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Citations for Polysciences :
351 - 400 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Platinum-E cells were transfected with MDH1-PGK-GFP (for EMPTY sample) or MDH1-PGK-GFP-miR-195 or pMYs-RFP-Foxo1 using PEI MAX® (Polysciences Inc.), and retroviral supernatants were harvested 48 hours later ...
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bioRxiv - Molecular Biology 2024Quote: ... before the ROS generating agent 2,2’-Azobis(2-amidinopropane) dihydrochloride (AAPH; final conc. 1 mM in media; 08963, Polysciences, Inc., Warrington, PA, USA) was added for over-night incubation ...
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Brain-wide mapping of oligodendrocyte organization and oligodendrogenesis across the murine lifespanbioRxiv - Neuroscience 2024Quote: ... The tissue was then mounted onto slides with Aqua Polymount (18606, Polysciences) and imaged with a Zeiss LSM 880 confocal microscope (Zeiss 40× oil objective).
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Brain-wide mapping of oligodendrocyte organization and oligodendrogenesis across the murine lifespanbioRxiv - Neuroscience 2024Quote: ... the tissue was washed 4 times with 20mM PB buffer and sections were mounted onto slides with Aqua Polymount (18606, Polysciences) and allowed to dry for 24-48 hours at 4°C prior to imaging ...
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bioRxiv - Neuroscience 2024Quote: ... Ten 15-cm culture dishes with 80% confluency were transfected using 6.8 μM polyethyleneimine (Polysciences, #24765-1), 70 µg AAV transgene plasmid (pAAV-EF1a-GCaMP6s-WPRE-pGHpA ...
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bioRxiv - Neuroscience 2024Quote: ... which was formed earlier within an embedding mold (Polysciences) onto a cryostat chuck and sectioning to form a flat stage ...
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bioRxiv - Zoology 2024Quote: ... Tissue was coverslipped with an aqueous mounting medium (AquaPoly/Mount, Polysciences Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were mounted using Aqua-Poly/Mount (Polysciences cat: 18606) and cover slipped (coverslips of #1.5 thickness) ...
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bioRxiv - Molecular Biology 2024Quote: ... We used 0.18 m Fluoresbrite® carboxylate microspheres from Polysciences, catalog number 09834-10 ...
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bioRxiv - Neuroscience 2024Quote: ... Food was prepared by resuspending 1 g of sera micron (Sera 00720) in 45 mL of 1/3 MR with 30 µL of 6 µm fluorescent bead solution (Polysciences 18862-1) added ...
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bioRxiv - Cell Biology 2024Quote: ... were co-lipofected into HEK293T cells using PEI MAX (Polysciences, 24765). Supernatants containing viral particles were harvested at 2 days post-transfection and filtered through a 0.45 µm PVDF filter (Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... Multiple Stain Solution (Polysciences, Inc.) was used to stain the retina sections on slides ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with DNA (∼6ug) using polyethylenimine (PEI, Polysciences, USA) as we reported 27 ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections were performed with polyethyleneimine (PEI) (Polysciences, 23966-1) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... HEK293FT cells were seeded on 15-cm dishes without antibiotics for close to 24 hours and co-transfected with the following plasmids using Polyethylenimine (100 μg/dish, Polysciences, #23966-1): pHGTI-Adeno1 helper (22 μg/dish) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections stained with antibodies against MBP or pMLC2/Pan-NF were placed in Liberate Antibody Binding (L.A.B. Polysciences Inc.), after the initial PBS wash ...
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bioRxiv - Neuroscience 2024Quote: ... using polystyrene beads (Polysciences, 00876-15, 0.1 μM diameter) to immobilize the animals (Kim et al. ...
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bioRxiv - Neuroscience 2024Quote: ... the left sides of the brains were placed (anterior portion facing downwards) in disposable cryomold (Polysciences Inc. #18986– 1), and frozen in O.C.T ...
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bioRxiv - Neuroscience 2024Quote: ... with PEI Max (Polysciences, Cat # 24765) in HEK293T cells ...
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bioRxiv - Neuroscience 2024Quote: ... DAPI was added to the PBS at 1:2500 for nuclear staining) and then mounted on charged glass-slides and cover-slipped in Aqua Poly/Mount (Polysciences, Inc.). Primary antibodies used ...
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bioRxiv - Biophysics 2024Quote: ... Subsequent freeze-substitution and embedding of samples in Lowicryl HM20 resin (Polysciences, Inc., USA) was performed in an EM AFS2 freeze-substitution device (Leica Microsystems ...
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bioRxiv - Biochemistry 2024Quote: ... polyethyleneimine (PEI; branched, MW 2000; Polysciences) was added to a final concentration of 1% w/v to precipitate nucleic acids ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293T cells at 70% confluency in 15-cm culture plates were transfected with pFUW-GFP-AR, along with viral packaging and envelope proteins (pRSV/REV, pMDLg/RRE, and pVSV-G) using polyethylenimine transfection reagent (Polysciences; cat. #23966). 48 h post-transfection ...
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bioRxiv - Neuroscience 2024Quote: Cells were transfected with Linear Polyethylenimine 25.000 (PEI) (Polysciences, Inc., USA) or Fugene HD reagent (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... bEnd.3 cells were fixed in 4% cold PFA (Polysciences, Inc. PA, USA), and the immunofluorescence staining was performed following the same procedures as stated above.
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bioRxiv - Neuroscience 2024Quote: ... The cell pellets were fixed in cold 4% paraformaldehyde (PFA) (Polysciences, Inc. Warrington, PA, USA) at room temperature for 20 min and washed with staining medium (PBS containing 0.1% NaN3 and 2% FCS) ...
