Labshake search
Citations for Polysciences :
451 - 500 of 2827 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2023Quote: ... and pCMV-VSV-G-RSV-Rev (RIKEN BioResource Research Center plasmid RDB04393, provided by Dr. Miyoshi, Keio University) plasmids 45 using a polyethlenimine “Max” (Polysciences Inc.). Virus-containing media were collected 48 h after transfection ...
-
bioRxiv - Systems Biology 2023Quote: ... cells were transfected with 1 µg of the pTwist_CMV plasmid using 3 µg of PEI (Polysciences) as a transfection reagent or with with 1 µg of the pDEST_GFP plasmid (Control) ...
-
bioRxiv - Cell Biology 2023Quote: ... Imaging was carried out by placing adult worms in 1.5 μl of M9 mixed with 1.5 μl of 1 μm polysterene microspheres (Polysciences Inc.) on a coverslip which was mounted carefully onto a 5% agarose pad.
-
bioRxiv - Immunology 2023Quote: ... at 5 x106 cells/mL using PEI MAX (Polysciences) for DNA delivery ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 0.2ml of 2% PFA + 2% glutaraldehyde (PolySciences) per well and subsequently stained with 0.2ml of crystal violet solution (8% crystal violet + 20% ethanol in ddH2O) ...
-
bioRxiv - Biochemistry 2023Quote: ... Polyethyleneimine (PEI) was from Polysciences Inc ...
-
bioRxiv - Developmental Biology 2023Quote: ... The cells were transfected with polyethyleneimine (PEI; Polysciences). A mixture of 12 μg of the validated retroviral vectors pSIREN_EGFP-siLuc (Addgene #122287 ...
-
bioRxiv - Biochemistry 2023Quote: ... using polyethyleneimine (Polysciences), and cultured for an additional day ...
-
bioRxiv - Biochemistry 2023Quote: ... using polyethyleneimine (Polysciences, Inc., Warrington, PA, USA), and cultured for an additional day ...
-
bioRxiv - Biochemistry 2023Quote: ... HEK293T cells were transfected with PEI MAX (Polysciences, Inc., Warrington, PA, USA). UFL1 (5’-CCAGCGGGCGCAGTTCGCCG-3’ ...
-
bioRxiv - Biophysics 2023Quote: ... Purified plasmid DNA was mixed with PEI 25K (Polysciences) in a mass ratio of 3:1 (PEI ...
-
bioRxiv - Biochemistry 2023Quote: ... Flp-In Trex cells were co-transfected with a 1:9 ratio (w/w) of GFP vector:pOG44 Flp recombinase vector using PEI Max 40k (Polysciences). To select for integrant cells ...
-
bioRxiv - Biochemistry 2023Quote: ... mixed with sequence-verified plasmid DNA and PEI MAX transfection reagent (Polysciences) at a 1:3 mass ratio of DNA:PEI ...
-
bioRxiv - Biochemistry 2023Quote: ... 5 mL expression medium were supplemented with 750 μg β2m-HLA-A*68:02Fos and 750 μg TAPBPRJun DNA (equimolar ratio) and mixed with a 4-fold molar excess of linear PEI (Polysciences) diluted in 5 mL expression medium ...
-
bioRxiv - Bioengineering 2023Quote: ... Membrane associated receptor candidates were transfected by polyethylenimine (PolySciences # 23966). Cells were seeded on Neuvitro Poly-D-lysine coated sterile German glass coverslips (Fisher Scientific #NC0343705 ...
-
bioRxiv - Cell Biology 2023Quote: ... were used for lentivirus production by co-transfecting 6 μg of Lentiviral expression plasmid with 4 μg psPAX2 packaging plasmid and 0.8 μg pMD2.G envelope plasmid using the polyethylenimine transfection reagent (Polysciences). Cells with transfection medium were exchanged with fresh medium 12 hours after transfection ...
-
bioRxiv - Developmental Biology 2023Quote: ... embryos were bathed in a 1:250 solution of PSVue®643 (PSVue; Polysciences, Inc.) diluted in egg water for 1 hour prior to imaging.
-
bioRxiv - Cell Biology 2023Quote: ... cells were transfected with lentiviral packaging plasmids (pSPAX.2 and pMD2.G) and lentivirus vector Per2::dLuc using PEI Max (Polysciences). At 48 hours post-transfection ...
-
bioRxiv - Cell Biology 2023Quote: ... Polyethyenimine (PEI) (Polysciences, 23966-1) was used as a transfection reagent ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were either transfected using PEI Max 40K (Polysciences) at a DNA:PEI ratio of 1:3 (w/w ...
-
bioRxiv - Cancer Biology 2023Quote: ... Plat-E cells were seeded into 6-well plates and transfected with pMXs-Venus-IRES-PuromycinR and pMXs-HOXB7-IRES-PuromycinR plasmid DNAs using 6 μg polyethylenimine (PEI; Polysciences, Inc., PA, USA). Then ...
-
bioRxiv - Cancer Biology 2023Quote: ... Transfection was performed using Polyethylenimine (PEI) (Polysciences, 23966) according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2023Quote: ... Negative staining was performed using 1% uranyl acetate (98% uranyl acetate; ACS Reagent, Polysciences Inc. Warrington, PA, USA) solution for 20 s ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were transiently transfected with plasmid DNA using PEI (Polysciences, 24765) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... the infiltration with Lowicryl HM20 (Polysciences) was performed using increasing resin concentration in steps of 6h each ...
