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Citations for Polysciences :
2301 - 2350 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Mice were sacrificed at 2 and 10 months of age by perfusion with 4% w/v paraformaldehyde (PFA; Polysciences, EM grade) and 0.1% v/v glutaraldehyde (GA ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected at 80% confluency in whole medium employing the transfection reagent polyethyleneimine (Polysciences Europe GmbH, Germany). The cells were lysed using 50 mM Tris-HCl (pH 7.3) ...
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bioRxiv - Neuroscience 2021Quote: ... temporal overlapping calibration of the pump and Stokes beams at the fingerprint and C-H vibration regions was performed by adjusting a built-in delay stage based on the SRS signal of 6 μm polystyrene beads (Polysciences, Inc., Warrington, PA), Olive oil and heavy water (99% pure ...
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bioRxiv - Microbiology 2021Quote: ... the pLHCX or pQXCIP packaging vector of interest and pCMV VSV-G using 1mg/ml PEI®-MAX (Polysciences). Media was replaced 16 h post-transfection and viral supernatants were harvested through a 0.45 µm filter ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transfected using 1 mg/ml PEI MAX® (Polysciences). Media was replaced 16 h post-transfection and viral supernatants were harvested through a 0.45µm filter 48 h post transfection ...
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bioRxiv - Neuroscience 2021Quote: ... or 5 µl of 2% Fast-Blue (Polysciences, Warrington, PA, USA). Tracer leakage was prevented by sealing the reservoir around the nerve with Vaseline (Vaselinum album ...
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bioRxiv - Microbiology 2021Quote: ... spores were harvested in KK2 buffer and fixed with 2% paraformaldehyde with 2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM cacodylate buffer (pH 7.2 ...
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bioRxiv - Microbiology 2021Quote: ... We washed samples with cacodylate buffer and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 hr before rinsing in dH2O prior to en bloc staining with 1% aqueous uranyl acetate (Ted Pella Inc. ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA (1 mg mL-1) was mixed with linear 25 kDa polyethyleneimine (2 mg mL-1; Polysciences) and co-transfected in OptiPRO SFM medium (4% final concentration ...
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bioRxiv - Bioengineering 2021Quote: The channel was perfused with a suspension of (1.7 µm-diameter microbeads (Polysciences, Inc.) in PBS (1.8×109 beads.mL-1 ...
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bioRxiv - Bioengineering 2021Quote: ... and packaging plasmids pCMV-dR8.91 (900 ng) and pCMV-VSV-G (100 ng) with 12 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences 24765-1) into HEK293T cells that had been grown to 60-80% confluence in 6-well plates ...
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bioRxiv - Microbiology 2021Quote: ... or PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected at 40-50% confluence using PEI (Polysciences Inc.) [47] and used 24 hours after transfection.
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bioRxiv - Neuroscience 2021Quote: ... in hard drying fluorescent mounting medium (PolyMount, 18606, PolySciences) at a dilution of 1:80 (beads:mounting medium) ...
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bioRxiv - Biophysics 2021Quote: ... polystyrene reference beads (Polysciences Europe) of 1.5 µm in diameter were diluted 1:1500 in PBS buffer (pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for 1 min and coverslips were mounted onto SuperFrost® Plus slides (R. Langenbrink) with fluorescent Aqua-Poly/Mount mounting medium (Polysciences, 18606-20). The cells were imaged using a Leica TCS SP5 confocal laser scanning microscope and an HCX PL APO lambda blue 63× oil UV objective or HCX PL APO 40.0×1.30 OIL objective controlled by the LAS AF scan software (Leica Microsystem ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 10% paraformaldehyde (Polysciences) for 24 hours and then stained with anti-spike antibodies and plaques were counted.
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bioRxiv - Biochemistry 2021Quote: ... ~1.8 mg pCAG-ATP8B1 and ~0.3 mg pCAG-CDC50A/B were incubated with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...
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Physiological activation of the nephron central command drives endogenous kidney tissue regenerationbioRxiv - Physiology 2021Quote: ... histological analysis of periodic acid-Schiff stain was performed on mouse kidney sections using PAS Stain Kit (24200-1, Polysciences, Warrington, PA). Images were visualized at 25× magnification using Leica TCS SP8 (Leica Microsystems ...
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bioRxiv - Cancer Biology 2021Quote: ... using 5 μL of Polyethylenimine (PEI) (Polysciences, 23966-1) at 1 mg/mL stock concentration ...
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bioRxiv - Cancer Biology 2021Quote: ... we used Rapid PTAH Stain Kit (Polysciences, Warrington, PA, USA). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 ×106 cells/mL using PEI (Polyethyleneimine “MAX”, MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 48 h ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected at a cell density of 2.5×106 cells/mL by co-transfecting plasmids (1.1 mg total DNA/L cells) using PEI (Polyethyleneimine “MAX”, MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells).
