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Citations for Roche :
1801 - 1850 of 7357 citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Universal probe library (UPL) probe #3 (Roche) and following primers were used for analysis of wtAAV2 ...
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bioRxiv - Developmental Biology 2022Quote: ... 3 µL 10% Tween-20 (Roche 11332465001), freshly add 3 µL 1:1 water diluted digitonin (Promega G9441) ...
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bioRxiv - Plant Biology 2019Quote: ... 3 uL of DNaseI (10U/ul Roche), 8 uL buffer (200 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... 3 U ml-1 Dispase II (Roche), and 10 mM CaCl2 for 45 min at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 3 U ml-1 Dispase II (Roche), and 5 mM CaCl2 for 45 min at 37 °C with shaking ...
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bioRxiv - Neuroscience 2023Quote: ... retigabine (3 mg/kg, Roche Pharmaceuticals, CH), nicotine (5 mg/kg ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 mg/mL Dispase II (Roche, 04942078001), and 0.1 mg/mL DNase I (Sigma ...
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bioRxiv - Pathology 2023Quote: Sections were stained for CD8 and pan-cytokeratin (CK) in a dual chromogenic IHC assay on a Discovery ULTRA platform (Roche Tissue Diagnostics (RTD), Tucson ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.01 mg/mL human insulin (Roche, Indianapolis, IN, USA), 100 IU penicillin (Mediatech) ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... and human recombinant IL-2 (30 U/ml, Roche) for 72 h.
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bioRxiv - Cancer Biology 2023Quote: ... human PR rabbit monoclonal antibody (Roche Diagnostics, 790-4296), and human Ki67 rabbit monoclonal antibody (Roche Diagnostics ...
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bioRxiv - Immunology 2023Quote: ... and 10 ng/ml recombinant human IL-2 (Roche). For activation of murine CD4+ T cells ...
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bioRxiv - Immunology 2023Quote: ... human interleukin-2 (IL-2) (10 IU/ml, Roche), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... 5’-CAGACACGCAGACTCTTTC-3’) and 2.5 µl reverse primer (10 µM; 5’-CTAAAGATGTGTGTCTTCCTCA-3’) using the Expand Long Template PCR System (Roche). The PCR conditions were 35 cycles at 95°C for 30 sec ...
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bioRxiv - Cell Biology 2022Quote: ... with 0.5μM of each primer (Forward: 5’-GGGAGCCTGATCCTATCGTT-3’; Reverse: 5’-TCCCAAAGCACAGCTTCC-3’) and 50nM Universal ProbeLibrary Probe #67 (Roche). RT-PCR was performed in QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... RNA was reverse transcribed using Transcriptor High Fidelity cDNA Synthesis Kit and a specific 3’-UTR DENV-1 primer (Roche Applied Science, Mannheim, Germany), d1a5B 5’-AGAACCTGTTGATTCAACRGC-3’ (62) ...
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bioRxiv - Plant Biology 2021Quote: ... was isolated from the cDNA of juvenile cluster root tissues from P-deficient plants using the 5’/3’ RACE Kit (2nd generation, Roche Diagnostics S.p.a., Monza, Italy) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR primer for generating the probe was 5’-GGTTCTGTGATTGAGTGTTTGGATCTCCCTGCG-3’ with a single-end CY3 tag generated using the DIG RNA Labeling Kit (Roche Applied Science, Penzberg, Germany) based on the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries for target enrichment of ∼3 Mb of Lupinus angustifolius genomic material were produced using the Roche Sequencing Solutions’ ‘SeqCap EZ – HyperPlus’ kit (Roche Sequencing Solutions, Pleasanton, CA) with 200 ng/L of input DNA.
