Labshake search
Citations for Polysciences :
2551 - 2600 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... 1 L of cells at 1×106 cells were transfected with complexes composed of 250 μg of plasmid DNA and 750 μg of polyethylenimine (Polysciences, Cat. # 24765-1). For the expression involving kifunensine ...
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bioRxiv - Biophysics 2020Quote: ... Beaded cantilevers were generated by first drying 6.0 μm carboxylate beads onto a coverslip (17141-5, Polysciences, Inc); a small amount of UV-curable glue (NOA81 ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected using PEI-max (#24765-1, Polysciences) with 100 ng of pSNL-N2-C or pSNL-N2-Muc7 and 100 ng of pSNL-N1-”C interactors” ...
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bioRxiv - Microbiology 2020Quote: ... transfections were done in T-75 flasks using polyethylenimine (Polysciences Inc, PA, USA). Transfections were done according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... were transfected with the plasmid DNA diluted in PBS (0.67 μg total plasmid DNA per ml of culture) using polyethylenimine (Polysciences, Inc.) at a DNA-to-PEI ratio of 1:3 ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293FT cells were transfected with the lentiviral vector pCLTs together with the packaging plasmids pCMV-VSVG-RSV-Rev (addgene) and pCAG-HIVgp (addgene) at a 1:0.5:0.5 ratio using PEI Max reagent (Polysciences) according to the Manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 4:2:1 using polyethylenimine (PEI) (Cat#23966-1, Polysciences, Inc.) (PEI:DNA mass ratio 3:1) ...
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bioRxiv - Cell Biology 2020Quote: ... Freeze-substitution and Lowicryl HM20 (Polysciences, Inc.) resin-embedding were done as previously described in (Kukulski et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Polyetheleneimine reagent (Polysciences, Inc.), Radioactive ATP (BRIT ...
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bioRxiv - Cell Biology 2020Quote: RPE1 cells were transfected with PX458 vector with specific BBS sgRNA using polyethylenimine (Polysciences, Inc., 23966-2). After 48 h ...
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bioRxiv - Cell Biology 2020Quote: ... Silica 100-nm microspheres (Polysciences, Warrington, PA) were analyzed to check instrument performance and determine the viscosity coefficient of B88 ...
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bioRxiv - Cell Biology 2020Quote: ... or polyethylenimine (PEI-MAX, Polysciences) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... the coverslips were mounted onto glass slides with Aqua-Poly/Mount (Cat No. 18606, Polysciences Inc). Slides were left to cure overnight at RT prior to imaging ...
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bioRxiv - Cell Biology 2020Quote: ... The Ecad and Dsc3 plasmids were transiently transfected into HEK 293T cells using Polyethylenimine (PEI) (Polysciences, Inc.). Four days post transfection ...
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bioRxiv - Cell Biology 2020Quote: ... various stages of the transgenic animals were mounted on 5 % agar pads with polystyrene nanoparticles (Polysciences, 2.5 % by volume) as previously described43 and imaged at an AxioImager M.2 fluorescence microscope (Zeiss ...
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bioRxiv - Cell Biology 2020Quote: ... from Bangs Laboratories and Latex polymer 3-micron beads (17134-15) from Polysciences. Murine IFNγ (315-05 ...
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bioRxiv - Cell Biology 2020Quote: ... 3-micron beads (Polysciences Inc) were resuspended in 0.1 M Borate and incubated with human IgG at 4°C overnight while rotating ...
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bioRxiv - Cell Biology 2020Quote: ... PlatE cells were co-transfected with the packaging plasmid pMRX-puro-tfLC3 plasmid and the envelope-encoding plasmid pVSVG by using of polyethylenimine (Polysciences Inc.). Supernatants were harvested 48 h post-transfection and filtered.
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were transfected using either PEI Max (Polysciences) or the calcium phosphate method ...
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bioRxiv - Cell Biology 2020Quote: ... MLV-GagPol/pHIV 8.1 and pHIT VSVg at a ratio of 2:3:1 for 48 h using polyethylenimine (Polysciences, Germany). 293T supernatant was collected and filtered through a 0.2-μm filter before being used to transduce MDCK II cells ...
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bioRxiv - Cell Biology 2020Quote: ... and 15µg of an AAV plasmid carrying the transgene of interest were mixed in OptiMEM with PEI-MAX (final concentration 80µg/ml, Polysciences #24765). 2ml of this solution were then added dropwise to each of the 6 HEK293T 15cm2 plates ...
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bioRxiv - Cell Biology 2020Quote: ... or chTOGKK/AA-3Flag (pSB2977) using 85 μg of polyethyleneimine (PEI, Polysciences 23966-1) as previously reported (Longo ...
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bioRxiv - Cell Biology 2020Quote: ... The TEM samples were prepared by dropping 5 μl of solution onto a 400-mesh carbon film-coated copper grids (24945-50, Polysciences, Inc., PA, USA), which were glow-discharged by a plasma cleaner ...
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bioRxiv - Cell Biology 2020Quote: ... and 20 μl polyethylenimine (PEI; 1 mg/ml) (Polysciences) was added ...
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bioRxiv - Cell Biology 2020Quote: ... and 0.05 μm Polybead microspheres (Polysciences) and only first ovulations were imaged ...
