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Citations for Polysciences :
2601 - 2650 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... nuclei were extracted with nuclear extraction buffer and added to concanavalin A beads slurries (Polysciences). After blocking ...
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bioRxiv - Neuroscience 2020Quote: ... fixed in 4% Formaldehyde (Polysciences 18814) / PEM buffer (0.1 M PIPES ...
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bioRxiv - Microbiology 2020Quote: ... to 0.8×10e6 cells/ml were transfected with linear PEI (Polysciences) with 397ug DNA at a 1:3 DNA:PEI ratio ...
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bioRxiv - Biochemistry 2020Quote: ... 500 ng of plasmid and 3 ¼g of polyethylenimine (MW 25,000; Polysciences) were mixed in 100 ¼ of OptiMEM (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: Plasmid transfections were conducted by mixing DMEM and PEI-Max (Polysciences) at room temperature and incubating for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... and linear PEI (Polysciences) were diluted in 400 μl OPTI-MEM with total 5 ug of the expression plasmids ...
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bioRxiv - Molecular Biology 2020Quote: ... pSRP and p8.91-MB based plasmids with the polyethylenimine method following the manufacturer’s instructions (PEI, MW 25000, linear; Polysciences, Warrington, PA, USA). HEK 293T were seeded at 5 × 106 per 100-mm diameter dish and transfected 16-20h later ...
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bioRxiv - Molecular Biology 2020Quote: ... 10,000 photons were recorded after measurement of the internal response function with Polybead amino 0.10 micron microspheres (Polysciences, Warrington, PA, USA). Data was analyzed with version 3.0.0.80 of the Quantaurus-Tau software.
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bioRxiv - Molecular Biology 2020Quote: ... and Polyethylenimine was purchased from Polysciences. TRIzol ...
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bioRxiv - Molecular Biology 2020Quote: ... or PEI (Polysciences, Inc.) reagents as per the protocol from the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1.64 pmol respective pLKO plasmid using PEI MAX 40K (Polysciences, Inc, Warrington, PA) at a ratio of DNA to PEI 1:3 ...
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bioRxiv - Molecular Biology 2020Quote: ... and chemically fixed by re-suspending in a buffer containing 4% formaldehyde (Polysciences, Inc., Warrington, USA), 80 mM HEPES-K ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were transfected with PEI (Polysciences, #23966-2) using 15 μg of CIGAR-seq vector ...
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bioRxiv - Neuroscience 2020Quote: ... 10 µg pHelper and 6.5 µg pAAV plasmid per plate complexed with Max-polyethylenimine (PEI, Polysciences) at a PEI:DNA ratio (w/w ...
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bioRxiv - Genomics 2020Quote: ... into HEK293T cells using a 1 mg/ml PEI solution (Polysciences). Lentiviral supernatants were collected at 48 hours and 72 hours after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... polyethylenimine (PEI) (Polysciences, 23966) was used at a ratio of 1:3 (DNA:PEI) ...
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bioRxiv - Microbiology 2020Quote: ... and 0.1% uranyl acetate (Polysciences; Warrington, PA) in 2% H2O and 98% acetone in an FS-8500 freeze substitution system ...
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bioRxiv - Microbiology 2020Quote: ... Samples were infiltrated with 1:1 Polybed812 (Polysciences) resin:acetone for 24 hours ...
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bioRxiv - Biophysics 2020Quote: ... uncoated polystyrene beads (radius = 1.52 ± 0.02 µm) (Polysciences, Warrington, PA) were added and each one of the glass bottom dishes containing the cells was placed in the OT microscope ...
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bioRxiv - Biochemistry 2020Quote: ... PEI was from Polysciences. Hoechst dye was from Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: Third instar larvae and adult CNS were dissected in 1x PBS and tissue was fixed for 20 minutes in 4% formaldehyde (Polysciences, Inc., 10% methanol-free) diluted in PBST (0.3% Triton X-100 in PBS) ...
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bioRxiv - Developmental Biology 2020Quote: Budless animals were labelled with an injection needle filled with fluorescent microspheres (Flouresbrite YG Microspheres 1.00µm; Polysciences) that have been diluted 1:10 in Hydra medium prior to use ...
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bioRxiv - Biochemistry 2020Quote: ... HA-tagged constructs and bioGFP-tagged constructs using MaxPEI (Polysciences) in a ratio of 3/1 PEI/DNA ...
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bioRxiv - Synthetic Biology 2020Quote: ... The transfection solution was prepared using Polyethylenimine (PEI) “MAX” (Mw 40000; Polysciences, Inc.) in a 1:3 (ug DNA to ug PEI ...
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bioRxiv - Physiology 2020Quote: ... The retrograde tracer Fast Blue (FB) (2%; Polysciences, PA) was injected with a microsyringe into 5-10 separate loci (1 μl/locus ...