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription (RT) was performed from 2-3 µg of RNA with the Transcriptor First Strand cDNA Synthesis Kit (Roche Life Sciences, Basel, Switzerland) using random hexamer primers ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were incubated in alkaline phosphatase-conjugated anti-DIG (Digoxygenin) antibody Fab fragments (1:5000; Roche, catalog #12486523) at 1:5000 in the blocking buffer and incubated at 4°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1% SDS and 0.5% sodium deoxycholate with PhosStop Phosphatase-Inhibitor Cocktail tablet and protease-inhibitor cocktail tablet (Roche). The homogenate was centrifuged at 4°C for 10 min at 14,000g and the supernatant was used for western blot analysis ...
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bioRxiv - Immunology 2019Quote: ... EDTA/DTT-treated IECs were lysed in radioimmunoprecipitation assay buffer (RIPA) supplemented with protease and phosphatase inhibitors (Roche). Equal volumes of 2X Laemmli buffer (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: Hippocampi were collected 24 h after KA treatment and protein extracts were obtained by processing samples with lysis buffer (Tris-Base 0.5 M, pH 7.4; SDS 10 %) containing phosphatase and proteinase inhibitors (Roche). Lysates were clarified by centrifugation at 14000 x g for 10 min and protein concentration was measured using the bicinchoninic acid assay ...
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bioRxiv - Cancer Biology 2021Quote: Cell pellets were lysed with CST lysis buffer (Cat #9803) that was supplemented with phosphatase (Roche, Cat #04906845001) and protease inhibitors (Roche ...
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bioRxiv - Bioengineering 2021Quote: ... After the addition of the chemiluminescent substrate for alkaline phosphatase CDP star® ready to use reagent (Roche) at a 1:4 ratio ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated with an alkaline phosphatase-conjugated anti-digoxigenin antibody (1:1500 in blocking solution; Roche, Switzerland) overnight at RT ...
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bioRxiv - Neuroscience 2020Quote: ... 25 mM Tris-HCl pH 7.6) containing phosphatase and protease inhibitors (PhosSTOP™, cOmplete™; both from Roche). Protein concentration was determined with the Pierce™ BCA Protein Assay Kit (Pierce™ ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates were collected in RIPA buffer supplemented with proteinase and phosphatase inhibitors (Roche 11697498001, Roche 04906937001). Protein was separated by electrophoresis using 4- 12% gradient Bis-Tris NuPAGE gels (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates were collected in RIPA buffer supplemented with proteinase and phosphatase inhibitors (Roche 11697498001, Roche 04906937001). Protein was separated by electrophoresis using 4- 12% gradient Bis-Tris NuPAGE gels (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were washed twice with ice-cold PBS containing cOmpleteTM protease inhibitor and phosSTOPTM phosphatase inhibitor cocktails (Roche). Cells were then gently lifted from membranes with cell scrapers (Corning ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5mM EDTA, 0.5% NP40, 0.5% Triton-X and a cocktail of protease and phosphatase inhibitors (1mM orthovanadate, cOmplete (Roche), PHOSstop (Roche)) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were pelleted then washed once with 250 uL TAP extraction buffer (40 mM HEPES pH 7.5, 10% Glycerol, 350 mM NaCl, 0.1% Tween-20, phosphatase inhibitor cocktail and proteinase inhibitor cocktail from Roche). Cell pellets were resuspended in 180 uL modified 2X SDS buffer then boiled at 100 °C for 4-5 minutes ...
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bioRxiv - Genetics 2022Quote: ... Overnight incubation with Anti-Dig antibody conjugated to alkaline phosphatase (1:5,000) at 4 °C (no. 11093274910; Roche) was followed by 8 × 30 min washing steps at room temperature with TBST 2 (TBST with 0.1% Tween 20 and 0.05% levamisole–tetramisole ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... supplemented with phosphatase inhibitor (04-906-837001) and protease inhibitor (11-836-153001s) (both from Roche, Mannheim, Germany) to generate whole-cell extracts (WCEs) ...
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bioRxiv - Neuroscience 2021Quote: Tissue was dissociated with a motorized pestle (Argos Technologies) in 0.3-0.5mL ice cold PBS treated with protease and phosphatase inhibitors (cOmplete & PhosSTOP; Roche). For RNA isolation ...
