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Citations for Polysciences :
2701 - 2750 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and MERS CoV lacking the endoplasmic retention signal sequence were cloned into pCAGGS expression system (pCAGGS-SARS2-S, pCAGGS-SARS-S, and pCAGGS-MERS-S) and were transfected using Polyethylenimine (PEI) (Polysciences, USA) at 1:3 ratio in HEK293T cells ...
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bioRxiv - Microbiology 2020Quote: ... Transfection complexes were prepared by mixing plasmid DNA with polyethyleneimine (PEI Max 40k, Polysciences Inc., Warrington, PA) in a 1:4 mass ratio ...
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bioRxiv - Biophysics 2020Quote: Unmodified 560 nm diameter polystyrene beads (Polysciences) were mixed with purified recombinant full-length Drosophila kinesin-1 (12) ...
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bioRxiv - Neuroscience 2020Quote: ... tissue samples were placed into embedding moulds (Polysciences, T-8), filled with warm gelatine (20% ...
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bioRxiv - Neuroscience 2020Quote: ... Mounted slices were left to dehydrate overnight before applying coverslips using either Aqua-Poly/Mount (Polysciences Inc., Germany) or Fluoroshield with DAPI (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... dried and coverslipped with Aqua Polymount (Polysciences Inc., Warrington, PA).
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed in PBS and mounted with Aquapolymount (Polysciences).
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bioRxiv - Neuroscience 2020Quote: ... All other AAVs were prepared in large scale HEK293T cell cultures that were transfected with mixture plasmids of AAV encoding full-length cDNAs and the serotype DJ packaging plasmids pHelper and pRC-DJ with polyethyleneimine (Polysciences). 48 h after transfection ...
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bioRxiv - Neuroscience 2020Quote: ... brain organoids were placed directly in 4% methanol-free formaldehyde (Polysciences) diluted in DPBS (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Fast blue (Bentivoglio et al., 1980; Kuypers et al., 1980) (Polysciences, Pennsylvania) was prepared by dissolving 1mg of powder in 30μL 1X phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: ... L4 animals were immobilized using 0.05 µm polystyrene beads (Polybead Microspheres, Polysciences Cat #08691-10) or in 0.2mM Levamisole (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... and were transfected using linear polyethylenimine (MW 25,000; Polysciences). SRE-Luciferase Reporter Assay was performed using the Dual-Luciferase assay system (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... Males were placed on a 10% agar pad and immobilized with 0.01 µm latex beads (Polysciences, Inc). Target neurons were identified based on the expression of fluorescent markers ...
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bioRxiv - Neuroscience 2020Quote: ... with Aqua-Poly/Mount (Polysciences, Inc., Germany). Fluorescent pictures of TH labelling and mCherry expression were taken using a slide scanner (Z1 Axioscan ...
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bioRxiv - Neuroscience 2020Quote: ... and embedded in Poly/Bed® 812 (Polysciences Europe GmbH ...
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bioRxiv - Neuroscience 2020Quote: ... UK) and the left tibia was injected with 5 μl of 4% Fast Blue neuronal tracer (Polysciences Inc., Germany). After a 7-day recovery period ...
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bioRxiv - Neuroscience 2020Quote: ... using aqueous PolyMount™ (#18606, Polysciences) or Mowiol™ (AppliChem ...
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bioRxiv - Microbiology 2020Quote: ... or PEI MAX40 (Polysciences 24765-1) following the manufacturers’ recommendation.
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bioRxiv - Molecular Biology 2020Quote: ... YFP-TOP2B was transfected into HEK293F cells in Hyclone TransFx media (Cytiva) using PEI (Polysciences) and purified using the YFP-tag system (Schellenberg et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... sections were rehydrated and stained for collagen using a Picro Sirius red staining kit (PolySciences). After staining ...
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bioRxiv - Neuroscience 2020Quote: ... One-day-old adults from these strains were mounted on a 10% agarose pad with 1.6 µL of a mixture containing equal measures of polystyrene beads (Polybead, Polysciences Inc.) and 30 mM muscimol (MP Biomedicals) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were mounted on slides with Aqua Polymount (Polysciences). Images were acquired using either an epifluorescence microscope (Zeiss Axio-imager M1 ...
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bioRxiv - Plant Biology 2020Quote: ... the sections were mounted with Poly-Mount® (Polysciences, Inc.) and observed under a light microscope.
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bioRxiv - Plant Biology 2020Quote: ... Polysaccharide cell wall was stained with 0.1% calcofluor white M2R (Polysciences, CAT#4359).
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bioRxiv - Molecular Biology 2020Quote: ... The cells were washed with PBS and then post-fixed in 1.5% osmium tetroxide (Polysciences) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... which was pre-incubated with 900 μl 1 mg/ml PEI MAX 40k (Polysciences, USA) for 15 min at room temperature in 20 ml Expi 293 Expression Medium ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T cells in a 10 cm dish were transfected with 40 µL 1.0 mg/mL polyethylenimine (Polysciences, Warrington, PA) and 15 µg plasmid and incubated for 24 h ...
