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Citations for Roche :
2201 - 2250 of 7758 citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... HEK293T and A549 cells were lysed in ice-cold RIPA lysis buffer containing complete protease inhibitor and phosphatase inhibitor cocktails (Roche), resolved by SDS-PAGE ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) and 5% (v/v) glycerol and Supplementarylemented with a protease inhibitor cocktail tablet and a phosphatase inhibitor tablet (Roche). Lysates were briefly sonicated on ice and clarified by centrifuged at 20,817g for 20 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... N’-tetraacetic acid (EGTA) and 5% (v/v) glycerol and supplemented with a protease inhibitor cocktail tablet and a phosphatase inhibitor tablet (Roche). Lysates were briefly sonicated on ice and clarified by centrifuged at 20,817g for 20 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: Western blot lysates were produced in RIPA buffer (Pierce #89900) supplemented with cOmplete Mini EDTA-free protease inhibitor cocktail and PhoshoStop phosphatase inhibitor tabs (Roche) and 1 mM DTT at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice in cold 1× PBS and harvested in 2% SDS in 50 mM HEPES buffer with freshly added protease and phosphatase inhibitors (Roche). Cell lysates were sonicated and centrifuged for 15 min at 12000 ×g to remove the insoluble fraction ...
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bioRxiv - Immunology 2022Quote: ... 90 μl of faecal suspension was mixed with 10 μl (10x) buffer and 0.5 μl (0.5 U) calf intestinal phosphatase (APMB-RO, Roche, Sigma-Aldrich) and incubated at 37 °C for 60 min ...
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bioRxiv - Physiology 2022Quote: ... the sections were blocked in TS7.5 containing 1% Blocking Reagent for 1 hr and then incubated with alkaline phosphatase-conjugated anti-digoxigenin sheep antibody (1:1000; 11093274910, Roche) and anti-EP3R rabbit antibody in this blocking solution overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were hybridised with monoclonal antibodies against GFP followed by anti-mouse alkaline-phosphatase conjugated antibody and signals were developed using NBT-BCIP (Roche).
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bioRxiv - Neuroscience 2022Quote: ... eIF2αS/S and eIF2αA/A MEFs transduced with TauRD-YFP and Biosensor cell transfected with wild-type and PS19 brain lysates were lysed with SDS lysis buffer (2% SDS in PBS containing protease and phosphatase inhibitors (11836153001, Roche)) or RIPA buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... Hybridization signals were detected with anti-mouse secondary antibody labeled with alkaline phosphatase using NBT/BCIP substrates (Roche Biochemical, Mannheim).
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bioRxiv - Biochemistry 2022Quote: ... Cells were scraped with a spatula and lysed in RIPA buffer supplemented with protease and phosphatase inhibitors (cOmplete and phosSTOP, Roche) on ice for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... cells were lysed in Western Blot (WB) lysis buffer (1% SDS, 10 mM Tris, 1 mM EDTA, phosphatase and protease inhibitors (Roche, 04906837001 and 04693159001 ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was sonicated in homogenization buffer (1% SDS, 50 mM Tris pH 7.4, 2 mM EDTA, protease and phosphatase inhibitors (Roche 11873580001; Roche 04906837001), heated at 95° C for 5 minutes ...
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bioRxiv - Pathology 2024Quote: ... The proteins were redissolved in 8 M Urea/50 mM TEAB buffer containing protease- and phosphatase inhibitors (EDTA free cOmpleteTM, PhosSTOPTM Roche) and treated 30 minutes at 37 °C with 50 units/100 µg tissue benzonase (Sigma Aldrich ...
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bioRxiv - Pathology 2024Quote: Tissues were homogenized using RIPA buffer and a TissueLyser in presence of protease and phosphatase inhibitors (Inhibitor cocktail (Roche, Switzerland) and 1μM sodium ortovanadate) ...
