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Citations for Polysciences :
2501 - 2550 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 0.5µm (Polysciences) were coated with Protein L (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... fluorescent carboxylate microspheres 0.5µm (Polysciences) were coated with protein L and polyclonal IgA or IgG and were incubated with neutrophils at a 500:1 ratio at 37°C for 15 minutes with gentle mixing ...
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bioRxiv - Microbiology 2021Quote: ... or Polyethylenimine “Max” (24765-1; Polysciences, Inc., Warrington, PA, USA).
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bioRxiv - Microbiology 2021Quote: Parasites were fixed in 4% paraformaldehyde/0.05% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM PIPES/0.5 mM MgCl2 ...
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bioRxiv - Immunology 2021Quote: ... HEK293A cells were transfected with equal amounts of the heavy and the corresponding light chain plasmid using linear PEI (Polysciences Cat# 23966). The media was changed after 24 h to BASAL media (50% DMEM 12430 ...
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bioRxiv - Immunology 2021Quote: ... mixed with 42 μg PEI transfection reagent (Polysciences, Inc.) and suspended in 0.5 ml diluent (10 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45 μM filter and stored at −70 °C.
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bioRxiv - Immunology 2021Quote: ... The plasmids of 9μg pHAGE-luciferase-GFP、 psPAX2 and Zhou-COVID-19-Spike were co-transfected into HEK293T cells by using 1 μg/mL polyetherimide (Polysciences, Warrington, PA) in DMEM medium containing 10% FCS ...
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bioRxiv - Immunology 2021Quote: ... Three hundred microliters of PEI (Polysciences Inc.) (1 mg/ml ...
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bioRxiv - Immunology 2021Quote: ... with Polyethylenimine “Max” (Mw 40,000; Polysciences, Warrington, PA). Virus-containing media were harvested 48 hrs after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... The entire intestine was dissected out and fixed in 1× PBS plus 8% EM-grade paraformaldehyde (Polysciences) for 1 h ...
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bioRxiv - Immunology 2021Quote: ... fixed 18 hrs later with 3% formaldehyde (Polysciences) in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were incubated in 50:50 mixture of methanol and JB4 resin (Polysciences, 00226-1, GMBH, Germany) overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... and samples were not post-fixed and were mounted in AquaPoly Mount (Polysciences, Inc.).
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bioRxiv - Immunology 2021Quote: ... HEK293F were grown to a density of ∼1.0 × 106 cells/ml and were co-transfected with heavy chain (HC) and (LC) plasmids (1:1) mixed with PEIMAX (Polysciences). After five days ...
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bioRxiv - Immunology 2021Quote: COS-1 cells were transfected with AcGFP-CDC42 using PEI MAX (Polysciences). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2021Quote: Testes and epididymides were fixed in Bouin’s solution (Polysciences Inc., Warrington, PA) and embedded in paraffin wax ...
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bioRxiv - Cell Biology 2021Quote: ... cells were fixed in 4% paraformaldehyde/0.05% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100mM PIPES/0.5mM MgCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... transfection mixtures were prepared by mixing 20 μl Polyethyleneimine MAX (Polysciences Inc, Warrington, PA) prepared at 1 mg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T or GP2-293 cells were used for lentivirus or retrovirus production using polyethyleneimine (PEI 25000, Polysciences). Supernatants containing lentiviral and/or retroviral particles were applied to RPE1-Tet3G-derived host cells for 24h ...
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bioRxiv - Genomics 2021Quote: ... 2 dishes each were transfected per condition with 38 µg of plasmid DNA using 90 µl of linear polyethyleneimine (PEI) (Polysciences Warrington, PA, USA) and incubated for 24 hours ...
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bioRxiv - Genomics 2021Quote: ... or Polyethylenimine (Polysciences, Inc.) with or without ViralBoost Reagent (Alstem ...
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bioRxiv - Genomics 2021Quote: ... was inserted lentivirally: Cas9-blastR and 3rd generation lentiviral components were transfected into HEK293T cells using PEI (Polysciences). Supernatant was harvested ...
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bioRxiv - Cell Biology 2021Quote: ... 293GP2 cells were transfected using linear 25-kDa polyethylenimine (PEI, Polysciences, Inc.), as described previously [34].
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bioRxiv - Cell Biology 2021Quote: ... with bacmid DNA complexed using linear polyethylenimine (PEI MAX, 40,000 MW, Polysciences Inc., PA) at a 12:1 (PEI ...
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bioRxiv - Cell Biology 2021Quote: ... 12 µl PEI transfection reagent (Polysciences, 24765) was added ...