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bioRxiv - Microbiology 2020Quote: ... using polyethyleneimine (PolySciences, Warrington, PA). VSV-G-pseudotyped viruses were produced by transfecting HEK293T cells with the pNL4-3-derived plasmids and VSV-G plasmid at a ratio of 4:1 (pNL4-3:VSV-G).
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bioRxiv - Microbiology 2020Quote: ... 2.5% glutaraldehyde (01909; Polysciences) in 0.1M cacodylate pH7.4 ...
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed in 3% paraformaldehyde and were stained for 1 hour with 1% Uvitex (Polysciences), which specifically binds to the chitin of the fungal cell wall ...
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bioRxiv - Microbiology 2020Quote: ... Transfection was done using reagent Polyethyleneimine (PEI, Polysciences). For production of virus particles ...
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bioRxiv - Microbiology 2020Quote: ... triple plasmid transfection of 293 adherent cells was performed with a polyethylenimine (PEI)-MAX (Polysciences, MW, 40,000) to DNA ratio of 3:1 (a mass-per-mass ratio) ...
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bioRxiv - Developmental Biology 2020Quote: Embryonic Müllerian and adult oviducts were dissected in PBS and fixed in 4% w/v PFA/PBS (Polysciences) for 30 minutes at room temperature followed by 3 PBS washes ...
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bioRxiv - Bioengineering 2020Quote: ... and 4 μg CD19-CAR-GFP transfer plasmid (Bloemberg et al. 2020) with 24 μg linear PEI (Polysciences Inc., Warrington, PA) for 6 hours ...
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bioRxiv - Microbiology 2020Quote: ... 293T cells were transfected with 1 mg/mL polyethylenimine (PEI; Polysciences, Warrington, PA) at a ratio of PEI-to-DNA of 3:1 ...
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bioRxiv - Cancer Biology 2020Quote: ... using polyethylenimine (Polysciences, Warrington, PA, USA; #23966-2). The medium containing lentiviral particles were collected at 48 and 72Lh after transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were bound to Concanavalin A beads (86057-3, Polysciences Inc), followed by permeabilization using a digitonin containing buffer ...
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bioRxiv - Cancer Biology 2020Quote: Lentivirus was produced in HEK293T cells by transfecting plasmids together with helper packaging plasmids (VSVG and psPAX2) using polyethylenimine (PEI 25000; Polysciences; Cat. No. 23966-1) transfection reagent ...
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bioRxiv - Cancer Biology 2020Quote: ... pVsVg and pRevRes in complex with 24μg PEI (Polysciences). After 48 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... Transfection was performed using Polyethylenimine (PEI, 25kD linear, Polysciences).
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bioRxiv - Cancer Biology 2020Quote: ... DNA was added at a final concentration of 0.675 μg/mio cells and polyethylenimine (Polysciences, #23966-1) was added to a final concentration of 0.01 mg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... Sections were washed with PBST and mounted using Aqua-Poly/Mount (Polysciences, Inc., Warrington, PA, USA).
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bioRxiv - Neuroscience 2020Quote: ... Tissue was then infiltrated with a mix of Lowicryl HM20 resin (Polysciences) and methanol (1:2 ...
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bioRxiv - Cell Biology 2020Quote: ... Purified cDNA was incorporated into a pcDNA3.1 vector and transfected into HEK293T cells using PEI max transfection reagent (Polysciences, Germany) as described previously (18) ...
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bioRxiv - Cell Biology 2020Quote: ... we used the 40 kDa linear polyethylenimine (Polysciences Europe GmbH, Hirschberg, Germany) at a 1:3 ratio (DNA ...
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bioRxiv - Cell Biology 2020Quote: ... Each plasmid and packaging vectors (pCMV-VSV-G-RSV-Rev and pCAG-HIVgp) were used to transfect HEK293T cells with Polyethylenimine MAX transfection reagent (Polysciences), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: EVs were incubated overnight (4°C) with polystyrene microspheres 4.5 µm (Polysciences INC, Warrington, PA). The microspheres were blocked with 2% BSA (v/w ...
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bioRxiv - Cell Biology 2020Quote: Polybead Amino Microsphere 3 μm latex beads (Polysciences INC) were labeled with the fluorescent dye DyLight680 mono-N-hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2020Quote: ... 900 μL 1 mg/mL PEI MAX 40k (Polysciences, 24765-1) and 300 μg DNA ...
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bioRxiv - Biophysics 2020Quote: ... Cells were transiently transfected with desired cDNAs using Polyethylenimine (1 μg/μl; Polysciences) for electrophysiological studies ...