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Citations for Polysciences :
151 - 200 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Twenty-four hours after transfection with 0.5 μg of plasmid DNA per well using 2.5 µL of 1 mg mL−1 polyethylenimine (PEI) (Linear, MW 25,000, Polysciences Inc.), cells were incubated in HBSS containing 10 mM HEPES-NaOH (pH 7.4 ...
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bioRxiv - Bioengineering 2024Quote: ... transfection was carried out using 0.5 μg of plasmid DNA and 2.5 µL of 1 mg mL−1 polyethylenimine (PEI) (Linear, MW 25,000, Polysciences Inc.). Twenty-four hours after transfection ...
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bioRxiv - Bioengineering 2024Quote: ... and delivered to HEK293T cells by transient transfection using linear 25 kDa polyethyleneimine (Polysciences). About 24 h post-transfection ...
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bioRxiv - Bioengineering 2024Quote: ... 485 μL of linear polyethyleneimine (Polysciences, 2.58 mg/mL, 25 kDa) was mixed with 115 μL of 150 mM sterile filtered sodium chloride solution ...
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bioRxiv - Bioengineering 2024Quote: ... 150,000 cells/mL with 1 v/V% 500 nm fluorescent beads (Floresbrite YG carboxylate beads, Polysciences, Inc.) were encapsulated within each hydrogel on a Nunc glass bottom dish (Nunc Glass ...
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bioRxiv - Bioengineering 2024Quote: ... 174 μl (1 μg/ul) polyethylenimine (PEI, Polysciences, 49553-93-7) was vortex mixed with 1930 μl Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and BT-474 were transfected using PEI MAX 40K (Polysciences). For prime editing with the pCMV-PE5max-SF3B1-K700E all-in-one construct ...
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bioRxiv - Cancer Biology 2024Quote: ... and transfected using PEI MAX 40K (Polysciences). For Cas9 and AAV HDR experiments ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA was added to 200 μl of DMEM and mixed with 7.5 μl of PEI reagent (Polysciences Inc cat# 23966). Tubes were incubated at room temperature for 15 min before adding to cells ...
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bioRxiv - Cancer Biology 2024Quote: ... TRAP staining was performed after fixation with 4% paraformaldehyde (Polysciences, Warrington, PA) and 0.1% Triton X-100 in PBS according to the manufacturer’s instructions (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: Spheroids in the Elplasia plate were then placed on ice and fixed in situ for 20 minutes on ice in 1% effective Formaldehyde added to the serum-free media (Formaldehyde, 16%, methanol-free, Ultra Pure; Polysciences #18814-20). The cell-fixation media was then removed and the cells were incubated with 50 mM TrisHCL (Thermo Scientific #J22638.AE ...
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bioRxiv - Cancer Biology 2024Quote: ... packaging plasmid using polyethylenimine (Polysciences). Sodium butyrate (Sigma Alrich ...
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bioRxiv - Cancer Biology 2024Quote: ... 293T cells were co-transfected with LentiCRISPR v2 plasmids along with packaging plasmids psPAX2 and pMD2.G using PEI transfection reagent (#23966-100, Polysciences). Lentiviruses were collected 48 hours after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... and mounted with an aqueous mounting medium (Aqua-Poly/Mount; Polysciences).
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bioRxiv - Biophysics 2024Quote: ... for 10 min followed by 0.1 % glutaraldehyde (GTA, a collagen crosslinker; Polysciences, Inc.) for 20 min ...
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bioRxiv - Biophysics 2024Quote: ... Cells were transfected using a polyethylenimine (PEI) agent (10 μl of 1 mg/ml per dish hereafter; Polysciences) with pCAG plasmids encoding human A1R ...
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bioRxiv - Biophysics 2024Quote: ... Polystyrene beads of 1 µm diameter (Polysciences inc ...
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bioRxiv - Cell Biology 2024Quote: ... L4 stage larvae were immobilized in 3.5 µl polystyrene nanobead suspension (2.5% by volume, 0.1 µm diameter; Polysciences, Warrington, PA) sandwiched between a coverslip and a 10% agarose pad ...
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bioRxiv - Cancer Biology 2024Quote: ... after which they were collected in Aqua-Poly/Mount (18606-20, Polysciences) and transferred to slides ...
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bioRxiv - Cell Biology 2024Quote: ... Picrosirius red staining (Polysciences, 24901) was used to measure collagen fibril thickness ...
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bioRxiv - Cell Biology 2024Quote: Equal numbers of HEK293T cells were seeded Wnt7a plasmid was transfected with linear polyethylenimine (Polysciences), accordingly to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentivirus particles were produced in HEK293T cells after co-transfection of lentivirus plasmid vectors with packaging plasmids (VSVG and lenti gag/pol) using polyethylenimine (PEI; NC1014320; Polysciences, Warrington, PA, USA). After 48 h and 72 h ...
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bioRxiv - Biochemistry 2024Quote: ... cells on every dish were transfected with 15 μg of pcDNA3-HA-BACH1 using the PEI MAX reagent (24765, Polysciences, Warrington, PA). At 48 h after transfection ...
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bioRxiv - Bioengineering 2024Quote: ... cells were transfected with 0.5 μg of plasmid DNA per well using 2.5 µL of 1 mg mL−1 polyethylenimine (PEI) (Linear, MW 25,000, Polysciences Inc.). Twenty-four hours after transfection ...
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bioRxiv - Bioengineering 2024Quote: ... with polyethylenimine (PEI) (Polysciences). All R-1b variants were produced in pCAGGS plasmids encoding the R-1b protein ...
