Labshake search
Citations for Polysciences :
2801 - 2850 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... ANDV GPC or HTNV GPC (100 μg, in pCAGGS vector) and Tim-1 (6 μg) using PEI (3:1 PEI: DNA, Polysciences, Inc.). After 24 h ...
-
bioRxiv - Immunology 2020Quote: ... Plasmids were transfected using polyethylenimine (Polysciences, Inc.) into 293T cells at a ratio of 1:1.5:1 of pCMVi/pCNCG/pl.18-CVS-11 ...
-
bioRxiv - Immunology 2020Quote: ... pPPI4 DNA vectors carrying free SOSIP trimers or SOSIP-based antigen-bearing components were transfected into FreeStyle 293F cells using polyethyleneimine (Polysciences, Inc) as described previously(6) ...
-
bioRxiv - Neuroscience 2020Quote: ... Monolayer cells grown on glass coverslips were fixed in 4% methanol-free formaldehyde (Polysciences), diluted in DPBS (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were reverse transfected with 3 to 25 ng receptor vector and ~200 ng combined reporter vectors per well using polyethylenimine (Polysciences). Six h after transfection ...
-
Sequence and structural variations determining the recruitment of WNK kinases to the KLHL3 E3 ligasebioRxiv - Molecular Biology 2020Quote: ... Cells were split into 150 mm dishes and transfected with 14 μg Flag-WNK3 construct using polyethylenimine (Polysciences). Some 36 hours post-transfection ...
-
bioRxiv - Cell Biology 2020Quote: ... Plasmid transfection into HEK 293T cells was carried out using polyethylenimine (Polysciences) or Lipofectamine 2000 transfection reagent (Invitrogen) ...
-
bioRxiv - Immunology 2020Quote: ... the PolyLink Protein Coupling Kit (Polysciences) was used as indicated by the manufacturer ...
-
bioRxiv - Immunology 2020Quote: ... The vector with antigen-bearing component was transfected into FreeStyle 293F cells using polyethylenimine (Polysciences, Inc) as described previously 7 ...
-
bioRxiv - Immunology 2020Quote: ... The two plasmids expressing IgA1-Fc and J-chain were co-transfected into the cells using polyethylenimine (Polysciences). Four days after transfection ...
-
bioRxiv - Immunology 2020Quote: Mice were given a single oral gavage of 2 × 1011 of Fluoresbrite Yellow Green labelled 200 nm microbeads (Polysciences, Hirschberg an der Bergstrasse ...
-
bioRxiv - Neuroscience 2020Quote: ... and sealed using Aqua Polymount (Polysciences, Inc.). Images were captured using a laser scanning confocal microscope (LSM 880 ...
-
bioRxiv - Neuroscience 2020Quote: ... Larval stage 4 (L4) worms were mounted on 3% agarose pads and immobilized with microbeads (Polysciences) or 0.1 mM levamisole ...
-
bioRxiv - Neuroscience 2020Quote: ... and 48 μl of 2 mg/mL polyethyleneimine (PEI; Polysciences, 24765) were mixed and transfected into Lenti-X 293T (Clontech ...
-
bioRxiv - Neuroscience 2020Quote: ... and PEI (Polysciences, 24765) with Opti-MEM (Life Technologies ...
-
bioRxiv - Biochemistry 2020Quote: ... For formation of DNA:PEI complexes 1 μg DNA/mL transfection volume and 5 μg of 40 kDa PEI (Polysciences) were first diluted separately in 5% transfection volume in supplemented F17 media ...
-
bioRxiv - Biochemistry 2020Quote: ... For transfection cells were centrifuged and resuspended in fresh media to a density of 4×106 cells/mL and transfected with 4 μg plasmid/mL and 16 μg/mL of PEI 40 kDa (Polysciences). 4 h up to 24 h after transfection cells were fed with 75% of the transfection volume ...
-
bioRxiv - Biochemistry 2020Quote: ... The expression construct was transiently transfected in HEK 293F cells using polyethylenimine (Polysciences, Inc, Warrington, PA). Protein was purified from cell supernatants using Ni-NTA resin (Qiagen ...
-
bioRxiv - Neuroscience 2020Quote: Fast Blue (Polysciences, 17740-1), CM-DiI (Thermo Fisher ...
-
bioRxiv - Neuroscience 2020Quote: ... Then 5mg of Fast Blue (Polysciences), a neuronal tracer that is retained and diffuses within the lipid bilayer ...
-
bioRxiv - Biochemistry 2020Quote: ... 24 hours prior to transfection with polyethylenimine PEI (Polysciences). The primary transfection mixtures contained 250 ng pFLuc-TAR reporter plasmid ...
-
Fast and sensitive diffuse correlation spectroscopy with highly parallelized single photon detectionbioRxiv - Neuroscience 2020Quote: Our decorrelating tissue phantom was created by a 1 μm polystyrene microsphere-in-water suspension (Polysciences, inc.; Warrington, PA) in custom made cuvettes ...
-
bioRxiv - Biophysics 2020Quote: ... Transfections were performed using polyethylenimine 25k (PEI 25k, Polysciences, Inc) following manufacturer’s instructions ...
-
bioRxiv - Biophysics 2020Quote: ... 400 μg of plasmid was premixed with 1.2 mL of 1 mg/mL PEI MAX (Polysciences) in 15 mL of FreeStyle 293 expression medium ...
