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Citations for Polysciences :
2201 - 2250 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: rAAV-DJ were produced in HEK293T cells (ATCC) by triple transient transfection using polyethylenimine (PEI, Polysciences Inc). For each vector ...
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bioRxiv - Immunology 2021Quote: ... The IgG and IgK plasmid DNA sequences were verified for the CDR3 sequences by Sanger sequencing and these plasmid pairs were diluted in PBS followed by addition of polyethyleneimine (Polysciences, Warrington, PA). After 15 mins of incubation at room temperature ...
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bioRxiv - Immunology 2021Quote: ... PEI precipitation was performed by incubating the plasmids with 420 μg PEI (molecular weight 25,000, Polysciences, Inc) in 10 mL Opti-MEM (Life technologies ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 2 mg of PEI “MAX” (Polysciences) (40 kDa ...
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bioRxiv - Microbiology 2021Quote: ... and 1 μg of pHEF-VSV-G into 70% confluent monolayers of 293T cells in a 10 cm dish using polyethylenimine (Polysciences Inc, Warrington, PA.) at 4X total transfected DNA in 800 μl 150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... into 70% confluent monolayer of 293T cells in a 10 cm dish using polyethylenimine (Polysciences Inc, Warrington, PA.) at 4X total transfected DNA in 800 μl 150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... and sorting efficiency was verified manually by sorting a specified number of 1-μm yellow-green microspheres (Polysciences, USA) onto a glass slide and counting the beads under an epifluorescence microscope ...
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bioRxiv - Microbiology 2021Quote: ... Calibration and alignment were done using 1-μm yellow-green microspheres (Polysciences, USA).
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bioRxiv - Microbiology 2021Quote: Polystyrene microspheres (FluoresbriteTM Carboxylate YG, 0.5 μm and 1 μm, (Polysciences)) were incubated on ice with 5 μM MirA for >1 h before adding BSA to a concentration of 5 mg/mL and incubating for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... pEIAV-GagPol and vesicular stomatitis virus glycoprotein (VSVG) expression plasmids using the PEI transfection reagent (Polysciences) as described previously ...
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bioRxiv - Microbiology 2021Quote: ... The fixed and stained cells were washed with PBS and mounted using Aqua-Poly/Mount (Polysciences). Confocal images of infected cells were acquired using a Leica SP8 Scanning Confocal Microscope controlled by Leica-X software ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Polyethylenimine (PEI; #NC1014320, Polysciences, Warrington, PA, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were transfected using polyethylenimine (PEI, Polysciences Inc.) and 150 mM NaCl using a 1:6 (w:v ...
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bioRxiv - Synthetic Biology 2021Quote: ... Polyethylenimine (PEI; Polysciences; 24765) was used as transfection reagent ...
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bioRxiv - Synthetic Biology 2021Quote: ... PEI MAX (Polysciences VWR) was used at a ratio of 3 μL PEI per 1 μg DNA ...
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bioRxiv - Microbiology 2021Quote: ... 1 M anti-digoxygenin coated polystyrene beads (Polysciences) were conjugated to the peptide construct through λ-DNA of the peptide construct by incubation in a buffer containing 10 mM Tris HCl pH 7.5 and 150 mM NaCl for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with PBS and incubated with cold 4% (v/v) electron microscopy-grade formaldehyde (PFA, Polysciences) in PBS for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were seeded in 35-mm glass-bottom cell-culture dishes (Iwaki) and transiently transfected with the constructed plasmid using polyethyleneimine (Polysciences). Transfected cells were imaged using a IX83 wide-field fluorescence microscopy (Olympus ...
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bioRxiv - Molecular Biology 2021Quote: Approximately 50 to 100 million J-Lat 11.1 cells per condition were collected and crosslinked with 1% formaldehyde (Polysciences inc.) in 40 ml PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 cells were reverse transfected using poly(ethyleneimine) (linear, 20 kDA; Polysciences)49 ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... were generated via polyethylenimine (PEI, Polysciences, #23966-2)-mediated transfection of human embryonic kidney 293T (HEK293T ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... and fixated using AquaPolymount (Polysciences Inc., #18606-20). See Supplementary Table 3 for primary and second antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... 293T media was replaced with 18 mL fresh media and the cells were transfected using the polyethylenimine (PEI) (1 mg/mL, Polysciences #23966-2) transfection method ...
