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Citations for Polysciences :
451 - 500 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and 28µg PEI Max (Polysciences) in OptiMEM ...
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bioRxiv - Physiology 2024Quote: ... were transfected with packaging plasmids psPAX2 and pMD2.G to HEK293T cells using polyethylenimine (PEI, 1 mg/ml, #23966, Polysciences). Viral supernatant was harvested at 72 hrs post-transfection ...
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bioRxiv - Physiology 2024Quote: ... Slides were counterstained with eosin (0.3% in ethanol; Polysciences cat#02740).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and they were mounted with Aqua- Poly/Mount (Polysciences, 18606-20) and cover slipped ...
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bioRxiv - Physiology 2024Quote: ... and stained with Verhoeff–van Gieson for elastic fiber visualization (Polysciences, 25089-1), Von Kossa for calcification (Polysciences ...
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bioRxiv - Physiology 2024Quote: ... Von Kossa for calcification (Polysciences, 24633-1), Masson’s for collagen fibers (Polysciences ...
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bioRxiv - Physiology 2024Quote: ... Masson’s for collagen fibers (Polysciences, 25088-1) and Picrosirius Red staining for collagen quantification according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... transfection grade (PEI 25K) was obtained from Polysciences (23966-1). The selection reagents blasticidin ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cells were the exposed to 0.5 µm latex beads (carboxylated surface, yellow green-labelled, from Polysciences excitation 488 nm emission 527/32 nm) for 3 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Transfection complexes were made using PEI (1:3 ratio of DNA:PEI) (Polysciences Inc, 23966). PEI:DNA complexes were allowed to form for 15 minutes and then 50 μL of OptiMEM (Thermo ...
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bioRxiv - Plant Biology 2024Quote: ... the sections were mounted using Poly Mount (Polysciences, Inc., Warrington, PA, USA) and observed under a light microscope.
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bioRxiv - Plant Biology 2024Quote: ... samples were infiltrated with EPON812 medium-grade resin (Polysciences). Ultrathin 70 µm sections were collected on grids coated with formvar (Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2024Quote: ... Live worms were mounted on 2% agarose dry pads with 2% (for young adults) or 5% (for L1 larvae) polystyrene beads (Polybeads, Polysciences) in M9 buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Worms were immobilized by either using 20 mM levamisole in M9 or Polybead polystyrene microspheres (Polysciences) for experiments involving GABAergic stimulation ...
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bioRxiv - Neuroscience 2024Quote: ... dried on slides and covered with Aquamount (PolySciences). Sections were scanned with a Zeiss Axio Scan Z1 Slide scanner equipped with a Zeiss objective (Fluor 5×/0.25 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were transiently transfected using PEI MAX (Polysciences) and supplemented with 6 g/L glucose (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Linear 25 kDa polyethylenimine (PEI) was purchased from Polysciences (Warrington, United States). Bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were mounted using Mowiol (Polysciences) in glycerol containing 1,4,-diazobicycli-[2.2.2]octane (DABCO ...
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bioRxiv - Neuroscience 2024Quote: ... and mounted with Mowiol (Polysciences, Inc, #17951).
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bioRxiv - Neuroscience 2024Quote: ... into one 15-cm plate of confluent HEK-293T cells using PEI Max (Polysciences Inc., catalog # 24765-1). At one day post-transfection we changed the medium to low serum (1% FBS) ...
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bioRxiv - Neuroscience 2024Quote: ... Transfection was carried out with 1.1 mg plasmid and 2.2 mg linear polyethyleneimine (PEI) MAX (Mw 40,000; Polysciences Inc.) per L culture ...
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bioRxiv - Neuroscience 2024Quote: ... retinas were washed 4 times with PBS for 5 minutes prior to whole mounting onto slides with fluorescent mounting medium (Polysciences, Cat#18606-20). Slides were allowed to dry prior to imaging ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were transfected using Polyethylenimine (PEI) (1mg/mL) (Polysciences). 3µg of plasmid DNA was diluted in OptiMEM ...
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bioRxiv - Molecular Biology 2024Quote: ... a mixture of 3 μl polyethyleneimine (PEI, Polysciences), 1 μg plasmid DNA (750 ng ABEs or A&C-BEs expression plasmid and 250 ng sgRNA expression plasmid ...
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bioRxiv - Molecular Biology 2024Quote: ... a mixture of 3 μl polyethyleneimine (PEI, Polysciences), 850 ng plasmid DNA (250 ng SpCas9 sgRNA plasmid ...
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bioRxiv - Neuroscience 2024Quote: ... and mounted in Aqua-Poly/Mount (Polysciences). MEV was identified via location and characteristic PV+ large diameter cell bodies ...
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bioRxiv - Molecular Biology 2024Quote: ... coverslips were mounted with Aqua-Poly/Mount (Polysciences) to microscope slides (Epredia™ SuperFrost™ ...
