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Citations for Polysciences :
151 - 200 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... the plasmid and helper plasmid were transfected into HEK293T cells using polyethylenimine (# 24765, Polysciences). The virus was harvested after 72 h and purified from benzonase-treated cell lysates via an iodixanol density gradient (OptiPrep ...
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bioRxiv - Systems Biology 2024Quote: ... Plain Poly Styrene Nanoparticles (PSNPs, ∼100 nm) were obtained from Polysciences (www.polysciences.com).
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bioRxiv - Synthetic Biology 2024Quote: ... cells have been transfected using a home-made solution of PEI (MW 25000, Polysciences, stock concentration 0,324 mg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were mounted on coverslip in chrome alum (gelatin/chromate) and covered with Mowiol (Polysciences Inc.). Images were acquired with an upright LSM 700 confocal microscope (Zeiss).
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bioRxiv - Neuroscience 2024Quote: ... All samples were post-fixed 1% osmium tetroxide (Polysciences, Cat. 23311-10, USA) for 2 h and dehydrated in graded concentration of alcohols ...
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bioRxiv - Neuroscience 2024Quote: ... 6.8 µg polyethylenimine (PEI, 25kDa linear, Polysciences, 23966) was added along with a total of 2 µg of DNA to a final volume of 100 µl of DMEM (without FBS) ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were mounted on slides using AquaMount (Polysciences).
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bioRxiv - Neuroscience 2024Quote: Animals were immobilized on a pad composed of 4% ultrapure agarose in H2O in 1-2 µL of 5 mM levamisole in H2O or in a suspension of 0.1 µm polystyrene beads (Polysciences) in H2O ...
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bioRxiv - Cell Biology 2024Quote: ... were introduced into the cells by polyethyleneimine-mediated transfection (Polysciences, 24765 Warrington, PA). Cells were harvested after 72 hours ...
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bioRxiv - Neuroscience 2024Quote: ... coverslips were mounted onto microscopy slides using AquaPoly/mount (Polysciences Inc.). Neurons with nuclear and cytoplasmic localization of MRTFA or MRTFB were counted using Zeiss LSM 5 Pascal microscope ...
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bioRxiv - Cell Biology 2024Quote: ... and is still available from Polysciences, Ladd ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were dissociated using 0.05% Trypsin with 0.53 mM EDTA (Wisent Inc., Montreal, Canada) and transiently transfected with receptor and biosensor DNA using 25 kDa linear polyethylenimine (Polysciences Inc., Warrington, PA, US) in Cellstar® PS 96-well cell culture plates (Greiner Bio-One ...
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bioRxiv - Neuroscience 2024Quote: ... in DMEM complete medium as described previously17 with the following modifications: a 3:1 ratio of 1 µg/µl polyethenylamine (PEI) (Linear, MW 25,000, Polysciences, 23966) to transfer DNA was used in place of TransIT Lenti Transfection Reagent ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 475 ng either of pCAG-hSTAT6 (VT→AA, Y→F) or pcDNA was transfected by using PEI MAX (Polysciences, Warrington, PA, USA) according to the manifacture’s guide ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 5 µg of transfer plasmid were transfected with polyethylenimine (8mg/mL sterlile water, Polysciences, Warrington, PA, USA). After 60–72 h of incubation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Polyethylenimine (PEI) (25kDa) was obtained from Polysciences Inc (PA ...
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bioRxiv - Bioengineering 2024Quote: ... Polystyrene Polybead® microspheres (Polysciences, Inc.) of six diameters ranging from 1 to 15 μm were used as a sample material of known physical dimensions for instrument calibration ...
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bioRxiv - Biochemistry 2024Quote: ... cells on every dish were transfected with 15 μg of pcDNA3-HA-BACH1 using the PEI MAX reagent (24765, Polysciences, Warrington, PA). At 48 h after transfection ...
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bioRxiv - Bioengineering 2024Quote: ... using PEI MAX (Polysciences, 49553-93-7). Plasmids were acquired through the NIH HIV Reagent Program ...
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bioRxiv - Bioengineering 2024Quote: ... When imaging fluorescent beads (Polysciences, Inc. Fluoresbrite PS microspheres) to assess the LSM spatial resolution ...
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bioRxiv - Bioengineering 2024Quote: ... cells were transfected with 0.5 μg of plasmid DNA per well using 2.5 µL of 1 mg mL−1 polyethylenimine (PEI) (Linear, MW 25,000, Polysciences Inc.). Twenty-four hours after transfection ...
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bioRxiv - Bioengineering 2024Quote: ... with polyethylenimine (PEI) (Polysciences). All R-1b variants were produced in pCAGGS plasmids encoding the R-1b protein ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... and 0.8 µg pAdDF6 using PEI MAX (Polysciences). At 6-18 hours after transfection ...
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bioRxiv - Biophysics 2024Quote: ... we diluted 1 μm anti-digoxigenin-coated polystyrene beads (Polysciences) in nucleosome dilution buffer and added it to the imaging chamber for 30 minutes ...
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bioRxiv - Physiology 2024Quote: ... For Picrosirius Red Staining we followed the manufacturer’s instructions (Polysciences, #24901). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... positioned in an embedding mold (18646A-1, Polysciences) containing 100% OCT ...
