Labshake search
Citations for Roche :
2401 - 2450 of 3182 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: Mice or Human CD4 T cells were lysed using RIPA buffer supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitors (Pierce) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell extracts were obtained by washing 3 × in PBS and either lysing for RNA (TriPure reagent, Roche) or protein extraction (50 mM Tris-HCl ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proliferation rate in vitro was assessed using an xCELLigence Real-Time Cell Analyzer (RTCA) system (Roche Diagnostics)49 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cell lines were transfected with siRNAs using xTremeGene siRNA transfection reagent (1ul/well, Roche, #4476093001; standard protocol). After addition of siRNA transfection mix ...
-
bioRxiv - Immunology 2024Quote: Antigen retrieval was conducted using a Tris based buffer-Cell Conditioning 1 (CC1)-Catalog # 950-124 (Roche). The MHCII primary was of mouse origin ...
-
bioRxiv - Cell Biology 2023Quote: HeLa cells were lysed with lysis buffer (0.5% Triton X-100 in PBS) containing protease inhibitors (Roche) for 15 min on ice ...
-
bioRxiv - Cell Biology 2024Quote: Cells were lysed in reducing Laemmli SDS sample buffer containing PhosSTOP (Phosphatase Inhibitor Cocktail Tablets, Roche, Switzerland) at 96°C for 10 min and the proteins separated on NuPAGE® Novex® 4–12% Bis–Tris Gels ...
-
bioRxiv - Immunology 2023Quote: Cell pellet or tissue were lysed in RIPA lysis buffer containing 1× proteinase inhibitor cocktail (Roche, 5056489001), 2 mM PMSF and 1 mM DTT ...
-
Reduction of Spermine Synthase Suppresses Tau Accumulation Through Autophagy Modulation in TauopathybioRxiv - Neuroscience 2023Quote: ... On the fifth day, harvest the cells with RIPA buffer (R0278, Thermo) with proteinase inhibitors (11836170001, Roche) and phosphatase inhibitors (04906837001 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... fixed cells were treated with 333 µL PBS supplemented with 1 µL RNAse (10 mg/mL) (Roche) at 37°C for 30 min ...
-
bioRxiv - Cell Biology 2023Quote: ... and the cell pellet was resuspended in 150 μl of the hypotonic Cell Fraction Buffer (10 mM HEPES, 1.5 mM MgCl2,10 mM KCl, pH 7.9 plus PIM (Roche)) ...
-
bioRxiv - Microbiology 2023Quote: ... and the cell suspension were digested in HBSS containg 25 μg /ml of DNAse I (#11284932001, Roche) and 50 μg/ml Liberase (#5401119001 ...
-
bioRxiv - Cell Biology 2023Quote: ... gRNA constructs were transfected into HeLa S3 cells (https://www.atcc.org/products/ccl-2.2) with X-tremeGENE 9 (Roche) overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells (1×105) were then trypsinized and plated on collagen pre-treated cover glasses (Roche Applied Sciences) followed by treatment with GO or Bleo ...
-
bioRxiv - Molecular Biology 2023Quote: ... the LDH present in cell-free supernatants was detected in triplicates using the Cytotoxicity Detection kit (Roche) according to manufacturer instructions ...
-
bioRxiv - Cell Biology 2023Quote: SA2 cDNAs were cloned directly from HCT116 cells by PCR using KAPA HiFi HotStart PCR kit (Roche) (Fwd ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were lysed with 1 ml lysis buffer (0.5 % Nonident-P40, 2 % protease inhibitor cocktail (Roche, Switzerland) and 1 % phosphatase inhibitor cocktails I and II (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2023Quote: The viable cell density (VCD) and viability were measured using a Cedex HiRes Analyzer (Roche, Basel, Switzerland) from daily samples ...
-
bioRxiv - Biophysics 2023Quote: ... and harvested cell pellets resuspended in 250 μl lysis buffer containing a complete protease inhibitor cocktail (Roche). After centrifugation (15,000 × g for 5 min at 4°C) ...
-
bioRxiv - Genomics 2023Quote: Cell-free fDNA and gDNA libraries were constructed using the Kapa Hyper Prep kit (cat: 07962363001, Roche) with custom dual index duplex UMI adapters (IDT) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were lysed by scrapping in RIPA buffer supplemented with EDTA-free protease inhibitors (Roche, cat. # 04693159001). Lysed cells were centrifuged at maximal speed for 10 min ...
-
bioRxiv - Cell Biology 2023Quote: ... gRNA constructs were then sequence-verified and transfected into penta KO cells with X-tremeGENE 9 (Roche) overnight and single cells that were positive for GFP were sorted by fluorescence activated cell sorting (FACS ...
-
bioRxiv - Molecular Biology 2023Quote: ... 200 µL of cell culture supernatant was collected and the Cytotoxicity Detection Kit (LDH) (Roche, Indianapolis, IN) was then utilized to detect LDH levels in the culture supernatant ...
