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Citations for Roche :
2101 - 2150 of 3023 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... cells were washed once with PBS before 350 µl of external lysis buffer (Roche, Penzberg, Germany) was added to the cells ...
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bioRxiv - Molecular Biology 2020Quote: ... cell pellets were resuspended in 750μL of ice-cold 0.05% Nonidet P-40 (Roche Diagnostics, Germany) in PBS and 200μL of the lysate was removed as “whole cell lysate” ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were lysed with the Lysis Binding Buffer from the Magnapure LC Kit 03038505001 (Roche) containing > 3 M guanidine thiocyanate (GTC) ...
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bioRxiv - Microbiology 2021Quote: ... The cDNAs were transfected into cells using the X-tremeGENE HP DNA transfection reagent (Roche, 06366546001). Then the transfected cells were infected by concentrated SARS-CoV-2 pseudotyped virus 48 h later ...
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bioRxiv - Neuroscience 2020Quote: ... The cell lysates (1 μg/μL) were enzymatically digested with 50 μg/mL pronase E (Roche) at 37°C for 1 hour followed quenching with protease inhibitors and SDS-PAGE loading buffer ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were transfected with Lpd-specific or scramble shRNA as control using Fugene HD (Roche) transfection reagent ...
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bioRxiv - Immunology 2021Quote: ... Th17-cell differentiation was induced using a cytokine cocktail of IL-6 (20 ng/mL; Roche), IL-1β (10 ng/mL ...
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bioRxiv - Immunology 2019Quote: For the in vitro cell stimulation and maintenance reagents were as follows: human IL-2 (Roche); IL-21 (PeproTech) ...
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bioRxiv - Cell Biology 2022Quote: Cells were lysed on ice in RIPA buffer supplemented with Protease Inhibitor Cocktail (Cat#. 11873580001, Roche). Lysates were cleared by centrifuging at 15,000g and 4°C for 20min (Sorvall Legend Micro 17R Centrifuge ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was extracted from cell lines using the High Pure RNA Isolation Kit (Roche, Basel). 1 μg of extracted RNA was reverse transcribed into cDNA using the Transcriptor First Strand Synthesis kit (Roche) ...
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bioRxiv - Plant Biology 2019Quote: ... according to the manufacture’s protocol of the in situ Cell Death Detection kit (Roche Diagnostics, Germany). Imaging was done with Nikon ECLIPSE 80i fluorescence microscope (Nikon ...
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bioRxiv - Cancer Biology 2019Quote: ... Total cell lysate was harvested with RIPA buffer containing protease inhibitors (protease inhibitor cocktail tablets, Roche) on the third day ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were washed twice with PBS and lysed with RIPA buffer containing protease inhibitor cocktail (Roche). Protein quantification was performed using the method of the Pierce™ BCA protein assay kit according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then collected and lysed in the presence of a protease inhibitor cocktail (Roche) and phosphatase inhibitors ...
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bioRxiv - Cancer Biology 2020Quote: ... Cellular extracts were prepared by incubating the cells in lysis buffer containing protease inhibitor cocktail (Roche). Anti-FLAG immunoaffinity columns were prepared using anti-FLAG M2 affinity gel (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The cell proliferation was continuously monitored by using the xCELLigence RTCA Instrument (xCELLigence RTCA, Roche, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Single cell suspensions were then prepared by incubating minced tissue in 1 mg/ml collagenase (Roche) and 50 U/ml DNase I (Roche ...
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bioRxiv - Immunology 2020Quote: ... -C and -G in a pX330 vector was transfected into HAP1 cells using X-tremeGENE (Roche). Single cells were FACS sorted based on W6/32 negativity to obtain knockout clones for HLA-A ...
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bioRxiv - Developmental Biology 2020Quote: ... was performed with the In Situ Cell Death Detection Kit/Fluorescein (Roche, 11 684 795 910). Slides were first pretreated with a 2:1 mix of Ethanol:Acetic Acid 5min -20C ...
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bioRxiv - Molecular Biology 2021Quote: ... Apoptosis was analyzed using TUNEL assay by an In Situ Cell Death Detection kit (11684795910, Roche). As counterstain for IF and IHC stainings Hoechst or DAPI staining was used ...
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bioRxiv - Microbiology 2019Quote: ... the cells were broken using an Emulsilfex C3 (Avestin) in presence of proteases inhibitor (Complete, Roche). The lysate was used to bind to 200ul of IgG Sepharose beads (Amersham Biosciences ...
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bioRxiv - Immunology 2019Quote: ... Cellular content of the preparations was assessed by cell counting using a Casey TT counter (Roche) in combination with multi-colour flow-cytometry.
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bioRxiv - Cell Biology 2021Quote: Apoptosis in kidneys was detected using the In Situ Cell Death Detection Kit (Sigma Aldrich/Roche). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were lysed in RIPA Lysis Buffer (CWBIO, CW2333S) containing EDTA-free cOmplete(Roche, 4693132001) with sonication on ice ...
