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Citations for Roche :
1901 - 1950 of 7357 citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The F9 teratocarcinoma cells were grown in DMEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The F9 teratocarcinoma cells were grown in DMEM (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... and 100 U/mL human interleukin 2 (IL-2) (Roche) (Munoz et al. ...
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bioRxiv - Biophysics 2019Quote: ... and 30 IU/mL human interleukin-2 (hIL-2) (Roche).
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bioRxiv - Cell Biology 2021Quote: ... human NEMO (5 µg) and ATP (2 mM) (Roche, 10519979000) were incubated at 37°C for the indicated time in a buffer containing 150 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... coated with 5 µg/ml human plasma fibronectin (Roche, 11080938001) in growth medium overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... and human Ki67 rabbit monoclonal antibody (Roche Diagnostics, 790-4286). Images were captured with VENTANA iScan HT (Roche Diagnostics ...
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bioRxiv - Immunology 2023Quote: ... 100 IU/ml of recombinant human IL-2 (Roche Diagnostics) or 10 μg/ml of SIVmac251 Env gp130 (ImmunoDx) ...
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bioRxiv - Immunology 2023Quote: ... and 30 IU/mL human interleukin-2 (hIL-2) (Roche). To ectopically express Lifeact-eGFP or Lamp1-eGFP in CTLs ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.01 mg/mL of human insulin (Roche, Indianapolis, IN, USA), 10 mM HEPES (Corning ...
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bioRxiv - Molecular Biology 2020Quote: ... 1X protease inhibitors (Complete mini EDTA-free mixture [Roche Applied Science], 1X phosphatase inhibitor mixture [PhosSTOP, Roche Applied Science]). After centrifugation (20,000 × g for 10 min at 4°C) ...
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bioRxiv - Molecular Biology 2020Quote: ... and then lysed with IP lysis buffer (0.5% NP-40, 150 mM NaCl, 50 mM Tris HCl, pH 7.5, protease inhibitors [cOmplete, Roche], and phosphatase inhibitors [PhosStop, Roche]). Cell lysates were clarified by serial centrifugation (600 ×g for 3 min and 9300 ×g for 10 min ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The lysis was performed in RIPA buffer (1% SDS, 10 mM Tris pH 8.0, containing protease and phosphatase inhibitor cocktail tablets (Roche)) ...
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bioRxiv - Developmental Biology 2020Quote: ... they were hybridized overnight at 65°C for three nights and incubated overnight with 1/2,000 alkaline-phosphatase conjugated with anti-DIG Fab fragment (Roche) at 4°C for three nights ...
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bioRxiv - Developmental Biology 2020Quote: ... for 1 h and then incubated overnight at 4 °C in block solution with diluted DIG-antibodies (1:5,000) conjugated with alkaline phosphatase (AP) (Roche). To visualize WISH signal ...
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bioRxiv - Cell Biology 2022Quote: ... cells were then harvested on ice lysed with M2-Lysis buffer72 supplemented with phosphatase and protease inhibitors tablets (Roche), and lysates ran on 15% SDS-PAGE at constant voltage (75V for fifteen minutes followed by 90V for 2 hours) ...
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bioRxiv - Neuroscience 2021Quote: ... 1.5 mM, 10% glycerol, pH 7.4, containing phosphatase inhibitors (10mM NaF, 1mM Na3VO4) and protease inhibitors (cOmplete®, Roche). Protein concentration was adjusted to 10 mg/ml by the Bradford method (Pierce ...
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bioRxiv - Neuroscience 2019Quote: ... 0.32M sucrose) supplemented with protease and phosphatase inhibitor tablets (Complete Mini, EDTA-free and PhosSTOP; Roche Diagnostics, Mannheim, Germany), using A Power Gen 125 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... containing phosphatase inhibitors (1 mM NaF and 0.1 mM Na3VO4) and protease inhibitors (cOmplete mini cocktail, EDTA-free, Roche). Immunoblots were carried out using the following antibodies ...
