Citations for Lucigen :
1 - 50 of 1933
citations
since 2017
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... are listed in Table S3 and RNA extraction was carried out using Master Pure yeast RNA extraction purification kit (Epicentre) from budding cells and cells incubated with serum ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmid-safe ATP-dependent DNase was obtained from Lucigen, WI ...
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bioRxiv - Microbiology 2022Quote: The RNAs from Mucor strains were extracted using the MasterPure™ yeast RNA purification kit (Lucigen MPY03100). Extracted RNA was then converted to cDNA by utilizing a high-capacity cDNA reverse transcription kit (Applied Biosystems™ 4374966) ...
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bioRxiv - Molecular Biology 2022Quote: ... The linearized plasmids were used as template to generated mRNA by in vitro transcription using the AmpliScribe SP6 Transcription Kit (Epicentre AS3106), followed by capping using the ScriptCap m7G Capping System (Cellscript C-SCCE0625) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was precipitated using ispopropanol and contaminating RNA removed using RiboShredder™ RNase Blend (Epicentre). RNase was removed using an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1) ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was extracted using the Masterpure Complete DNA Purification Kit (Lucigen-Biosearch Technologies ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA from all smut species was isolated using a MasterPure™ Complete DNA&RNA Purification Kit (Epicentre®, Illumina®, Madison, Wisconsin, USA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... coli cells (Lucigen cat. no. 60242-1) using prechilled 1 mm electroporation cuvettes (BioRad cat ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNAs were prepared using QuickExtract DNA Extraction Solution (Lucigen) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... DNA-Seq library preparation and sequencing were performed by the Genome Quebec Company (Genome Quebec Innovation Center, McGill University, Montreal, Canada) using the Shotgun PCR-free library preparation kit (Lucigen) and the NovaSeq™ 6000 Sequencing system (Illumina® ...
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bioRxiv - Systems Biology 2022Quote: ... the ribosomal RNA was depleted by Epicentre Ribo-zero® rRNA Removal Kit (Epicentre). Second ...
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bioRxiv - Systems Biology 2022Quote: ... the ribosomal RNA was depleted by Epicentre Ribo-zero® rRNA Removal Kit (Epicentre) ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (Lucigen) and purified as described by Urbanek et al ...
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bioRxiv - Biochemistry 2022Quote: ... coli C43(DE3) (Lucigen). E ...
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bioRxiv - Biochemistry 2022Quote: ... Cloni 10G electrocompentent cells (Lucigen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic DNA of expanded clones was isolated using QuickExtract DNA extraction solution (Lucigen, QE0905T). PCR was performed by amplifying a 241bp area surrounding the mutation site ...
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bioRxiv - Developmental Biology 2022Quote: ... genomic DNA from each colony was harvested with QuickExtract DNA Extraction (Lucigen, QE09050) and then used for PCR amplification ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cloni 10G SOLO cells (Lucigen). Whole transformations were plated on LB-agar + 25 μg/mL chloramphenicol and grown overnight ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cloni 10G cells (Lucigen, USA). Cells were grown overnight at 37 °C on LB-agar + 50 μg/mL kanamycin plates and transformants scraped for plasmid DNA isolation ...
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bioRxiv - Cell Biology 2022Quote: ... and 5 U RecJ exonuclease (Epicentre). Up to 8 libraries were pooled ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were treated with 500 U of RNAse I (Epicentre) per 25 μg of RNA (quantified by Qubit fluorimetry – ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... At this stage a small aliquot of cells (75% of the plate) was lysed in 200 μL of QuickExtract DNA Extraction solution (Lucigen) and gDNA was prepared by heating to 65° C for 10 minutes followed by heat inactivation at 95° C for 2 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... cDNAs were circularized using 100U CircLigase I ssDNA ligase (Epicentre), and cDNA concentration was quantified by QPCR of cDNA compared to a standard curve of a reference sample ...
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bioRxiv - Cell Biology 2022Quote: ... coli 10G SUPREME electrocompetent cells (Lucigen). This plasmid pool was used to produce lentivirus-containing supernatants in HEK293T cells ...
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bioRxiv - Immunology 2022Quote: ... coli C41 OverExpress™ C41(DE3) (Lucigen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and additionally purified with a mRNA-ONLY™ Eukaryotic mRNA Isolation Kit (Epicentre). Transcriptome libraries were then constructed using SOLiD total RNA-seq kit (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2022Quote: ... or Epi400 (Lucigen). Standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cloning was carried out by adding the two 15kb fragments (80fmol each) to 40fmol pSMART BAC vector (Lucigen) and incubating them with a proprietary assembly mix (Twist ...
