Labshake search
Citations for Polysciences :
2351 - 2400 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2021Quote: ... Transfection was performed using linear polyethylenimine (PEI MAX; MW 40,000; Polysciences) and a DNA:PEI ratio of 1:3 (w/w) ...
-
bioRxiv - Physiology 2021Quote: ... Cells were plated in 35-mm tissue culture treated dishes and transfected 1-2 days later using polyethylenimine 25k (Polysciences, Inc) with a mass ratio of 1:3 (cDNA:PEI) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Linear 25 kDa polyethyleneimine (PEI) (Polysciences Inc.) was prepared in phosphate-buffered saline (PBS ...
-
bioRxiv - Molecular Biology 2021Quote: ... 500,000 OSCs were bound to 10 µl Concanavalin A-coated magnetic beads (Polysciences, #86057-3) and lysed using Dig-wash buffer (20 mM HEPES pH 7.3 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Collagen staining was performed using picrosirius red as per the manufacturer’s instructions (Polysciences Inc.), and fibrous cap thickness was quantified from 3 distinct regions of the plaque and scored per lesion size as previously described [11] ...
-
bioRxiv - Molecular Biology 2021Quote: ... Sections were cover-slipped with Mowiol (Polysciences, Warrington, PA, USA) containing DABCO (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2021Quote: ... Sections were cover-slipped with Mowiol (Polysciences, Warrington, PA, USA) containing DABCO (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... postfixed in 1% OsO4 (Polysciences) in 0.1M cacodylate buffer for 30 min ...
-
bioRxiv - Microbiology 2021Quote: ... Standard calibration beads (102.7±1.3 nm) (Polysciences) were used to confirm the range of linearity of the instrument ...
-
bioRxiv - Cancer Biology 2021Quote: All lentiviruses were produced by co-transfecting lentiviral backbones with packaging vectors (delta8.2 and VSV-G) into 293T cells using PEI (Polysciences). The viral-containing supernatant was collected at 48- and 72-hours post-transfection ...
-
bioRxiv - Biophysics 2021Quote: ... For traction force microscope gels were manufactured as above by additioning 0.2 μm fluorescent beads (1:125; Polysciences). Gels were washed in 70% Ethanol and rinsed extensively in PBS after which transfected cells were plated ...
-
bioRxiv - Bioengineering 2021Quote: ... were produced as previously described using a PEI (polyethylenimine, Polysciences, 24765-2) transfection protocol followed by iodixanol gradient purification ...
-
bioRxiv - Microbiology 2021Quote: ... These samples were washed in phosphate buffer and post-fixed in 1% osmium tetroxide (Polysciences Inc.) for 1 hour ...
-
bioRxiv - Microbiology 2021Quote: ... one half of the ovaries of each of 6 crickets was fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, USA) in 100 mM phosphate buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... and then mixed 1:1 with 0.2 M sodium carbonate–bicarbonate buffer (24095, Polysciences) containing 1 M sodium chloride (S671-3 ...
-
bioRxiv - Immunology 2021Quote: ... Two vectors containing paired TCRα and β chains were transfected together into Phoenix packaging cells using PEI MAX (Polysciences). Supernatant containing retroviruses was used for infection into mouse T cell hybridoma with an NFAT-GFP reporter gene (Matsumoto et al. ...
-
bioRxiv - Immunology 2021Quote: ... HEK293T cells were transfected with the appropriate SARS-CoV-2 S gene expression vector (wild type or mutant) in conjunction with p8.9138 and pCSFLW39 using polyethylenimine (PEI, Polysciences, Warrington, USA). HIV (SARS-CoV-2 ...
-
bioRxiv - Microbiology 2021Quote: ... 5×105 cells per well in 6-well plates were transfected with 2 μg of plasmid using 8 μg of polyethylenimine (23966, Polysciences, Inc., Warrington, PA), which was reconstituted at 1 mg/mL ...
-
bioRxiv - Genomics 2021Quote: ... Concanavalin A beads (Polysciences, 25 μL bead slurry/sample) were washed twice with ice-cold Binding buffer (20mM HEPES-KOH ...
-
bioRxiv - Genomics 2021Quote: Lentivirus was produced by polyethylenimine linear MW 25000 (Polysciences 23966) transfection of HEK293FT cells with the transfer plasmid containing a Cas9 effector ...
-
bioRxiv - Genomics 2021Quote: ... using PEI transfection reagent (Polysciences), and reduced serum medium Opti-MEM (ThermoFisher Scientific) ...
-
bioRxiv - Genomics 2021Quote: ... III and undifferentiated collagens using the picrosirius stain kit (Polysciences, Inc., Pennsylvania, USA). The observer was blinded to the sample identification to avoid subconscious bias ...
-
bioRxiv - Neuroscience 2021Quote: ... or Aqua-Poly/Mount (Polysciences). For high resolution confocal laser scanning microscopy (CLSM ...
-
bioRxiv - Neuroscience 2021Quote: ... then sections were mounted on glass slides with Aqua-Poly/Mount (Polysciences). Immunofluorescence was analyzed using a Nikon Eclipse Ti-E inverted microscope (Nikon Instruments Europe B.V. ...
