Labshake search
Citations for Polysciences :
551 - 600 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2024Quote: ... were transfected with polyethylineimine (Polysciences) at a 4:1:1:1 ratio of the transgene and viral packaging constructs ...
-
bioRxiv - Biophysics 2024Quote: ... Tissue sections were sequentially infiltrated with increasing concentrations (10%, 25%, 50% and 75%) of Lowicryl HM20 (Polysciences, Hirschberg an der Bergstrasse ...
-
bioRxiv - Biophysics 2024Quote: ... A total of 10 mL (1 mg/mL in PBS) of PEI Max 40 kDa (Polysciences Inc.) was added to the DNA and incubated for 20 min at room temperature ...
-
bioRxiv - Genomics 2024Quote: ... and 10 μL polyethylenimine (PEI, Polysciences Inc. 239661, 1 mg/mL). 24 hr after transfection ...
-
bioRxiv - Genomics 2024Quote: ... and Rep/Cap plasmids using polyethyleneimine transfection (PEI MAX, Polysciences) at approximately 90% confluency ...
-
bioRxiv - Bioengineering 2024Quote: ... N-(3-aminopropyl)methacrylamide hydrochloride (APMA) was obtained from Polysciences Inc (Warrington ...
-
bioRxiv - Cancer Biology 2024Quote: ... using 48 μg of Transporter 5 Transfection Reagent (Polysciences), following manufacturer’s instructions in T25 flasks ...
-
bioRxiv - Cancer Biology 2024Quote: Second generation of lentiviral transfection system was used to generate viral particle containing shRNA using PEI reagent (Polysciences) as described by Paul et al. ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The 4 plasmids were incubated for 15 minutes with 5 μL of polyethylenimine (PEI, Polysciences #23966) before transfection ...
-
bioRxiv - Plant Biology 2024Quote: ... 4 µL of 1 mg/mL of polyethylenimine HCl MAX Linear MW 40k (PEI, Polysciences) in 25 mM HEPES pH 7.5 was applied and incubated on glow discharged grids for 2 min ...
-
bioRxiv - Genomics 2024Quote: ... 10 μl of BioMag Plus Concanavalin-A-conjugated magnetic beads (ConA beads, Polysciences, Inc) in binding buffer (20 mM HEPES-KOH pH 7.9 ...
-
bioRxiv - Neuroscience 2024Quote: ... at a 1:1:1 molar ratio using PEI-polyethyleneimine “max,” (Polysciences #24765). Media was changed to serum-free DMEM at 16 hours post-transfection ...
-
bioRxiv - Cell Biology 2024Quote: ... and mounted with Aqua-Poly/Mount (Polysciences). Confocal microscopy images were acquired using a Nikon AX ...
-
bioRxiv - Neuroscience 2024Quote: ... The procedure involved mounting the worms on a glass slide with a 10% agar pad derived from NGM buffer and using a coverslip to immobilize them with 0.2 µm latex beads (Polysciences, Inc.). Ablation of PHD neuron nuclei on both sides was achieved using the MicroPoint (Andor ...
-
bioRxiv - Neuroscience 2024Quote: ... Approximately 5 ul of 0.2 um latex beads (Polysciences, Inc.) were added to aid immobilization ...
-
bioRxiv - Genomics 2024Quote: ... using polyethyleneimine linear MW 25000 (Polysciences). Virus supernatant was harvested 72 h post transfection and filtered through a 0.45 μm Steriflip-HV filter (Millipore) ...
-
bioRxiv - Molecular Biology 2024Quote: HEK293 cells were transfected using JetPEI® (Polyplus-transfection) or polyethylenimine (PEI) 25K (Polysciences) prepared at 1 mg/mL with a protocol similar to the commercial JetPEI reagent ...
-
bioRxiv - Genetics 2024Quote: ... Verhoeff Van Gieson (VVG, Polysciences, Inc., 25089-1), and Picro-Sirius Red (Abcam ...
-
bioRxiv - Genetics 2024Quote: ... A drop of Aquapolymount (Polysciences, Warrington, PA) was placed over the sample and cover with a No ...
-
bioRxiv - Microbiology 2024Quote: ... The specimens were embedded with a Poly/Bed 812 kit (Polysciences Inc., USA) and polymerized in an electron microscope oven (TD-700 ...
-
bioRxiv - Genomics 2024Quote: ... CaGHV-1 RTA was expressed as an N-terminally 3xFLAG-tagged protein in HEK293T cells by transfecting the cells with PEI (Polysciences). The CaGHV-1 ORF50 promoter fragments were PCR amplified and cloned into pGL4.15 luciferase reporter vector (Promega ...
-
bioRxiv - Biochemistry 2024Quote: ... packaging plasmids at a 5:3.75:1.25 ratio to HEK293T cells using PEI transfection reagent (Polysciences Inc., 23966). Viral supernatants were collected 48 h and 72 h post-transfection and filtered using a 0.45 μm syringe filter ...
-
bioRxiv - Cell Biology 2024Quote: ... infiltrated and embedded in situ in PolyBed 812 epoxy resin (Polysciences, Inc, Warrington, PA). The cell monolayer was sectioned en face to the substrate with a diamond knife and Leica UCT Ultramicrotome (Leica Microsystems ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The cells were subsequently co-transfected with corresponding plasmid mixtures for 6 hours with 50 μL PEI (Catalog no. 24765, Polysciences; molecular weight 40,000 ...
-
bioRxiv - Neuroscience 2024Quote: ... using PEI Max (Polysciences, Inc., #24765-1). Twenty-four hours after transfection ...