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bioRxiv - Synthetic Biology 2022Quote: ... Transposed products were enriched by electroporating approximately 100 ng of plasmid pool into Endura Electrocompetent Cells (Lucigen), which are a non- PIR strain that limits recombination ...
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bioRxiv - Synthetic Biology 2022Quote: ... cloni EXPRESS BL21 (DE3) cells (Lucigen) were transformed with each library and expressed in a 96-well deep well format.21 General methods for liquid handling and fluorescence plate reader screening at pH 5 ...
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bioRxiv - Plant Biology 2022Quote: Five micrograms of RNA isolated from enriched chloroplasts was digested with 6U of Terminator exonuclease (Lucigen catalog number TER51020) for 1h at 30°C in 20µl reaction volume ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... genomic DNA from single colonies was extracted with the MasterPureTM DNA Purification Kit from Epicentre (Cat. No. MCD85201) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... two µg of total RNA were treated with 5 U of RNase R (cat. # RNR07250, Lucigen, Middleton, WI) for 40 min at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... The resulting air-dried pellets were resuspended in RNase-free water and 3 µg of DNA from each sample was depleted of ribosomal RNA using the Bacteria Ribo-Zero rRNA Removal Kit (Epicentre) and purified using the RNeasy miniprep kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... Clonal populations were genotyped by Sanger sequencing the PCR amplified sgRNA-targeted sites in the gDNA extracted using DNA QuickExtract (Lucigen). Resulting sequences were compared to references and clones containing a frameshift indel or de novo stop codon were selected ...
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bioRxiv - Developmental Biology 2022Quote: ... Single cells were FACS sorted into 1 ul each of QuickExtract DNA Extraction Buffer (Lucigen). DNA was extracted by incubating cells for 10 minutes at 65°C followed by 5 minutes at 98°C ...
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bioRxiv - Cell Biology 2022Quote: ... The homology arms were amplified from extracted HEK-293 cell genomic DNA (QuickExtract DNA extraction solution, Lucigen). The fidelity of all constructs was confirmed by Sanger sequencing prior to use.
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bioRxiv - Neuroscience 2022Quote: Monophosphorylated RNAs were selectively degraded by Terminator 5’-phosphate-dependent exonuclease (Lucigen). Subsequent 5’ dephosphorylation by quickCIP (NEB ...
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bioRxiv - Bioengineering 2022Quote: 2’-Fluoro ssRNA was produced by IVT using the Durascribe T7 IVT kit (Epicentre). Reactions consisted of 0.2-1 μg of purified DNA template per 20 μl reaction ...
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bioRxiv - Synthetic Biology 2022Quote: ... the DNA library was transformed into TG1 electrocompetent cells (Lucigen) with an efficiency of approximately 1010 CFU per library before amplification ...
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bioRxiv - Genomics 2022Quote: Equal amounts of RNA (2µg) were incubated with or without 20 U of Rnase R (RNR07250, Epicentre Biotechnologies) and 40 U of RiboLock RNase inhibitor (EO0381,Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... rRNA depleted islet RNA was digested using RNase R (RNR07250, Lucigen) or mock digestion (protocol without RNase R ...
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bioRxiv - Microbiology 2022Quote: ... coli were induced to express pAZ001 using CopyControl Induction Solution (Lucigen, Middleton, WI, USA). When necessary ...
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bioRxiv - Microbiology 2022Quote: ... coli (Lucigen, Middleton, WI, USA), and plated on LB with chloramphenicol for selection ...
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bioRxiv - Microbiology 2022Quote: ... high molecular weight DNA was isolated using the MasterPure DNA Purification kit (Epicentre, no. MC85200). Sequencing was performed using the PacBio Sequel (Pacific Biosciences ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted using the MasterPure kit (Lucigen, cat no MC85200).
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bioRxiv - Microbiology 2022Quote: ... The ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) was used to make the libraries according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using MasterPure Yeast DNA purification Kit (Epicentre) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... The oligo pools were cloned into a modified pLKO-TET-ON plasmid by the Golden Gate method and the resulting product was used to transform Endura electrocompetent cells (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (Lucigen). To allow for fluorescent labeling of Wsp1p-PRD ...