Labshake search
Citations for Roche :
2151 - 2200 of 7357 citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... 20 mM Tris pH 7.5, 1% Triton X-100 supplemented with 1× protease inhibitor and 1× phosphatase inhibitor; Roche, Basel, Switzerland). Lysates were cleared by centrifugation at 10,000 r.p.m ...
-
bioRxiv - Cell Biology 2019Quote: ... pH 7.5) containing protease inhibitor cOmplete Tablets EASYpack or phosphatase inhibitor PhosSTOP EASYpack (each 1 tablet per 15 mL, Roche, Basel, Switzerland), and diluted in 6×SDS-PAGE sample loading buffer (Beyotime ...
-
bioRxiv - Neuroscience 2021Quote: ... in 500 μl lysis buffer containing 1% SDS in 1x PBS with Halt phosphatase inhibitor cocktail (Fisher: PI78420) and Complete mini EDTA-free protease inhibitor cocktail (Roche: 4693159001). Sample homogenates were then boiled on a heat block at 95°C for 10 min ...
-
bioRxiv - Neuroscience 2021Quote: ... 1% Triton-X-100) supplemented with 1x protease inhibitor (Complete mini EDTA-free, Roche, Switzerland and 1x phosphatase inhibitor (PhosStop, Roche, Switzerland) on ice ...
-
bioRxiv - Neuroscience 2021Quote: ... After that pelleted beads were gently resuspended in washing buffer containing 4xTBS, 5% NP-40, phosphatase inhibitors (10mM NaF, 1mM Na3VO4) and protease inhibitors (cOmplete®, Roche) and washed 10 times with gentle agitation ...
-
bioRxiv - Molecular Biology 2021Quote: ... were homogenized on ice with a hand-held OMNI TH homogenizer (OMNI International) with ice-cold RIPA lysis buffer containing Complete Mini Protease Inhibitor Cocktail and PhosSTOP Phosphatase Inhibitor Cocktail (Roche Diagnostics). In cell culture experiments ...
-
bioRxiv - Cell Biology 2019Quote: Cells were lysed in M-PER mammalian protein extraction reagent (Thermo, MA, USA) with protease and phosphatase inhibitors (Roche, Basel, Switzerland), and proteins were quantified using protein assays (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2019Quote: ... pH 8.0 and 10% sucrose) along with 1X PhosSTOP phosphatase and 1X cOmplete protease buffer (Roche Applied Science, Indianapolis, IN, USA). The homogenized samples were centrifuged at 4°C for 15 min at 18,000g ...
-
bioRxiv - Immunology 2021Quote: ... 1mM PMSF, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1x Roche Complete Protease Inhibitor Cocktail, and 1x Roche Phosphatase Inhibitor Cocktail). Protein concentrations were measured using a BCA kit (Pierce) ...
-
bioRxiv - Neuroscience 2021Quote: ... 0.1% Tween 20) for 20 min followed by 1 h incubation with alkaline phosphatase-anti-digoxigenin antibody (1:500, Roche, 1093274). Alkaline phosphatase activity was detected by incubating slides with Fast Red substrate (DAKO ...
-
bioRxiv - Cell Biology 2021Quote: ... They were lysed in RIPA buffer (Research products, R26200-250.0) supplemented with protease and phosphatase inhibitor cocktails (Roche, 11836170001 and 4906845001) using a Tissue-Lyser (5 times 5min at 30 shakes/second) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Animals were then re-suspended on ice in 50 µl of RIPA buffer with added phosphatase inhibitors (Cell Signalling Technologies #9806) and complete EDTA-free protease inhibitor cocktail (Roche # 04693159001) before sonication ...
-
bioRxiv - Cell Biology 2022Quote: Murine cells or aortic tissue were lysed in RIPA buffer containing complete protease inhibitor and phosphoSTOP phosphatase cocktails (Roche Applied Science). Lysate was centrifuged at 10,000 g for 10 min at 4°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 10% sheep serum] and incubated with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche Cat# 11093274910) in MABT/Block at 4°C overnight ...
-
bioRxiv - Neuroscience 2021Quote: ... proteinase K treatment was omitted and color development of alkaline phosphatase activity was carried out for 1 h with nitroblue tetrazolium chloride (Roche Diagnostics) and 5-bromo-4-chloro-3-indolyl phosphate (Roche Diagnostics ...
-
bioRxiv - Neuroscience 2020Quote: The following conjugated antibodies were used: sheep polyclonal anti-digoxigenin alkaline phosphatase (1:2000 - 1:5000; Roche Life Science, Cat#11093274910), mouse monoclonal anti-βACTIN (clone AC-15 ...
-
bioRxiv - Physiology 2020Quote: ... in lysis buffer (50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, protease inhibitors [Roche], phosphatase inhibitors [Roche]). Lysates were incubated for 15 minutes on ice and centrifuged twice at 15,000 x g for 20 minutes at 4°C to remove insoluble material ...
