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Citations for Roche :
1951 - 2000 of 7357 citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The cell pellets were mixed with 10-fold volume of sucrose buffer (0.25 M sucrose; protease and phosphatase inhibitor cocktails (Roche)) and homogenized until a completely homogenous solution was obtained ...
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bioRxiv - Physiology 2021Quote: ... Muscles were homogenized in buffer (2% sodium dodecyl sulfate, 50□mM Tris-HCl, pH 6.8) with protease and phosphatase inhibitors (Roche). Protein concentrations were determined by Bicinchoninic acid assay (Sigma-Aldrich ...
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bioRxiv - Physiology 2020Quote: ... were sonicated in lysis buffer (2% SDS, 60 mM Tris-HCl, pH 6.8) with phosphatase and protease inhibitors (Roche). Protein concentration was measured using a BCA assay (Pierce) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4°C in B1 buffer with alkaline phosphatase-conjugated anti-DIG (1/2000; Roche 11633716001). After three washes in B1 buffer and one wash in B3 buffer (100 mM Tris–HCl pH 9 ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were incubated overnight at 4°C with 1:2000 alkaline phosphatase-conjugated anti-DIG antibodies (Sigma/Roche, 11093274910). After washes and prior to development ...
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bioRxiv - Evolutionary Biology 2020Quote: ... tissues were incubated at 4°C in a solution of 0.05% alkaline phosphatase-conjugated DIG antibodies (Roche cat #11093274910) overnight ...
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bioRxiv - Cell Biology 2021Quote: ... 5 ml NP-40 in a total volume of 500 ml ddH2O) containing phosphatase and protease inhibitor cocktail (Roche) then centrifuged for 30 min at 12000 rpm at 4°C ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: Cells were lysed using RIPA lysis and extraction buffer with protease inhibitors and phosphatase inhibitors from Roche (Indianapolis, IN). Protein concentration were determined with the Bio-Rad protein assay (Hercules ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.34 M Sucrose, 10% Glycerol, 1 mM DTT, 0.1% TritonX100 added with 1x PI and 1x Phosphatase Inhibitors - Roche) and left on ice for 8 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were washed twice with ice cold PBS and scraped into cold PBS with phosphatase inhibitors (PhosSTOP, Roche). Pelleted cells were snap-frozen and lysed with 8M urea buffer with 100nM TEAB (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... EDTA-free TCA buffer was used where phosphorylation of proteins was to be investigated by alkaline phosphatase-treatment (Roche) or Phos-tagTM (NARD Institute ...
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Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafishbioRxiv - Developmental Biology 2022Quote: ... The probes labeled by digoxigenin were detected using alkaline phosphatase coupled anti-digoxigenin Fab fragment antibody (Roche, Basel, Switzerland) with 5-bromo-4-chloro-3-indolyl-phosphate nitro blue tetrazolium staining (Vector Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: ... cell pellets were lysed in whole-cell extraction buffer (150 mM NaCl, 50 mM Tris pH 7.5, 1% NP-40) supplemented with proteinase and phosphatase inhibitors (Roche). The lysates were cleared by centrifugation at 14,000 rpm for 30 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... 150 mM NaCl, 50 mM HEPES pH 7.5, 1x EDTA-free protease inhibitor cocktail [Roche], 1x PhosSTOP phosphatase inhibitor cocktail [Roche]). Lysates were clarified by centrifugation at 17,000 x g for 15 min at 4°C ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... 0.5% Triton X-100) supplemented with fresh protease and phosphatase inhibitors (1 mM PMSF, 0.1 mM Na3VO4 and 1x PIC - Roche). Cell lysates were passed through a 1 ml syringe for mechanical disruption and centrifuged for 10 min at 13.000 rpm ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... 0.5% Triton X-100) supplemented with fresh protease and phosphatase inhibitors (1 mM PMSF, 0.1 mM Na3VO4 and 1x PIC - Roche), followed by three washes with kinase buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... and 2 mM Na-EGTA [pH 7.5]) that was supplemented with a cOmplete Protease Inhibitor Cocktail Tablet and Phosphatase Inhibitor Tablet (Roche) and 1 mM phenylmethylsulfonyl fluoride ...