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bioRxiv - Cancer Biology 2024Quote: ... each supplemented with protease and phosphatase inhibitors (2X cOmplete, EDTA-free, Protease Inhibitor Cocktail [Roche]; 1X PhosSTOP Phosphatase Inhibitor [Roche]; 100 µg/mL Pefabloc SC [Roche]), while incubating on ice for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... All lysis buffers were supplemented with phosphatase inhibitors NaF (10 mM) and Na3VO4 (1 mM) along with 1X protease inhibitor cocktail (ROCHE cOmplete Protease Inhibitor Cocktail Cat # 1169498001 ...
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bioRxiv - Neuroscience 2024Quote: ... DIG-labeled hybrids were detected with an anti-DIG antibody from sheep conjugated with alkaline phosphatase as recommended by the manufacturer (Roche) by incubating the slice overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were incubated in ice-cold RIPA lysis buffer (Thermo) supplemented with an EDTA-free protease inhibitor cocktail and phosphatase inhibitor (Roche). Plates were placed in a rocking platform in the cold room for 20 minutes and scraped ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated overnight at 4 °C with an alkaline phosphatase-conjugated anti-digoxigenin antibody (1:2500-1:4000, Roche). To visualize the RNA-probe binding ...
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bioRxiv - Cancer Biology 2024Quote: ... PDOs were pooled from 6 wells and incubated for 1.5 hours on ice in Cell Recovery Buffer supplemented with phosphatase inhibitors (Roche, 4906845001). PDOs were lysed using RIPA buffer (Boston BioProducts ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with PBS and lysed in radioimmunoprecipitation assay (RIPA) buffer supplemented with protease (SERVA) and phosphatase inhibitors (Roche). The protein concentration was determined using a Bicinchoninic Acid Protein (BCA ...
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bioRxiv - Plant Biology 2023Quote: ... Detection of alkaline phosphatase was carried out for ∼1 h using NBT/BCIP ready-to-use stock solution (La Roche) diluted in 1× washing buffer.
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bioRxiv - Neuroscience 2024Quote: ... Whole mount larvae and adult brain sections layered on glass slides were blocked for at least one hour in PBT with 2 mg/ml bovine serum albumin and 2% sheep serum at room temperature and then incubated overnight at 4°C with alkaline phosphatase-coupled anti-DIG antiserum (11093274910, Roche) diluted 1/5000 in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked for 30 min and then incubated in an alkaline phosphatase conjugated anti-DIG antibody (1:600, Roche). Slides were then washed and developed overnight in BCIP/NBT chromogen (Perkin Elmer) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then subjected to a transcardial perfusion with calcium-free Tyrode’s solution containing protein phosphatase-inhibitors (+PPIs) (1 tablet PhosSTOP per 10 ml) (Roche 04906845001) followed by Zamboni’s fixative containing 4% paraformaldehyde and 0.2% picric acid in 0.1 M phosphate buffer pH 7.4 +PPIs ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was homogenized in a ‘homogenization buffer’ (Sucrose, 100 mM EGTA, 10 mM Tris, protease and phosphatase inhibitors (Roche), pH 8.1 ...
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bioRxiv - Biochemistry 2022Quote: Cells were washed and harvested in PBS 1x, lysed in RIPA buffer 1x (Thermo, 88900) containing phosphatase and protease inhibitors (Roche). Lysates were centrifuged at 15,000 g to separate soluble and pellet fractions ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were washed with high stringency (0.5× SSC, 70°C) and developed with alkaline phosphatase-conjugated anti-DIG antibody (Roche, Switzerland) with CDP-Star substrate in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed twice with 10 ml of cold 1x PBS supplemented with proteinase inhibitors and phosphatase inhibitors (Roche # 4693132001) and the pellets were snap frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Two brains were transferred to a pre-chilled 30-mL glass tube Wheaton homogenizer containing 5 mL of cold ‘solubilisation-plus’ buffer (solubilisation buffer with 1X COMPLETE protease inhibitor and 1X PhosSTOP phosphatase inhibitor (Roche)) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysis buffer (150 mM NaCl, 1 mM EDTA, 50 mM Tris-HCl, 1% NP-40 with proteases and phosphatases inhibitors (Roche)) was added directly to the wells and cells were scraped and collected in pre-cooled Eppendorf tubes followed by sonification for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated in an anti-digoxigenin antibody (alkaline-phosphatase-coupled anti-digoxigenin; RRID:AB_514497, diluted 1:3,500; Roche Diagnostics; Table 2) for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... 2% sheep serum in PBST) for one hour and overnight at 4 °C with alkaline phosphatase-conjugated anti-DIG antibodies (Roche) diluted at 1/4000 in the blocking solution ...
