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Citations for Polysciences :
201 - 250 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Transfections were performed with polyethyleneimine (PEI) (Polysciences, 23966-1) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... Polyethylenimine (PEI) MAXTM was used as the transfection agent (Polysciences #24765-1). Cells were washed once with PBS and a mixture of 1:1:2 (w/w ...
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bioRxiv - Biophysics 2024Quote: ... and PEI (Polysciences, Inc., Warrington, PA). After incubation for 72 h at 37 °C with 5% CO2 and 125 rpm oscillation ...
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bioRxiv - Biophysics 2024Quote: ... transfections were conducted by using PEI MAX (Polysciences). When using 60 mm dishes (Greiner ...
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bioRxiv - Biophysics 2024Quote: ... then incubated with polystyrene beads (Polysciences, USA) of 1 or 3 µm diameter in ethanol (VWR ...
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bioRxiv - Cancer Biology 2024Quote: ... rinsed 2 x 2 min with PBS and mounted with Aqua Polymount (Polysciences, 18606).
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bioRxiv - Cancer Biology 2024Quote: ... Stained cells were then mounted with Aqua-Poly/Mount (Polysciences) and imaged at 20x with a Leica AF6000 microscope and appropriate filters ...
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bioRxiv - Cancer Biology 2024Quote: HEK293T cells were transiently transfected using polyethylenimine (Polysciences); HCT-116 cells were transiently transfected with Lipofectamine 3000 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... were co-lipofected into HEK293T cells using PEI MAX (Polysciences, 24765). Supernatants containing viral particles were harvested at 2 days post-transfection and filtered through a 0.45 µm PVDF filter (Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... Multiple Stain Solution (Polysciences, Inc.) was used to stain the retina sections on slides ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with DNA (∼6ug) using polyethylenimine (PEI, Polysciences, USA) as we reported 27 ...
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bioRxiv - Cancer Biology 2024Quote: Transfections in HEK293T cells were performed using polyethylenimine (PEI; Polysciences Inc #23966-2) following standard protocols ...
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bioRxiv - Neuroscience 2024Quote: ... After mounting with Aqua-poly/mount (Polysciences 18606, Warrington, PA, USA) images were obtained with an FV1000 confocal microscope (Olympus ...
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bioRxiv - Neuroscience 2024Quote: Under isoflurane anaesthesia (1.5-2%) a small incision was made at the Achilles tendon and the tracer Fast Blue (FB, Polysciences, 1.5% in 3 µL PBS) was injected into the soleus muscle and Alexa Fluor 488-conjugated Cholera Toxin subunit B (CTB 488 ...
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bioRxiv - Neuroscience 2024Quote: ... slides were rewashed and mounted with Aqua Poly/Mount (Polysciences Inc., Ref 18606). Sections were visualized by immunofluorescence microscopy (Biorevo BZ-9000-E ...
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bioRxiv - Neuroscience 2024Quote: ... and cultures were mounted with aqueous PolyMount™ (#18606, Polysciences) onto microscope slides ...
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bioRxiv - Biophysics 2024Quote: ... 1% uranyl acetate (Polysciences, Inc) was used for staining ...
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bioRxiv - Biophysics 2024Quote: ... Transient transfection was done with polyethyleneimine (Polysciences #23966) using an ASIC:eGFP ratio of 10:1 and PEI:DNA ratio-3:1 according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... 400 μg of plasmid was pre-mixed with 1.2 mL of 1mg/mL PEI MAX (Polysciences) in 15 mL of FreeStyle 293 expression medium ...
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bioRxiv - Bioengineering 2024Quote: ... Plain polystyrene nanoparticles (∼ 80 nm) were provided by Polysciences. (www.polysciences.com).
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bioRxiv - Biophysics 2024Quote: ... Subsequent freeze-substitution and embedding of samples in Lowicryl HM20 resin (Polysciences, Inc., USA) was performed in an EM AFS2 freeze-substitution device (Leica Microsystems ...
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bioRxiv - Biochemistry 2024Quote: ... polyethyleneimine (PEI; branched, MW 2000; Polysciences) was added to a final concentration of 1% w/v to precipitate nucleic acids ...
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bioRxiv - Bioengineering 2024Quote: ... and Masson’s trichrome staining (Polysciences Inc., Warrington, PA, USA) in order to observe cell component residues and collagen distribution ...
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bioRxiv - Bioengineering 2024Quote: ... PEI MAX® - transfection grade linear polyethylenimine hydrochloride (MW 40,000) (Cat # 24765-1, Polysciences) was used for transfections ...
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bioRxiv - Bioengineering 2024Quote: ... A Fluoresbrite YG microsphere (Polysciences Inc.) was imaged as a daily standard for fluorescence lifetime ...
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bioRxiv - Biophysics 2024Quote: ... the cells (50% confluency) were transfected with 0.5 µg·well-1 plasmid DNA using polyethyleneimine (PEI Max, Polysciences) and incubated for 16 h at 37 °C in 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: Cells growing on coverslips were fixed in 4% methanol-free formaldehyde (PolySciences, 18814) in PBS and permeabilized with cold methanol ...
