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Citations for Roche :
1701 - 1750 of 7357 citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 10% glycerol and 1% Triton X-100) supplemented with a protease and phosphatase inhibitor mixture (Roche) for 20min on ice ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with phosphatase and protease inhibitors (PhosStop and cOmplete Mini, EDTA- free Protease inhibitor Cocktail, Roche). Frozen cell samples were homogenized by using a 26G needle (5 times ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5% w/v SDS and 90 mM NaCl] supplemented with 1x Protease & Phosphatase Inhibitor Cocktail (Roche) and 100 U/mL Denarase (c-LEcta) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed in lysis buffer supplemented with 1x phosphatase inhibitors (PhosStop, Roche Diagnostics, Basel, Switzerland) and a 1x protease inhibitor cocktail (Complete Mini ...
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bioRxiv - Microbiology 2022Quote: ... and 1% NP-40) supplemented with cOmplete protease inhibitor cocktail and PhosSTOP phosphatase inhibitor cocktail (Roche). After centrifugation at 20,000 × g for 15 min at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Protease inhibitors (PI, cOmplete, #4693116001) and phosphatase inhibitors (PhI, PhosSTOP, #4906837001) were from Roche (Mannheim, Germany). Recombinant murine IFNβ (#12405-1 ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were lysed on ice in phosphate buffered saline (PBS) containing phosphatase (PhosphoSTOP Tablet, Roche) and protease (Complete Mini Tablets ...
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bioRxiv - Microbiology 2023Quote: Tissues were homogenized in RIPA lysis buffer (Abcam ab156034, supplemented with phosphatase inhibitor cocktail, Roche 04906845001) for protein extraction ...
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bioRxiv - Developmental Biology 2023Quote: ... Detection was done with alkaline phosphatase-labeled anti-DIG antibody and NBT/BCIP (Roche diagnostics, USA). Control experiments with sense probes did not give detectable signals (data not shown) ...
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bioRxiv - Biochemistry 2023Quote: ... 10 mM MgCl2 and 20% (v/v) glycerol and supplemented with protease and phosphatase inhibitors (Roche). Lysates were sonicated briefly on ice and clarified by centrifuged at 20,817g for 20 min at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysates were extracted with ice-cold RIPA buffer supplemented with protease and phosphatase inhibitors (Roche). Thirty μg of protein lysates were separated using SDS–polyacrylamide gel electrophoresis and transferred to a PVDF membrane (Immobilion-P ...
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bioRxiv - Developmental Biology 2023Quote: ... Each sample was lyzed in 200 mM HEPES buffer (pH 8) containing PhosSTOP phosphatase inhibitors (Roche), phosphatase inhibitor cocktail2 (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... DIG was detected with an anti-DIG antibody conjugated to alkaline phosphatase (RRID: AB_514497; Roche Diagnostics) and visualized with Fast Red (Roche Diagnostics) ...
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bioRxiv - Developmental Biology 2023Quote: ... Each RNA probe was visualized by exposure to an anti-digoxigenin-alkaline phosphatase conjugate antibody (Roche) prior to exposure to a color reaction solution containing NBT/BCIP (nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolyphosphate ...
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bioRxiv - Cell Biology 2023Quote: Cells for immunoblots were lysed in ice cold RIPA buffer with protease and phosphatase inhibitors (Roche). Lysates were quantified using a DC protein assay (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... at room temperature for 1 h and incubated with alkaline phosphatase-conjugated anti-DIG antibody (Roche) overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated with a 1:5000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche 11093274910) in MABT/Block overnight at 4°C ...
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bioRxiv - Physiology 2024Quote: ... Hybridized probes were detected using an alkaline phosphatase-conjugated anti-DIG antibody (RRID: AB_514497; Roche Diagnostics) with nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP ...
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bioRxiv - Physiology 2024Quote: ... and incubated overnight with the alkaline phosphatase (AP) coupled anti-digoxigenin or anti-fluorescein antibody (Roche) at respectively 1/5000 or 1/1000 in blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were incubated overnight at 4°C in alkaline phosphatase-coupled anti-FITC antiserum (11426338910, Roche) diluted 1:5000 in blocking buffer ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with protease inhibitor cocktail (Rowe Scientific; CP2778) and PhosSTOP EASYpack Phosphatase Inhibitor Cocktail (Roche; 4906837001), and kept on ice for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... and 1.0% Nonidet P-40) containing protease and phosphatase inhibitors (PMSF, NaF, Na3VO4, and Roche PhosSTOP). Lysates were cleared by centrifuging at 10,000 g for 10 min at 4° C ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates from human RMS cell lines and human myoblasts were obtained following lysis in RIPA lysis buffer supplemented with protease inhibitors (Roche). Western blot analysis was performed similar to Ignatius et ...
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bioRxiv - Developmental Biology 2021Quote: ... The DIG-labeled 3’UTR of Emi2 and cyclin B1 RNA probes were synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals). Two μg of probes was incubated with purified Flag-tagged proteins for 15 min ...
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bioRxiv - Genetics 2021Quote: ... The T7 promoter was added to the 5’ and 3’ ends to synthesize the sense and anti-sense probes using the DIG RNA Labeling Kit (Roche, Cat# 11175025910). In situ hybridization was performed as previously described [65,66] with minor modifications ...
