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Citations for Polysciences :
301 - 350 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... 8.3 µg psPAX and 2.5 µg pMD2.G were mixed with 80 µg PEI MAX 40K (Polysciences, 24765–1) in 1.5 ml of Expi293 media ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.5 mM spermidine and 1× protease inhibitor) with activated Concanavalin A Magnetic Beads (Polysciences, 86057-3) for 10 mins at RT ...
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bioRxiv - Biochemistry 2024Quote: ... was expressed with a TEV protease cleavable N-terminal 6xHis-tag and IgK secretion signal in HEK293 GnTI-cells upon transient transfection with PEI MAX (Polysciences Inc., 24765). The IgK secretion signal allowed for the secretion of LyLAP recombinant protein ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293 and HEK293 β-arrestin1/2 KO cells were transfected in suspension with a total of 1 μg DNA and 6 μg 25 kDa linear polyethyleimine (PEI, Polysciences Inc.) in 150 mM NaCl solution per 1 million cells ...
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bioRxiv - Neuroscience 2024Quote: ... Cryosections were mounted for microscopy on glass slides using Aqua-Poly/Mount (Polysciences, 18606) or Vectashield (Vector labs ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were added 900 µg of linear polyethylenimine 25 kDa (Polysciences) and 3 µmol of kifunensine per 100 mL of the medium and continued incubation ...
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bioRxiv - Biochemistry 2024Quote: ... 1.1 mg of a 1:1 (w/w) mixture of the GABAAR α1 subunit and NACHO DNA and 3 mg of Polyethylenimine “Max” (Polysciences #24765) were dissolved separately in 30 mL Expi293 expression media ...
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bioRxiv - Biochemistry 2024Quote: ... while Huh7 cells were reversibly transfected as described previously.48 DNA was transfected using PEI (polyethylenimine, Polysciences Inc., Cat# 23966). The transfection mixture included 200 µL DMEM without supplements ...
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bioRxiv - Microbiology 2024Quote: ... a Tat-encoding plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1:3 ratio using polyethyleneimine (PEI, Polysciences). After 8 hours ...
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bioRxiv - Microbiology 2024Quote: ... freshly seeded 293T cells were grown in DMEM supplemented with 10% fetal bovine serum (FBS) and 50 µg/mL gentamicin at 37°C with 5% CO2 and transfected using polyethylenimine (Polysciences) [34] ...
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bioRxiv - Microbiology 2024Quote: ... mixed with 24 µL PEI (Polysciences) in a final volume of 600 µL and incubated for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were hydrated by washing three times with PBS before incubating in LAB buffer (PolySciences Ltd) for 5 minutes at room temperature for a gentle antigen retrieval ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then dehydrated in acetone and embedded in Polybed 812 resin (Polysciences). Ultrathin (60 nm ...
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bioRxiv - Microbiology 2024Quote: ... Cells were transfected with plasmids of interest (Table 3) using polyethylenimine (PEI, Polysciences). At 4 h post-transfection ...
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bioRxiv - Microbiology 2024Quote: ... using polyethylenimine (PEI, Polysciences). 4 h post-transfection ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C in a 5% CO2 incubator for 48 hours prior to fixation with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells (2 × 106 cells on a 10 cm dish) were transfected with 12 μg of plasmid DNA and 36 μg of polyethylenimine hydrochloride (PEI) MAX (Polysciences, Inc., PA, USA) for 20 h ...
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bioRxiv - Microbiology 2024Quote: ... reference beads (1 μm Fluoresbrite® BB Carboxylate Microspheres; Ref. 17458, Lot. 659681; Polysciences, Inc., Warrington, PA, USA) were added as an internal sample standard ...
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bioRxiv - Microbiology 2024Quote: ... using polyethylenimine (PEI, Polysciences, Inc. ...
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bioRxiv - Cell Biology 2024Quote: ... by using polyethylenimine (PEI, Polysciences, 23966) in a 1:3 ratio (μg DNA/μg PEI ...
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bioRxiv - Physiology 2024Quote: Sirius red staining was performed on the kidney sections using a modified protocol from Polysciences. In brief ...
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bioRxiv - Cell Biology 2024Quote: ... using 90 µL 1 mg/mL Polyethylenimine Hydrochloride (Polysciences, 24765). Growth media was harvested 24-and 48-hours post-transfection ...
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bioRxiv - Microbiology 2024Quote: ... HEK293F cells were transfected using 40 kDa polyethylenamine (PEI-MAX) (Polysciences) at 1 mg ml−1 ...
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bioRxiv - Immunology 2024Quote: ... A DNA:polyethylenimine (Polysciences) ratio of 1:3 was used for transfection ...
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bioRxiv - Immunology 2024Quote: ... 3 μl of PEI-Max transfection reagent (Polysciences) (1 mg/ml ...