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bioRxiv - Biochemistry 2021Quote: ... Stable cell lines expressing N-terminal Flag tagged receptor constructs in pcDNA3.1 vector were generated by transfecting HEK-293 cells with 7 μg of plasmid DNA using polyethylenimine (PEI) (Polysciences, Cat. no. 19850), followed by selection using G418 (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293TM cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Biochemistry 2021Quote: ... by co-transfection heavy chain and light chain expressing plasmids using polyethylenimine (PEI, Linear, MV∼25,000, Polysciences, Inc. Cat. No. 23966) and culture in 37 °C degree shaker at 125RPM and 8% CO2.
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bioRxiv - Bioengineering 2021Quote: ... Polyethyleneimine (PEI, Polysciences) was independently diluted to 0.1 mg/mL in OptiPro medium and incubated at room temperature for 5 min ...
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bioRxiv - Biophysics 2021Quote: ... with pre- incubated expression vector (300 µg/L) and polyethyleneimine (PEI) MAX (Polysciences) (900 µg/L) ...
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bioRxiv - Biophysics 2021Quote: ... The water surrounding the spheres was seeded with 2 µm polystyrene spheres (Polysciences 19814). PIV analysis was performed using PIVlab [53] ...
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bioRxiv - Biophysics 2021Quote: ... approximately 2.0 mg plasmids (1.4 mg for Nav1.5-E1784K and 0.6 mg for β1) and 4 mg 25-kDa linear polyethylenimines (PEIs) (Polysciences) were mixed into 30 mL fresh medium and pre-incubated for 15-30 min before adding into 1 liter cell culture ...
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bioRxiv - Immunology 2021Quote: ... using polyethylenimine (Polysciences). Cells were allowed to grow in 37°C ...
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bioRxiv - Microbiology 2021Quote: ... An equal amount of Optimem containing PEImax (1 mg/ml, Polysciences, Warrington, PA, USA) in a 1:3 DNA:transfection reagent ratio was prepared and added to the overexpression plasmids ...
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bioRxiv - Cancer Biology 2021Quote: ... A Fluoresbrite YG microsphere (Polysciences Inc.) was imaged as a daily standard for fluorescence lifetime ...
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bioRxiv - Cancer Biology 2021Quote: ... 138 μl 1mg/mL PEI (Polysciences) was mixed with 862 μl Opti-MEM ...
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bioRxiv - Biophysics 2021Quote: ... Commercially available polystyrene beads (Polysciences GmbH, Germany) with a diameter of 1.5 μm were used as reference beads fixed to the glass surfaces ...
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bioRxiv - Molecular Biology 2021Quote: Plasmid DNA was transfected using polyethylenimine (PEI) (Polysciences Inc, 24765-1) or Lipofectamine 3000 (Life Tech ...
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bioRxiv - Microbiology 2021Quote: ... Optimization and calibration of the quantification parameters were achieved using yellow-green 1 μm microspheres (441/485 ex/em; Polysciences, PA). Live cyanobacteria were also quantified using Syto9 using FITC filters ...
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bioRxiv - Physiology 2021Quote: ... Immune infiltration of the β-cell was determined using standard eosin (Polysciences Cat #17269) and hematoxylin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were picked up onto 1×2 mm formvar coated copper slot grids (Polysciences), and further stained with uranyl acetate followed by lead citrate ...
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bioRxiv - Molecular Biology 2021Quote: ... (PEI Polysciences Inc.) transfection reagent ...
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bioRxiv - Neuroscience 2021Quote: ... and 70% Lowicryl HM20 resin (Polysciences Inc., Warrington, PA) in acetone for 2 h each followed by 100% HM20 resin overnight ...
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bioRxiv - Pathology 2021Quote: ... These were stained with toluidine blue (AgarScientific; Essex, United Kingdom) and basic fuchsine solution (Polysciences Inc.; Warrington, PA). Subsequently ...
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bioRxiv - Neuroscience 2021Quote: ... washed again three times for 10 min in 0.1 M PBS and coverslipped with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Microbiology 2021Quote: ... we transferred a polystyrene bead (Polysciences, Inc, Warrington, PA) to fresh media containing three sterile beads ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... PEI MAX was purchased from Polysciences Inc ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... using 108-144 μg PEI MAX 40K (Polysciences, cat. 24765; stock 1 μg /ul) mixed in 1.8 ml Opti-MEM (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells/mL culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa PEI (Polysciences, 24765-1) per mL culture volume ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl polyethylenimine (PEI) 25K (1mg/ml; Polysciences, 23966-1) per μg of plasmid DNA were mixed with pre-warmed Opti-MEM media (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Cells with a concentration of 106 cells/mL were transfected with 1.5 mg of DNA per liter of the culture using transfection reagent 40 kDa PEI MAX (Polysciences, Inc.) in a 1:2 ratio ...
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bioRxiv - Systems Biology 2021Quote: ... Firefly reporter and Renilla transfection control were transfected together with plasmids expressing viral proteins using polyethylenimine (PEI, Polysciences) for untreated and treated conditions ...