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bioRxiv - Microbiology 2020Quote: ... and prepared for shotgun metagenomic sequencing using the Kapa Hyperprep Plus kit with 3 rounds of PCR amplification (Kapa Biosystems, Wilmington, MA), and sequenced to an average depth of 7 gbp per fraction on the Illumina NovaSeq (Illumina ...
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bioRxiv - Microbiology 2023Quote: Transcriptional start sites (TSS) of rokL6 and sco1448 were determined by 5’ RACE using a 5’/3’ RACE Kit 2nd generation (Roche, California, US). Total RNA (2 μg ...
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bioRxiv - Genomics 2021Quote: ... rp49 5’-TAATACGACTCACTATAGGGCAGTAAACGCGGTTCTGCATG-3’ and 5’-CAGCATACAGGCCCAAGATC-3’) were transcribed using the Biotin RNA Labeling Mix (Roche) and T7 polymerase (Promega).
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bioRxiv - Biophysics 2019Quote: ... We employed fibronectin (FN, from human plasma, Roche Applied Science ...
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bioRxiv - Neuroscience 2022Quote: ... The media for human NPCs obtained from Roche was supplemented with B-27-supplement minus vitamin A (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... 10 ng/ml recombinant human EGF (Roche, Ireland), 1 μg/ml hydrocortisone (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... human NEMO (5µg) and ATP (2mM) (Roche, 1051997900) were incubated in a reaction buffer consisting of 50mM HEPES (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Human IFN-α2a (Roferon) was purchased from Roche. 2’-3’-cGAMP and c-di-UMP were purchased from Invivogen ...
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bioRxiv - Genomics 2022Quote: ... 100 ng of human genomic DNA (11691112001, Roche) was tagmented in 25 μl reactions by adding 12.5 μl of 2X tagmentation buffer (20mM Tris-HCl pH 7.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... Human gDNA of high molecular weight (Roche Diagnostics) and ssDNA (M13mp18 single-stranded virion DNA ...
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bioRxiv - Genomics 2019Quote: ... trachomatis molecular diagnosis was performed by using a dual-target (chromosome plus plasmid) commercial nucleic acid amplification test (NAAT) (Cobas® 4800 CT/NG from Roche Diagnostics) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Adapter ligation was performed for 1 hour at 20°C using the Kapa Unique-Dual Indexed Adapters diluted to 1.5 μM concentration (Roche Sequencing Solutions Inc.). After ligation ...
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bioRxiv - Biochemistry 2021Quote: ... 5mM CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) and 1 tablet of Protease Inhibitor Cocktail (ROCHE, cOmplete™). The preparation was sonicated for 120 s on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... Whole cell extracts were prepared by lysis in RIPA buffer supplemented with protease and phosphatase inhibitors (Roche). Protein concentration was quantified by BCA assay (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... All lysates included protease and phosphatase inhibitors at the recommended concentration (Complete-EDTA-free and PhosSTOP [Roche]).
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bioRxiv - Developmental Biology 2021Quote: ... Signals of transcripts were detected using Anti-DIG-AP (alkaline phosphatase) and Fab fragments from sheep (Roche) and developed in Fast Red TR and naphthol-AS-MX-phosphate in 0.1 M Tris-HCl pH 8.2 (tablet set ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were incubated overnight with anti-DIG antibody conjugated to alkaline phosphatase (1:5000) (no. 11093274910, Roche) at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Tissues were lysed with 8 M urea in PBS supplemented with protease and phosphatase inhibitor cocktail (Roche) at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... 1% Triton X-100 plus protease and phosphatase inhibitor cocktails (Complete Mini, EDTA-free; PhosSTOP; Roche Diagnostics). Insoluble material was cleared by centrifugation for 10 min at 20,000xg ...
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bioRxiv - Cell Biology 2019Quote: ... signals were detected using anti-DIG labeled alkaline phosphatase antibody by chromogen method using NBT/BCIP (Roche). Sections were counter stained with nuclear fast red and mounted after dehydration ...
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bioRxiv - Developmental Biology 2019Quote: ... the signals were visualized according to their immunoreactivity with anti-digoxigenin alkaline phosphatase-conjugated Fab fragments (Roche). At least three embryos of each genotype were used for each analysis.
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bioRxiv - Plant Biology 2020Quote: ... PhosSTOP™ phosphatase inhibitor and Complete™ EDTA-free protease inhibitor coctail (both from Roche, Basel, Switzerland)) and the extract was centrifuged (13 000 g ...
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bioRxiv - Systems Biology 2019Quote: ... pH 7.4) containing protease and phosphatase inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche and PhosSTOP, Roche) with a ratio of 1 volume packed RBC ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Purple/dark blue staining was detected using alkaline phosphatase-conjugated anti-DIG antibody FAB fragments (Roche Diagnostics) and Nitro Blue tetrazolium/5-bromo-4-chloro-3-indolyl-phosphate NBT/BCIP (Roche Diagnostics) ...
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bioRxiv - Microbiology 2021Quote: Cells were washed in PBS and lysed in NTEN buffer supplemented with phosphatase and protease inhibitors (Roche). Protein concentrations were determined using a BCA assay (Pierce) ...