-
bioRxiv - Neuroscience 2022Quote: ... Brains were homogenised in HB buffer (20 mM NaP pH 7.4, 30 mM NaCl containing phosphatase inhibitors (PierceTM) and protease inhibitors (Complete, Roche Diagnostics GmbH)) ...
-
bioRxiv - Genetics 2022Quote: ... Samples were suspended in Laemmli sample buffer supplemented with a protease and phosphatase inhibitor cocktail (PIC; 1 mM PMSF, cOmplete™ Protease Inhibitor Cocktail [Roche, Base ...
-
bioRxiv - Developmental Biology 2022Quote: ... 50mM Tris-HCl [pH 7.2], 250mM NaCl, 2mM EDTA, phosphatase inhibitor mixture I and II [Calbiochem] and one tablet of Minicomplete [Roche] per 10ml) on ice ...
-
bioRxiv - Developmental Biology 2020Quote: ... pH 7.4, 150 mM NaCl, 1% NP-40, 2 mM EGTA, and complete protease and phosphatase inhibitors from Roche, Basel, Switzerland). Cell lysate was cleared by centrifugation ...
-
bioRxiv - Cancer Biology 2020Quote: Cells and tumours were lysed in RIPA buffer supplemented with protease and phosphatase inhibitors (cOmplete™ ULTRA Tablets, Mini, EASYpack Protease Inhibitor Cocktail, and PhosSTOP, both from Roche). Total lysates were resolved by SDS/PAGE and transferred to nitrocellulose membranes ...
-
bioRxiv - Molecular Biology 2020Quote: ... Signals were detected using anti-rabbit IgG conjugated with alkaline phosphatase and the color reaction substrates NBT-BCIP (Roche Biochemicals, Germany).
-
bioRxiv - Cell Biology 2019Quote: ... Brain tissue was homogenized in a teflon dounce grinder on ice in PBS with protease and phosphatase inhibitors (Roche, Indianapolis, Indiana). After homogenization ...
-
bioRxiv - Cell Biology 2019Quote: Cells were lysed in Tris-buffered saline (TBS) supplemented with 1% Triton X-100 and protease/phosphatase inhibitor cocktails (Roche Diagnostics), the insoluble material was cleared by centrifugation for 10 min at 20,000 × g ...
-
bioRxiv - Molecular Biology 2021Quote: ... washed with ice-cold PBS (w/o calcium and magnesium) and lysed immediately in RIPA-Buffer containing phosphatase inhibitor cocktail (PhosStop, Roche, USA) and protease inhibitor cocktail (HaltProtease Inhibitor Cocktail ...
-
bioRxiv - Immunology 2021Quote: ... plus phosphatase and protease inhibitors (2 mM NaVO4, 10 mM NaF, and 1 mM PMSF) and Complete protease inhibitor cocktail (Roche, CA). For tissue extracts ...
-
bioRxiv - Developmental Biology 2020Quote: ... 2 mg/ml bovine serum albumin dissolved in TBST) for 1 h and subsequently incubated with anti-DIG-Fab antibody coupled to alkaline phosphatase (Roche: 11093274910) in blocking solution (1 in 5000 ...
-
bioRxiv - Plant Biology 2022Quote: ... Total proteins from mixed samples (14N-flg22/15N-mock and 14N-mock/15N-flg22) were extracted with IP buffer 2 with 1× Protease Inhibitor (Pierce) and phosphatase inhibitor (Roche, PhosSTOP), and centrifuged for 1700g for 5 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... the frozen human tumors and normal tissues and the tumors from animal xenograft studies were lysed in RIPA lysis buffer containing protease and phosphatase inhibitors (Roche Inc), and sonicated for 20s ...
-
bioRxiv - Cell Biology 2022Quote: ... and proteins were extracted in ice-cold lysis buffer (150 mM NaCl, 2 mM EDTA, 40 mM HEPES, and 1% Triton X-100) supplemented with phosphatase (Roche #04906837001) and protease inhibitor cocktail ...
-
bioRxiv - Plant Biology 2022Quote: ... and 1X protease inhibitor cocktail) with a combination of phosphatase inhibitors (20mM NaF, 1mM Na2MoO4, 10mM Na3VO4, 50mM β-glycerophosphate and 1X PhosStop cocktail (Roche) to preserve EIR1 phosphorylation status ...
-
bioRxiv - Neuroscience 2022Quote: ... Each region was homogenized using Ribolyser for 5 min in 500uL lysis buffer (140mM NaCl, 20mM TrisHCl, pH 7.5, protease inhibitors [complete Mini, Roche], phosphatase inhibitors [PhosphoSTOP, Roche] in PBS), and centrifuged at 15000g for 30 min at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... supplemented with protease and phosphatase inhibitors (1 tablet per 5ml buffer of cOmplete™, mini, EDTA-free protease inhibitor cocktail [Roche] ...