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bioRxiv - Cell Biology 2022Quote: ... which were pre-washed with NP-40 lysis buffer with protease/phosphatase inhibitors (Roche, cat# 11697498001 and cat# 4906845001) and incubated for 2.5 hours at +4C° with gentle agitation ...
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bioRxiv - Cell Biology 2022Quote: ... the water was aspirated and Triton X-100 buffer (Triton X-100, 0.5 M EDTA, PBS, ddH2O) with phosphatase (Roche) and protease (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed in 8 M urea buffer (8 M urea, 1 M Tris pH 7.4, 5 M NaCl) containing protease and phosphatase inhibitors (Roche) followed by sonication at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at 4°C with anti-digoxigenin-alkaline phosphatase antibodies (1:5000; Roche; 11093274910; AB_514497) diluted in blocking solution ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM EDTA, 20 mM HEPES, 0.2% NP-40, 10% glycerol, 400U Ribolock inhibitor, 1x protease/phosphatase inhibitor, Roche). Lysates were centrifuged (12000 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... Detection was performed using alkaline phosphatase-conjugated anti-digoxigenin Fab fragments and the chemiluminescent substrate CSPD (Roche Applied Science). ImageJ analysis was performed to calculate the relative intensity of Maxi-probe detected bands with the corresponding control-probe detected bands.
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bioRxiv - Microbiology 2023Quote: ... titrated to pH 8.1 with phosphoric acid) with protease and phosphatase inhibitors added (Roche, CO-RO and PHOSS-RO) and sonicated ...
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bioRxiv - Microbiology 2023Quote: ... and lysed immediately in ice-cold lysis buffer (20 mM Tris. HCl, pH 8.5, 8M urea, phosphatase inhibitors (PhosSTOP, Roche), 2 mM dithiothreitol (DTT ...
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bioRxiv - Cell Biology 2023Quote: ... insect Sf9 cells were infected with appropriate baculovirus and then lysed in lysis buffer (PBS, pH 7.2, 250 mM NaCl, 30 mM imidazole, and protease and phosphatase inhibitors [Roche]), passing the cell suspension 30 times through a 21- gauge syringe needle ...
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bioRxiv - Cell Biology 2023Quote: ... 500mg of each embryonic cryolysed were resuspended in 1ml of lysis buffer ((Tris 25mM pH7.5, NaCl 100mM, MgCl2 2mM, DTT 1mM, phosphatase inhibitors (PhosSTOP EASYpack, Roche) and protease inhibitors (cOmplete ...
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bioRxiv - Neuroscience 2024Quote: ... Human tissues were homogenized in TBS (20 mM Tris-HCl, 140 mM NaCl, pH 7.5) containing protease and phosphatase inhibitors (Roche). Following homogenization ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole cellular protein was extracted with RIPA buffer (Serva electrophoresis) containing protease inhibitor cocktail and phosphatase inhibitor cocktail (Roche). Western blots were performed following routine protocols55 ...
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bioRxiv - Physiology 2024Quote: ... and 0.005% bromophenol blue) containing phosphatase and protease inhibitors (PhosSTOP and cOmplete ULTRA tablets, mini, EASYpack protease inhibitor cocktail, Roche), and proteins were analyzed by Western blot ...
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bioRxiv - Molecular Biology 2024Quote: Cells were collected 48 hours after transfection in 1xPBS pH 7.4 with Protease and Phosphatase Inhibitor Cocktail (Roche Diagnostics). The lysates were sonicated for 10 seconds at 40–50% power ...
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bioRxiv - Microbiology 2024Quote: ... Schizonts were then lysed in the presence of 0.15 % saponin in PBS containing cOmplete Mini EDTA-free Protease and PhosSTOP Phosphatase inhibitor cocktails (Roche) for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and resuspended in 300 µL cold nuclear lysis buffer (1% SDS, 10 mM EDTA, 50 mM tris-HCl pH8.0, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min on ice) ...
