Labshake search
Citations for Polysciences :
2451 - 2500 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... Sections were mounted with glass coverslips using Aqua Polymount (Polysciences, 18606-5). Images were taken using a Leica SP8 confocal microscope or a Keyance fluorescence microscope and processed using ImageJ (Fiji) ...
-
bioRxiv - Biophysics 2021Quote: ... The plasmid constructs were transfected into cells using polyethyleneimine (Polysciences Inc.) (Baldi et al. ...
-
bioRxiv - Pathology 2021Quote: ... PolyFreeze tissue freezing medium (Polysciences 19636-1) was used to cryopreserve pieces ...
-
bioRxiv - Cancer Biology 2021Quote: ... and HAP1 cells were initially tested for absence of mycoplasma contamination (UC Berkeley Cell Culture facility) by fluorescence microscopy of methanol fixed and Hoechst 33258 (Polysciences, #09460) stained samples ...
-
bioRxiv - Developmental Biology 2021Quote: ... elegans larvae were mounted on a 10% agarose pad in a solution of 100 nm polystyrene microbeads (Polysciences Inc.) to stop worm movement ...
-
bioRxiv - Developmental Biology 2021Quote: ... and mounted in Aqua-Polymount (Polysciences; Cat#18606-100). For Avidin and Biotin-HRP based staining reactions ...
-
bioRxiv - Developmental Biology 2021Quote: ... 20µL of Concanavalin A beads (Polysciences) per sample were resuspended in 1mL of Binding Buffer (20mM HEPES-KOH ...
-
bioRxiv - Biophysics 2021Quote: ... Transfection solution was prepared by combining 20 μL (per dish, hereafter) of polyethylenimine solution (Polysciences; 1 mg/mL) and a plasmid mixture consisting of 500 ng Lg-βarr1 or Lg-βarr2 and 1μg μOR-Sm in 1 mL of Opti-MEM (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2021Quote: ... according to the standard Bac-to-Bac protocol or linear polyethylenimidine (PEI MAX; Polysciences) (Scholz and Suppmann ...
-
bioRxiv - Cell Biology 2021Quote: ... or into a bead slurry containing 20 µm polystyrene beads (Polysciences - #18329-5) in M9 + 0.5% (w/v ...
-
bioRxiv - Bioengineering 2021Quote: ... and then transfected with 0.75 μg of an equimolar mixture of the three third-generation packaging plasmids (pMD2.G, psPAX2, pMDLg/pRRE) and 0.75 μg of LSR vectors using 10 μl of polyethylenimine (PEI, Polysciences #23966) and 200 μl of cold serum free DMEM ...
-
bioRxiv - Biophysics 2021Quote: ... ~2 mg plasmids premixed with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...
-
bioRxiv - Cell Biology 2021Quote: ... Transfection was performed using PEI (Polysciences, Inc.) for HEK293T ...
-
bioRxiv - Cell Biology 2021Quote: ... Constructs were transfected to cells using polyethylenimine (Polysciences). After 60 h of expression ...
-
bioRxiv - Biophysics 2021Quote: ... and Lowicryl HM20 (Polysciences Inc, USA). Composition of each resin is given in Supplementary Methods.
-
bioRxiv - Microbiology 2021Quote: ... Samples were washed in sodium cacodylate buffer and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 hr at room temperature ...
-
bioRxiv - Genetics 2021Quote: ... and coverslips were applied using Aqua Polymount (Polysciences, Inc., Warrington, PA, USA).
-
bioRxiv - Cancer Biology 2021Quote: ... and subsequently fixed on the plate in 4% formaldehyde (Polysciences, Inc) for 30-40 minutes ...
-
bioRxiv - Cancer Biology 2021Quote: ... at a ratio of 2:2:1 using polyethylenimine (Polysciences, Warrington, PA). After 18 hours of transduction ...
-
bioRxiv - Microbiology 2021Quote: ... into Vero cell using PEI Max (MW 40,000) (Polysciences) and selected with 2.5 µg/ml Puromycin (Gibco) ...
-
bioRxiv - Cancer Biology 2021Quote: For stable cell line generation HEK293-FT cells (4×106 in a 10 cm dish) were transfected using 4µg polyethylenimine (PEI, Polysciences) per µg plasmid DNA with the LentiCRISPRv2-puro (Addgene #52961 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... BHK cells were seeded and transfected the next day with 100ng of plasmid encoding hACE2 or an empty vector using polyethylenimine (Polysciences). VSV plasmids were generated and transfected onto 293T cells to produce seed particles as previously described [8] ...
-
bioRxiv - Microbiology 2021Quote: ... cells were transfected at a cell density of 1 million mL-1 using a mixture of 1 μg of recombinant expression plasmid and 3 μg of polyethyleneimine (PEI; Polysciences, Germany). Cultures were supplemented with 0.6% Primatone RL (Merck ...
-
bioRxiv - Neuroscience 2021Quote: ... mounted with Aqua-Poly/Mount (Polysciences Inc.), and coverslipped (Fisher) ...
-
bioRxiv - Neuroscience 2021Quote: ... and pHelper) were mixed with the polyethylenimine MAX transfection reagent (PEI-MAX; Polysciences, Warrington, PA). The mixture was diluted with Opti-MEM I medium (Life Technologies ...