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bioRxiv - Bioengineering 2024Quote: ... using PEI MAX (Polysciences, 49553-93-7). Plasmids were acquired through the NIH HIV Reagent Program ...
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bioRxiv - Bioengineering 2024Quote: ... When imaging fluorescent beads (Polysciences, Inc. Fluoresbrite PS microspheres) to assess the LSM spatial resolution ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... and 0.8 µg pAdDF6 using PEI MAX (Polysciences). At 6-18 hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were mounted using Aqua-Poly/Mount (Polysciences cat: 18606) and cover slipped (coverslips of #1.5 thickness) ...
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bioRxiv - Neuroscience 2024Quote: ... bEnd.3 cells were fixed in 4% cold PFA (Polysciences, Inc. PA, USA), and the immunofluorescence staining was performed following the same procedures as stated above.
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bioRxiv - Neuroscience 2024Quote: ... The cell pellets were fixed in cold 4% paraformaldehyde (PFA) (Polysciences, Inc. Warrington, PA, USA) at room temperature for 20 min and washed with staining medium (PBS containing 0.1% NaN3 and 2% FCS) ...
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bioRxiv - Neuroscience 2024Quote: ... HEK cell transfections were performed using the polyethylenimine (PEI) transfection reagent (Polysciences; cat. #23966) with a 3:1 PEI- to-DNA ratio when the cells reached 70% confluency ...
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bioRxiv - Neuroscience 2024Quote: ... The dyes injected included fluorescent and other tracers: Fast Blue (FB, case AT, 3% dilution, 2 µl; Polysciences), Diamidino Yellow (DY ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293T cells at 70% confluency in 15-cm culture plates were transfected with pFUW-GFP-AR, along with viral packaging and envelope proteins (pRSV/REV, pMDLg/RRE, and pVSV-G) using polyethylenimine transfection reagent (Polysciences; cat. #23966). 48 h post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... DAPI was added to the PBS at 1:2500 for nuclear staining) and then mounted on charged glass-slides and cover-slipped in Aqua Poly/Mount (Polysciences, Inc.). Primary antibodies used ...
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bioRxiv - Neuroscience 2024Quote: ... with PEI Max (Polysciences, Cat # 24765) in HEK293T cells ...
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bioRxiv - Neuroscience 2024Quote: ... Sections stained with antibodies against MBP or pMLC2/Pan-NF were placed in Liberate Antibody Binding (L.A.B. Polysciences Inc.), after the initial PBS wash ...
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bioRxiv - Neuroscience 2024Quote: Cells were transfected with Linear Polyethylenimine 25.000 (PEI) (Polysciences, Inc., USA) or Fugene HD reagent (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... which was then supplemented with 150 µl of 1 mg/mL Polyethylenimine (PEI; Polysciences Cat#23966), incubated for 10 minutes and then added to a single 15 cm plate of fully confluent cells ...
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bioRxiv - Neuroscience 2024Quote: ... A minimum of 4 weeks after viral injections fluorophore-conjugated retrograde tracers (Dextran 10,000MW, AlexaFluor 488 and 568, Invitrogen; FastBlue, Polysciences) were injected bilaterally into Area X ...
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bioRxiv - Neuroscience 2024Quote: ... 5 days after having injected HVC with the retrograde tracer Fast Blue (150nl/HVC, Polysciences) the brain was perfused with 4% paraformaldehyde (PFA) ...
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bioRxiv - Neuroscience 2024Quote: ... using polystyrene beads (Polysciences, 00876-15, 0.1 μM diameter) to immobilize the animals (Kim et al. ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293FT cells were seeded on 15-cm dishes without antibiotics for close to 24 hours and co-transfected with the following plasmids using Polyethylenimine (100 μg/dish, Polysciences, #23966-1): pHGTI-Adeno1 helper (22 μg/dish) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were mounted in Aqua-Poly/Mount (Polysciences, Warrington, PA, USA). Photomicrographs for further analysis were taken with an inverted fluorescence microscope (Leica DMi 8 ...
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bioRxiv - Neuroscience 2024Quote: ... the left sides of the brains were placed (anterior portion facing downwards) in disposable cryomold (Polysciences Inc. #18986– 1), and frozen in O.C.T ...
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bioRxiv - Neuroscience 2024Quote: ... Dehydration using a graded ethanol series followed by propylene oxide incubation and resin infiltration with Polybed812 epoxy resin (Polysciences). Blocks were polymerized for 48 hours at 60°C.
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bioRxiv - Neuroscience 2024Quote: ... and a rep/capsid plasmid using Polyethylenimine “Max” (24765-1; Polysciences, Inc., Warrington, PA, USA). Viral particles were harvested from the culture medium 6 days after transfection and were concentrated by precipitation with 8% polyethylene glycol 8000 (Merck ...
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bioRxiv - Neuroscience 2024Quote: ... the transfer and pDGM6 packaging plasmids were co-transfected in 293T cells (ATCC) using PEI reagent (Polysciences, 23966-1), as described previously 52 ...
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A conserved RNA switch for acetylcholine receptor clustering at neuromuscular junctions in chordatesbioRxiv - Developmental Biology 2024Quote: ... and empty vector was used to transfect each of 6 well plate(s) and 7.5 µL of linear polyethylenimine (PEI; Polysciences), MW 25,000 (1mg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were fixed with 4% paraformaldehyde (Polysciences) for 10 min ...