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bioRxiv - Biochemistry 2023Quote: ... SRA01/04 cell lysates were obtained using RIPA lysis buffer (Beyotime) supplemented immediately before use with 10 μl ml-1 phosphatase and protease inhibitors (PhosSTOPTM, Roche). The bicinchoninic acid assay (Beyotime ...
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bioRxiv - Biochemistry 2023Quote: ... cells were lysed in Homogenization buffer (20 mM Tris, pH 7.4, 10mM EDTA, supplemented with phosphatase inhibitors (Phosphostop, Roche, Germany), and protease inhibitors (cOmplete ...
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bioRxiv - Developmental Biology 2023Quote: ... Hybridization was carried out at 600C overnight and the slides were washed and probed with an alkaline phosphatase-conjugated anti-DIG antibody (Roche) at 1:2000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... Alkaline phosphatase activity was detected in the dark in AP buffer supplemented with 45 mg/ml 4-nitrobluetetrazolium chloride (NBT, Roche) and 35 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibody incubation was performed for 16 h at 4°C in AB buffer supplemented with 1% v/v Fetal Calf Serum and anti-DIG antibody coupled to alkaline phosphatase (1∶5000 dilution; Roche). Sections were then washed thoroughly in AB and equilibrated in alkaline phosphatase buffer (AP - 0.1 M Tris–HCl pH ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium dodecyl sulfate) containing protease and phosphatase inhibitors (Pierce Protease Inhibitor Mini Tablet, EDTA-free, Thermo Scientific; PhosSTOP, Roche) for 45 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: Protein for Western blotting of components of trans-synaptic bridges was extracted as described above except that larvae were anaesthetized on ice and RIPA lysis buffer was supplemented with phosphatase inhibitors (PhosSTOP, Roche). 30 μg of total protein were loaded per well and Ponceau S staining was used to check total protein levels on the membrane ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were hybridized with digoxigenin (DIG)-labeled riboprobes overnight at 65°C and incubated overnight with 1/2000 alkaline phosphatase-conjugated anti-DIG Fab fragment (Roche) at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... They were then lysed in radio-immunoprecipitation assay (RIPA) buffer supplemented with PhosSTOP phosphatase and CompleteMini protease inhibitors (Roche, Switzerland) for protein extraction ...
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bioRxiv - Immunology 2023Quote: ... and lysed in RIPA buffer (CST; cat# 9806S) or NP-40 lysis buffer containing PhosSTOP phosphatase inhibitor (Roche; cat# 04906845001) and EDTA-free protease inhibitor (BioShop ...
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bioRxiv - Neuroscience 2023Quote: Lysates were prepared from 10 homogenised adult or pharate brains in lysis buffer with a phosphatase–protease inhibitor cocktail (Roche) on ice and suspended in LDS sample buffer and reducing agent (Invitrogen) ...
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bioRxiv - Pathology 2023Quote: ... The preparations were washed with buffer 1 and incubated for 2h at 37°C with an anti-digoxigenin antibody conjugated to alkaline phosphatase (1:200 in blocking solution; 11093274910, Roche). Next ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were homogenized with a lysis buffer (0.32M Sucrose, 50 mM Tris Base and protease/phosphatase inhibitor cocktail (Roche, Germany) using a sonicator (Sonifier SFX 150 ...
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bioRxiv - Microbiology 2023Quote: ... 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT, 0.5 mM AEBSF, 1X Roche protease inhibitor, 1X Roche phosphatase inhibitor) followed by a 30’incubation on ice ...
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bioRxiv - Microbiology 2023Quote: ... Nuclear pellets were resuspended in shearing buffer (0.1% SDS, 1 mM EDTA, 10 mM Tris-HCl pH 7.5, 1X Roche protease inhibitor, and 1X Roche phosphatase inhibitor) and transferred into 130 μl Covaris tubes (Covaris ...