-
bioRxiv - Immunology 2023Quote: ... 1 mM NaF) with EDTA-free protease inhibitors (cOmplete™ Tablets) and phosphatase inhibitors (PhosSTOP™, both Roche Diagnostics GmbH, Germany) for 1 hour at 4°C under rotation ...
-
bioRxiv - Neuroscience 2022Quote: Mouse hippocampal proteins from PBS control and Aβ oligomer (JD1 positive) treated brain slices were extracted with 2% SDS (in PBS) containing protease and phosphatase inhibitors (Roche, Switzerland) followed by centrifugation at 14000 x g for 15min to precipitate any insoluble material ...
-
bioRxiv - Neuroscience 2023Quote: ... was performed in HB buffer (20 mM Na phosphate pH 7.4, 30 mM NaCl containing phosphatase inhibitors (PierceTM) and protease inhibitors (Complete, Roche Diagnostics GmbH) and nuclei were eliminated by 900 x g centrifugation ...
-
bioRxiv - Molecular Biology 2023Quote: Whole cell extracts from cultured cells were prepared by direct lysis and sonication of cells in 2% SDS sample buffer containing phosphatase and protease inhibitors (Roche, Germany). Cell extracts were separated in denaturing 8% or 15% (solely used for CDKN1A ...
-
bioRxiv - Cell Biology 2023Quote: ... They were lysed in RIPA buffer (Research products, R26200-250.0) supplemented with protease and phosphatase inhibitor cocktails (Roche, 11836170001 and 4906845001) using a Tissue-Lyser (5 times 5min at 30 shakes/second) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Pieces of tissue were incubated in 500 ml of NBT/BCIP solution (4.5 μl/ml NBT, 3.5 μl/ml BCIP in Alkaline Phosphatase buffer) (Roche 11383213001 and 11383221001) in the dark from some minutes to several hours ...
-
bioRxiv - Molecular Biology 2023Quote: ... and incubated overnight at 4°C in 1% goat serum in MABT with 1∶5000 alkaline phosphatase-coupled anti-Digoxigenin antibody (Roche Diagnostics). On the third day ...
-
bioRxiv - Physiology 2023Quote: ... Cardiomyocyte pellets were lysed in ice-cold 1X RIPA buffer supplemented with 1X protease inhibitors and 1X Phosstop phosphatase inhibitor (Roche #04906837001) and sonicated for 10sec ...
-
bioRxiv - Molecular Biology 2023Quote: ... Antibodies were visualized chromogenically by incubating the membrane in an alkaline phosphatase buffer (130mM Tris, pH 9, 150 mM NaCl, 100 μM MgCl2) and 2% BCIP/NBT (Roche: 11681451001).
-
bioRxiv - Pathology 2023Quote: Cells or tissues were homogenized in RIPA buffer supplemented with protease inhibitors and phosphatase inhibitor cocktails (Roche Diagnostics, Indianapolis, IN, USA). Western blot analysis was performed according to the routine procedure ...
-
bioRxiv - Neuroscience 2023Quote: ... 100 mM TRIS (Fisher BioReagents™) pH 8.5 supplemented with PhosStop phosphatase inhibitor as well as cOmplete™ mini protease inhibitor cocktail (Roche). Samples were subjected to probe-based sonication using a Thermo Sonic Dismembrator and subsequently heated at 95° C for 10 minutes ...
-
bioRxiv - Neuroscience 2023Quote: ... followed incubation with blocking solution for 2 hours before incubation with alkaline phosphatase (AP)-conjugated anti-DIG Fab fragments antibody (1:1000, Roche, #11093274910) overnight at 4°C ...
-
bioRxiv - Plant Biology 2023Quote: ... Meristem-enriched samples or whole seedlings were ground in liquid nitrogen and homogenized in cold protein extraction buffer (50 mM Tris HCl pH 7.5, 10% glycerol, 1 mM DTT, 1% IGEPAL, 1× Roche EDTA-free protease inhibitor and 1× Roche phosphatase inhibitor). The homogenate was centrifuged and the protein concentration of the supernatant was measured using a Pierce 660 nm Protein Assay Reagent (Thermo Scientific) ...
-
bioRxiv - Immunology 2023Quote: ... 1% NP-40, 150 mM NaCl, and 10 mM Tris-HCl [pH 8] supplemented with phosphatase and protease inhibitor cocktail tablets [Roche Diagnostics]). The cell lysates were cleared by centrifugation for 15 min at 40C ...
-
bioRxiv - Cell Biology 2024Quote: The whole lysates of AT or AT-EVs was prepared on ice by using the RIPA lysis buffer (Beyotime, China) containing protease and phosphatase inhibitors (Roche, Germany). After lysis for 20 min ...
-
bioRxiv - Plant Biology 2023Quote: ... 50 mM Tris HCl pH 7.5, 10% glycerol, 10 mM EDTA, 10 mM DTT, 1mM NaF, 1% IGEPAL, 2X protease inhibitor, and 2X Roche phosphatase inhibitor) according to the sample quantity (approximately 200 µl of extraction buffer per 100 mg of ground plant material) ...