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Citations for Roche :
2051 - 2100 of 7357 citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... slides were incubated overnight at room temperature with anti-DIG antibody conjugated with the alkaline phosphatase (1/5,000, Roche Diagnostics) in B1 containing 1% NGS ...
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bioRxiv - Neuroscience 2021Quote: Proteins from brain tissue lysates or primary cortical neurons were extracted using RIPA buffer with protease and phosphatase inhibitor cocktails (Roche). Total lysates were obtained by 30 sec ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was thawed on ice and homogenized using a motorized pestle in RIPA buffer containing both protease and phosphatase inhibitor cocktails (Roche). Lysis was completed for 15 minutes on ice ...
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bioRxiv - Immunology 2021Quote: Cell culture pellets were lysed using RIPA buffer (Pierce, Cat no. 89901) that was supplemented with protease and phosphatase inhibitors (Roche) and sonicated using a Bioruptor UCD-200 (Diagenode) ...
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bioRxiv - Cell Biology 2020Quote: ... protein extracts were prepared as previously described (Alao et al., 2014) with addition of 1 × PhosStop phosphatase inhibitor cocktail (Roche Diagnostics Scandinavia AB ...
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bioRxiv - Immunology 2020Quote: ... 1 mM of EDTA) supplemented with protease and phosphatase inhibitors (EDTA-free protease mixture inhibitor; Roche Diagnostics, Almere, the Netherlands). The protein pellets were homogenized passing through a 27G needle ...
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bioRxiv - Neuroscience 2020Quote: ... and 2 mM EGTA) with Halt phosphatase buffer inhibitor (Fisher: PI78420) and Complete mini EDTA-free protease inhibitor (Roche: 4693159001). Samples were sonicated at low power (Qsonica Q55 ...
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bioRxiv - Neuroscience 2021Quote: ... were either left empty or filled with 6 μl of a mix of post-translational modification (PTM) inhibitors containing the following reagents: phosphatase inhibitors (PhosSTOP, 04906845001, Roche), protease inhibitors (cOmplete Protease Inhibitor Cocktail ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Neuroscience 2019Quote: ... and then incubated overnight at 4°C with sheep anti-DIG alkaline phosphatase conjugated antibody (1:2,000; Roche Applied Sciences). Sections were washed thrice with PTW buffer and twice with coloration buffer (100 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2019Quote: Intestinal tumor and adjacent non-tumor tissue were lysed in KALB lysis buffer supplemented with protease inhibitor and phosphatase inhibitor (Roche) and then homogenized using a QIAGEN TissueLyser II ...
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bioRxiv - Systems Biology 2019Quote: ... 1 mM EDTA) supplemented with phosphatase inhibitors (5 mM b-glycerophosphate, 5 mM NaF, 1 mM Na3VO4 and protease inhibitors (Roche cOmplete ULTRA Tablets ...
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bioRxiv - Neuroscience 2019Quote: ... Neurons were washed three times with PBS and harvested directly in 8 M Urea lysis buffer supplemented with phosphatase inhibitor (PhosSTOP, Roche) and protease inhibitor (cOmplete mini EDTA-free ...
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bioRxiv - Developmental Biology 2019Quote: Three to five-day-old female ovaries were dissected and homogenized in RIPA lysis buffer containing protease and phosphatase inhibitors (Roche). In some samples ...
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bioRxiv - Cancer Biology 2021Quote: Lysates were prepared from tumor tissue or cultured cells using RIPA buffer supplemented with fresh protease and phosphatase inhibitors (Roche). Protein quantification was performed using the BCA Protein Assay (G Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% sheep serum in PBST) for one hour and overnight at 4 °C with alkaline phosphatase-conjugated anti-DIG antibodies (Roche) diluted at 1:4000 in the blocking solution ...
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bioRxiv - Systems Biology 2020Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Cell Biology 2019Quote: ... 11 836 145 001), and phosphatase (PhosphoSTOP, Cat. No. 04 906 837 001)) inhibitor cocktail tablets were purchased from Roche Applied Science ...
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bioRxiv - Developmental Biology 2019Quote: ... treatments to remove the first antibody (42) followed by overnight incubation of pooled samples with anti-FLU antibody conjugated to alkaline phosphatase (1:2000, Roche). Expression of myl7 in 28 and 55 hpf embryos was then revealed by Fast Red (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5 % sodium deoxycholate and 1 % Triton X-100 as well as protease inhibitors (10 mg / ml leupeptin, pepstatin A, 4-(2-aminoethyl) benzensulfonyl-fluorid and aprotinin) and phosphatase inhibitors (PhosSTOP−, Roche). Total cell lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Physiology 2020Quote: ... Sections were then dried and covered in an alkaline phosphatase (AP)-conjugated anti-DIG antibody (1:3000; Roche, Mannheim, Germany) in buffer B1/2.5% goat serum at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... Protein aggregation was evaluated by western blot in total cell extracts prepared in 1% Triton X-100 in PBS containing proteases and phosphatases inhibitors (Roche). Sample quantification was performed with the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Genetics 2021Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP-40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)) for 10 minutes at 4°C and spun at 1350g for 5 minutes at 4°C to isolate nuclei ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Neuroscience 2021Quote: Dissected brain regions of interest or culture samples were homogenized with TX-soluble buffer (50 mM Tris [pH 8.0], 150 mM NaCl, 1% Triton-X 100) containing protease and phosphatase inhibitors (Roche, USA). The supernatants were collected for soluble fraction after centrifugation (20 ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 mM NaF) or AMPK lysis buffer (Faubert et al., 2013) supplemented with the following protease additives: protease and phosphatase tablets (Roche), DTT (1 μg/mL) ...