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bioRxiv - Cell Biology 2019Quote: HeLa cells were lysed in 1% NP-40 lysis buffer (150 mM NaCl, 20 mM HEPES, 0.5 mM EDTA) containing complete protease and phosphatase inhibitor cocktail (Roche). 10 mg of total protein lysate were incubated for 3 h with protein A Dynabeads (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... buffer (10 mM Tris [pH 7.5], 1 mM EDTA, 250 mM sucrose, 1 mM dithiothreitol [DTT], plus protease and phosphatase inhibitor cocktail [Roche]). Homogenates were centrifuged at 100,000 ×g for 1 h at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Signals were detected using anti-mouse secondary antibodies conjugated with alkaline phosphatase using BCIP/NBT substrates (Roche Biochemical, Mannheim). Similarly ...
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bioRxiv - Immunology 2021Quote: ... coated with H-2Kb-OVA and ICAM-1 at a 1:1 ratio for various times at 37 ºC and immediately lysed in 2× cold lysis buffer containing phosphatase inhibitors (1 mM NaF and 0.1 mM Na3VO4) and protease inhibitors (cOmplete mini cocktail, EDTA-free, Roche). Activation of PI3K and MAP kinase signaling was assessed by immunoblot for pAkt (Phospho-Akt (Ser473 ...
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bioRxiv - Neuroscience 2020Quote: ... The signals were visualized with a nonradioactive detection system using anti-digoxigenin Fab fragment conjugated to alkaline phosphatase (Roche).
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed in 1% NP-40 lysis buffer (150 mM NaCl, 20 mM HEPES, 0.5 mM EDTA) containing complete protease and phosphatase inhibitor cocktail (Roche). Total cell extracts were incubated for 3 h with 20 μl protein A Dynabeads (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.9, 10 mM KCl, 0.1 mM EDTA, 0.3 % NP-40 and 1 x protease and phosphatase inhibitors, Roche, USA) by passing through a 23G needle and syringe 30 times and then left to stand on ice for 5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.1% SDS and 0.5% sodium deoxycholate supplemented with EDTA-free protease inhibitor and PhosSTOP phosphatase inhibitor (Roche Applied Science), then centrifuged at 13,000 rpm for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The pelleted cells were homogenized in 1X RIPA lysis buffer containing freshly added phosphatase and protease inhibitor cocktail (Roche). To remove cellular debris ...
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bioRxiv - Neuroscience 2022Quote: ... The human tissues were homogenized in TBS (20 mM Tris-HCl, 140 mM NaCl, pH 7.5) containing protease and phosphatase inhibitors (Roche). Homogenates were ultracentrifuged (4 °C for 60 min ...
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bioRxiv - Developmental Biology 2022Quote: ... The alkaline phosphatase activity was visualized by incubating the specimens in NTMT containing 0.07 mg/ml nitroblue-tetrazolium chloride (Roche), and 0.035 mg/ml 5-bromo-4-chloro-3-indolyphosphatase (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... after which the cultures were treated with 0.15% saponin in PBS containing cOmplete Mini EDTA-free Protease and PhosSTOP Phosphatase inhibitor cocktails (both Roche) for 10 min at 4°C to lyse the host erythrocytes.
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bioRxiv - Microbiology 2022Quote: ... supplemented with 0.5% Nonidet P40 substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)) ...
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bioRxiv - Cell Biology 2022Quote: ... human tissue was homogenized (Dounce homogenizer) in TBS (20 mM Tris–HCl, 140 mM NaCl, pH 7.5) containing protease and phosphatase inhibitors (Roche). Homogenates were ultracentrifuged (4 °C for 60 min ...
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bioRxiv - Cell Biology 2022Quote: ... In situ hybridization signals in whole-mount embryos were detected using anti-DIG alkaline phosphatase (AP)-conjugated antibody (Roche) with an AP substrate ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% SDS and 1% sodium deoxycholate) supplemented with protease and phosphatase inhibitors (100 mM PMSF and PhosSTOP tablet (Roche)) ...
