-1 assays (Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Immunology 2020Quote: ... 1.2×106 cells were lysed in 300 μL RIPA buffer supplemented with cOmplete Protease Inhibitor Cocktail (Roche) on ice for 30 minutes and passed through 30 μm polyethylene filter spin columns (Pierce ...
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bioRxiv - Cell Biology 2020Quote: Cells were harvested in RIPA Lysis Buffer (strong) (CWbio, Taizhou, China; CW2333S) containing protease inhibitors (Roche, 4693124001). A total of 40 µg proteins were separated by 4-20% gradient SDS-PAGE gels (GenScript ...
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bioRxiv - Biochemistry 2021Quote: ... the cell culture suspension was supplemented with DNase I and EDTA-free protease inhibitor cocktail tablet (Roche) and cells were lysed by passing through an EmulsiFlex-05 cell disruptor (Avestin ...