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Citations for Roche :
1 - 50 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 0.1% SDS with protease inhibitor cocktail (Roche, 04693132001). Worms were lysed with 1% Nonidet P-40 worm lysis buffer (40 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... Seven-micron sections were then stained with TUNEL according to manufactures protocol (Roche 11684795910 ...
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bioRxiv - Cell Biology 2024Quote: ... they were digested in 1.5 mg/ml Collagenase/Dispase (Roche, 11097113001) dissolved in DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... cultured cells were washed with PBS and lysed in ice-cold RIPA buffer supplemented with EDTA-free cOMPLETE protease inhibitor (Roche) on ice ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were permeabilised by 10 minutes incubation with 0.1% Triton X-100 on ice and incubated with blocking buffer (10% FBS in 1X PBS) for 90 minutes and then probed with the primary antibody (anti-HA rat 1:100 (Roche) for 2 hours on rotor and after three washing with 1x PBS cells were probed with anti-rat Alex Flour-488 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: The whole lysates of AT or AT-EVs was prepared on ice by using the RIPA lysis buffer (Beyotime, China) containing protease and phosphatase inhibitors (Roche, Germany). After lysis for 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were incubated with blocking solution (5% skimmed milk in 1X PBS) followed by overnight incubation with respective primary antibodies (anti HA rat 1:1000 (Roche), anti BiP rabbit 1:5000 in cold room and 1 hr incubation with respective secondary antibodies (1:10000 ...
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bioRxiv - Cell Biology 2024Quote: ... 2 ng of cDNA were amplified per reaction and each reaction was performed in triplicate using the LightCycler 384 (Roche) with SYBR Green Master Mix II (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... with SYBR Green Master Mix II (Roche). A melting curve was performed and analyzed for each gene to ensure the specificity of the amplification ...
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bioRxiv - Cell Biology 2024Quote: ... and detected by immunoblotting with polyclonal anti-HA (Roche, RRID:AB_514506) or anti-GFP antiserum (Living Colors ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by staining with 50x NBT/BCIP (Roche) in buffer B2 for 2-4 hr ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.15M NaCl) for 1 hour at R/T and then incubated with anti-DIG antibody (Roche; 1:5000) overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin-labeled riboprobes were transcribed using the DIG-RNA Labeling Mix (Roche). Embryonic heads (E11.5–E13.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... supplemented with Complete Mini EDTA-free protease inhibitor cocktail (Roche, 04693159001) and PhosSTOP (phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... and PhosSTOP (phosphatase inhibitor cocktail (Roche, 04906845001). Protein extraction was performed by incubating the samples on ice for 15 minutes and then collecting the supernatant after centrifugation at 13,000 rpm at 4°C for 30 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... BM Purple AP Substrate precipitating Solution (Roche, Mannheim, Germany) was used to visualize the digoxigenin-labelled riboprobes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Library quality was assessed with an Agilent 4200 Tapestation and Lightcycler quantitative PCR (Roche Life Science, St. Louis, MO) and then paired-end sequenced (2 × 150 bp ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Extracted DNA samples were sent to Admera Health (South Plainfield, NJ) and were prepared using the KAPA HyperPrep kit (Kapa Biosystems, Inc., Wilmington, MD) according to the provided instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and amplified by PCR with HiFi Kapa Hot start ReadyMix (Kapa Biosystems, KK2601). After clean-up with SPRI beads ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA was extracted from 200 µL of indicated cell supernatants using a High Pure Viral RNA Kit (Roche, Germany). The extracted RNA was eluted in 50 µL of elution buffer ...
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bioRxiv - Cell Biology 2024Quote: ... before transfer to polyvinylidene fluoride (PVDF) membranes (IPVH00010, Roche Diagnostics, China) using standard procedures ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with protease inhibitor cocktail (Rowe Scientific; CP2778) and PhosSTOP EASYpack Phosphatase Inhibitor Cocktail (Roche; 4906837001), and kept on ice for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... and from WT and Hcar1 KO mice were lysed in RIPA buffer (cell signaling) supplemented with 0.1 mg/ml PMSF and proteinase inhibitor cocktail (11697498001, Roche, USA), and then were homogenized thoroughly (BRANSON Sonifier 150 ...