-
bioRxiv - Neuroscience 2024Quote: ... following previously published protocol.42 Triple transfection was performed with Transporter 5 Transfection Reagent (Polysciences 26008). AAVs were harvested 72 hours post-transfection and purified on iodixanol gradient ...
-
bioRxiv - Biochemistry 2024Quote: ... in 2 L flasks using polyethylenimine (PEI, Polysciences) reagent according to the manufacturer’s protocol in an incubator at 37°C and 8% CO2 under shaking (125 rpm) ...
-
bioRxiv - Bioengineering 2024Quote: ... A Fluoresbrite YG microsphere (Polysciences Inc.) was imaged as a daily standard for fluorescence lifetime ...
-
bioRxiv - Bioengineering 2024Quote: Yellow-green fluorescent beads of 0.2 µm diameter (Polysciences, Cat# 17151) were dried onto glass slides ...
-
bioRxiv - Bioengineering 2024Quote: ... isolated with ultracentrifugation were resuspended and incubated in 2.5% glutaraldehyde for 1 hour at room temperature and loaded on plasma cleaned carbon-coated copper grid (Polysciences). Fixed EV samples were incubated on the grids for 20 min at room temperature ...
-
bioRxiv - Bioengineering 2024Quote: ... AEMA (2.54 g, Polysciences) was then slowly added to the solution to achieve a theoretical methacrylation level of 30% ...
-
bioRxiv - Bioengineering 2024Quote: ... Alcian blue (Fisher, cat#92-31-9) and nuclear fast red (Polysciences, cat# 09773) sections were stained with alcian blue (pH = 0.2 ...
-
bioRxiv - Bioengineering 2024Quote: ... 293T cells were transfected using PEI MAX® solution (Polysciences, Warrington, PA) with pAAV-CBA-Cre-p41-Cap9-7mer (Round 1 of selection ...
-
bioRxiv - Bioengineering 2024Quote: ... 39.9 μL of 1 mg/mL PEI MAX (Polysciences) and 1 mL of DMEM were mixed by inversion and incubated at room temperature for 10 min ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... and cover-slipped with Aquamount (Polysciences, Inc., Warrington, PA) without additional staining.
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... and cover-slipped with Aquamount (Polysciences, Inc., Warrington, PA). For fiber photometry and optogenetics experiments ...
-
bioRxiv - Bioengineering 2024Quote: ... AEMA (0.844 and 1.688 g, Polysciences) (molar ratio of NHS:EDC:AEMA = 1:2:1 ...
-
bioRxiv - Bioengineering 2024Quote: ... 5 mL 40 wt% Poly(ethylene glycol) diacrylate (PEGDA) 20k (Polysciences), 200 µL of 5 wt% Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP ...
-
bioRxiv - Biochemistry 2024Quote: ... Transient transfection of the indicated plasmids was performed using Polyethylenimine “Max”(PEImax) (#24765, Polysciences Inc., Warrington, PA, USA) according to the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2024Quote: ... this solution includes 100 µg of plasmid mixed with 300 µl of a 1 mg/ml solution of polyethyleneimine (PEI MAX, Polysciences) in 4 mL of OptiPRO SFM media (ThermoFisher Scientific) ...
-
bioRxiv - Biochemistry 2024Quote: ... HEK293 cells were transfected with a total of 2 µg DNA per 1 x 106 cells using 6 µg polyethyleneimine (PEI, Polysciences Inc.) as a transfection reagent in 150 mM NaCl ...
-
bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected at 60-70% confluence using polyethyleneimine (PEI) as the transfection reagent from Polysciences (cat. no. #24765-1).The cell medium was replaced with conditioned culture medium 5 hours post-transfection ...
-
bioRxiv - Biophysics 2024Quote: Standard microplastic concentrations were created by an initial dilution of commercial bead samples of known concentration (2.5% w/v 3 µm PS beads, Polysciences; 10% w/v 4 µm PMMA beads ...
-
bioRxiv - Cancer Biology 2024Quote: ... Lentiviruses for FUCCI reporters and pGAMA-YAP-S127A were produced in 293T cells using standard polyethylenimine (Polysciences Inc.) transfection protocols.
-
bioRxiv - Cancer Biology 2024Quote: ... cells were triple transfected using polyethylenimine Max (PEI Max, Polysciences 24765-1) with 12 µg of pAAVhelper ...
-
bioRxiv - Biophysics 2024Quote: ... Negative staining was performed using 1% uranyl acetate (98% uranyl acetate; ACS Reagent, Polysciences Inc. Warrington, PA, USA) solution for 20 s ...
-
bioRxiv - Biophysics 2024Quote: ... HEK293T cells were transfected at 70% confluency in a 6-well plate or 12-well plate using PEI MAX® - Transfection Grade Linear Polyethyleneimine Hydrochloride (MW 40,000) (Polysciences) at a 1:3 DNA ...
-
bioRxiv - Biophysics 2024Quote: ... diluted 1:100 in nanopure water were diluted 1:10 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in nanopure water and spin coated (WS-650 ...
-
bioRxiv - Biophysics 2024Quote: ... 1.2 mL of 1 mg/mL PEI MAX (PolySciences) was premixed with 400 µg of plasmid in 15 mL of FreeStyle expression medium and incubated for 20 minutes at room temperature prior to transfection ...
-
bioRxiv - Genetics 2024Quote: ... into cells using PEI (Polysciences, #24765) or lipofectamine 3000 (Invitrogen ...