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bioRxiv - Developmental Biology 2021Quote: ... brn3a1 and brn3a2(l) transcripts were visualized with anti digoxigenin-alkaline phosphatase conjugates and BCIP/NBT or BM purple substrates (Roche). For two color WISH krx-20 (Oxytoby and Jowett ...
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bioRxiv - Cell Biology 2020Quote: ... followed by addition of 300 μL of RIPA buffer containing cOmplete™ protease inhibitor and phosphatase inhibitor (both from Roche). Cells were scraped with cell scrapers and transferred to 1.5 tubes ...
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bioRxiv - Cell Biology 2019Quote: Protein lysates from whole pancreas were prepared in 1 mL of RIPA buffer supplemented with phosSTOP phosphatase and protease inhibitors (Roche). Protein concentrations were measured using the BCA kit (Thermo Scientific ...
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bioRxiv - Genomics 2020Quote: ... Sections were then washed several times with SSC of increasing stringency and then incubated with alkaline phosphatase-conjugated anti-digoxigenin antibodies (Roche). Nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Whole cell lysates were obtained by directly exposing cells to sodium dodecyl sulphate (SDS) lysis buffer supplemented with phosphatase and protease inhibitors (Roche). Membranes were incubated in Roti block (Roth ...
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bioRxiv - Immunology 2021Quote: Fresh cell samples were lysed in RIPA buffer (Thermo) supplemented with complete EDTA-free Protease inhibitor cocktail and phosphatase inhibitors (Roche) and sonicated on a Bioruptor (Diagenode) ...
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bioRxiv - Molecular Biology 2021Quote: ... Semi-confluent astrocyte cultures were lysed in ice cold radio-immunoprecipitation assay lysis buffer with protease and phosphatase inhibitors (Roche), and 10 µg protein was separated by electrophoresis on 4-12% polyacrylamide gels followed by transfer to nitrocellulose membranes (all gels/reagents from Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: BMDMs and THP-1 macrophage-like cells were lysed in ice-cold RIPA buffer supplemented with complete protease inhibitor and phosphatase inhibitor cocktails (Roche). Protein concentration was measured by the Pierce™ BCA Protein Assay Kit (Thermo Scientific-23227) ...
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bioRxiv - Immunology 2021Quote: ... HEK293T and A549 cells were lysed in ice-cold RIPA lysis buffer containing complete protease inhibitor and phosphatase inhibitor cocktails (Roche), resolved by SDS-PAGE ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) and 5% (v/v) glycerol and Supplementarylemented with a protease inhibitor cocktail tablet and a phosphatase inhibitor tablet (Roche). Lysates were briefly sonicated on ice and clarified by centrifuged at 20,817g for 20 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... N’-tetraacetic acid (EGTA) and 5% (v/v) glycerol and supplemented with a protease inhibitor cocktail tablet and a phosphatase inhibitor tablet (Roche). Lysates were briefly sonicated on ice and clarified by centrifuged at 20,817g for 20 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: Western blot lysates were produced in RIPA buffer (Pierce #89900) supplemented with cOmplete Mini EDTA-free protease inhibitor cocktail and PhoshoStop phosphatase inhibitor tabs (Roche) and 1 mM DTT at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice in cold 1× PBS and harvested in 2% SDS in 50 mM HEPES buffer with freshly added protease and phosphatase inhibitors (Roche). Cell lysates were sonicated and centrifuged for 15 min at 12000 ×g to remove the insoluble fraction ...
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bioRxiv - Immunology 2022Quote: ... 90 μl of faecal suspension was mixed with 10 μl (10x) buffer and 0.5 μl (0.5 U) calf intestinal phosphatase (APMB-RO, Roche, Sigma-Aldrich) and incubated at 37 °C for 60 min ...
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bioRxiv - Physiology 2022Quote: ... the sections were blocked in TS7.5 containing 1% Blocking Reagent for 1 hr and then incubated with alkaline phosphatase-conjugated anti-digoxigenin sheep antibody (1:1000; 11093274910, Roche) and anti-EP3R rabbit antibody in this blocking solution overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were hybridised with monoclonal antibodies against GFP followed by anti-mouse alkaline-phosphatase conjugated antibody and signals were developed using NBT-BCIP (Roche).
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bioRxiv - Neuroscience 2022Quote: ... eIF2αS/S and eIF2αA/A MEFs transduced with TauRD-YFP and Biosensor cell transfected with wild-type and PS19 brain lysates were lysed with SDS lysis buffer (2% SDS in PBS containing protease and phosphatase inhibitors (11836153001, Roche)) or RIPA buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... Hybridization signals were detected with anti-mouse secondary antibody labeled with alkaline phosphatase using NBT/BCIP substrates (Roche Biochemical, Mannheim).
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bioRxiv - Biochemistry 2022Quote: ... Cells were scraped with a spatula and lysed in RIPA buffer supplemented with protease and phosphatase inhibitors (cOmplete and phosSTOP, Roche) on ice for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... cells were lysed in Western Blot (WB) lysis buffer (1% SDS, 10 mM Tris, 1 mM EDTA, phosphatase and protease inhibitors (Roche, 04906837001 and 04693159001 ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was homogenized in a ‘homogenization buffer’ (Sucrose, 100 mM EGTA, 10 mM Tris, protease and phosphatase inhibitors (Roche), pH 8.1 ...
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bioRxiv - Biochemistry 2022Quote: Cells were washed and harvested in PBS 1x, lysed in RIPA buffer 1x (Thermo, 88900) containing phosphatase and protease inhibitors (Roche). Lysates were centrifuged at 15,000 g to separate soluble and pellet fractions ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were washed with high stringency (0.5× SSC, 70°C) and developed with alkaline phosphatase-conjugated anti-DIG antibody (Roche, Switzerland) with CDP-Star substrate in accordance with the manufacturer’s instructions ...