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bioRxiv - Cell Biology 2024Quote: ... and then analyzed by qPCR with FastStart SYBR Green Master Mix (Roche) using a specific primer set for each gene (Supplementary Table X) ...
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bioRxiv - Cell Biology 2024Quote: ... 1X complete protease inhibitor cocktail (Roche, Cat. # 04693132001), 1 mM sodium orthovanadate (Na3VO4 ...
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bioRxiv - Cell Biology 2024Quote: ... and EDTA-free protease inhibitor cocktail (Roche) and lysates spun at 4 °C at 10,000 x g for 10 min to yield supernatants for analysis ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitation of GFP/YFP-tagged proteins was performed with anti-GFP antibodies (11814460001, Roche) using a method we previously described (Ishii and Akiyoshi ...
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bioRxiv - Cell Biology 2024Quote: ... Cell growth was monitored using a CASY cell counter (Roche). Expression of GFP fusion proteins and RNAi were induced with doxycycline at a final concentration of 10 ng/mL and 1 µg/mL ...
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bioRxiv - Cell Biology 2024Quote: Cell viability staining was performed using the In situ cell death detection kit (Roche Diagnostic). In summary ...
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bioRxiv - Cell Biology 2024Quote: ... and phospho-stop (Roche). Spheroids were sonicated three times with an ultrasonic processor (UP50H ...
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bioRxiv - Cell Biology 2024Quote: ... 10 mM NaF and 5 mM Na3VO4) containing protease/phosphatase inhibitors (Roche). Cells were incubated in lysis buffer for 2-3 hours in rotator at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and protease inhibitors (Roche, #05 892 970 001) for four hours at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and EDTA-free protease inhibitor cocktail (Roche). Protein concentration was measured using the Bradford method (Bio-Rad # 500-0006) ...
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bioRxiv - Cell Biology 2024Quote: ... EDTA-free Protease Inhibitor Cocktail Tablet was from Roche (catalog no. 11836170001). Protein A/G PLUS-Agarose (Catalog no ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated using the High Pure RNA Isolation kit (#11828665001, Roche, Switzerland), followed by cDNA synthesis with the Maxima First Strand cDNA synthesis kit ((#A25741 ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 2.4 U/ml dispase II (#4942078001, Roche, Switzerland), 2 mg/ml collagenase A (#1013586001 ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was isolated using the High Pure RNA Isolation kit (Roche, Switzerland).
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bioRxiv - Cell Biology 2024Quote: ... cells were washed twice with PBS and lysed for 20 min on ice in lysis buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40, supplemented with protease inhibitors [Roche]). Centrifugation at 13,000×g for 10 min at 4°C was then used to remove cell debris ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.1 mg/ml DNase I (#1014159001, Roche, Switzerland). The samples were resuspended in ice-cold Hanks balanced salt solution (HBSS ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mg/ml collagenase A (#1013586001, Roche, Switzerland), 0.2 mM CaCl2 (#C5670 ...
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bioRxiv - Cell Biology 2024Quote: ... PhosSTOP (Roche, 4906845001) and 10 mM NEM (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... PhosSTOP (Roche, 49068450001) and 20 mM NEM (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... and PhosSTOP (Roche, 49068450001) for 10 mins on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.05 mg/ml DNase1 (Roche Diagnostics Gmbh) dissolved in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Developmental Biology 2024Quote: ... TUNEL (In Situ Cell Death Detection Kit, TMR Red, Sigma/Roche); anti-Kmg (Fuller lab).
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bioRxiv - Developmental Biology 2024Quote: ... Per sample 200 ng of total RNA was used for library preparations with the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Kapa Biosystems), including steps of the oligonucleotide hybridization ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 U/µL Protector RNase inhibitor (Roche, 3335399001) in Nuclease-free water ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were generated with the RiboErase depletion RNA-sequencing protocol by KAPA (Roche, 8098131702) and sequenced on the Illumina Next Seq 2000 ...
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bioRxiv - Developmental Biology 2024Quote: ... and in situ hybridization probes (Roche DIG RNA Labeling Mix and T7 RNA Polymerase kits) ...
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bioRxiv - Cell Biology 2024Quote: ... ChIP-qPCR was performed on a LightCycler 480 instrument (Roche) using SYBR Green I Master Mix (4887352001 ...