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bioRxiv - Bioengineering 2021Quote: HEK293T cells were lysed in RIPA lysis buffer (Applygen) supplemented with complete protease inhibitor cocktail (Roche) 48 h after transfection ...
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bioRxiv - Pathology 2021Quote: ... Cell apoptosis was examined utilizing a TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) kit (Roche). In brief ...
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bioRxiv - Biophysics 2021Quote: Cells were harvested and lysed in RIPA lysis buffer containing a protease inhibitor mix (Roche #4693159001). Lysates were centrifuged at 10,000 x g for 10 min and 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... whole cell lysate of primary myoblasts was prepared by lysing buffer containing protease inhibitor cocktail (Roche). Protein concentration was determined with the BCA Protein Assay Reagent (Pierce Biotechnology ...
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bioRxiv - Biophysics 2021Quote: ... Monoclonal cell lines were identified by immunocytochemistry using a rat anti-HA antibody (Roche Applied Science). HEK293 cells stably overexpressing rat sACt were as described (58).
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bioRxiv - Immunology 2021Quote: ... Th17 cell polarization was induced using a cytokine cocktail of IL-6 (20 ng/mL; Roche), IL-1β (10 ng/mL ...
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bioRxiv - Genetics 2020Quote: ... Cells were permeabilized with 0.02% digitonin in the Wash Buffer along with protease inhibitors (Roche # 04693159001) for 5 mins at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... assays were carried out using the in Situ Cell Death Detection Kit (11684795910; Roche, Basel, Switzerland) as previously described (Liu et al. ...
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bioRxiv - Genetics 2019Quote: ... Cells were thawed in 30 mL lysis buffer containing protease inhibitors (Complete EDTA-free, Roche #11836170001), lysozyme and benzonase (Sigma-Aldrich #E8263) ...
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bioRxiv - Genetics 2019Quote: ... Cells were scraped in PBS supplemented with protease inhibitor cocktail (1:100, 11836145001, Roche applied science) and phosphatase inhibitor cocktail (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: H1975 cells were lysed in RIPA lysis assay buffer (Pierce) supplemented with enzyme inhibitor cocktail (Roche). The samples were separated on a 4–20% SDS-polyacrylamide gel (Biorad) ...
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bioRxiv - Cell Biology 2022Quote: ... The construct was then sequenced and transfected into penta KO cells with X-tremeGENE 9 (Roche) overnight and GFP positive single cells were sorted by fluorescence activated cell sorting (FACS ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were collected 48 hours post-transfection and sonicated in PBS with protease inhibitor mixture (Roche). Samples were processed and analyzed by western blot as described above.
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bioRxiv - Microbiology 2022Quote: ... Cell-clone colonies were tested for β-galactosidase expression to check viral infectivity (kit Roche #11758241001). Positive clones were transduced with the lentiviral vector (LV ...
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bioRxiv - Biochemistry 2022Quote: ... cell pellets were thawed and mixed with two cOmplete ETDA-free protease inhibitor cocktail tablets (Roche) and 50 μL of benzonase (Molecular Biology
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bioRxiv - Biochemistry 2022Quote: DNA from liver and cell pellets was isolated using High Pure PCR Template Preparation Kit (Roche). DNA concentrations were measured by Nanodrop 2000c Spectrophotometer (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed in lysis buffer supplemented with 1x phosphatase inhibitors (PhosStop, Roche Diagnostics, Basel, Switzerland) and a 1x protease inhibitor cocktail (Complete Mini ...
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bioRxiv - Cell Biology 2022Quote: Transient DNA transfection of these cells was performed using X-tremeGENE HP DNA Transfection Reagent (Roche) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed with the radioimmune precipitation assay buffer containing a cocktail of protease inhibitors (Roche). After removal of the cell debris ...
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bioRxiv - Genetics 2022Quote: Cells were washed in PBS and resuspended in RIPA buffer (CST#9806) containing proteinase inhibitors (Roche) and quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... BLP20 and BLP21 respectively and transduced cell populations were selected with 1mg/ml of G418 (Roche).
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bioRxiv - Microbiology 2022Quote: ... A549 cells with different Sia abundance were prepared by treatment with different concentrations of CpNA (Roche) for 30 min at 37 °C ...
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bioRxiv - Pathology 2022Quote: Tissue and cell lysates were produced in RIPA buffer supplemented with PhosSTOP (Roche Diagnostics, Indianapolis, IN) and protease inhibitor cocktail (Roche Diagnostics) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then lysed in RIPA buffer with 1× EDTA-free protease inhibitor cocktail (Roche) and analyzed using western blots.
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bioRxiv - Neuroscience 2023Quote: To detect DNA fragmentation in GFAP+ astrocytes an in situ cell death detection kit (Roche, 11684795910) was used ...
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bioRxiv - Neuroscience 2023Quote: Cells were lysed in non-denaturing lysis buffer supplemented with EDTA-free protease inhibitor cocktail (Roche) on ice ...