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bioRxiv - Immunology 2024Quote: HEK-293T cells were co-transfected with Env-expressing psvIII plasmid and a HIV Env deficient backbone plasmid (pSG3△Env) at a mass ratio of 1:2 using Polyethylenimine (PEI) max (Polysciences, Bergstrasse, Germany) at a DNA:PEI mass ration of 1:3 and incubated for 48 hours at 37°C 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... HIV-1 Env and furin protease-encoding plasmids were mixed in a 3:1 Env to furin ratio (w/w) and incubated with PEImax (Polysciences Europe GmBH, Eppelheim, Germany) in a 3:1 (w/w ...
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bioRxiv - Immunology 2024Quote: ... CA) and used an anti-rat nanoparticle for purification (Polysciences, Warrington PA).
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Hepatic iNKT cells facilitate colorectal cancer metastasis by inducing a fibrotic niche in the liverbioRxiv - Immunology 2024Quote: ... A DNA:polyethylenimine (Polysciences) µg ratio of 1:3 was used for transfection ...
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bioRxiv - Immunology 2024Quote: ... paraffin-embedded livers were sectioned at 5 µm thickness and stained with hematoxylin (Polysciences) and eosin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... 1000 µg of plasmid DNA was transfected with PEI-Max (1 mg/mL, Polysciences), at a 1:3 ratio ...
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bioRxiv - Immunology 2024Quote: ... the HLA α and β cDNA sequences were synthesized and cloned into retroviral vectors which were transfected into Phoenix packaging cells using PEI MAX (Polysciences). Supernatant containing retroviruses was used to infect mouse T cell hybridoma cells ...
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bioRxiv - Immunology 2024Quote: ... HEK293F cells were grown to a density of 2×106 cells per milliliter and transfected with plasmids encoding BTN3A1 and BTN3A2 at 1:1 ratio or BTN2A1 alone using 40 kDa linear polyethylenimine (PEI; Polysciences). For one liter of cell culture ...
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bioRxiv - Microbiology 2024Quote: ... and 2 μg of lenti-Cas9-sgRNAs using polyethylenimine (Polysciences). Culture supernatants containing lentiviruses were harvested at 48 hours post-transfection ...
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bioRxiv - Developmental Biology 2024Quote: ... guts were immediately fixed in a solution of 4% paraformaldehyde (Polysciences Inc) for 1 hour ...
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bioRxiv - Genomics 2024Quote: Lentivirus was produced by polyethylenimine linear MW 25000 (Polysciences 23966) transfection of HEK293FT cells with the transfer plasmid containing a Cas9-VPR effector ...
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bioRxiv - Cell Biology 2024Quote: ... or polyethylenimine (Polysciences, Cat # 24765). S2 cells were transfected with UAS-Inseparable-HA and Act GAL4 plasmids at 60-70% confluency ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with constructs expressing HA::IER3IP1Ref and mutant variants using Lipofectamine 3000 or polyethyleneimine (Polysciences, 24765) and cultured for 48 hours ...
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bioRxiv - Microbiology 2024Quote: ... 6.5 µg of psPAX2 and 9 µg of either GeCKO v2 human sublibrary A or B (Table S3) using PEI MAX transfection reagent (Polysciences). After 48 hours ...
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bioRxiv - Microbiology 2024Quote: ... were performed at 50% cell confluency using PEI MAX transfection reagent (Polysciences). After 24 hours ...
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bioRxiv - Microbiology 2024Quote: ... the plasmid was transfected into Expi293 cells using PEI (Polysciences). After 16 hours at 37 °C ...
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bioRxiv - Microbiology 2024Quote: Whole eyes were fixed in Davidson Fixative (Polysciences) for 24h before transitioning to 70% ethanol ...
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bioRxiv - Immunology 2024Quote: ... Four milliliters of PEI MAX® (Transfection Grade Linear Polyethylenimine Hydrochloride, MW 40,000, Polysciences, 24765-1) at 1 mg/mL was added ...
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bioRxiv - Genomics 2024Quote: ... PEI (Polysciences, catalog #24765) was used according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cells in each well were transfected with a total of 1 μg DNA (Env-expression plasmid and pCMV-rev expression helper plasmid in 4:1 ratio) mixed with 3 μg 25 kDa linear PEI (Polysciences Inc., Warrington, USA) in 200 μl 150 mM NaCl ...
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bioRxiv - Immunology 2024Quote: ... with 0.167 mg heavy chain and 0.083 mg light chain DNA using polyethylenimine 25K (Polysciences, Inc.). Six days after transfection ...
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bioRxiv - Immunology 2024Quote: ... were transfected using polyethyleneimine PEI MAX® reagent (Polysciences #24765) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cells were co-transfected using 90 µg PEI-Max (1 mg/mL, Polysciences) with 15 µg HIV- luciferase plasmid ...
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bioRxiv - Microbiology 2024Quote: ... Beads were resuspended in 1 ml of 0.1 M borate buffer (Polysciences) and 100 µg Immunoglobulin G (IgG ...
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bioRxiv - Immunology 2024Quote: ... cells were co-transfected using PEI (Polysciences, 23966) with pMD2.G ...