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bioRxiv - Microbiology 2024Quote: ... using polyethylenimine (PEI; Polysciences Inc.). At 24 h post transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... Warm cross-link reversal buffer (200 µl 800 mM Tris-HCl pH8.0) was added followed by addition of 6 µl of 1:10 Biomag amine paramagnetic beads (48 mg/ ml, Polysciences cat. no. 86001-10). Homogenization was repeated ...
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Aromatase in adipose tissue exerts an osteoprotective function in male mice via phosphate regulationbioRxiv - Physiology 2024Quote: ... The decalcified tibiae were immersed in a 1:1 mixture of 5% potassium dichromate and 2% osmium tetroxide (23310-10, Polysciences, Inc., USA) for 48 h and stained ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 12 µL of 100 mg/mL linear polyethyleneimine (PEI) at molecular weight 25 kDa (Polysciences, Warrington, PA, USA) into 38 µL of a 150 mM NaCl solution ...
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bioRxiv - Physiology 2024Quote: ... and then transfected with the CYP27C1 construct or a control construct (pCAG-dsRed-2A- GFP) using polyethylenimine (PEI, 23966-2, Polysciences, Warrington, PA). After 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... pCRV1-Gag-Pol119 and pHCMV-VSV-G120 using polyethylenimine (PEI, Polysciences) in a 1:3 μg DNA/ µl PEI ratio in non-supplemented DMEM ...
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bioRxiv - Cell Biology 2024Quote: Transfections for analysis by WB were performed by mixing DNA and polyethylenimine (PEI, Polysciences) in a 1:3 ratio (w/w ...
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bioRxiv - Cell Biology 2024Quote: ... Polybead® Polystyrene Sampler Kit (19822-1) was purchased from Polysciences. Anti-GFP (11814460001 ...
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bioRxiv - Cell Biology 2024Quote: ... and MEFs were transfected with plasmids by polyethyleneimine (Polysciences). HeLa cells were transfected with plasmids by Lipofectamine LTX reagent with Plus reagent (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... containing 1:10,000 Fluoresbrite YG miscrospheres 1µm (Polysciences) in 2.15mm diameter glass capillaries (Zeiss) ...
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bioRxiv - Developmental Biology 2024Quote: ... sections were mounted with Aqua-Poly/Mount (Polysciences, 18606) and imaged with an Olympus FV1000 confocal microscope using a 30x oil objective.
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bioRxiv - Cancer Biology 2024Quote: Cells were fixed in cold 2.5% glutaraldehyde (25% glutaraldehyde stock EM grade, Polysciences, 111-30-8) in 0.01 M PBS (Fisher) ...
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bioRxiv - Immunology 2024Quote: ... frozen sections were fixed and stained using picrosirius red (Polysciences). Sections were scanned using the Axioscan.Z1 microscope (Carl Zeiss ...
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bioRxiv - Cell Biology 2024Quote: ... to induce LD formation and then cells were transfected with 3 µg of plasmid DNA/mL using Polyethylenimine HCl MAX (Polysciences, Inc) following the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... we labeled circulating monocytes by injecting the mice retro-orbitally with 1 µm diameter Flouresbrite flash red plain microspheres beads (Polysciences Inc, Warrington, PA) diluted in sterile PBS 24 hours before harvesting ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 60mL ice-cold 4% paraformaldehyde (PFA) (ref # 00380-1, Polysciences, Warrington, PA) in 0.1 M phosphate buffer (80 mM Na2HPO4 and 19 mM NaH2PO4 ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... packaging vector psPAX2 and envelope vector pMD2.G with a ratio of 4:2:1 using PEI Max (Polysciences) according to standard protocols with a 3:1 PEI Max:DNA ratio for 6 to 9 h ...
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bioRxiv - Cell Biology 2024Quote: ... Linear polyethyleneimine 25K (PEI) was purchased from Polysciences. Mouse anti-HA antibody (cat ...
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bioRxiv - Genomics 2024Quote: ... Fluoresbrite carboxyl bright blue beads (1 µm, Polysciences Cat # 17458-10) were added to the media (300 beads per cell ...
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bioRxiv - Genetics 2024Quote: ... Polyethylenimine (PEI) (Polysciences, 24765-1) was used for transfection ...
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bioRxiv - Genomics 2024Quote: Lentivirus was produced via transfection of plasmids expressing RfxCas13d guide RNAs under the U6 promoter with lentiviral packaging plasmids using linear polyethylenimine (PEI) MW25000 (Polysciences 23966) in HEK293FT ...
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bioRxiv - Neuroscience 2024Quote: HEK293 or HEK293T cells were transfected with plasmids expressing GFP-fused NCAN using Lipofectamine 3000 (Thermo) or PEI Max (Polysciences). After three or four days ...
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bioRxiv - Molecular Biology 2024Quote: ... with modified pCW57.1 target vector in a ratio of 5 : 1 : 5 : 5 into HEK293 cells using polyethyleneimine (PEI, Polysciences, 23966). Three days post-transfection ...
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bioRxiv - Systems Biology 2024Quote: ... A Per2 plasmid was transfected with polyethylenimine (Polysciences, #233966-100) using Opti-MEM (Gibco ...