Labshake search
Citations for Polysciences :
401 - 450 of 2827 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with polyethyleneimine (PEI) (Polysciences; #23966-1) with pCMV-dR8_91 and pVSV-G packaging plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... and Sirius Red (cat# 24901250; Polysciences, Inc) staining was performed per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... embedded in Lowicryl HM20 resin (Polysciences, Warrington, PA), UV polymerized at −60°C warming slowly over 4 days to room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cover slips were then washed again and mounted onto glass slides using Aqua Polymount (Polysciences). Fluorescence microscopy ...
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bioRxiv - Bioengineering 2023Quote: ... 15-micron polystyrene beads (Polysciences Polybead microsphere 15 µm catalogue number 18328-5) ...
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bioRxiv - Biophysics 2023Quote: ... polystyrene reference beads (Polysciences Europe) of 1.5 µm in diameter were diluted 1:1500 in PBS buffer (pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... The cells in each well were transiently transfected with 1 μg DNA plasmid using 3μg linear polyethyleneimine (PEI, MW 25000, Polysciences) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Ovaries were embedded in JB-4 Plus plastic resin (Polysciences) and 5-10 μm sections were cut using a Leica RM 2155 microtome ...
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bioRxiv - Developmental Biology 2023Quote: ... stained in Eosin Y (Polysciences), washed in distilled water ...
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bioRxiv - Bioengineering 2023Quote: ... we combined 100 ng of DNA with 1 μL of PEI MAX solution (polyethyleneimine, Polysciences) in 10 μL of DMEM and incubated at room temperature for 10 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Polyethyleneimine (linear, 25 kDa) was purchased from Polysciences (Warrington, PA, USA). Lenti-X concentrator was purchased from Takara Bio (Kusatsu ...
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bioRxiv - Biochemistry 2023Quote: ... in 2 L flasks using polyethylenimine (PEI, Polysciences) reagent according to the manufacturer’s protocol in an incubator at 37°C and 8% CO2 under shaking (125 rpm) ...
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bioRxiv - Bioengineering 2023Quote: ... we added 80 µL of polyethylenimine (Polysciences #23966) to 500 µL of Opti-MEM (Fisher #31985062 ...
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bioRxiv - Bioengineering 2023Quote: ... The light and heavy chain expression plasmids of LH03 or LH05 were mixed with 25 kDa linear polyethylenimine (PEI, Polysciences) and transiently transfected in HEK293E cells ...
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bioRxiv - Genetics 2023Quote: ... We resuspended pupal abdomens in 15 ul of BioMag Plus Concanavalin-A-conjugated magnetic beads (ConA beads, Polysciences Inc.) and followed a CUT&RUN protocol for Drosophila tissues (https://dx.doi.org/10.17504/protocols.io.umfeu3n) ...
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bioRxiv - Genomics 2023Quote: ... were generated using polyethylenimine (PEI, Polysciences, #23966-2)-mediated transfection of human embryonic kidney 293T (HEK293T ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were mounted using Polymount (Polysciences,18606-100).
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bioRxiv - Microbiology 2023Quote: ... Monolayers were washed twice with DPBS and fixed in freshly prepared mixture of 4% paraformaldehyde and 0.05% glutaraldehyde (Polysciences Inc.) in 100 mM PIPES/0.5 mM MgCl2 buffer (pH 7.2 ...
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bioRxiv - Microbiology 2023Quote: ... and 1% osmium tetroxide (Polysciences Inc.) in 50 mM phosphate buffer for 30 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... FACS buffer was removed and pellets were fixed in freshly prepared 2% methanol free formaldehyde (diluted from 16% Ultra Pure EM grade formaldehyde (Polysciences, Warrington, PA, USA) for a minimum of 1 hour prior to analysis ...
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bioRxiv - Microbiology 2023Quote: ... 1 million HEK293T cells were seeded into 6-well plates and transfected using polyethylenimine (PEI) (#23966-1; Polysciences, Inc.) with 1 μg of the indicated DNA constructs at a 1:5 ratio with PEI ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected using polyethylenimine (PEI) (#23966- 1; Polysciences, Inc.) with 5 μg of the indicated DNA constructs using a 1:5 ratio with PEI ...
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bioRxiv - Neuroscience 2023Quote: ... coverslips or sections were mounted with Aqua Poly/Mount (Polysciences) and imaged.