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bioRxiv - Microbiology 2020Quote: Whole-cell extracts were obtained from liver tissue or cell pellets lysed in buffer (0.1%, sodium dodecyl sulfate, 0.1% sodium deoxycholate, 1% IGEPAL) supplemented with Protease and Phosphatase Inhibitor cocktails (Roche). Protein extracts were separated by SDS-polyacrylamide gel electrophoresis then transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2021Quote: ... containing Phosphatase (1 tablet per 3.3ml lysis buffer) and Protease (1 tablet per 16.67ml lysis buffer) inhibitor (Roche cOmplete tablets ...
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bioRxiv - Molecular Biology 2019Quote: Mitochondria isolated from 3 confluent 150 mm dishes were resuspended in the 200 μl of lysis NP40 buffer (0.3% NP40, 150mMNaCl, 50mMTris pH 7.5) supplemented with protease and phosphatase inhibitors (cOmplete Roche), incubated for 20 min at +4°C and centrifuged at +4°C 10,000 RPM for 15 min ...
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bioRxiv - Immunology 2021Quote: ... the cells were thawed on ice followed by lysis using Farham lysis buffer (5 mM PIPES pH 8.0, 85mM KCl, 0.5% NP-40 supplemented with protease and phosphatase inhibitors (Roche)) made in miliQ ...
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bioRxiv - Microbiology 2021Quote: ... 10 mM Tris, pH 7.5, 1 mM EDTA, 0.5% Nonidet P-40, protease and phosphatase inhibitor cocktail [Roche]). Cell lysates were denatured in Laemmli sample buffer and incubated for 5 min at 95°C ...
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bioRxiv - Physiology 2020Quote: ... 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, protease inhibitors [Roche], phosphatase inhibitors [Roche]). Lysates were incubated for 15 minutes on ice and centrifuged twice at 15,000 x g for 20 minutes at 4°C to remove insoluble material ...
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bioRxiv - Neuroscience 2021Quote: ... The cell pellets were lysed in 0.25M Sucrose/1xTBS buffer (pH 7.4) supplemented with 1x phosphatase inhibitors (Roche) in a manual glass homogenizer ...
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bioRxiv - Immunology 2022Quote: ... freshly supplemented with PhosSTOP™ inhibitor tablets for phosphatase and cOmplete™ Protease-Inhibitor Cocktail (Roche, Basel, Switzerland). 10 μg of protein per sample were collected and normalized according to BCA assays ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 0.5 mM EDTA] containing protease and phosphatase inhibitors (Roche 04 693 123 004 and 04 906 837 001). Supernatant was centrifuged at 740g for 5 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... 50mM Tris (pH 8.5-8.8)) containing protease and phosphatase inhibitors (cOmplete Mini, PhosSTOP, Roche; 2mM Sodium Orthovanadate, NEB), sonicated to shear chromatin and centrifuged to remove cellular debris ...
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bioRxiv - Microbiology 2019Quote: ... cells were harvested at 0 to 7 DPI in RIPA buffer containing protease and phosphatase inhibitor cocktails (Roche). Protein concentrations were determined by bicinchoninic acid (BCA ...
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bioRxiv - Physiology 2020Quote: ... and 1 mM phenylmethylsulfonyl fluoride (PMSF)) supplemented with EDTA-free protease inhibitor cocktail and phosphatase inhibitors cocktail (Roche). Tissue lysates were centrifuged at 14,000 rpm for 20 min at 4°C twice before using the supernatant for western blot studies ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated overnight at 4 °C with alkaline phosphatase–conjugated anti-digoxigenin antibodies in TBTX (1:2000; Roche). Following extensive washing in TBTX ...
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bioRxiv - Molecular Biology 2020Quote: ... The homogenized pellets were re-suspended in isolation buffer with protease and phosphatase inhibitor cocktails (Roche, Indianapolis, IN). The protein concentration was measured by BCA protein assay kit (Thermo Scientific ...