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Citations for Roche :
2051 - 2100 of 3023 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were then washed in 10 mL staining media containing DNase I (Roche, Basel, Switzerland) and then resuspended in 100 μL of staining media containing 4 μg/mL of biotinylated RAVV GPΔMuc conjugated to streptavidin PE and 4 μg/mL of biotinylated RAVV GPΔMuc conjugated to streptavidin APC and incubated for 20 minutes [45] ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated in complete RPMI-10 supplemented with 50U/mL hIL-2 (Roche 11011456001), 2.5ng/mL TGFβ (R&D 240-B-10) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed in radioimmunoprecipitation assay buffer containing complete protease inhibitor cocktail (Roche, Basel, Switzerland) on ice and ultracentrifuged ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of the 10x single cell library was accurately determined through qPCR (Kapa Biosystems) to produce cluster counts appropriate for the HiSeq 4000 or NovaSeq 6000 platform (Illumina) ...
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bioRxiv - Microbiology 2024Quote: ... Sonicated cells were then digested with 6μL RNaseA (SIG 10109169001, Sigma-Aldrich/Roche, 10mg/mL), 5.4 μL of 100mM MnCl2 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were further lysed by sonication in the presence of protease inhibitor cocktail (Roche, USA). Protein concentration from cell extracts was measured using Bio-rad Protein Assay kit ...
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bioRxiv - Developmental Biology 2024Quote: ... Apoptosis staining was done using TMR red In Situ Cell Death Detection Kit (121567910, Roche). Brain sections (5 um ...
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bioRxiv - Molecular Biology 2024Quote: Transfection was performed on HEK293T cells using the X-tremeGENE HP DNA Transfection Reagent (Roche) following manufacturer’s manual and viral supernatant was collected 48 hours after transfection ...
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bioRxiv - Microbiology 2024Quote: ... The cells were lysed by adding Lysozyme (MP-Biomedicals) and DNase I (Roche Applied Sciences) and incubating them on ice for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed with ice-cold PBS containing protease and phosphatase inhibitor cocktails (Roche, Germany), scraped and pelleted by centrifugation.
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bioRxiv - Cancer Biology 2024Quote: Lenti-vectors were prepared in 293T cells using X-tremeGene HP DNA transfection reagent (Roche), with psPAX2 and pMD2.G (Addgene ...
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bioRxiv - Cell Biology 2024Quote: Cells were collected in RIPA buffer containing 1x cOmplete EDTA-free protease inhibitor (Roche, 05056489001) and were then mechanically lysed by passage through a 27G needle on ice ...
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bioRxiv - Microbiology 2024Quote: ... total RNA from fractionated cells was isolated with a High Pure RNA Isolation kit (Roche). (i ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed in ice-cold FACS buffer (2% FBS, (0.5 ug/ml) DNase I (Roche), 2 mM EDTA in PBS) ...
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bioRxiv - Immunology 2021Quote: Splenocytes lysates were prepared using Cell Lysis Buffer (#9803, CellSignaling Technology) supplemented with protease inhibitors (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: N2a cells were lysed in RIPA buffer (Beyotime, P0013B) supplemented with 1x protease inhibitor cocktail (Roche). Total cell lysates were separated on 8-12% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... After dissection cell suspensions were prepared by vortexed with 1.5μl 1x Liberase TM (Roche, LIBTM-RO) in 500 μl cold PBS ...
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bioRxiv - Neuroscience 2021Quote: Cell lysates in 1% sarcosyl were digested with 75 µg/ml proteinase K (PK; Roche, Switzerland) for 1 h at 37 °C and subsequently denatured in an equal volume of 2x sample loading buffer at 110 °C ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed three times with 1X PBS supplemented with protease inhibitor cocktail without EDTA (Roche) and solubilized in Native PAGE sample buffer supplemented with 10% glycerol ...
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bioRxiv - Immunology 2021Quote: ... Viral plasmids were transduced in HEK-293T cells using X-tremeGENE HP DNA transfection reagent (Roche). After the addition of X-tremeGENE HP to the viral plasmids the solution was mixed in 100 µL of DMEM medium without serum or antibiotics ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were collected by scraping in PBS supplemented with the EDTA-free protease inhibitor tablets (Roche) and centrifuged 10 min at 2000 rpm and 4° C.
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bioRxiv - Genetics 2019Quote: ... Cells were harvested and disrupted by sonication in cold PBS buffer containing protease inhibitor cocktail (Roche), 10 mM β-mercaptoethanol ...