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bioRxiv - Neuroscience 2020Quote: Lysates for whole cell protein analysis were obtained by resuspending cells with Tris-buffered Saline (TBS) supplemented with 0.05% NP40 with the addition of phosphatase and protease inhibitors (Roche) on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... before treating with lysis buffer (10 mM Tris pH 7.5, 2 mM EDTA) containing a protease and phosphatase inhibitor cocktail (Roche). Chromatin-enriched protein fractions were prepared essentially as described (Mailand and Diffley ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were re-suspended in 2 mL of lysoIP buffer (50 mM KCl, 100 mM KH2PO4, 100 mM K2HPO4 pH7.2) supplemented with protease and phosphatase inhibitors (Roche). The cells were transferred to a glass homogenizer and dounced using 25 strokes ...
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bioRxiv - Cell Biology 2021Quote: Cell pellets or mouse tissues were resuspended in ice-cold 8 M urea buffer (8 M urea, 50 mM Tris pH 7.4, 50 mM NaCl) supplemented with protease and phosphatase inhibitors (Roche). The resuspended samples were sonicated ...
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Noradrenergic alpha-2A receptor activation suppresses courtship vocalization in male Japanese quail.bioRxiv - Animal Behavior and Cognition 2021Quote: ... The slides were incubated overnight with a 1:5,000 dilution of alkaline phosphatase-conjugated anti-DIG antibody (Roche Diagnostics). After the slides had been washed in in a solution of 0.1 M Tris (pH 7.5 ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... pH 8.0) augmented with protease and phosphatase inhibitors (1:100 HALT PI cocktail, Thermofisher Sci Cat# 78440; or Roche cOmplete ULTRA [Cat# 05892970001] plus phosStop [Cat# 4906845001] tablets) ...
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bioRxiv - Cell Biology 2022Quote: ... 10mM Tris-HCl pH 8, 1 mM DTT, 0.2% NP-40, 1x protease inhibitor [Roche], 1x phosphatase inhibitor [Roche]), sonicated and centrifugated for 10 minutes at 14,000 RPM at 4°C ...
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bioRxiv - Neuroscience 2019Quote: Microdissections were triturated in 100 μl of lysis buffer (150 mM NaCl, 1% Triton, 100 mM Tris, pH 7.4, 10 mM EDTA, 1X protease/phosphatase inhibitor (Roche)) per 10 mg of tissue and incubated 10 min on ice ...
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bioRxiv - Plant Biology 2019Quote: ... Seedlings were ground in liquid nitrogen and homogenized in ice-cold extraction buffer (25 mM HEPES-KOH pH 7.5, 10 mM MgCl2, protease inhibitor cocktail [complete Mini, Roche], phosphatase inhibitor cocktail [phosSTOP, Roche]). Samples were incubated on ice for 10 min and then centrifuged for 15 min at 16,000 g ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were lysed with TNE (Tris) buffer containing protease (Complete Mini) and phosphatase inhibitors (PhosStop Roche, 50mM Sodium Fluoride). Protein concentrations were determined by Bradford assay and lysates stored at −80°C until use.
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bioRxiv - Zoology 2020Quote: ... 0.25% deoxycholate) supplemented with protease and a phosphatase inhibitor cocktail (MilliporeSigma, Burlington, Massachusetts, USA and Roche, Basel, Switzerland; respectively). Each lysate was confirmed to express the appropriate recombinant protein at the expected size using an anti-V5 antibody produced in rabbit (Sigma V8137) ...
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bioRxiv - Molecular Biology 2020Quote: ... The sections were then rinsed with 2× SSC and 0.2× SSC and treated with alkaline phosphatase– conjugated anti-digoxigenin (anti-DIG, 1:500; Roche) for 2 h at room temperature ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... 1% Triton X-100 plus protease and phosphatase inhibitor cocktail tablets (Complete mini, EDTA-free, Phos-STOP from Roche). Insoluble cell material was rejected by centrifugation ...
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bioRxiv - Genomics 2020Quote: ... Protein lysates were prepared by manually homogenizing frozen tissue in RIPA buffer containing protease and phosphatase inhibitor cocktails (Roche). Proteins were separated on a 4-20% TGX SDS-PAGE gel (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: Proteins were extracted using Tris-buffered Saline (TBS) with 0.5% NP40 protein extraction buffer containing protease and phosphatase inhibitors (Roche), on ice ...