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bioRxiv - Molecular Biology 2024Quote: ... coverslips were mounted with Aqua-Poly/Mount (Polysciences) to microscope slides (Epredia™ SuperFrost™ ...
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bioRxiv - Molecular Biology 2024Quote: ... coverslips were mounted with Aqua-Poly/Mount (Polysciences) to microscope slides (Epredia™ SuperFrost™ ...
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bioRxiv - Molecular Biology 2024Quote: ... and 12µg of PEI 25K (Polysciences). psPAX2 and pMD2.G were gifts from Didier Trono (Addgene #12260 ...
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bioRxiv - Molecular Biology 2024Quote: The pcDNA3.4 plasmid containing His-tagged SARS-CoV-2 spike protein was transfected to Expi293T cells using PEI max (Polysciences). After 18–21 h post-transfection ...
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bioRxiv - Molecular Biology 2024Quote: A 1 µg/µL PEI solution was prepared by dissolving 100 mg of PEI powder in 90 mL of nuclease-free water (PEI MAX® -Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000, 24765, Polysciences, Inc.). The pH was adjusted to 7.0 using 1 M HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... and embedded in Poly/bed 812 (Polysciences, Germany). 50-100 nm thin sections were cut with an Ultracut ultramicrotome (Reichert-Jung ...
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bioRxiv - Molecular Biology 2024Quote: HEK 293TN cells were transfected using Lipofectamine 2000 or PEI MAX (Polysciences; 24765-100) following the manufacturer’s protocol for lentivirus production ...
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bioRxiv - Molecular Biology 2024Quote: ... we co-transfected each transfer vector with pMD2.G and psPAX2 into 293T/17 cells using a 0.624 mg/ml PEI MAX (Polysciences, Catalog # 24765) stock solution (pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... a AAV2 Rep & AAV9 Cap gene plasmid (University of Pennsylvania, USA) and adenovirus helper plasmid (Harvard University, USA) using polyethylenimine (PEImax, Polysciences Inc). The vector was purified in an AKTAprime plus HPLC machine (AKTA prime ...
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bioRxiv - Molecular Biology 2024Quote: HEK293T cells were reverse transfected in bulk using Polyethylenimine (PEI)(Polysciences), after which equal amount of cell suspension was seeded in 6-well culture plates ...
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bioRxiv - Molecular Biology 2024Quote: ... BioMag®Plus Concanavalin A-coated magnetic beads (#86057-3, Polysciences, USA), previously activated with 20 mM HEPES pH 7.9 ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.1 µm PolyBeads (Polysciences Inc., Warrington, Pennsylvania, USA). Images were obtained with a Kinetix22 camera (Teledyne Photometrics ...
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bioRxiv - Molecular Biology 2024Quote: ... envelope plasmids using polyethylenimine (PEI) transfection (MW 25,000, Polysciences) as previously described24 ...
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bioRxiv - Molecular Biology 2024Quote: ... four L square glass bottles each containing 200 mL of suspension culture at ∼2.5 × 106 cell/mL were transfected using 1 µg/mL DNA and 3 µg/mL polyethylene ‘Max’ 40 kDa (Polysciences, Warrington, PA, #24765). The pre-premixing of DNA and PEI-Max was incubated for 20 min at room temperature before added to the bottles ...
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bioRxiv - Neuroscience 2024Quote: ... A Hamilton syringe with a 30g needle attached was inserted through the patella tendon at a depth of 2-2.5 mm to deliver the retrograde tracer fast blue (FB; 2 μl 2% in H2O; Polysciences) into the articular space ...
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bioRxiv - Physiology 2024Quote: ... fixed in 3,7% methanol-free formaldehyde (Polysciences Inc., #18814-10) for 25 min ...
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bioRxiv - Neuroscience 2024Quote: ... To facilitate the infiltration of Lowicryl HM20 (Polysciences Inc), the temperature was gradually raised to −25°C while increasing the resin concentration in acetone ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Aqua-Poly/Mount was purchased from Polysciences (Warrington, PA). Sigma 1 Receptor mouse monoclonal antibody was obtained from Santa Cruz Biotechnology (Dallas ...
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bioRxiv - Microbiology 2024Quote: ... Cells were transfected with plasmids of interest (Table 3) using polyethylenimine (PEI, Polysciences). At 4 h post-transfection ...
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bioRxiv - Microbiology 2024Quote: ... using polyethylenimine (PEI, Polysciences). 4 h post-transfection ...
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bioRxiv - Microbiology 2024Quote: ... freshly seeded 293T cells were grown in DMEM supplemented with 10% fetal bovine serum (FBS) and 50 µg/mL gentamicin at 37°C with 5% CO2 and transfected using polyethylenimine (Polysciences) [34] ...
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bioRxiv - Microbiology 2024Quote: ... using polyethylenimine (PEI, Polysciences, Inc. ...
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bioRxiv - Microbiology 2024Quote: ... mixed with 24 µL PEI (Polysciences) in a final volume of 600 µL and incubated for 1 h at room temperature ...