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bioRxiv - Cell Biology 2021Quote: Frozen cells were freeze-substituted into Lowicryl HM20 resin (Polysciences Inc., 15924-1) using freeze-substitution device (Leica EM AFS2 ...
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bioRxiv - Bioengineering 2021Quote: ... 2-aminoethyl methacrylate (AEMA, 3.04 g, Polysciences) (molar ratio of NHS:EDC:AEMA = 1:2:1 ...
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bioRxiv - Immunology 2021Quote: ... or PEI max (Polysciences) respectively according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Cells were transfected using PEI MAX (Polysciences) at a DNA:PEI ratio of 1:3.75 ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were mounted using 10% (wt/vol) Mowiol 4-88 (Polysciences). Epifluorescence images were captured using a Nikon Eclipse NiE (40 ×/0.75 Plan Apo Nikon objective ...
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bioRxiv - Molecular Biology 2021Quote: ... OSCs were harvested and 500.000 OSCs were washed three times and coupled to 10 µl of activated Concanavalin A-coated magnetic beads (Polysciences, #86057-3) per sample ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were mounted on slides with Aqua Polymount (Polysciences). Images were acquired using a confocal laser-scanning microscope (Zeiss LSM 510 Meta ...
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bioRxiv - Neuroscience 2021Quote: ... immediately mounted under glass coverslips with Aqua-Poly/Mount (Polysciences, Warrington, PA), and imaged as described below ...
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bioRxiv - Neuroscience 2021Quote: ... as a paralyzing drug agent or with polystyrene beads (Polysciences 00876-15) of diameter 0.1 μm as friction enhancing agent on 5% agarose pads mountedwith Corning cover glass (cat No ...
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bioRxiv - Neuroscience 2021Quote: ... Cultured cell lines were transfected with plasmids using PEI MAX (Polysciences) complexes with a DNA to PEI MAX ratio of 1:3 w/w as previously described49 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were mounted on slides with Aqua Polymount (Polysciences).
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bioRxiv - Cancer Biology 2021Quote: ... Reservoirs were packed with drugs mixed with Polyethylene glycol (PEG, MW 1450, Polysciences) polymer at the concentrations indicated in Table S1 ...
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bioRxiv - Neuroscience 2021Quote: ... They were embedded in Spurr resin (Polysciences Inc., Warrington, PA, USA), cut into ultrathin sections ...
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bioRxiv - Developmental Biology 2021Quote: ... ovaries were fixed in 4% formaldehyde (Polysciences, Inc., Warrington, PA, USA) in 0.1 M potassium phosphate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... 20µl of cells were spotted onto each well of a Teflon coated 10 well slide (Polysciences, 18357) that had been pre-treated with 10ng/µl poly-L-lysine ...
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bioRxiv - Plant Biology 2020Quote: Microbead binding- and transport assays were performed analogous to previous descriptions.(Bloodgood et al., 2019) Monodisperse polystyrene microspheres (0.7 μm diameter) were purchased from Polysciences, Inc ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then transfected with 2 µg of GFP-tagged construct and 2 µg of mCherry using a 3:1 ratio of PEI MAX (Polysciences) in Opti-MEM (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... and co-transfected with the two IgG plasmids expressing the corresponding HC and LC in a 1:1 ratio at a density of 0.8-1.2 million cells/mL in a 1:3 ratio with 1 mg/L PEImax (Polysciences). The recombinant IgG antibodies were isolated from the cell supernatant after five days as described previously (20 ...
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bioRxiv - Microbiology 2020Quote: ... Samples were FS-processed and embedded in Lowicryl HM20 resin (Polysciences, USA) according to a modified protocol of Kukulski et al (Kukulski et al. ...
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bioRxiv - Microbiology 2020Quote: ... The fixed suspension of bacteria was washed in sodium cacodylate buffer and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... A 1 mL sample of exponential phase bacteria was pelleted at 6,000 rcf and resuspended in 1 mL fix (2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer ...
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bioRxiv - Microbiology 2020Quote: ... Transfection of EXPI293F cells was performed using PEI (Polyethylenimine HCl MAX; Polysciences). After 4 to 6 days of transfection ...
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bioRxiv - Biochemistry 2020Quote: ... ADP-Glo kinase assays and liposome sedimentation assays were expressed in HEK293-GnTI suspension cells by using the polyethylenimine (Polysciences) transfection system ...
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bioRxiv - Biochemistry 2020Quote: ... VPS15 and BECN1 constructs were transfected to cells using polyethylenimine (Polysciences). After 60 h expression ...