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bioRxiv - Immunology 2020Quote: ... Polyethylenimine (PEI) (linear, MW 25,000; Polysciences # 23966-2) was diluted to 60 μg/mL in PBS and incubated for at least two minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... and mounted in AquaPolymount (Polysciences, Inc.). Anti-snail (rabbit ...
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bioRxiv - Bioengineering 2020Quote: ... with three nanoparticles of interest: 100 nm diameter polystyrene nanoparticles (PNPs; Fluoresbrite® yellow-green fluorophore-labeled with ex441nm em486nm, Polysciences Inc.), (GT)15-SWCNTs ...
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bioRxiv - Cancer Biology 2020Quote: ... or 2×108 yellow/green 1μm fluoresbrite beads (Polysciences Inc.) for 20min ...
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bioRxiv - Developmental Biology 2020Quote: ... psPAX2 and pMD2.G using PEI (Polysciences, 24765-2) after reaching 70-80% confluence ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were mounted with Aqua-Poly/Mount reagent (Polysciences). Corrected total cell fluorescence (CTCF ...
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bioRxiv - Biochemistry 2020Quote: ... using 1 μg of plasmid DNA and 3 μg of linear polyethyleneimine (PEI; Polysciences, Germany). Cells were harvested 6 days after transfection by centrifuging the medium for 15 minutes at 1000 x g ...
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bioRxiv - Biochemistry 2020Quote: ... 960 μg PEI (Polyethylenimine, linear, MW∼25,000; Polysciences Inc, #23966) was added from a sterile 1 mg/ml stock prepared in 50 mM Hepes-KOH pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Sections were washed in PBS and mounted with coverslips using AquaPolymount (Polysciences). Primary antibodies included ...
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bioRxiv - Biochemistry 2020Quote: ... Transfections were performed using linear polyethylenimine (PEI MAX, Polysciences) in a 5:1 (w:w ...
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bioRxiv - Cell Biology 2020Quote: Ten million mouse embryonic fibroblasts (ATCC) were transfected with 90uL of Polyethylenimine (PEI MAX #24765 Polysciences) diluted in 240uL NaCl 150mM and 15ug of the pX335-gRNA and pUC19-homology arms-mCherry plasmids also diluted in 240uL NaCl 150mM ...
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bioRxiv - Genetics 2020Quote: ... Worms were immobilized on agar pads using polybead® polystyrene 0.10µm microspheres (Polysciences, Inc.), and pictures were taken quickly thereafter to avoid undo stress ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were embedded in Aqua-Poly/Mount (Polysciences). Confocal image acquisition was performed with an Olympus IX81 microscope combined with an Olympus FV1000 confocal laser scanning microscope ...
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bioRxiv - Genomics 2020Quote: All lentiviruses were produced by co-transfecting lentiviral backbones with packaging vectors (delta8.2 and VSV-G) into 293T cells using PEI (Polysciences). The viral-containing supernatant was collected at 48- and 72-hours post-transfection ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed twice in 1× TBS and were finally embedded in Aquapolymount (Polysciences). Positive control for the primary antibody directed against Nestin was kidney tissue ...
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bioRxiv - Neuroscience 2020Quote: ... vaginal smears were stained with Giemsa solution (Polysciences inc.) (Caligioni 2009 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were mounted in Aqua-Poly-Mount (Polysciences).
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bioRxiv - Neuroscience 2020Quote: ... and pcDNA3-RTP1s (25 ng/well) using PEI Max (Polysciences, Inc., #24765-1). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... was added in PBST before the last washing step and the samples were mounted with Aqua/Poly Mount solution (Polysciences Inc., US).
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bioRxiv - Cell Biology 2020Quote: ... was added in PBST before the last washing step and the samples were mounted with Aqua/Poly Mount solution (Polysciences Inc., US). Origins and diluted concentrations of all buffers and antibodies are noted in Table 1 ...
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bioRxiv - Neuroscience 2020Quote: ... the brains were embedded in O.C.T Tissue-Tek (Sakura) in Peel-a-Way® disposable embedding molds (Polysciences), frozen in isopentane on dry ice and cut on a cryocut (CM1850 ...
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bioRxiv - Neuroscience 2020Quote: ... Finally sections were subsequently washed with PBS (3 x 10 min) and mounted in Aqua/polymount (Polysciences, 18606).
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed trice with PBS and mounted using Aqua Polymount solution (Polysciences). Cells were visualized using fluorescent confocal microscope (Leica ...
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bioRxiv - Neuroscience 2020Quote: ... and negatively stained with 0.5% uranyl acetate (Polysciences). The grids were imaged in a FEI Tecnai F20 electron microscope (Eindhoven ...
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bioRxiv - Cell Biology 2020Quote: Polystyrene microspheres (Polysciences, Eppelheim, Germany) were coated with GST-pWA60 ...