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bioRxiv - Cancer Biology 2024Quote: ... The transfection was performed accordingly: 2 μl polyethylenimine (PEI) 25K (1 mg/ml; Polysciences) was mixed with each μg of plasmid in Opti-MEM medium (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: 5 µm fluorescent beads (Polysciences) in PBS were used ...
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bioRxiv - Cell Biology 2024Quote: ... and SKBR3 cells were transiently transfected with a plasmid encoding CD63-Halo7 using polyethylenimine (PEI) MAX (Polysciences) to isolate tumor-derived sEVs containing CD63.
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bioRxiv - Cell Biology 2024Quote: ... and 800 ng of the desired pLenti CMV Puro DEST or pLenti-TRE-rtta3G-BLAST construct using 7 μl of 1mg/ml polyethylenimine (PEI) (Polysciences). Approximately 48 hours post transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... Tissues were embedded into disposable plastic cryomolds (Polysciences, 18646A) containing OCT (Tissue Tek 4583) ...
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bioRxiv - Cancer Biology 2024Quote: Poly(methyl methacrylate-co-methacrylic acid) (PMMA-MA; 10% methacrylic acid, MW ∼100,000 Da, 17913-500) was from Polysciences (USA), organic solvents were obtained from Carl Roth (Austria) ...
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bioRxiv - Neuroscience 2024Quote: ... were co-transfected into HEK293T cells (RCB2202; Riken BRC) using polyethylenimine (23966; Polysciences). Virus particles were purified from the cell lysate and supernatant using ultracentrifugation with OptiPrep (Serumwerk Bernburg AG ...
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bioRxiv - Neuroscience 2024Quote: ... A similar composition of microglia were plated in monoculture for phagocytosis of synaptosomes and fluorescent beads (Polysciences; Cat. No. 15702-10). Synaptosomes were prepared using iNeurons expressing the synaptophysin-Gamillus construct and SynPer (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: After washing 4 times with PBS the coverslips were mounted using Aqua-Poly/mount (Polysciences, Warrington, PA, USA). Pictures were made using the Zeiss Axio Imager 2 fluorescent microscope at a 40x zoom ...
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bioRxiv - Biophysics 2024Quote: ... HEK293 cells were reverse transfected using linear polyethyleneimine (25 kDa, Polysciences 23966) and LuTHy constructs ...
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bioRxiv - Biochemistry 2024Quote: ... 1.2 mL of 1 mg/mL PEI 25K™ (Polysciences, #23966-1) diluted in 18.8 mL Opti-MEM® ...
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bioRxiv - Cell Biology 2024Quote: ... Cationic polyethyleneimine (PEI, Polysciences GmbH) solution was used to improve transfection ...
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bioRxiv - Biochemistry 2024Quote: ... isolated lysosomes (intact or disrupted) were fixed in 2.5% glutaraldehyde (Polysciences Inc ...
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bioRxiv - Neuroscience 2024Quote: ... Food was prepared by resuspending 1 g of sera micron (Sera 00720) in 45 mL of 1/3 MR with 30 µL of 6 µm fluorescent bead solution (Polysciences 18862-1) added ...
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bioRxiv - Microbiology 2024Quote: ... HEK-293T cells and HEK-293F cells were cultured and transfected using either Lipofectamine 3000 (Thermo) or polyethylenimine (Polysciences) as previously described (30 ...
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bioRxiv - Molecular Biology 2024Quote: ... the day before transfection with polyethyleneimine (PEI-max, #24765-1 Polysciences Inc).
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bioRxiv - Microbiology 2024Quote: ... After 1-hour incubation at 37°C the treated and the untreated live HEK293 cells expressing HBc and the untransfected HEK293 cells were washed and fixed with 0.1 M sodium phosphate buffer pH 7.4 containing 4% paraformaldehyde (EM grade, Polysciences) and 1% sucrose for 10-20 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Linear poly(ethyleneimine) (PEI) (PolySciences, Warrington, PA) with a molecular weight of 25 kDa was dissolved in dH2O to 1 mM ...
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bioRxiv - Neuroscience 2024Quote: ... The transfection reagent used was polyethylenimine (Polysciences)(Chai et al. ...
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bioRxiv - Genetics 2024Quote: ... The enhancer luciferase reporter constructs with the control luciferase construct Renilla were co-transfected into cells using PEI (Polysciences). The luciferase signal was first normalized to that of the Renilla luciferase signal ...
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bioRxiv - Microbiology 2024Quote: ... vesicular stomatitis virus G (VSV-G)-pseudotyped HIV-1 viruses were produced by co-transfection of HEK293T cells with an HIV-1 proviral construct and a VSV-G-encoding vector using the PEI reagent (Polysciences). Two days post-transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... We used 0.18 m Fluoresbrite® carboxylate microspheres from Polysciences, catalog number 09834-10 ...
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bioRxiv - Microbiology 2024Quote: ... The RIG-I expression plasmid (pCAGGS-RIG-I) was transfected into wild-type or RIG-I KO 293T cells employing Polyethylenimine “Max” (PEI MAX; PolySciences, Warrington, PA, USA). The transfection of poly(I:C ...