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bioRxiv - Molecular Biology 2024Quote: ... The remaining supernatant was diluted in 1 mL pre-warmed capturing buffer (0.5 M LiCl, 4 M urea, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche) and 10 µL from an equimolar stock (10 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... and resuspended in 50 µL cold nuclear lysis buffer (1% SDS, 10 mM EDTA, 50 mM tris-HCl pH8.0, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 mM EDTA, 20 mM HEPES, 0.2% NP-40, 10% glycerol, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche). Lysates were centrifuged (12000 rpm ...
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bioRxiv - Cell Biology 2024Quote: ... containing 5% beta-mercaptoethanol and protease and phosphatase inhibitors (Roche Complete Mini protease inhibitor, Sigma-Aldrich #11836153001, and Roche Complete Ultra phosphatase inhibitor ...
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bioRxiv - Molecular Biology 2024Quote: ... after which the cultures were treated with 0.15% saponin in PBS containing cOmplete Mini EDTA-free Protease and PhosSTOP Phosphatase inhibitor cocktails (both Roche) for 10 min at 4°C to lyse the host erythrocytes ...
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bioRxiv - Molecular Biology 2024Quote: ... after which the cultures were treated with 0.15% saponin in PBS containing cOmplete Mini EDTA-free Protease and PhosSTOP Phosphatase inhibitor cocktails (both Roche) for 10 min at 4°C to lyse the host erythrocytes ...
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bioRxiv - Cancer Biology 2024Quote: ... or protein extraction (50 mM Tris-HCl, 1% NP40, 0.15 M NaCl, pH 8) supplemented with phosphatase (PhoStop, Roche) and protease inhibitors (Complete ...
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bioRxiv - Cancer Biology 2024Quote: Total proteins were extracted from cultured NB cells using RIPA buffer supplemented with protease and phosphatase inhibitor cocktail (Roche). The cell lysates were pre-cleared with protein A or protein G beads (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with a protease and phosphatase inhibitors (PhosSTOP tablet Roche #04906837001, cOmplete EDTA-free Roche #11873580001, 50 mM NaF). Lysis was performed directly in the cell culture plates using a cell scraper ...
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bioRxiv - Cancer Biology 2022Quote: ... using the gRNA_enrichment1_fw (5’-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCTTGTG-GAAAGGACGAAACACCG-3’) and gRNA_enrichment1_rv (5’-CTACACGAC-GCTCTTCCGATCT-3’) primers and the 2x KAPA HiFi HotStart Ready Mix (Roche, Cat. No. KK2601). In a subsequent PCR ...
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bioRxiv - Microbiology 2022Quote: The V4 hypervariable region of the 16S rRNA gene was amplified with the primer pair 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3) on a Fast Start High Fidelity PCR System (Roche, PQ) using the following conditions ...
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bioRxiv - Cell Biology 2019Quote: ... and levels of p24 were determined by ELISA as previously described (65) The SEAP levels of the supernatants were determined by a chemiluminescent method (Roche) and were used to normalize the differences in transfection efficiencies.
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bioRxiv - Immunology 2020Quote: ... To quantify NETs we measured HNE-DNA complexes using ELISA specific for HNE and anti-DNA Ab of Cell Death Detection ELISAPLUS (Roche). Briefly ...
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bioRxiv - Biochemistry 2020Quote: RT activity was measured using an ELISA-based colorimetric reverse transcriptase activity assay (Catalog No. 11468120910, Roche Diagnostics, Indianapolis, IN). The HEPES integration buffer was used for the reaction ...
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bioRxiv - Cancer Biology 2020Quote: ... An overnight blunt ligation was induced for the 5’ of exon 1 and the 3’ of exon 3 (5 U T4 DNA ligase (Roche, Basel, Switzerland)) at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Cell Biology 2021Quote: ... and 3× EDTA-free complete protease inhibitor cocktail (Roche). Lysates were briefly sonicated until the protein solution was clear ...