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Citations for Polysciences :
401 - 450 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... with 1 mg of total DNA and 4 mg of PEI (Polysciences, 24765) per 1 liter of cells at a density of 1.5-2 x 106 cells/ml ...
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bioRxiv - Neuroscience 2024Quote: ... were co-transfected into HEK293T cells (RCB2202; Riken BRC) using polyethylenimine (23966; Polysciences). Virus particles were purified from the cell lysate and supernatant using ultracentrifugation with OptiPrep (Serumwerk Bernburg AG ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected with various plasmids using PEI (Polysciences); HCT-116 and U-2 OS where transiently transfected with Lipofectamine 3000 (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Transient transfections were carried out using polyethylenimine (PEI) (Polysciences, Cat#23966). Cells were collected 48 hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... 20ug Packaging plasmid (pCMV-deltaR8.9 or psPAX2) and 6.25ug Envelope plasmid (pCMV-VSVg or pMD2.G) were transfect with PEI-MAX (Polysciences, 24765-100) when cells reached to 70% in 15cm dish ...
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bioRxiv - Neuroscience 2024Quote: ... The transfection reagent used was polyethylenimine (Polysciences)(Chai et al. ...
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bioRxiv - Genetics 2024Quote: ... The enhancer luciferase reporter constructs with the control luciferase construct Renilla were co-transfected into cells using PEI (Polysciences). The luciferase signal was first normalized to that of the Renilla luciferase signal ...
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bioRxiv - Pathology 2024Quote: ... Ly6Chi monocytes were labeled by intravenous injection of 1.0 µm Fluoresbrite® YG Microspheres (Polysciences, Inc) 2 days before sacrifice.
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bioRxiv - Biochemistry 2024Quote: ... 1.2 mL of 1 mg/mL PEI 25K™ (Polysciences, #23966-1) diluted in 18.8 mL Opti-MEM® ...
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bioRxiv - Biochemistry 2024Quote: ... isolated lysosomes (intact or disrupted) were fixed in 2.5% glutaraldehyde (Polysciences Inc ...
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bioRxiv - Microbiology 2024Quote: ... and 5 µg/mL Uvitex 2B (Polysciences Inc., Warrington, PA) to label cell wall chitin ...
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bioRxiv - Biophysics 2024Quote: ... HEK293 cells were reverse transfected using linear polyethyleneimine (25 kDa, Polysciences 23966) and LuTHy constructs ...
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bioRxiv - Microbiology 2024Quote: ... vesicular stomatitis virus G (VSV-G)-pseudotyped HIV-1 viruses were produced by co-transfection of HEK293T cells with an HIV-1 proviral construct and a VSV-G-encoding vector using the PEI reagent (Polysciences). Two days post-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Cationic polyethyleneimine (PEI, Polysciences GmbH) solution was used to improve transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Transfection were performed with PEI MAX (#24765-1, Polysciences) or Lipofectamine 3000 (#L3000075 ...
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bioRxiv - Synthetic Biology 2024Quote: A 1 mg/mL solution of PEI Max (Polysciences, catalog no. 24765) was prepared for transient transfection as follows ...
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bioRxiv - Synthetic Biology 2024Quote: ... HEK293T cells were cultured in T25 flasks and transfected at ∼70% confluency with 2.5 μg of the pCDH lentiviral transfer vector of interest and packaging plasmids psPAX2 (1.25 μg) and pMD2.g (1.25 μg) with 25 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences). Approximately 72 hours post-transfection ...
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bioRxiv - Systems Biology 2024Quote: To prepare lectin-coated concanavalin beads (Polysciences), 25 uL of beads per 100,000 nuclei in each aliquot were combined with 850 μL Binding buffer (20 mM HEPES ...
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bioRxiv - Microbiology 2024Quote: ... After 1-hour incubation at 37°C the treated and the untreated live HEK293 cells expressing HBc and the untransfected HEK293 cells were washed and fixed with 0.1 M sodium phosphate buffer pH 7.4 containing 4% paraformaldehyde (EM grade, Polysciences) and 1% sucrose for 10-20 min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... fresh protease inhibitors) and bound to lectin-coated concanavalin A magnetic beads (40 µL beads per 100,000 nuclei; Polysciences, Warrington, PA). Bead-bound nuclei were chelated with blocking buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2024Quote: ... and 3) Ad helper genes using polyethyleneimine (#23966-1, Polysciences, USA). Cells were harvested 72 hrs post transfection ...
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bioRxiv - Neuroscience 2024Quote: ... OBs from young and aged C57BL/6J and Mitfmi-vga9/mi-vga9 mice were dissected and placed in a Peel-A-Way Disposable Plastic Tissue Embedding Molds (Polysciences, Inc.) filled with 5% agarose (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Intraepithelial lymphocytes (IEL) were isolated from the supernatants by filtering through nylon wool (Polysciences) and discontinuous Percoll (GE Life Sciences ...