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bioRxiv - Neuroscience 2024Quote: ... HEK cell transfections were performed using the polyethylenimine (PEI) transfection reagent (Polysciences; cat. #23966) with a 3:1 PEI- to-DNA ratio when the cells reached 70% confluency ...
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bioRxiv - Neuroscience 2024Quote: ... The dyes injected included fluorescent and other tracers: Fast Blue (FB, case AT, 3% dilution, 2 µl; Polysciences), Diamidino Yellow (DY ...
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bioRxiv - Neuroscience 2024Quote: ... the transfer and pDGM6 packaging plasmids were co-transfected in 293T cells (ATCC) using PEI reagent (Polysciences, 23966-1), as described previously 52 ...
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bioRxiv - Neuroscience 2024Quote: ... A minimum of 4 weeks after viral injections fluorophore-conjugated retrograde tracers (Dextran 10,000MW, AlexaFluor 488 and 568, Invitrogen; FastBlue, Polysciences) were injected bilaterally into Area X ...
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bioRxiv - Neuroscience 2024Quote: ... 5 days after having injected HVC with the retrograde tracer Fast Blue (150nl/HVC, Polysciences) the brain was perfused with 4% paraformaldehyde (PFA) ...
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A conserved RNA switch for acetylcholine receptor clustering at neuromuscular junctions in chordatesbioRxiv - Developmental Biology 2024Quote: ... and empty vector was used to transfect each of 6 well plate(s) and 7.5 µL of linear polyethylenimine (PEI; Polysciences), MW 25,000 (1mg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... Dehydration using a graded ethanol series followed by propylene oxide incubation and resin infiltration with Polybed812 epoxy resin (Polysciences). Blocks were polymerized for 48 hours at 60°C.
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bioRxiv - Neuroscience 2024Quote: ... Cells were mounted in Aqua-Poly/Mount (Polysciences, Warrington, PA, USA). Photomicrographs for further analysis were taken with an inverted fluorescence microscope (Leica DMi 8 ...
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bioRxiv - Neuroscience 2024Quote: ... and cultures were mounted with aqueous PolyMount™ (#18606, Polysciences) onto microscope slides ...
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bioRxiv - Neuroscience 2024Quote: ... After mounting with Aqua-poly/mount (Polysciences 18606, Warrington, PA, USA) images were obtained with an FV1000 confocal microscope (Olympus ...
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bioRxiv - Neuroscience 2024Quote: Under isoflurane anaesthesia (1.5-2%) a small incision was made at the Achilles tendon and the tracer Fast Blue (FB, Polysciences, 1.5% in 3 µL PBS) was injected into the soleus muscle and Alexa Fluor 488-conjugated Cholera Toxin subunit B (CTB 488 ...
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bioRxiv - Neuroscience 2024Quote: ... which was then supplemented with 150 µl of 1 mg/mL Polyethylenimine (PEI; Polysciences Cat#23966), incubated for 10 minutes and then added to a single 15 cm plate of fully confluent cells ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293T and HeLa cells were transfected with plasmid DNA using polyethylenimine (PEI, Polysciences) for 48 hours according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... slides were rewashed and mounted with Aqua Poly/Mount (Polysciences Inc., Ref 18606). Sections were visualized by immunofluorescence microscopy (Biorevo BZ-9000-E ...
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bioRxiv - Neuroscience 2024Quote: ... and a rep/capsid plasmid using Polyethylenimine “Max” (24765-1; Polysciences, Inc., Warrington, PA, USA). Viral particles were harvested from the culture medium 6 days after transfection and were concentrated by precipitation with 8% polyethylene glycol 8000 (Merck ...
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bioRxiv - Neuroscience 2024Quote: ... A similar composition of microglia were plated in monoculture for phagocytosis of synaptosomes and fluorescent beads (Polysciences; Cat. No. 15702-10). Synaptosomes were prepared using iNeurons expressing the synaptophysin-Gamillus construct and SynPer (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: After washing 4 times with PBS the coverslips were mounted using Aqua-Poly/mount (Polysciences, Warrington, PA, USA). Pictures were made using the Zeiss Axio Imager 2 fluorescent microscope at a 40x zoom ...
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bioRxiv - Cancer Biology 2024Quote: Poly(methyl methacrylate-co-methacrylic acid) (PMMA-MA; 10% methacrylic acid, MW ∼100,000 Da, 17913-500) was from Polysciences (USA), organic solvents were obtained from Carl Roth (Austria) ...
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bioRxiv - Bioengineering 2024Quote: ... A Fluoresbrite YG microsphere (Polysciences Inc.) was imaged as a daily standard for fluorescence lifetime ...
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bioRxiv - Bioengineering 2024Quote: ... and PEO (200 kDa, Polysciences) was dissolved in 90% ethanol to yield a 10% w/v solution ...
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bioRxiv - Cancer Biology 2024Quote: ... The transfection was performed accordingly: 2 μl polyethylenimine (PEI) 25K (1 mg/ml; Polysciences) was mixed with each μg of plasmid in Opti-MEM medium (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... the cells (50% confluency) were transfected with 0.5 µg·well-1 plasmid DNA using polyethyleneimine (PEI Max, Polysciences) and incubated for 16 h at 37 °C in 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: Cells growing on coverslips were fixed in 4% methanol-free formaldehyde (PolySciences, 18814) in PBS and permeabilized with cold methanol ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmid vectors were introduced using Polyethyleneimine Max (#24765-100, Polysciences, Warrington, PA, USA) or Lipofectamine 3000 (#L3000008 ...