-
bioRxiv - Microbiology 2022Quote: Antigen retrieval was conducted using a Tris based buffer-Cell Conditioning 1 (CC1)-Catalog # 950-124 (Roche). The SARS-CoV-2 spike primary antibody was of rabbit origin ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell-free supernatants were collected and assessed via LDH release assay with a Cytotoxicity Detection Kit (Roche). The percent LDH release was calculated according to the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2023Quote: Resuspended cells were homogenized by gentle stirring and supplemented with an EDTA-free protease inhibitor cocktail (Roche). The cell suspension was passed three times through an M-110 PS microfluidizer (Microfluidics ...
-
bioRxiv - Biochemistry 2023Quote: Resuspended cells were homogenized by gentle stirring and supplemented with an EDTA-free protease inhibitor cocktail (Roche). The cell suspension was passed three times through an M-110 PS microfluidizer (Microfluidics ...
-
bioRxiv - Neuroscience 2023Quote: ... in a PBS-based cell lysis buffer complete with EDTA-free protease inhibitors (#04693159001; Roche, Mississauga, Canada) and 1 mmol/L phenylmethylsulfonyl fluoride (#PMSF123.5 ...
-
bioRxiv - Immunology 2023Quote: ... tissue was finely minced and cell dissociation was performed using a mixture of liberase TH (Roche, 05401135001) and DNase-I (Roche ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed in RIPA buffer supplemented with protease and phosphatase inhibitors (mini cOmplete and phosSTOP, Roche) on ice for 10 min.
-
bioRxiv - Molecular Biology 2023Quote: Cell lysates were prepared in 1x PXL lysis buffer supplemented with complete protease inhibitors (Roche, cat. #11873580001). All lysates were prepared from equal cell numbers per condition ...
-
bioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in sodium phosphate buffer (PBS) with a Complete Protease Inhibitor Cocktail Tablet (Roche) and lysed using French press at 10,000 psi ...
-
bioRxiv - Cancer Biology 2023Quote: The cell pellets originating from primary hepatocytes were lysed in cold HEN Buffer supplemented with PhosSTOP (Roche) and cOmplete™ ...
-
bioRxiv - Cell Biology 2023Quote: ... washed with PBS and permeabilised at 1 × 107 cells/ml in MELB with cOmplete protease inhibitors (Roche) and 0.025% digitonin (w/v ...
-
bioRxiv - Cell Biology 2023Quote: ... Plasmids were transiently transfected in HeLa cells for 48 hours using X-tremeGENE 9 DNA reagent (Roche). For silencing experiments ...
-
bioRxiv - Microbiology 2023Quote: Cell lysates were prepared in lysis buffer containing 1% Nonidet P-40 and protease inhibitor cocktail (Roche) (Cao ...
-
bioRxiv - Immunology 2023Quote: Cells were lysed with RIPA complete lysis buffer supplemented with EDTA-free protease and phosphatase inhibitors (Roche) and 25 U of Benzonase Nuclease ...
-
bioRxiv - Immunology 2023Quote: ... Cells were lysed with lysis buffer (500 μL) containing the complete protease inhibitor cocktail (Roche, cat#11697498001). The biotinylated surface proteins were excluded with NeutrAvidin agarose gel (Pierce ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were then washed twice more in PBS and stained with 1 μg/mL DAPI (Roche, 10236276001) in PBS for 5 min at room temperature ...
-
bioRxiv - Microbiology 2024Quote: ... Genomic DNA was purified from 106 infected cells using the High pure PCR template preparation kit (Roche) and subjected to quantitative real-time PCR (qPCR) ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were incubated for 30 min at 37 °C with mouse anti-BrdU (11296736, Roche. 1/10) then washed before incubation at room temperature for 3 h with the primary antibody anti-myosin heavy chain (MF20 ...
-
bioRxiv - Molecular Biology 2024Quote: ... EBs were dissociated into single cells by treatment with 1 mg/ml Dispase II (Roche, 04942 078001) and 2.5 mg/ml collagenase I (Gibco ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were then transfected with the respective plasmids using X- tremeGENE™ HP DNA transfection reagent (Roche, #6365779001) for 48 h (24 h for Toca-1 and Cdc42).
-
bioRxiv - Cell Biology 2020Quote: ... the cells were incubated with the anti-HA high affinity antibody (3F10) (1:500 dilution, Roche – cat. 11867423001), followed by incubation with an anti-rat secondary antibody conjugated to Alexa Fluor 488 (1:2000 dilution ...
-
bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from 3 million HEK293T cells or 0.75 million primary neurons using TriPure reagent (Roche) and purified using RNeasy MinElute Cleanup Kit (Promega ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were lysed using ice-cold Farnham lab buffer supplemented with complete protease inhibitor cocktail (#4693159001, Roche, Switzerland). Chromatin was sheared with Bioruptor (Diagenode ...
-
bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from asynchronous RPE-1 cells using the High Pure RNA Isolation kit (Roche, 11828665001) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were then lysed using 0.4 mL lysis buffer containing 2x protease inhibitors (Complete, Mini, EDTA-free; Roche), 1x phosphatase inhibitors (PhosStop ...
-
bioRxiv - Immunology 2021Quote: The presence of lactate dehydrogenase (LDH) in cell culture supernatants was measured using the Cytotoxicity Detection kit (Roche), following manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: ... Harvested cells in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS ...