Labshake search
Citations for Polysciences :
201 - 250 of 2827 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... embryos were mounted in Aqua-Poly/Mount (Polysciences, Inc.) and imaged with Leica SP8 confocal microscope using 63x ...
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bioRxiv - Biophysics 2024Quote: ... 1-μm-diameter polystyrene beads (0.27%, no. 0731; Polysciences) were flowed to replace the suspension and attached to the sheared filament stubs by allowing it to stand for 4 min ...
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bioRxiv - Molecular Biology 2024Quote: One 15 cm plate of HEK293T cells was transfected with the indicated plasmids using Opti-MEMTM (31985062, ThermoFischer) and Polyethylenimine (23966-1, Polysciences), followed by harvest after 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells were transfected using polyethylenimine (PEI; Polysciences) (113 ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were mounted with Aquapolymount (18606, Polysciences), cover slipped ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... and 30 µg pAdDF6 using PEI MAX (Polysciences). At 72 hours after transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing 4 times with PBS the coverslips were mounted using Aqua-Poly/mount (Polysciences, Warrington, PA, USA). Pictures were made using the Zeiss Axio Imager 2 fluorescent microscope at a 40x zoom ...
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bioRxiv - Molecular Biology 2023Quote: Cells were fixed with 4% paraformaldehyde (PFA) (Polysciences, Warrington, United States). Unspecific binding sites were blocked by incubation with blocking buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Sections were stained with Masson’s trichrome (Polysciences, Inc., Warrington, PA, USA) to assess collagen abundance ...
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bioRxiv - Neuroscience 2023Quote: ... Aqua-Poly/Mount (#18606-20, Polysciences Inc., USA) was added and they were covered with a cover glass ...
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bioRxiv - Neuroscience 2023Quote: ... and the coverslips were mounted on glass slides using Aqua-Poly/Mount (Polysciences, 18606), and imaged on a Leica Stellaris 5 confocal microscope.
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bioRxiv - Neuroscience 2023Quote: ... cells were washed three times with DPBS before embedding with Aqua-Poly/Mount (#18606-20, Polysciences Inc., USA) and a cover glass ...
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bioRxiv - Molecular Biology 2023Quote: ... and 7.5 μg psPAX2 plasmid using 80 µL of 1mg/mL polyethylenimine (Polysciences, 25000) in 500 μL of Opti-MEM (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... 80uL of PEIMAX (Polysciences #49553-93-7) were then added ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1 were obtained by transfection of HEK293T cells with HIV-1 proviral DNA along with a VSV-G expression vector (pMD.G) and polyethylenimine (PEI) (Polysciences). Twenty-four hours after transfection ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... pMD2-VSV-G and pLKO-shRNA or pWPI expressing NS4B using 25 kDa linear polyethylenimine (Polysciences Inc.) exactly as before (Anton et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were mounted in Aquapolymount mounting medium (Polysciences Inc). For cryosections immunolabeling ...
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bioRxiv - Neuroscience 2023Quote: ... Expression plasmids were transfected using polyethylenimine (PEI) (23966-1, Polysciences). 10 ug and 200 ng of total plasmid was used for 100 mm cell culture dishes and 8-well plates respectively with a Plasmid:PEI ratio of 1:4 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: All transfections were performed by incubating DNA with the transfection reagent polyethylenimine (PEI, 25K linear, Polysciences 23966) in a 1:3 ratio in serum free DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... with linear polyethylenimine (PEI) 25 kDa (Polysciences) as transfection reagent and using 4.5 µg DNA and 9 µg PEI per mL of transfection volume ...
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bioRxiv - Molecular Biology 2023Quote: ... and linear polyethylenimine (PEI, 25K, #23966-100, Polysciences, Warrington, PA, USA) were purchased from Life-Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... transfection reagent (Polysciences, no. 23966). After 48 hours ...
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bioRxiv - Physiology 2023Quote: ... Sections were picked up on 1 x 2-mm formvar-coated copper slot grids (Polysciences) and further stained with uranyl acetate followed by lead citrate ...
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bioRxiv - Systems Biology 2023Quote: ... we added 80 µL of polyethylenimine (Polysciences #23966) to 500 µL of Opti-MEM (Fisher #31985062) ...
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bioRxiv - Systems Biology 2023Quote: ... we added 9.09 uL of polyethylenimine (Polysciences #23966) and incubated for 15 min at room temperature before adding the final mixture for each plasmid to a HEK293-containing well of the 6-well plates ...