-
bioRxiv - Genomics 2021Quote: ... and 2 μm-diameter fluorescent beads were added to each sample as an internal standard (Polysciences, Warminster, PA, USA). The samples were analyzed on a FACSCanto™ II Flow Cytometry Analyzer Systems (BD Biosciences) ...
-
bioRxiv - Genetics 2021Quote: ... or PEI MAX (Polysciences, 24765-1) according to manufacturer instructions.
-
bioRxiv - Genetics 2021Quote: ... expression plasmids into HEK293T cells using a 1mg/mL PEI solution (Polysciences). We collected lentiviral supernatants at 48 hours and 72 hours after transfection and concentrated lentiviruses by ultracentrifugation for 120 minutes at 19,500 rpm using a Beckman SW28 ultracentrifuge rotor at 4°C.
-
bioRxiv - Cell Biology 2021Quote: ... 60% confluent HEK293T cells were transfected using PEI (Polysciences, 23966) in OptiMEM (Thermo ...
-
bioRxiv - Biochemistry 2021Quote: ... HEK293 Freestyle™ were transiently transfected at a density of 2.5 106 cells/mL in 100mL Freestyle medium (Thermo-Fisher) by addition of 150 μg plasmid and 1.8 mL of linear polyethylenimine (PEI, 0.5 mg/ml) (Polysciences). After 24h ...
-
bioRxiv - Immunology 2021Quote: ... cells using PEI (Linear Polyethylenimine with molecular weight of 25000; Polysciences Inc.). After 2∼3 days ...
-
bioRxiv - Cell Biology 2021Quote: ... The tissue was frozen in OCT compound in an embedding mold (Polysciences, Inc.) in liquid nitrogen and stored at –80°C ...
-
bioRxiv - Cell Biology 2021Quote: ... gag-pol and the plasmid of interest in PEI (2μg per μg of DNA; Polysciences Inc) and Opti-MEM (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... Dissected midguts were fixed in 4% Formaldehyde (Polysciences) in 1x PBS for 30 minutes at room temperature ...
-
bioRxiv - Physiology 2021Quote: ... The 4-μm bone sections were stained with the von Kossa method and counterstained with tetrachrome (Polysciences Inc., Warrington, PA, USA). The 8-μm sections remained unstained to measure fluorochrome-based indices of bone formation.
-
bioRxiv - Physiology 2021Quote: ... Cells were transfected with Polyethylenimine-PEI Transfection Reagent (Polysciences, 23966-2) carrying the LC3-eGFP plasmid (60 ...
-
bioRxiv - Microbiology 2021Quote: ... The re-circularized genome was transfected into 293TT cells with PEI max transfection reagent (Polysciences, 24765-1). Progeny viral particles were purified on a linear cesium chloride gradient and titered as described previously (27 ...
-
bioRxiv - Cell Biology 2021Quote: ... PEImax (Polysciences) or the Amaxa Cell Line Nucleofector Kit V (Lonza VCA- 1003 ...
-
bioRxiv - Physiology 2021Quote: ... and stained using Picrosirius red (Polysciences, Inc) as previously described (29) ...
-
bioRxiv - Biochemistry 2021Quote: ... The expression construct was transiently transfected in HEK 293T cells using polyethylenimine (Polysciences, Inc, Warrington, PA). Protein was purified from cell supernatants using Ni-NTA resin (Qiagen ...
-
bioRxiv - Biochemistry 2021Quote: ... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
-
bioRxiv - Microbiology 2021Quote: ... and stained with 1% aqueous uranyl acetate (Polysciences, Inc, Warrington, PA). Stained grids were imaged under Talos120C transmission electron microscope (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... and packaging plasmids (pAAV-RC1 and/or pAAV-RC2) with polyethylenimine (PEI Max; Polysciences, Warrington, USA). For production of AAV2.1-A or AAV2.1-B vector ...
-
bioRxiv - Immunology 2021Quote: ... Small-scale vector preparations were generated by polyethylenimine or PEI (Polysciences, Cat #24765-2) triple transfection of AC1 or AC3 ITR-flanked transgene ...
-
bioRxiv - Physiology 2021Quote: ... Samples were washed in PBS and mounted in aqua-poly/mount (Polysciences) or ProLong Gold antifade mounting medium (Invitrogen).
-
bioRxiv - Cell Biology 2021Quote: ... 100 μl of polyethylenimine (PEI) (Polysciences Inc.) at 1 mg/mL was added to 1 mL Optimem media (Gibco) ...
-
bioRxiv - Cell Biology 2021Quote: ... Polyethylenimine (PEI, 25 kDa linear, Polysciences) mediated transfections were carried out using a DNA to PEI ratio of 1:2.5 (w/w) ...
-
bioRxiv - Cell Biology 2021Quote: ... Glutaraldehyde (8% wt/vol; EM grade) was purchased from Polysciences.
-
bioRxiv - Cell Biology 2021Quote: ... Polystyrene microspheres (Microspheres 0.10 μm, Polysciences, Inc.) were added to the agar solution before specimen mounting to immobilize the living worms.
-
bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
-
bioRxiv - Microbiology 2021Quote: ... were transfected with 1 μg DNA/ml cells in a 3:2 (LC:HC) ratio were transfected using polyethylenimine HCl MAX (Polysciences). After 4 to 5 days of expression ...