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Citations for Polysciences :
2401 - 2450 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: WT HEK293T cells were transfected with polyethylenimine (Polysciences) using a DNA to polyethylenimine ratio of 1:2.5 ...
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bioRxiv - Neuroscience 2021Quote: ... washed in PBS and mounted onto glass slides using fluorescence mounting medium Aqua-Poly/Mount (Polysciences). 100 randomly selected BrdU-positive cells from each animal along the rostro-caudal axis of the DG were imaged at 400β magnification with spectral confocal microscope (TCS SP2 ...
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bioRxiv - Neuroscience 2021Quote: ... diamidino yellow dihydrochloride (DY, Polysciences) and fast blue (FB ...
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bioRxiv - Neuroscience 2021Quote: ... and fast blue (FB, Polysciences). BDA and FE resulted in bidirectional transport ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed using an osmotically balanced and methanol-free 4 % formaldehyde (Polysciences) solution which has previously been shown to not cause detectable nuclear shrinkage (59) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with plasmids using polyethylenimine "Max" (Polysciences, USA) with a standard method.
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bioRxiv - Neuroscience 2021Quote: ... embedded in OCT compound (Polysciences), and frozen sections were prepared at 14 μm for immunohistochemistry or 18 μm for RNA in situ hybridization as previously described (John et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Silica 100-nm microspheres (Polysciences, Warrington, PA) served as a control to check instrument performance ...
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bioRxiv - Microbiology 2021Quote: A pME18S expression plasmid containing the full-length or subunit spike protein was transiently transfected into HEK293T cells using PEI max (Polysciences); the pMx-GFP expression plasmid was used as the marker of transfected cells.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To generate pseudotyped reporter virus the 293T cells were transfected with 4 plasmids mixed with Polyethylenimine (PEI, Polysciences): dual reporter genome vector ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were mounted in Aqua-Poly/Mount (PolySciences) before being imaged on a Leica TCS SP5 X (Swarthmore ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid DNA (1 mg) was mixed by dropwise addition of 2 mg linear polyethylenimine MW 25000 (Polysciences Inc, Warrington USA) in 25 ml Gibco Hybridoma-SFM (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... cells were transfected using polyethylenimine (PEI) 25-kDa linear polymer (Polysciences Corporation Ltd. #23966) or Effectene Transfection reagent (Qiagen #301425 ...
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bioRxiv - Biophysics 2021Quote: ... at a density of 5-7 million cells and transfected the next day with PEI (Polysciences, Germany) and 16:4:4:8 μg DNA encoding hASIC1a TAG variants ...
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bioRxiv - Biochemistry 2021Quote: ... and Firefly-luciferase as a control) using PEI (Polysciences #24765). Cells were harvested 24 h post plasmid transfection in 200 μL lysis buffer (60 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with plasmid DNA and 25 kDa polyethyleneimine (Polysciences, PA) at 1 μg/mL in Freestyle 293 media ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells mL-1 culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa polyethylenimine (Polysciences Inc., Germany) per mL culture volume ...
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bioRxiv - Biochemistry 2021Quote: ... Transient transfections were performed 24 h prior to cell lysis using polyethylenimine PEIMax (0.1% w/v) (Polysciences)[39] ...
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bioRxiv - Biochemistry 2021Quote: ... 4 ml (from 1 mg/ml stock) of linear polyethyleneimine (PEI) MAX (Mw 40,000; Polysciences Inc.) was diluted in the same medium to a volume of 30 ml in a separate tube and mixed ...
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bioRxiv - Biochemistry 2021Quote: ... and AceTAG lentiviral plasmids using PEI (Polysciences Inc). Viral particles were collected 72 h after transfection and filtered through a 0.45 μM membrane ...
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bioRxiv - Biochemistry 2021Quote: The expression vectors encoding for all S proteins were transiently transfected into HEK293 Freestyle (HEK293F) cells with polyethylenimine (PEI, linear, 25 kDa, Polysciences, USA) at a ratio of DNA ...
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bioRxiv - Microbiology 2021Quote: ... All transfections were performed using polyethylenimine (PEI; Polysciences) at a 1:3 ratio (mg DNA/mg PEI ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293F cells were transfected using PEI 25K linear transfection reagent (Polysciences). Expression was performed at 37°C for 7 hours followed by a second expression phase at 30°C for 72 hours.
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bioRxiv - Microbiology 2021Quote: ... and inverted onto 5 µL of Aqua-Poly/Mount (Polysciences, Warrington, PA) on a slide ...
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bioRxiv - Microbiology 2021Quote: ... using PEI-Max MW 40,000 (PolySciences, CAS Number: 49553-93-7) mixed with 600 ng of PsPax2 (Addgene # 12260) ...
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bioRxiv - Developmental Biology 2021Quote: ... and fixed in 4% formaldehyde (Polysciences, Inc., 18814-10) in 1xPBS for 30 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were mounted on glass slides containing Aqua Polymount (Polysciences). An A1 Nikon Confocal microscope with a 40× oil objective ...
