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Citations for Polysciences :
101 - 150 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells were transfected using polyethylenimine (PEI; Polysciences) (113 ...
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bioRxiv - Cell Biology 2024Quote: ... and finally mounted with Aquapolymount (Polysciences, 18606). Confocal images were acquired using a Nikon A1R confocal microscope with Nikon NIS-Elements software and analyzed with NIS-Elements ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were mounted in Aqua-Poly/Mount (Polysciences, Inc.) and imaged with Leica SP8 confocal microscope using 63x ...
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bioRxiv - Molecular Biology 2024Quote: One 15 cm plate of HEK293T cells was transfected with the indicated plasmids using Opti-MEMTM (31985062, ThermoFischer) and Polyethylenimine (23966-1, Polysciences), followed by harvest after 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... sections were mounted using Aqua-Poly/Mount (Polysciences) on glass slides and imaged ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were fixed in 4% methanol free PFA (Polysciences Cat# 040181) for 3 hrs at room temperature (RT ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then mounted in Mowiol mounting medium (Polysciences) in glycerol containing 2.5% 1,4-diazabicyclo-(2,2,2)-octane (DABCO ...
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bioRxiv - Cell Biology 2024Quote: ... Spreads were then overlayed by Mowiol mounting medium (Polysciences) in glycerol containing 2.5% 1,4-diazabicyclo-(2,2,2)-octane (DABCO ...
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bioRxiv - Biochemistry 2024Quote: ... Approximately 1.5 mg of plasmids were pre-mixed with 4.5 mg of polyethylenimines (PEIs) (Polysciences) in 50 mL of fresh medium for 30 min before application ...
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bioRxiv - Biochemistry 2024Quote: ... immobilization was carried out using Mowiol 4.88 (Polysciences) (supplemented with DAPI ...
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bioRxiv - Cell Biology 2024Quote: ... They were then infiltrated with three exchanges of Polybed 812 epoxy resin (Polysciences, Inc., Warrington, PA) before being embedded in fresh 100% Polybed 812 epoxy resin and allowed to cure at 60°C until hardened ...
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bioRxiv - Developmental Biology 2024Quote: ... and the corresponding packaging plasmid and polyethyleneimine (Polysciences). After 72 hours ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were mounted using Aqua Polymount (Polysciences Inc.). Images were taken at 10× magnification using an Olympus BX51 microscope equipped with an Olympus DP71 camera (Olympus) ...
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bioRxiv - Cell Biology 2024Quote: ... Embryos were mounted in Aqua-Poly/Mount (Polysciences, Inc.) prior to imaging.
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bioRxiv - Cell Biology 2024Quote: ... and Pink Flamindo were performed using 25 µg of PEI MAX (Polysciences, Warrington, PA). Following additional incubation for 24 h ...
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bioRxiv - Cell Biology 2024Quote: Polyethyleneimine (PEI, Polysciences) and FuGENE HD (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... were diluted 1:5 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in MilliQ water and spun-coat onto plasma-cleaned coverslips (#1.5H ...
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bioRxiv - Genetics 2024Quote: ... and 24 μg of PEI (1 μg/mL) (Polysciences, Warrington, PA) was directly added to the diluted DNA ...
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bioRxiv - Developmental Biology 2024Quote: ... and fixed with 4% formaldehyde (Polysciences) for 10 min ...
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bioRxiv - Genetics 2024Quote: ... The following minor modifications were made to the standard pErk mapping protocol: paraformaldehyde (PFA) (Polysciences) was diluted to 4% with 1X Phosphate Buffered Saline without 0.25% Triton ...
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bioRxiv - Genetics 2024Quote: ... 1 million HEK 293T cells in a single well of a 6-well plate were transfected using PEI-Max MW 40,000 (PolySciences, CAS Number: 49553-93-7) mixed with 1 μg of PsPax2 (Addgene # 12260) ...
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bioRxiv - Immunology 2024Quote: ... 10uL/reaction of concanavalin A-coated beads (Cat #86057-3, Polysciences) were equilibrated with Binding Buffer (20mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... Env plasmids were co-transfected with MuLV Gag plasmid and pMV-Rev 0932 in human embryonic kidney 293T (or GnT1-) cells using polyethyleneimine (PEI Max, Polysciences, Inc.). 48h later ...
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bioRxiv - Genetics 2024Quote: ... 6-10 larvae were placed in molds (Peel-A-Way disposable embedding molds, Polysciences) and sucrose solution removed ...
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bioRxiv - Immunology 2024Quote: ... BMDMs were infected Uvitex 2B (Polysciences, Warrington, PA) stained with cryptococcal cells (1.5 × 105 cells/well) ...
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bioRxiv - Microbiology 2024Quote: ... or polyethylenimine (PEI; Polysciences). The plasmids encoding replication-defective HIV-1 with firefly luciferase or mNeonGreen in place of nef (pNLdE-luc and pNLdE-mNeon ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: All transfections were performed with linear polyethyleneimine (PEI) with a molecular weight (MW) of 25,000 (Polysciences). Cells were seeded the day before transfection in culture dishes pre-treated with collagen ...