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bioRxiv - Microbiology 2023Quote: ... and Mayer’s hematoxylin (Polysciences) was used as a counterstain ...
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bioRxiv - Neuroscience 2023Quote: ... Stained sections were mounted on glass slides with Aqua-Poly/Mount (Polysciences #18606).
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bioRxiv - Neuroscience 2023Quote: 10 cm dish transfection: 9µg of plasmids and 25µL 1µg/µL Polyethylenimine (Polysciences, Warrington, PA) were mixed in 1mL Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... At least 1×106 cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer pH 7.2 for 1 h at room temperature and subsequently incubated to 4 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Samples were washed in sodium cacodylate buffer at room temperature and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 h ...
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bioRxiv - Immunology 2023Quote: ... single cell suspension was returned to a cell incubator (37°C & 5% CO2) for 1 hour in cRPMI and fixed with 4% ultra-pure formaldehyde (#18814-20, Polysciences, Hirschberg an der Bergstraβe, Germany) for 15 minutes at RT ...
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bioRxiv - Immunology 2023Quote: ... and mounted with AquaMount (Polysciences, Inc.). Samples were visualized using a Zeiss AX10 confocal microscope ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were incubated with 4% PFA and 0.2% glutaraldehyde (Polysciences, Cat # BLI1909-10) in PBS (Corning ...
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bioRxiv - Neuroscience 2023Quote: ... Cryosections were mounted for microscopy on glass slides using Aqua-Poly/Mount (Polysciences, 18606), and imaged on a Leica TCS SP8 confocal microscope ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 2.93 µL of 1 mg/mL PEI MAX (Polysciences #24765-100) were dissolved in 300 µL of 1x PBS and applied to the cell culture ...
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bioRxiv - Neuroscience 2023Quote: ... Cultures were transfected using PEI “MAX” reagent (Polysciences, Cat 24765) with PHP.eB capsid plasmids ...
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bioRxiv - Neuroscience 2023Quote: ... Cultures were transfected using PEI “MAX” reagent (Polysciences, Cat 24765) with plasmids coding for the capsids ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected using Lipofectamine 3000 or Polyethylenimine (PEI) “MAX” reagent (Polysciences, Cat 24765), according to manufacturer’s protocols.
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bioRxiv - Neuroscience 2023Quote: ... and virus was generated by transfecting HEK293T cells with PEI Max (Polysciences, 24765); after 48 hrs the media was collected and ultracentrifuged at 17,000 rpm at 6°C for 1 hr.
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bioRxiv - Microbiology 2023Quote: ... and 2 μg plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... or pLV HA-UL38-IRES-Hygro plasmid using linear polyethyleneimine (PEI) (Polysciences, Cat.# 23966). Briefly ...
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bioRxiv - Genomics 2023Quote: ... Expi293F cells at a density of 3×106 cells/mL were transfected with 1mg/L of culture of plasmid complexed with PEI Max 40K (cat. 24765-100, Polysciences) in a 1:2 plasmid:PEI w/w ratio in 40mL Opti-MEM (cat ...
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bioRxiv - Microbiology 2023Quote: ... and gRNA containing plasmid lentiCRISPRv2 were transfected into 293T cells using polyethylenimine (Polysciences). Supernatants were collected at 48 and 72 hours post transfection ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... and then washed twice with PBS and mounted on glass slides using Aqua-Poly Mount (Polysciences).
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bioRxiv - Cell Biology 2023Quote: ... SV589 cells grown on glass coverslips were transfected with pEGFP-GM130 FL using PEI Max (Polysciences) and incubated overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Ultrathin sections (60-80 nm) were stained with uranyl acetate (Polysciences) and lead citrate (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: HEK293T cells were cultured in 12-well plates and transfected by polyethyleneimine (PEI, Polysciences) with following constructs ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell transfection was performed using polyethylenimine (PEI) (23866-1, Polysciences, Inc) according to manufacturer instructions.
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bioRxiv - Microbiology 2023Quote: HEK293T cells (2×106 cells on a 10 cm dish) were transfected with 12 μg of plasmid DNA and 36 μg of polyethylenimine hydrochloride/PEI MAX (Polysciences, Inc., PA, USA). The cells were then cultured for 20 h ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid transfections were performed with polyethylenimine “MAX” (Polysciences, Cat#24765) when HEK293A cells were grown to 60–90% confluency ...