-
bioRxiv - Bioengineering 2020Quote: ... Green fluorescent microbeads (d ~ 25μm, Fluoresbrite, Polysciences) were mixed with 20% or 80% PEGDA prepolymer solution (MW 4000 Da ...
-
bioRxiv - Bioengineering 2020Quote: ... and 10 ng of the TetR reporter plasmid pTS1017) was added into 300 μL DMEM and mixed with 4 μg of polyethyleneimine (Polysciences Inc.). The transfection mixture was vortexed and incubated for 20 min before adding into the inner 60 wells of the cell culture plate ...
-
bioRxiv - Cancer Biology 2020Quote: ... and polyethylenimine polymer (Polysciences Inc; 23966-1), which had been pre-incubated for 15 min at room temperature ...
-
bioRxiv - Cancer Biology 2020Quote: ... HEK293T cells were seeded for 18–24 hr and transiently transfected with the indicated vectors using either the JetPrime reagent (Polyplus-transfection; 114-15) or polyethylenimine polymer (Polysciences Inc; 23966-1), following manufacturer’s instructions.
-
bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with polyethylenimine (PEI; linear, MW 25,000; Polysciences, cat. No. 23966-2) at a 2:1 ratio (PEI:DNA ...
-
bioRxiv - Microbiology 2020Quote: ... using polyethyleneimine (Polysciences). All downstream experiments were performed 24 hours post-transfection.
-
bioRxiv - Biochemistry 2020Quote: HEK293TR cells were transfected with pcDNA4/TO encoding SNAP-tagged β2AR and A2AR with PEI transfection reagent (Polysciences Europe GmbH) (PEI:DNA ratio of 3:1) ...
-
bioRxiv - Cell Biology 2020Quote: ... 5*10^6 293FT cells were transfected in 10 cm dishes using 4 μg polyethylenimine (PEI, Polysciences) per μg plasmid DNA with lentiCRISPR_V2 (Addgene 52961) ...
-
bioRxiv - Bioengineering 2020Quote: ... After washing with acetic acid (1%, Fluka) samples were incubated with safranin O (0.1%, 15 min, Polysciences) to stain proteoglycans in red ...
-
bioRxiv - Biochemistry 2020Quote: ... Tissue belts were placed into cryomolds (Peel-A-Way 22 x 30 mm molds, Polysciences # 18646B) using a stereo microscope ...
-
bioRxiv - Cell Biology 2020Quote: Transient transfections were performed using 25 kDa linear polyethylenimine (Polysciences) (Durocher et al. ...
-
bioRxiv - Developmental Biology 2020Quote: Embryos were fixed in 4% formaldehyde (Polysciences) for 10 minutes then immediately permeabilized for 30 minutes in 0.5% Triton X-100 (Millipore-Sigma ...
-
bioRxiv - Genomics 2020Quote: ... Polyethylenimine linear MW 25000 transfection grade (PEI 25K) (cat. # 239661) from Polysciences Inc. ...
-
bioRxiv - Cell Biology 2020Quote: ... 60 μl of 1 ug/ml PEI MAX (Polysciences Inc.). After 16 hours ...
-
bioRxiv - Developmental Biology 2020Quote: ... in Aqua-Poly/Mount (Polysciences, Inc (18606)).
-
bioRxiv - Developmental Biology 2020Quote: ... Embryos were mounted in AquaMount (PolySciences, Inc.) and imaged using a Zeiss LSM710 microscope with a C-Apochromat 40x water-immersion objective or a Nikon A1-RS scanning confocal microscope with 40x water-immersion objective ...
-
bioRxiv - Genetics 2020Quote: ... elegans maintained on NGM plates seeded with OP50 or subjected to feeding-based RNAi were mounted onto agarose pads (10% agarose in M9 buffer) seated on glass slides and immobilized in a slurry of 0.1 ìm polystyrene beads (Polysciences, Warrington, PA).
-
bioRxiv - Pathology 2020Quote: ... and embedded in Epon 812 epoxy resin (Polysciences, Inc., Warrington, PA) under vacuum ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were dissociated using 0.05% Trypsin with 0.53 mM EDTA (Wisent Inc., Canada) and transiently co-transfected with receptor and biosensor DNA using 25 kDa linear polyethylenimin (PEI; Polysciences Inc., USA). PEI was dissolved in water as 1 mg/mL stock solution with pH of 6.5–7.5 and kept at −20°C ...
-
bioRxiv - Synthetic Biology 2020Quote: ... cells were transfected using Polyethylenimine MAX (Polysciences Inc.) or 293fectin (Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... sections were mounted on microscopic slides using Aqua Poly/Mount (Polysciences). The following primary antibodies were used ...
-
bioRxiv - Cell Biology 2020Quote: ... Live animals were immobilized with 0.01% tetramisole and 0.1% tricaine in M9 buffer and mounted on 2% agarose pads or with 0.05 μm Polybead microspheres (Polysciences) diluted 1:2 in water and mounted on 5% agarose pads ...
-
bioRxiv - Cell Biology 2020Quote: ... and picked unanesthetized onto 10% agarose pads with 0.05 μm Polybead microspheres (Polysciences) for immobilization ...
-
bioRxiv - Bioengineering 2020Quote: 18 µg of Polyethilenimin(PolySciences) was diluted diluted with 50µl of 20mM Glutamic acid (pH4.0) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... PEI MAX (Polysciences VWR) was used at a ratio of 3 µL PEI per 1 µg DNA ...
-
bioRxiv - Microbiology 2020Quote: ... Each expression vector was transiently transfected into Expi293 cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...