-
bioRxiv - Immunology 2021Quote: ... 1 μg of 25,000-molecular-weight polyethylenimine (PEI; PolySciences Inc.) in 66.67 μL Opti-MEM cell culture medium (Gibco) ...
-
bioRxiv - Immunology 2021Quote: ... 1.4 µg of WT-SARS-CoV-2 spike plasmid (2) and 60 µg of PEI-Max (Polysciences). Supernatants were harvested 48h later ...
-
bioRxiv - Immunology 2021Quote: ... 480μg of each plasmid was co-transfected with 120μg of BirA and 12mg PEI-Max (Polysciences) in the presence of 200μM biotin (final concentration) ...
-
bioRxiv - Microbiology 2021Quote: ... were seeded in 24-well plates and transfected the following day using PEI MAX (3:1 PEI to DNA ratio; Polysciences) with 500 ng pHIV-1 and 0-250 ng pcDNA3.1 protein expression construct ...
-
bioRxiv - Immunology 2021Quote: ... using PEIMAX transfection reagent (Polysciences). Transfections were incubated at 220 r.p.m. ...
-
bioRxiv - Immunology 2021Quote: ... Plasmid delivery in HEK-TLR4 cells was done using linear polyethyleneimine (PEI) 25K (Polysciences) complexed with DNA in a 2:1 ratio respectively for 24 h.
-
bioRxiv - Immunology 2021Quote: 1200 synchronized L1 worms were mixed with 6 μl fluorescent beads (Fluoresbrite Polychromatic Red Microspheres, Polysciences Inc.). 25 μl 10X concentrated OP50-1 bacteria and M9 to bring the total volume to 300 ul ...
-
bioRxiv - Immunology 2021Quote: ... using PEI Max 40K (1mg/mL, linear polyethylenimine hydrochloride, Polysciences, Inc.) at a 3:1 ratio (PEI max:DNA) ...
-
bioRxiv - Immunology 2021Quote: ... Cells were transfected using 90 mg of PEI-Max (1 mg/mL, Polysciences) with ...
-
bioRxiv - Immunology 2021Quote: ... Polybead polystyrene nonfluorescent microspheres (15 mm, Polysciences) were used to determine absolute cell numbers in flow cytometry samples ...
-
bioRxiv - Immunology 2021Quote: ... The cells were transiently transfected with polyethylenimine (Polysciences) and respective vector plasmids in Opti-MEM and FreeStyle 293 medium (Gibco ...
-
bioRxiv - Immunology 2021Quote: ... followed by counterstaining with 50% Gill’s hematoxylin #1 (Polysciences, cat # 24242-1000). Slides were viewed using Olympus IX 71 (Olympus ...
-
bioRxiv - Immunology 2021Quote: ... transient transfections of 5 µg of pcDNA3.1-based vectors were performed using 25 µg of PEI MAX (Polysciences, Warrington, PA). After an additional incubation for 24 h ...
-
bioRxiv - Molecular Biology 2021Quote: ... 3 µg of DNA diluted in 100 µL of NaCl 150 mM was mixed with 6 µL of PEI Max 40000 (Polysciences) at 2 mg/mL diluted in 100 µL of NaCl 150 mM ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1011 of 200-nm diameter polystyrene nanoparticles (Fluoresbrite YG; Polysciences) were orally administered via oral gavage ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 μg of plasmid DNA was incubated with 100 μg/mL polyethyleneimine (Polysciences Inc; linear 25 kDa) in 500 μL DMEM without serum or antibiotic for 20 minutes at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: ... PEI-MAX (Polysciences, Inc. cat# 24765) or Lipofectamine 3000 (Invitrogen in Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with 1.25 μg/well of the reporter plasmids by polyethyleneimine reagent (Polysciences, #23966) and differentiated in DMEM/10% FBS with 1µM rosiglitazone for two days ...
-
bioRxiv - Biochemistry 2021Quote: ... HeLa cells were transfected with pMQ gp120/gp160 or HA constructs using polyethylenimine (Polysciences) as described before (Hoelen et al. ...
-
bioRxiv - Immunology 2021Quote: ... The cells were incubated at 37°C for 3 days and then fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
-
bioRxiv - Immunology 2021Quote: ... Cells were transfected using 60 μg of PEI-Max (Polysciences) with a mix of three plasmids ...
-
bioRxiv - Microbiology 2021Quote: ... Four hundred μl of undiluted culture was then mixed with 50 μl of a 1:100 dilution of 1.75 μm fluorescent microspheres (Polysciences 19392). Four hundred fifty μl of the bead/culture suspension was added to the epithelial surface of individual tissue sections ...
-
bioRxiv - Immunology 2021Quote: ... by co-transfecting heavy chain and light chain expressing plasmids using polyethylenimine (PEI, Linear, MV∼25,000, Polysciences, Inc. Cat. No. 23966) and culture in 37.0C degree shaker at 125RPM and 8% CO2 ...
-
bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293™ cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
-
bioRxiv - Immunology 2021Quote: ... using polyethyleneimine (Polysciences). Media was harvested 4 days after transfection and the secreted protein purified using Ni-NTA IMAC Sepharose 6 Fast Flow resin (GE Healthcare ...