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bioRxiv - Microbiology 2024Quote: ... Calu-3 cells and HEK-293F cells were cultured and transfected using either Lipofectamine 3000 (Thermo) or polyethylenimine (Polysciences) as previously described(26 ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C in a 5% CO2 incubator for 3 days and fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Microbiology 2024Quote: ... 293T cells were co-transfected using PEI (Transporter 5 Transfection Reagent, Polysciences) at a 2:1 ratio with the Pnl4-3_inGluc vector and the spike plasmid under investigation ...
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bioRxiv - Immunology 2024Quote: ... and 1.5 mL of PEI MAX 40000 (Polysciences) or 1 mL of 293fectin in Transfectagro (Corning) ...
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bioRxiv - Immunology 2024Quote: ... HEK293T cells were transfected with the appropriate SARS-CoV-2 spike gene expression vector in conjunction with lentiviral vectors p8.91 and pCSFLW using polyethylenimine (PEI, Polysciences, Warrington, USA). Pseudotype-containing supernatants were harvested 48 hours post-transfection ...
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bioRxiv - Immunology 2024Quote: ... 1.0 μm-sized blue fluorescent beads (Polysciences Inc., Warrington, PA) were added to the culture at a target bead load of 50 per monocyte ...
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bioRxiv - Cell Biology 2024Quote: ... 60 µl of 1mg ml-1 polyethylenimine (PEI, Polysciences) and 20 µg of the retroviral vectors pMXs-Oct4 (#13366 ...
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bioRxiv - Bioengineering 2024Quote: ... nominal spring constant 0.08 N/m) were calibrated using the thermal method and one 25-μm diameter polystyrene sphere (07313-5, Polysciences) was affixed to the end of each cantilever with a small amount of UV-cured glue (AA 349 ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293FT cells were grown at 1 x 106 cells/mL and transfected with C-terminal FLAG-tagged WT or active-site mutants cloned in pcDNA3.1 using PEI-max (Polysciences #24765-100). Cells were treated with 10 mM sodium butyrate for ∼16 h after transfection and cultured for two additional days ...
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bioRxiv - Biochemistry 2024Quote: ... in a 6:4:1 ratio using PEI-max (Polysciences #24765-100). Virus-containing medium was harvested two days post-transfection ...
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bioRxiv - Biochemistry 2024Quote: HELBKO HEK293T cells were transfected with plasmids encoding pcDNA5/FRT/TO-SFB-HELB variants using PEI (Polysciences, Inc). After 24 hours ...
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bioRxiv - Biochemistry 2024Quote: HELBKO HEK293T cells were transfected with plasmids encoding pcDNA5/FRT/TO-SFB-HELB variants or pcDNA5/FRT/TO-SFB-EV (empty vector) using PEI (Polysciences, Inc). After 24 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were seeded in 35-mm glass-bottom cell-culture dishes (Iwaki) and transiently transfected with the eLACCO2.1 and deLACCO1 using polyethyleneimine (Polysciences) prior to imaging ...
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bioRxiv - Biochemistry 2024Quote: ... and 225 µl PEI (Polysciences Europe) were added ...
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bioRxiv - Biochemistry 2024Quote: ... Transfections were performed using polyethylenimine (PEI-MAX, Polysciences, 24765).
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bioRxiv - Cancer Biology 2024Quote: ... Fast Blue (5%, Polysciences) or rAAV-hSyn-EGFP (1012vp/ml ...
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bioRxiv - Biophysics 2024Quote: ... Transfections were performed using polyethyleneimine (PEI) (Polysciences Inc., Warrington, PA, USA). The day before transfection ...
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bioRxiv - Cell Biology 2024Quote: HEK 293T cells on 15 x cm2 dishes were transfected with 3.33 mg of each construct for a final 10 μg of total DNA using 25 μL polyethylenimine (PEI; Polysciences, Warrington, PA). Cells were lysed mechanically in a hypotonic solution (20 mM Tris-HCl (pH 7.0 ...
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bioRxiv - Cell Biology 2024Quote: ... PEI MAX (Polysciences, cat# 24765) was used for transfection at a ratio of 1:4 (DNA ...
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bioRxiv - Cell Biology 2024Quote: ... with DNA to PEI MAX (Polysciences, cat# 24765) ratio of 1:6 (µg:µg ...
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bioRxiv - Bioengineering 2024Quote: ... the carbon fiber was sealed with low-viscosity epoxy (Spurr Epoxy; Polysciences Inc) and left to dry overnight ...
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bioRxiv - Bioengineering 2024Quote: ... 3 µl of polyethylenimine (Polysciences) was diluted in 50 µl Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... In a second tube 3.2 µl of polyethylenimine (Polysciences) was diluted in 50 µl Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific) ...