Labshake search
Citations for Roche :
2001 - 2050 of 3023 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... HEK293T and CHO-K1 cells were transfected with the FuGENE6 transfection reagent (Roche Applied Bioscience) and selected by the addition of puromycin (1 μg/ml and 10 μg/ml respectively ...
-
bioRxiv - Cell Biology 2021Quote: ... plasmids were introduced into HEK293T cells using the FuGENE 6 transfection reagent (Roche Applied Science). Cells were selected with puromycin (1 μg/ml) ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were then permeabilized in 1 % bovine serum albumin (BSA; Roche Diagnostics GmbH, Germany, 10735078001) in PBS-Triton 0.1 % solution for 5 min and blocked in 1 % BSA in PBS at room temperature for 1 h ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were lysed at 3 days in ice-cold RIPA buffer containing protease inhibitors (Roche) and protein concentration was determined by BCA Assay (Gibco BRL ...
-
bioRxiv - Microbiology 2021Quote: ... cells were labeled beforehand for 30 minutes with an anti HA antibody (3F10, Roche 11867423001) diluted at 1:200 in PBA ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer containing cOmplete EDTA-free protease inhibitor (Roche) and 10U/ml benzonase nuclease (Santa Cruz) ...
-
bioRxiv - Microbiology 2021Quote: ... cells were lysed in NP-40 lysis buffer containing 1× cOmplete Protease Inhibitor Cocktail (Roche) and 1× Phenylmethylsulfonyl fluoride (Sigma Aldrich ...
-
bioRxiv - Cancer Biology 2021Quote: Cells were lysed in ice-cold RIPA buffer containing a Complete protease inhibitor tablet (Roche). Protein concentration was measured using the Pierce BCA Protein Assay Kit (Life Technologies) ...
-
bioRxiv - Neuroscience 2021Quote: Cell pellets were lysed in ice-cold RIPA buffer supplemented with c0mplete protease inhibitors (Roche). Whole-cell lysates were then sonicated and clarified by centrifugation at 14,000xg for 15 minutes at 4°C ...
-
bioRxiv - Microbiology 2022Quote: Whole-cell lysates were prepared using Triton-X sample buffer containing protease inhibitor cocktail (Roche). The protein concentration was assessed by using DC Protein assay reagent (Bio-Rad Laboratories) ...
-
bioRxiv - Cell Biology 2022Quote: ... anti-Myc (WB, 1:2000; 9E10, Cat. #11667149001, Roche; labeled as IR for 293FT cells). Generation and validation of rabbit polyclonal antibodies against GFP was described previously(65 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Frozen cell pellets were lysed in PBS supplemented with Benzonase (Santacruz) and protease inhibitors (Roche) using a chilled bath sonicator (QSONICA ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were collected and lysed using 300 μL of TNI buffer with protease inhibitor (Roche) sonicated at room temperature for 10 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... cells were incubated in a 1:1000 dilution of 1 mg/ml DAPI (Roche Diagnostics) in PBS for 5 min followed by three washes with PBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5×106 K562 cells were resuspended in PBS with EDTA-free protease inhibitor cocktail (Roche) and lysed using a 27.5G needle ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were then resuspended in 1mL PBS with 1× EDTA-free protease inhibitor cocktail (Roche) from which 5 μl of the cells were lysed in 50 μl of 1% Triton lysis buffer to obtain whole cell protein levels ...
-
bioRxiv - Immunology 2022Quote: ... Cells were digested in RPMI-1640 media containing 20 μg/mL DNase (Roche, Basel, Switzerland) and 1 mg/mL collagenase IV (StemCell Technologies ...
-
bioRxiv - Cell Biology 2022Quote: ... The concentrations of LDH released from cells were determined with a Cytotoxicity Detection Kit (Roche), as described previously 46.
-
bioRxiv - Physiology 2022Quote: ... Antigen retrieval for p21 was performed with Cell Conditioning 1 (CC1) buffer (Roche, 950-124). Secondary antibodies used were the OmniMap anti-Rat HRP (Roche ...
-
bioRxiv - Biochemistry 2022Quote: Cells were lysed in 40 μl RIPA buffer supplemented with 1 × cOmplete protease inhibitors (Roche) and the soluble protein recovered by centrifugation (40 min at 21k × g) ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with Fugene using standard procedures and subsequently selected using Hygromycin (Roche, 10843555001). U2OS cell line expressing H2B-mNeon/mCherry-CENP-A and HeLaEGFP-AID-Sororin cell line expressing H2B-MNeon were obtained by lentiviral transduction (plasmids pLV-H2B-Neon-ires-Puro58 and pLenti6-CENP-A-mCherry59 ...
-
bioRxiv - Microbiology 2022Quote: Whole-cell lysates were prepared using Triton-X sample buffer containing protease inhibitor cocktail (Roche). The protein concentration was assessed by using DC Protein assay reagent (Bio-Rad Laboratories) ...
