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Citations for Polysciences :
551 - 600 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... CaGHV-1 RTA was expressed as an N-terminally 3xFLAG-tagged protein in HEK293T cells by transfecting the cells with PEI (Polysciences). The CaGHV-1 ORF50 promoter fragments were PCR amplified and cloned into pGL4.15 luciferase reporter vector (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... Transient transfection of the indicated plasmids was performed using Polyethylenimine “Max”(PEImax) (#24765, Polysciences Inc., Warrington, PA, USA) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... HEK293 cells were transfected with a total of 2 µg DNA per 1 x 106 cells using 6 µg polyethyleneimine (PEI, Polysciences Inc.) as a transfection reagent in 150 mM NaCl ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cells were subsequently co-transfected with corresponding plasmid mixtures for 6 hours with 50 μL PEI (Catalog no. 24765, Polysciences; molecular weight 40,000 ...
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bioRxiv - Bioengineering 2024Quote: ... A Fluoresbrite YG microsphere (Polysciences Inc.) was imaged as a daily standard for fluorescence lifetime ...
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bioRxiv - Bioengineering 2024Quote: ... isolated with ultracentrifugation were resuspended and incubated in 2.5% glutaraldehyde for 1 hour at room temperature and loaded on plasma cleaned carbon-coated copper grid (Polysciences). Fixed EV samples were incubated on the grids for 20 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... in 2 L flasks using polyethylenimine (PEI, Polysciences) reagent according to the manufacturer’s protocol in an incubator at 37°C and 8% CO2 under shaking (125 rpm) ...
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bioRxiv - Bioengineering 2024Quote: ... 39.9 μL of 1 mg/mL PEI MAX (Polysciences) and 1 mL of DMEM were mixed by inversion and incubated at room temperature for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... this solution includes 100 µg of plasmid mixed with 300 µl of a 1 mg/ml solution of polyethyleneimine (PEI MAX, Polysciences) in 4 mL of OptiPRO SFM media (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected at 60-70% confluence using polyethyleneimine (PEI) as the transfection reagent from Polysciences (cat. no. #24765-1).The cell medium was replaced with conditioned culture medium 5 hours post-transfection ...
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bioRxiv - Bioengineering 2024Quote: ... AEMA (2.54 g, Polysciences) was then slowly added to the solution to achieve a theoretical methacrylation level of 30% ...
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bioRxiv - Bioengineering 2024Quote: ... Alcian blue (Fisher, cat#92-31-9) and nuclear fast red (Polysciences, cat# 09773) sections were stained with alcian blue (pH = 0.2 ...
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bioRxiv - Bioengineering 2024Quote: ... 293T cells were transfected using PEI MAX® solution (Polysciences, Warrington, PA) with pAAV-CBA-Cre-p41-Cap9-7mer (Round 1 of selection ...
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bioRxiv - Bioengineering 2024Quote: Yellow-green fluorescent beads of 0.2 µm diameter (Polysciences, Cat# 17151) were dried onto glass slides ...
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bioRxiv - Bioengineering 2024Quote: ... AEMA (0.844 and 1.688 g, Polysciences) (molar ratio of NHS:EDC:AEMA = 1:2:1 ...
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bioRxiv - Bioengineering 2024Quote: ... 5 mL 40 wt% Poly(ethylene glycol) diacrylate (PEGDA) 20k (Polysciences), 200 µL of 5 wt% Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP ...
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bioRxiv - Genetics 2024Quote: ... and 3xF-BRCA1-2A- mRuby3 repair template plasmid in 50 μL of PEI MAX® (Polysciences, 24765) with OptiMEM (Gibco ...
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bioRxiv - Biophysics 2024Quote: ... 100 µg of plasmid DNA was incubated with 300 µg of polyethylenimine (PEI 25,000) (Polysciences, 23966-1) for 30 min at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... were transfected with 15 μg per dish of RyR1-W882A cDNA using PEI MAX (Polysciences). Cells were collected 48 hours after transfection ...
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bioRxiv - Biophysics 2024Quote: ... HEK293T cells were transfected at 70% confluency in a 6-well plate or 12-well plate using PEI MAX® - Transfection Grade Linear Polyethyleneimine Hydrochloride (MW 40,000) (Polysciences) at a 1:3 DNA ...
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bioRxiv - Biophysics 2024Quote: ... diluted 1:100 in nanopure water were diluted 1:10 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in nanopure water and spin coated (WS-650 ...
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bioRxiv - Biophysics 2024Quote: ... 1.2 mL of 1 mg/mL PEI MAX (PolySciences) was premixed with 400 µg of plasmid in 15 mL of FreeStyle expression medium and incubated for 20 minutes at room temperature prior to transfection ...
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bioRxiv - Genetics 2024Quote: ... into cells using PEI (Polysciences, #24765) or lipofectamine 3000 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were triple transfected using polyethylenimine Max (PEI Max, Polysciences 24765-1) with 12 µg of pAAVhelper ...