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bioRxiv - Microbiology 2024Quote: ... The RIG-I expression plasmid (pCAGGS-RIG-I) was transfected into wild-type or RIG-I KO 293T cells employing Polyethylenimine “Max” (PEI MAX; PolySciences, Warrington, PA, USA). The transfection of poly(I:C ...
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bioRxiv - Immunology 2024Quote: ... and mounted to a coverslip in Aqua Polymount (Polysciences, 18606-20). Imaging was accomplished on a Leica Stellaris 5 confocal microscope ...
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bioRxiv - Microbiology 2024Quote: ... Cells were transfected with a total of 22–25 μg DNA using PEImax (Polysciences, Inc.). Each batch of PEI was titered to identify the optimal ratio of DNA:PEI for transfection (which was typically close to 1:2) ...
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bioRxiv - Molecular Biology 2024Quote: HEK293T cells were transfected with 3 µg pcDNA3 HA-KBTBD4 plasmid and 3 µg pcDNA3 CoREST-FLAG (full-length or truncated) using PEI MAX transfection reagent (Polysciences) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... the day before transfection with polyethyleneimine (PEI-max, #24765-1 Polysciences Inc).
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bioRxiv - Molecular Biology 2024Quote: ... This construct was transiently expressed in cells using polyethylenimine (PEI, Polysciences) as a transfection agent (3 µl/DNA µg).
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed 3x in PBS and mounted in Mowiol-4-88 (PolySciences) mounting medium containing 1:1,000 DAPI (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: Transfection mixes were made containing DNA and polyethylenimine (PEI; Polysciences, 23966) in a ratio of 1:3 (μg DNA/μg PEI ...
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bioRxiv - Microbiology 2024Quote: ... HEK-293T cells and HEK-293F cells were cultured and transfected using either Lipofectamine 3000 (Thermo) or polyethylenimine (Polysciences) as previously described (30 ...
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bioRxiv - Microbiology 2024Quote: ... Linear poly(ethyleneimine) (PEI) (PolySciences, Warrington, PA) with a molecular weight of 25 kDa was dissolved in dH2O to 1 mM ...
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bioRxiv - Molecular Biology 2024Quote: HEK293T cells were transfected with 2 µg pcDNA3 HA-KBTBD4 plasmid (mutant or WT) and with or without 3 µg pcDNA3 CoREST-FLAG (full-length or truncated) using PEI MAX transfection reagent (Polysciences) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Transient DNA transfections were performed using linear poly(ethylenimine) (1 mg/mL of solution in 10 mM Tris-HCl, pH 6.8; Polysciences), Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were mounted with Aqua-Poly/Mount (Polysciences). Images were acquired with a Zeiss LSM 700 confocal scanning microscope.
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bioRxiv - Molecular Biology 2024Quote: HEK 293T cells were transfected with lentiviral constructs and packaging plasmids (psPAX2 and pMD2.G) using PEI transfection (Polysciences). Two days post-transfection ...
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bioRxiv - Bioengineering 2024Quote: ... 10 μL of 1 mg/mL polyethylenimine (PEI MAX®, Polysciences 24765) and 1500 ng (ALFAtag-G-CSFR ...
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bioRxiv - Neuroscience 2024Quote: ... retinas were washed 4 times with PBS for 5 minutes prior to whole mounting onto slides with fluorescent mounting medium (Polysciences, Cat#18606-20). Slides were allowed to dry prior to imaging ...
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bioRxiv - Neuroscience 2024Quote: ... Worms were immobilized by either using 20 mM levamisole in M9 or Polybead polystyrene microspheres (Polysciences) for experiments involving GABAergic stimulation ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were transiently transfected using PEI MAX (Polysciences) and supplemented with 6 g/L glucose (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and they were mounted with Aqua- Poly/Mount (Polysciences, 18606-20) and cover slipped ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Aqua-Poly/Mount was purchased from Polysciences (Warrington, PA). Sigma 1 Receptor mouse monoclonal antibody was obtained from Santa Cruz Biotechnology (Dallas ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... transfection grade (PEI 25K) was obtained from Polysciences (23966-1). The selection reagents blasticidin ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cells were the exposed to 0.5 µm latex beads (carboxylated surface, yellow green-labelled, from Polysciences excitation 488 nm emission 527/32 nm) for 3 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Transfection complexes were made using PEI (1:3 ratio of DNA:PEI) (Polysciences Inc, 23966). PEI:DNA complexes were allowed to form for 15 minutes and then 50 μL of OptiMEM (Thermo ...
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bioRxiv - Physiology 2024Quote: ... and cover slipped with an aqueous mounting medium (Aqua-Poly/Mount, Polysciences, Warrington, PA).
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bioRxiv - Physiology 2024Quote: ... and cover slipped with an aqueous mounting medium (Aqua-Poly/Mount, Polysciences, Warrington, PA).
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bioRxiv - Physiology 2024Quote: ... and cover slipped with an aqueous mounting medium (Aqua-Poly/Mount, Polysciences, Warrington, PA).
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bioRxiv - Physiology 2024Quote: ... and stained with Verhoeff–van Gieson for elastic fiber visualization (Polysciences, 25089-1), Von Kossa for calcification (Polysciences ...