-
bioRxiv - Pathology 2023Quote: TUNEL staining was performed using the In Situ Cell Death detection kit (Roche Diagnostic, USA). Briefly ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell suspensions were quickly washed with 150 µg/mL trypsin inhibitor (Roche, Basel, Switzerland, 10109878001) and 666.67 µg/mL DNase 1 (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were lysed in the presence of a protease inhibitor cocktail (Roche cOmplete EDTA-free) in 1 mL of an ice-cold solubilization buffer (SB ...
-
bioRxiv - Cell Biology 2023Quote: ... Transfection of HEK-293 cells was performed with FuGene 6 transfection reagent (Roche, Mannheim, Germany) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... Both plasmids were transfected into HeLa cells using the X-tremeGENE9 DNA Transfection Reagent (Roche) and selected in medium containing G418 (1 mg/mL) ...
-
bioRxiv - Neuroscience 2023Quote: ... TUNEL assay was performed using the In-Situ Cell Death Detection Kit (TMR Red) (Roche) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... RNA from dermal fibroblasts cell pellets were extracted using High Pure RNA Isolation Kit (Roche) using manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Nup96-GFP KI U2OS cells were transfected using X-tremeGENETM 9 DNA Transfection Reagent (Roche), and 24h after transfection ...
-
bioRxiv - Pathology 2023Quote: ... TUNEL staining was performed using TMR-Red in situ cell death detection kit (Roche, 12156792910) per the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... BrdU incorporation was carried out according to the manufacturer′s specifications (Cell Proliferation ELISA, Roche) with an incubation time of 5 hours as previously described.23 Quantification was performed by measuring the absorbance using Power Wave 340 ELISA reader from Bio-Tek.
-
bioRxiv - Cancer Biology 2023Quote: ... Addgene #12259 into HEK-293T cells using X-tremeGENE HP DNA transfection reagent (#06366236001; Roche). Lentiviral supernatants were applied to cells for 48h in the presence of polybrene (10 µg/ml ...
-
bioRxiv - Cancer Biology 2023Quote: ... The TUNEL assay was performed using the In Situ Cell Death Detection Kit (Roche Diagnostics) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in Buf1*(10 ml Buf.1 + protease inhibitor cocktail (Roche, Basel, Switzerland) and lysed with MULTI BEADS SHOCKER (YASUI KIKAI ...
-
bioRxiv - Microbiology 2023Quote: ... The supernatant was removed and 1 ml of red blood cell lysis buffer (Roche 11814389001) was added and cells were incubated for 1 minute ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were stained with a 1 µg/ml solution of the DNA-dye DAPI (Roche) and mounted with Slowfade antifade reagent (Invitrogen) ...
-
bioRxiv - Bioengineering 2023Quote: ... High Pure RNA Isolation Kit and Cell Proliferation Reagent WST-1 were purchased from Roche. QIAshredder and RNeasy Fibrous Tissue Mini Kit were purchased from Qiagen ...
-
bioRxiv - Bioengineering 2023Quote: ... The cells were washed twice with PBS and stained with DAPI (0.5 µg/mL) (Roche) for 10 min at RT ...
-
bioRxiv - Biochemistry 2023Quote: DNA synthesis or replication was monitored by measuring the Cell Proliferation ELISA BrdU kit (Roche). Cells were cultured on a Nunc 96-well plate (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... The concentration of the 10x single-cell library was accurately determined through qPCR (Kapa Biosystems) to produce cluster counts appropriate for the HiSeq4000 or NovaSeq6000 platform (Illumina) ...
-
bioRxiv - Molecular Biology 2023Quote: TUNEL analysis was performed using In Situ “Cell Death Detection Kit” (TMR Red) (Roche, #12156792910).
-
bioRxiv - Microbiology 2023Quote: ... cells were lysed in 1% Triton X-100 buffer with phosphatase and protease inhibitors (Roche). Protein was separated by 4-12% Bis-Tris protein gel (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... C2C12 cell lysates were prepared in RIPA buffer containing protease inhibitors (Roche Diagnostics, Indianapolis, IN). To examine mitochondrial-associated proteins ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were collected by scraping in 1mL PBS containing Complete EDTA-free Protease Inhibitor (Roche) and pelleted ...
-
bioRxiv - Developmental Biology 2023Quote: ... TUNEL staining was performed using the In Situ Cell Death Detection Kit (Roche, Cat #11684817910) according to the manufacturer’s specifications ...
-
bioRxiv - Biophysics 2023Quote: ... Cell Proliferation Reagent WST-1 and Cytotoxicity Detection KitPLUS (LDH) were obtained from Roche (Germany). 5× HOT FIREPol® EvaGreen® qPCR Supermix was acquired from Solis BioDyne (Estonia) ...
-
bioRxiv - Cell Biology 2023Quote: ... The plasmids were transiently transfected into HeLa cells using Fugene HD transfection reagent (Roche, Germany) for 48 hours to express the corresponding E2F-EGFP fusion proteins ...
-
bioRxiv - Cell Biology 2023Quote: ... The cell pellets were resuspended in 1x TNE containing EDTA-free Protease inhibitor cocktail (Roche). Following protein estimation using the Bradford method ...
-
bioRxiv - Cell Biology 2023Quote: ... The cell pellet was washed with PBS supplemented with protease inhibitors (mini cOmplete cocktail, Roche) and snap frozen in liquid nitrogen ...