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bioRxiv - Cell Biology 2020Quote: ... Embryos were mounted on glass slides in Aquapolymount (Polysciences, Inc). Primary and secondary antibodies were ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were dehydrated in graded ethanol solutions and infiltrated with graded epoxy resin (Poly/Bed 812, Polysciences, Warrington, PA) / propylene oxide for 14 days before polymerization at 60 °C.
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bioRxiv - Cell Biology 2020Quote: ... washed with PBS and stained twice with a 4% hydochloric acid and 4% potassium ferrocyanide (1:1 v/v) solution of for 25 minutes (Polysciences Prussian Blue stain kit, PA USA). After washing with PBS ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were isolated from Drosophila melanogaster adult females straight into fixation medium and fixed in 4% PFA (prepared fresh from a stock solution of 16% paraformaldehyde, methanol free ultra - pure EM grade, Polysciences) in Cytoskeleton Buffer [21] ...
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bioRxiv - Cell Biology 2020Quote: Third-generation VSV.G pseudotyped HIV-1 lentiviruses were produced by polyethylenimine (PEI, Polysciences #23966-2)-transfection of HEK293T cells and packaged with VSVG-coats using established methods ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 and U2OS cells were transiently transfected using PEI-Max Transfection reagent (Polysciences, 24765) and optimem (gibco ...
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bioRxiv - Cell Biology 2020Quote: ... dehydrated in graded ethanol series and embedded with Epon-filled BEEM capsules (EMS; Polysciences, Inc., 00224) placed on top of the Mattek dish ...
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bioRxiv - Cell Biology 2020Quote: ... Following transfection using polyethyleneimine (Polysciences #24765-1), virus-containing supernatant was collected 48 h later and filtered through a 0.45 μm polyethersulfone filter (VWR 28145-505) ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were then embedded in Lowicryl HM-20 (Polysciences Inc.) and cut into 70 ...
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bioRxiv - Cell Biology 2020Quote: ... embedded in Lowicryl HM-20 (Polysciences Inc.) (Kukulski et al. ...
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bioRxiv - Cell Biology 2020Quote: For virus production, lentiCRISPR v2 plasmids(Sanjana et al, 2014) were transfected using polyethylenimine (Polysciences) into 293T cells along with psPAX and pMD2.G packaging plasmids (Addgene ...
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bioRxiv - Bioengineering 2020Quote: ... The nanoparticles were then formed by coating the PLGA nanoparticles with branched polyethylenimine (Polysciences, MW=70,000, Warrington, PA) through reaction with EDC and sulfo-NHS as described in previous work.31 The nanoparticles were washed once with 1 M NaCl solution and once with DI H2O by ultracentrifugation for 20 minutes ...
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bioRxiv - Cell Biology 2020Quote: Cells at 30-40% confluency were transfected with 2µg DNA in six well plates using polyethylenimine (PEI; #23966, Polysciences Inc.). pcDNA3.1 or pBlueScript were used as empty vector controls ...
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bioRxiv - Microbiology 2020Quote: ... infected cells then were fixed in freshly prepared mixture of 4% paraformaldehyde and 0.05% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM PIPES/0.5 mM MgCl2 buffer (pH 7.2 ...
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bioRxiv - Evolutionary Biology 2020Quote: Transfection of HEK293T cells was carried out using polyethylenimine (Polysciences Inc.). We used a second-generation packaging system to generate the lentiviruses used in this study (gift from Connie Cepko Harvard Medical School) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Transfection of HEK293T cells was carried out using polyethylenimine (Polysciences Inc.). Following concentration ...
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bioRxiv - Developmental Biology 2020Quote: ... Sectioning was performed as described above and sections were covered with Aqua-poly/mount (Polysciences). Images were obtained using a Zeiss AxioObserver inverted microscope equipped with Zen ...
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bioRxiv - Developmental Biology 2020Quote: ... embedded in JB4 resin (Polysciences, Inc.) and sectioned (10um ...
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bioRxiv - Developmental Biology 2020Quote: ... For imaging and quantification immunohistochemistry brain slices mounted in Aqua-Poly/Mount (Cat No. 18606-20, Polysciences, Inc) with #1.5 thickness coverslips were imaged with a laser scanning confocal microscope Leica TCS SP5 ...
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bioRxiv - Developmental Biology 2020Quote: ... once in PBS for 10 min and mounted in Aqua/Poly mount (Polysciences).
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bioRxiv - Developmental Biology 2020Quote: ... and stained with Alcian blue (Polysciences,Warrington, PA, USA) for GAG detection ...
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bioRxiv - Developmental Biology 2020Quote: ... High resolution episcopic microscopy (HREM) was performed as previously described97 using an JB-4 embedding kit (00226-1, Polysciences GMBH, Germany). μMRI and HREM data were analysed using OsiriX MD DICOM viewer version 9.0.2 (Pixmeo) ...
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bioRxiv - Developmental Biology 2020Quote: ... Fixed and stained embryos were mounted in Aqua Polymount (Polysciences).
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bioRxiv - Developmental Biology 2020Quote: ... dehydrated in ethanol and embedded in epoxy resin poly/Bed 812 (Polysciences Inc., Warrington, Pennsylvania). Subsequently ...
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bioRxiv - Developmental Biology 2020Quote: ... These vectors were transfected into HEK293T cells with PEI MAX (Polysciences Inc., Warrington, Pennsylvania) according to the manufacturer’s protocol ...