-
bioRxiv - Cancer Biology 2023Quote: ... lentiCRISPR v2 plasmids [81] were transfected using polyethylenimine (Polysciences) into 293T cells along with psPAX and pMD2.G packaging plasmids (Addgene ...
-
bioRxiv - Cancer Biology 2023Quote: ... The cells were transfected with 121.6 μL of 1 mg/mL of linear Polyethylenimine (PEI; MW 25,000; Polysciences) and 30.4 μg of plasmid DNA diluted in Opti-MEM (Gibco) ...
-
bioRxiv - Cell Biology 2023Quote: ... stained with DAPI and mounted on glass slides with Aqua PolyMount (Polysciences, Inc.). Images were acquired with a confocal microscope LSM700 (Zeiss ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by 3 gentle washes with PBS and finally samples were mounted on glass slides with Aqua PolyMount (Polysciences, Inc.).
-
bioRxiv - Cell Biology 2023Quote: ... were diluted 1:5 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in Milli-Q water and spun-coat onto plasma-cleaned coverslips (#1.5H ...
-
bioRxiv - Biophysics 2023Quote: ... using polyethyleneimine (PEI) (Polysciences, Cat. no: 19850) at a ratio of 3:1 (PEI:DNA 3:1) ...
-
bioRxiv - Biophysics 2023Quote: ... 5000x diluted stock solution of polystyrene beads (Polysciences, USA) in ethanol (Carl Roth ...
-
bioRxiv - Cell Biology 2023Quote: ... Slides were rinsed in cold tap water for two minutes and then stained in Beibrich Scarlett-Acid Fuchsin Solution (Polysciences) for 15 seconds ...
-
bioRxiv - Cell Biology 2023Quote: Room temperature cryosections were fixed in Bouin’s Fixative (Polysciences) for 20 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... 1% Acetic Acid (Polysciences) was pipetted directly onto the sections for one minute ...
-
bioRxiv - Cell Biology 2023Quote: ... Slides were stained in Aniline Blue (Polysciences) for 30 seconds and rinsed in DI three times for 30 seconds ...
-
bioRxiv - Cell Biology 2023Quote: ... Phosphotungstic/Phosphomolybdic acid (Polysciences) was added directly to the tissue sections ...
-
bioRxiv - Cell Biology 2023Quote: ... Slides were rinsed in cold tap water for five minutes and then stained in Weigert’s Iron Hematoxylin working solution (Polysciences) for five seconds ...
-
bioRxiv - Cell Biology 2023Quote: ... for HeLa cells or Polyethylenimine (PEI) Max (Polysciences, Warrington, PA, USA) for 293T cells ...
-
bioRxiv - Cell Biology 2023Quote: ... 25,000 Da (1mg/ml; Polysciences, Inc.). The transfection ratio was 1 μg plasmid:3 μL PEI ...
-
bioRxiv - Cell Biology 2023Quote: ... Beads (Fluoresbrite, 2 µm diameter, Polysciences, Warrington, PA) were diluted 1:500 in phosphate buffered saline ...
-
bioRxiv - Cancer Biology 2023Quote: ... All transfections were carried out using polyethyleneimine (PEI Max 40K #24765, Polysciences, Warrington, PA) reagent at a 4:2:3 ratio of sgRNA/overexpression-construct ...
-
bioRxiv - Immunology 2023Quote: ... 1.2 μg of DNA was mixed with 2.4 μL of 1 mg/mL polyethylenimine (linear PEI, MW 25,000, Polysciences) in one tenth of the final culture volume of medium ...
-
bioRxiv - Genomics 2023Quote: ... Polyethylenimine linear MW 25000 transfection grade (PEI 25K) (cat. # 239661) from Polysciences Inc. ...
-
bioRxiv - Immunology 2023Quote: 50,000 of thymocytes from Satb1+/Venus mice were resuspended in 0.1mL RPMI-1640 medium (10% FBS) and were mounted on a poly-L-lysine-coated glass slide (Polysciences, Inc., REF: 26414) and incubated at 37 °C for 2 hours ...
-
bioRxiv - Genetics 2023Quote: ... flies were dry starved for 2h and then fed Fluoresbrite microspheres (Polysciences, 17149 (0.05μm, green) or 195075 (0.5μm ...
-
bioRxiv - Genetics 2023Quote: ... or recombined forms of eG-SCON-FRT-FP was mixed with 8 μl of polyethyleneimine (1 mg/ml; Polysciences, 23966). These solutions were then incubated at room temperature for at least 15 minutes before being added dropwise to the cells ...
-
bioRxiv - Genetics 2023Quote: ... coverslips were washed twice with PBS supplemented with 0.1 % Triton-X-100 and once with PBS and mounted with aqua-poly/mount (Polysciences Inc). Cells were imaged using a Zeiss LSM700 microscope equipped with a Plan-Apochromat 40x/1.3 Oli DIC M27 immersion lens (Carl Zeiss Microimaging Inc.) ...
-
bioRxiv - Cell Biology 2023Quote: HEK293T cells (ATCC, Manassas, USA) were transfected with pNL-EGFP/pVSV using polyethylenimine (PEI; Polysciences, Germany) (PEI/DNA 6/1 ratio ...
-
bioRxiv - Cell Biology 2023Quote: ... and pMD2.G using PEI Max 40000 (PolySciences, USA). The supernatant containing lentiviral particles was harvested and mixed with forty percent PEG 8000 (Sigma Aldrich ...