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bioRxiv - Biophysics 2024Quote: ... Negative staining was performed using 1% uranyl acetate (98% uranyl acetate; ACS Reagent, Polysciences Inc. Warrington, PA, USA) solution for 20 s ...
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bioRxiv - Bioengineering 2024Quote: ... MW 25000 (Polysciences #23966) at a PEI-to-DNA ratio of 4-to-1 ...
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bioRxiv - Bioengineering 2024Quote: ... cells were then fixed in 4% formaldehyde (Polysciences, Inc., 18814) for 15 min at room temperature ...
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bioRxiv - Bioengineering 2024Quote: 20 µm Polystyrene beads (18329-5, Polysciences, USA) were embedded in NOA73 (NOA73 ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were washed again for 0.5 hours in PBS and mounted with Aquapolymount (18606, Polysciences).
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bioRxiv - Developmental Biology 2024Quote: ... Colorimetric samples were mounted with Aqua-polymount (Polysciences).
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bioRxiv - Zoology 2024Quote: ... Exsheathed larvae and adult worms were cultured in the presence/absence of 0.5 mm FITC labelled and/or 6 mm PE labelled fluorescent beads (Polysciences) for 5 days at 0.5µM (1 x107 beads/ml) ...
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bioRxiv - Developmental Biology 2024Quote: ... Aqua-Poly/Mounted samples (Polysciences, 18606-20) were imaged with a Leica SP5 confocal microscope ...
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bioRxiv - Cell Biology 2024Quote: ... and PEI (1 mg/mL, Polysciences). The supernatant was collected 48 hours after transfection and the resulting lentivirus preparation was concentrated using Lenti-XTM Concentrator (Clontech ...
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bioRxiv - Developmental Biology 2024Quote: ... all access PBS++ was removed from the tube and replaced by 200 µl mounting medium composed of 50:50 Aqua-Poly Mount (Polysciences 18606-20) and PBS++ ...
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bioRxiv - Developmental Biology 2024Quote: ... dried and mounted (Polysciences 18606-20). All immunofluorescence slides were also stained with 4 ...
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bioRxiv - Systems Biology 2024Quote: 4.26 pmol of plasmid DNA (15-20 µg) and PEI 25K™ (Polysciences, 23966) (DNA:PEI = 1:5 w/w ...
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bioRxiv - Synthetic Biology 2024Quote: ... 30 µg plasmid was complexed with 45 µg polyethyleneimine (Polysciences; 24765-1) in 4 mL Opti-MEM (Gibco ...
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bioRxiv - Systems Biology 2024Quote: ... 250.000 cells were captured with BioMagPlus Concanavalin A (Polysciences, 86057-3) and incubated with the appropriate primary antibody overnight at 4°C (anti-Histone H3 (tri methyl K4 ...
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bioRxiv - Bioengineering 2024Quote: ... 293T cells were triple transfected using PEI MAX® solution (Polysciences, Warrington, PA) with (1 ...
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bioRxiv - Synthetic Biology 2024Quote: ... or 60µl of 1mg/ml linear PEI (Polysciences, cat. 23966-1) was diluted in OptiMEM buffer to a final volume of 600µl ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviruses for FUCCI reporters and pGAMA-YAP-S127A were produced in 293T cells using standard polyethylenimine (Polysciences Inc.) transfection protocols.
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bioRxiv - Synthetic Biology 2024Quote: Transient transfection was performed using polyethylenimine (PEI) (Polysciences, 23966, prepared as a .323 g l-1 stock). For each transfected well of a 24-well plate ...
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bioRxiv - Biophysics 2024Quote: ... Dechorionated embryos were fixed in 10% formaldehyde (methanol free, 18814 Polysciences Inc.) in PBS:Heptane (1:1 ...
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bioRxiv - Biophysics 2024Quote: ... were transfected with polyethylineimine (Polysciences) at a 4:1:1:1 ratio of the transgene and viral packaging constructs ...
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bioRxiv - Biophysics 2024Quote: ... Tissue sections were sequentially infiltrated with increasing concentrations (10%, 25%, 50% and 75%) of Lowicryl HM20 (Polysciences, Hirschberg an der Bergstrasse ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were washed with PBST and mounted using Aqua-Poly/Mount (Polysciences, Inc., Warrington, PA, USA).
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were then mounted in Aqua-Poly/Mount (Polysciences, Inc). Confocal images were taken using a Leica TCS SP8 confocal microscope with 63x ...
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bioRxiv - Biophysics 2024Quote: ... A total of 10 mL (1 mg/mL in PBS) of PEI Max 40 kDa (Polysciences Inc.) was added to the DNA and incubated for 20 min at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... were transfected by PEI MAX (Polysciences) with Lifeact-GFP plasmid (pLVX LifeAct-eGFP-P2A-puro ...
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bioRxiv - Cancer Biology 2024Quote: ... Coverslips were mounted using 10% (wt/vol) Mowiol 4-88 (Polysciences). Epifluorescence images were captured using a Nikon Eclipse NiE (20×/0.75 Plan Apo Nikon objective ...