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bioRxiv - Developmental Biology 2021Quote: ... worms were immobilized using 100nm polystyrene beads (Polysciences cat. #64010). Regions of interest were selected with the freehand ROI tool ...
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bioRxiv - Developmental Biology 2021Quote: ... where worms were immobilized using 100nm polystyrene beads (Polysciences cat. #64010). For lateral images of B-LINK components we acquired z-stacks at 0.37 μm intervals spanning the entirety of the B-LINK ...
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bioRxiv - Synthetic Biology 2021Quote: ... HEK293T were transfected with Polyethylenimine (PEI) (Mw 40000; Polysciences, Inc.) using a ratio of 1:3 (μg DNA to μg PEI ...
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bioRxiv - Systems Biology 2021Quote: Stable reporter cell lines were transfected with synTF plasmid constructs using polyethylenimine (PEI, Polysciences) as described in (Park et al. ...
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bioRxiv - Systems Biology 2021Quote: ... we added 80 μL of polyethylenimine (Polysciences, cat. 23966) to 500 μL of Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... were transiently transfected at a cell density of 1 × 106 cells/mL using a 1:3 ratio of plasmid DNA and polyethylenimine (PEI 25K™, Polysciences). Cells were incubated at 37°C for 7 days in a humidified incubator containing 8% CO2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The transfection solution was prepared using Polyethylenimine (PEI) MAX (MW 40000; Polysciences, Inc.) at a 1:3 (μg DNA to μg PEI ...
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bioRxiv - Bioengineering 2021Quote: ... 1 mg/mL plasmid DNA was mixed with 2 mg/mL linear 25 kDa polyethyleneimine (Polysciences) and transfected in OptiPRO SFM medium (4% final volume) ...
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bioRxiv - Genomics 2021Quote: ... Immunostained cells were mounted in Aqua-Poly/Mount (Polysciences) and analyzed at epi-fluorescent or confocal microscopy ...
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bioRxiv - Biochemistry 2021Quote: ... Transfection reactions were prepared by the addition of 2-10 μg of bacmid DNA to 100 μL 1xPBS and 12 μL polyethyleneimine (PEI) at 1 mg/mL (Polyethyleneimine ‘‘Max’’ MW 40.000, Polysciences #24765, USA) to 100 μL 1xPBS ...
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bioRxiv - Cell Biology 2021Quote: ... about 1.5 mg of the plasmid was premixed with 3 mg of polyethylenimines (PEIs) (Polysciences) in 50 mL of fresh medium for 15 mins before adding to cell culture ...
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bioRxiv - Neuroscience 2021Quote: ... slices were mounted with Aqua-Poly/Mount (18606-20, Polysciences). A subset of slices was co-stained with mouse anti-NeuN (1:300 ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips with the hippocampal cultures were mounted with Mowiol 4–88 antifade medium (Polysciences Europe). Neuronal images were acquired using an Olympus IX81 inverted epifluorescence microscope at 63× optical magnification with a CCD camera (Princeton MicroMax ...
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bioRxiv - Neuroscience 2021Quote: ... plasmids for these proteins were co-transfected with AnkR-GFP in HEK293T cells using PEI Max (Polysciences, 24765) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and mounted on slides with Aqua-Poly/Mount (Polysciences) for confocal imaging.
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bioRxiv - Neuroscience 2021Quote: ... Freeze substitution and Lowicryl HM20 (Polysciences, Inc.) resin embedding were performed as previously described [56] ...
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bioRxiv - Genetics 2021Quote: ... washed with 1X PBS and mounted in Poly aquamount (Polysciences). The following primary antibodies were used ...
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bioRxiv - Neuroscience 2021Quote: ... samples were covered with Mowiol (Polysciences, Warrington, PA, USA) containing DABCO (1,4-diazo-cyclo[2.2.2] octane ...
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bioRxiv - Developmental Biology 2021Quote: Fluorescent beads (Fluoresbrite multifluorescent 0.5-μm beads 24054, Polysciences Inc.) are diluted 1:1,000 in 1% low-melting point agarose solution into which the embryo is mounted for imaging ...
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bioRxiv - Neuroscience 2021Quote: ... adult hermaphrodite worms were immobilized on 10% agarose pads under a coverslip using the friction produced by 0.10 µm diameter polystyrene beads (Polysciences, Inc. Catalog number 00876) and imaged using an inverted microscope (Axiovert S100 ...
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bioRxiv - Neuroscience 2021Quote: ... worms were fed a 50% dilution of a 2.5% weight by volume (4.55 x 1010 particles per mL) solution of 1 µm diameter polystyrene beads (Polysciences, Inc ...
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bioRxiv - Physiology 2021Quote: ... ATP [5mM] and 4% formaldehyde (Polysciences). After fixation ...
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bioRxiv - Microbiology 2021Quote: ... With 210 nm yellow green fluorescence beads (17151-10, Polysciences, USA), the spatial and axial resolution of non-degenerate (ND)-TPEF ...