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bioRxiv - Neuroscience 2020Quote: ... Treated or untreated transfected HEK cells were lysed in cold IP buffer with protease inhibitors (Roche), and centrifuged for 10 minutes and 5,000 rpm at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and a single-cell suspension was prepared by digesting the tissue in collagenase type A (Roche) and 25 μg/ml DNase (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed twice in ice-cold phosphate buffered saline containing protease inhibitor cocktail tablets (Roche). Cells were scraped into conical tubes ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lysed using radioimmunoprecipitation assay (RIPA) buffer supplemented with a cocktail of protease inhibitors (Roche). Western blot analysis was performed using standard procedures ...
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bioRxiv - Cell Biology 2021Quote: ... CFBE41o- and 16HBE14o- cells was isolated using a High Pure RNA Isolation Kit (Roche, Mannheim, Germany) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: HEK293T cells were lysed in RIPA lysis buffer (Applygen) supplemented with complete protease inhibitor cocktail (Roche) 48 h after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... after translations the SP cells were incubated on ice with 10 µg/ml proteinase K (Roche) in the presence of 1 mM CaCl2 for 25 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested and resuspended in 2 ml of 1X PBS containing protease inhibitor cocktail (Roche) and 500μl of resuspended cells was taken as a control for uncrosslinked sample (referred to as -DSG in the text) ...
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bioRxiv - Microbiology 2020Quote: ... Cells separated from supernatants were lysed in RIPA buffer containing freshly added protease inhibitors cocktail (Roche), for 30 min on ice ...
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bioRxiv - Microbiology 2020Quote: ... cells were resuspended in 1 ml staining buffer containing 0.1 mg/ml RNaseA (Roche Life Sciences) and incubated at room temperature (RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed in lysis buffer supplemented with 1x phosphatase inhibitors (PhosStop, Roche Diagnostics, Basel, Switzerland) and a 1x protease inhibitor cocktail (Complete Mini ...
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bioRxiv - Cancer Biology 2020Quote: ... Target cells with PBMCs in complete medium or supplemented with 5% Triton X-100 (Roche Diagnostics) were used to determine minimum and maximum release ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in RIPA buffer supplemented with Complete Phosphatase and Complete Protease inhibitor cocktails (Roche). After dilution in Laemmli buffer with DTT and boiled for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were arrested at metaphase by incubating with colcemid (N-methyl-N-deacetyl-colchicine, Roche, 10295892001) for the last 14 hours before harvesting the cells ...
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bioRxiv - Cell Biology 2021Quote: Fluorescent TUNEL staining was performed using the Fluorescein In Situ Cell Death Detection Kit (11684795910, Roche), with the fluorescein detected by antibody staining using rabbit anti-FITC ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates (0.5 mg) were incubated with anti-HA antibody-coupled agarose beads (Roche, Basel, Switzerland) for 16 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... TUNEL staining was performed according to manufacturer’s guideline (In-Situ Cell Death Detection Kit, Fluorescein, Roche). All staining was performed on 3 hearts per group ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was extracted from 106 cells using High Pure PCR Template Preparation Kit (Roche, 11796828001). 150 ng of purified DNA was digested in Reaction Buffer (200 mM sodium acetate [pH 4.5] ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were reverse transfected with 1:3 or 1:6 X-tremeGENE 9™ (Roche) transfection reagent according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... The cells were directly harvested in RIPA buffer including 1X EDTA free protease inhibitor cocktail (Roche) and cleared by centrifugation at 15,000g for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells were suspended in RIPA lysis buffer (T&I) containing a protease inhibitor cocktail (Roche). After centrifugation ...
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bioRxiv - Immunology 2022Quote: ... Cells of the LP were isolated from fibrous matter using collagenase IV (680 UI/ml, Roche) and DNAse I (1 mg/ml ...
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bioRxiv - Immunology 2022Quote: ... the cells were the lyzed in digest buffer with proteinase inhibitor cocktail and phosSTOP (Roche, Switzerland) on ice ...
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bioRxiv - Biochemistry 2022Quote: ... Cell membranes were solubilized in the lysis buffer plus 1% LMNG and Protease Inhibitor Cocktail (Roche) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified cell lysates were incubated with 10 mL of cOmplete His-Tag purification resin (Roche, Germany) for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were transfected with these mixtures using 2.4μL of X-tremeGENE HP DNA Transfection Reagent (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets were resuspended in 100 or 250 μL Permeabilization Buffer containing protease (Roche, cat# 11836170001) and phosphatase (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... The cell pellet was resuspended in CER I buffer supplemented with phosphatase and protease inhibitors (Roche) to isolate cytosolic fraction and lysed using CER II buffer ...