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bioRxiv - Molecular Biology 2023Quote: ... Each dish was then transfected with 112μg polyethylenimine (cat. 23966; Polysciences, Inc. Warrington, PA, USA), 6μg ITR-containing plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... Producer cells were transfected with a solution containing the selected lentiviral vector transgene backbone, the packaging plasmids pMDLg/pRRE and pCMV.REV, pMD2.G (Plasmid Factory, Bielefeld, Germany) and polyethylenimine (PEI) (24765, Polysciences, Warrington, US). Transfection media was changes after 4 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Sections were counterstained with DAPI and cover slipped with Aqua-Poly/Mount (Polysciences). Tissue sections were imaged on an Axioscan 7 (Zeiss ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM Spermidine) and bound to 10 µl activated BioMag®Plus Concanavalin A magnetic beads (Polysciences) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... After keeping them on ice for no longer than 20 minutes they were fixated for 50 minutes in 2 % paraformaldehyde (PFA, Polysciences, diluted in PBS) while shaking at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... Aquamount (Polysciences, 18606) was used to mount coverglass slides ...
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bioRxiv - Neuroscience 2023Quote: ... with 112 μg PEI MAX (Polysciences, Inc.) as a transfection reagent ...
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bioRxiv - Neuroscience 2023Quote: ... The HEK293T cells were transfected using 1 mg/mL Polyethylenimine (PEI; 23966, Polysciences) in a ratio of 3 μL to 1 μg DNA ...
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bioRxiv - Neuroscience 2023Quote: ... Cultures were transfected using PEI “MAX” reagent (Polysciences, Cat 24765) with PHP.eB capsid plasmids ...
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bioRxiv - Immunology 2023Quote: ... Expi293 cells were used for transient transfection with the suitable S2P stabilized S-expression plasmids or other vectors by using 1 mg/mL polyethylenimine (PEI, Polysciences). The supernatant was harvested and the S trimer was purified using Ni-NTA resin (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... and finally 2 washes in dH2O prior to mounting on glass slides with Mowiol 4-88 (Polysciences Inc.). Mounting medium was allowed to harden overnight ...
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bioRxiv - Immunology 2023Quote: ... and coverslipped with Aqua/Poly/Mount (Polysciences). Immunfluorescence was analyzed using a Nikon Eclipse Ti-E inverted microscope (Nikon Instruments Europe B.V. ...
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bioRxiv - Neuroscience 2023Quote: ... mounted with Poly Aqua-Mount (Polysciences, 18606), and imaged using a confocal microscope (Zeiss LSM 880 or Zeiss LSM 700).
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bioRxiv - Microbiology 2023Quote: ... DNA was introduced into cells using polyethyleneimine MAX 40K (PEI MAX) (Polysciences) at a ratio of 3:1 PEI:total DNA or TransIT-LT1 (Mirus ...
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bioRxiv - Systems Biology 2023Quote: ... using 4.5 µg of PEI (Polysciences) as a transfection reagent ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Polyethylenimine (PEI) [linearpoly(vinyl alcohol) (MW-25000)] was from Polysciences (Warrington, PA). Molecular biology enzymes and reagents were from Promega (Southampton ...
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bioRxiv - Genomics 2023Quote: ... at 5 x106 cells/mL using PEI MAX (Polysciences) for DNA delivery96 ...
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bioRxiv - Immunology 2023Quote: ... using Polyethylenimine (PEI, Polysciences) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: The 25kDa PEI 25™ (PEI-25) and 40 kDa PEI Max® (PEI-40) polymers were acquired from Polysciences and were prepared according to manufacturer instructions to get a stock solution of 1 mg/ml ...
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bioRxiv - Immunology 2023Quote: ... using Polyethylenimine (PEI, Polysciences). After 5 days ...
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bioRxiv - Immunology 2023Quote: ... 3 μm (#19118-2; Polysciences) or 6 μm (#17145-4 ...
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bioRxiv - Immunology 2023Quote: ... Expi293 cells were transiently transfected with plasmid DNA mixed with PEI (Polysciences, Warrington, PA) and incubated for 5 days at 37 degC ...
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bioRxiv - Immunology 2023Quote: ... and 2.2 μg/ml polyethylenimine (linear 25 kDa PEI, Polysciences, Inc, Warrington, PA) to the suspension cultures ...
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bioRxiv - Microbiology 2023Quote: ... 500 µL PEI-max (1 µg/µL; Polysciences) was mixed with DNA (1 µg/mL cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Packaging cells were transfected using polyethyleneimine (Polysciences, 23966-2) by mixing with DNA in a 3:1 ratio ...
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bioRxiv - Neuroscience 2023Quote: ... and coverslipped with Aqua-Poly/Mount (Polysciences). Immunofluorescence was analyzed using a Nikon Eclipse Ti-E inverted microscope (Nikon Instruments Europe B.V. ...
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bioRxiv - Immunology 2023Quote: ... For assessment of phosphorylated proteins cells were fixed in 4% ultra-pure formaldehyde (#18814-20, Polysciences) for 10 minutes at 37°C directly after cell-culture ...