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bioRxiv - Neuroscience 2021Quote: ... molecular weight (MW) 25,000 (Polysciences, cat. no. 23966-1) was used as the transfection reagent ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by fixation with 1% paraformaldehyde (Polysciences, Inc). For the NKG2D blocking assay ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by fixation with 1% paraformaldehyde (Polysciences, Inc). OT-1 cells were analyzed by flow cytometry using a FACSCanto II cell analyzer (Becton Dickinson ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.5) containing 1:10 volume of polystyrene beads (Polybead® Hollow Microspheres, Polysciences). The temperature controller CherryTemp (Cherry Biotech ...
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bioRxiv - Cell Biology 2021Quote: Cells were fixed with 4% paraformaldehyde (PFA) (Polysciences, Warrington, United States). To block unspecific binding sites the fixed cells were incubated with blocking buffer containing 10% normal goat or donkey serum ...
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bioRxiv - Cell Biology 2021Quote: ... dehydrated through an acetone series and embedded in resin (Polybed 812; Polysciences, Inc.). A series of ultrathin sections were cut using a Leica UCT ultramicrotome (Leica Microsystems) ...
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bioRxiv - Cell Biology 2021Quote: ... Klf4 or Oct-3/4 or Klf4 + Oct-3/4 or Klf4 + OctER using PEI (Polysciences, USA). Self-ligated empty pGEM-T vector (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... with addition of UVITEX 2B (1 μg/mL, Polysciences, Inc.) for cell wall staining ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were expressed in HEK293T cells (2 300 000 cells in 10-cm dish) transfected with 10 µg of DNA using polyethylenimine (Polysciences, # 23966-2) and 48h later ...
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bioRxiv - Cell Biology 2021Quote: Plasmid transfection was performed with Polyethylenimine (PEI, #23966-1, Polysciences) as transfection reagent at a ratio PEI/cDNA of 5:1 (w/w) ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were washed with 0.1% Triton in PBS and PBS only and mounted with Aqua-Poly/Mount (Polysciences). Cells were imaged with a Zeiss LSM 700 Axio Imager Z2 upright microscope equipped with a 40x Plan-apochromat 1.3 NA oil immersion lens or 63x Plan-apochromat 1.4 NA oil immersion lens (Carl Zeiss Micro Imaging Inc.) ...
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bioRxiv - Cell Biology 2021Quote: ... and embedded at −60°C in Lowicryl HM20 (Polysciences, Warrington, PA). Samples were polymerized at −60°C with and warmed slowly over four days ...
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bioRxiv - Cell Biology 2021Quote: ... rinsing in acetone and embedding in Lowicryl HM20 resin (Polysciences, Warrington PA, USA) (Hawes et al. ...
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bioRxiv - Biophysics 2021Quote: ... 2 μm diameter – microspheres (PolySciences Ltd., Warrington, PA, USA) were diluted to a concentration of 0.002% w/v (each ...
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bioRxiv - Biophysics 2021Quote: ... Cells were transiently transfected at a density of 3 × 106 cells/mL using linear polyethylenimine (Polysciences). 24 h following transfection ...
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bioRxiv - Biophysics 2021Quote: ... and 4uL of 1mg/mL PEI (Polyethylenimine HCl MAX Linear 40K from Polysciences Catalog No ...
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bioRxiv - Biophysics 2021Quote: 1×107 cells were seeded in 75cm2 flasks 24 h prior to transfection with Polyethylenimine (PEI 25K, Polysciences Europe GmbH, Germany) at a DNA to PEI ratio (w/w ...
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bioRxiv - Cancer Biology 2021Quote: ... Transfections were performed using linear polyethylenimine (Polysciences Inc.) at a ratio of 3 μg of PEI to 1 μg of DNA per 1.25 x 106 cells and aliquoted at 125 000 cells per well in white 96 well assay plates (Corning) ...
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bioRxiv - Cell Biology 2021Quote: ... transfection was performed with PEImax reagent (Polysciences, 24765), as previously described (Sugimoto et al. ...
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bioRxiv - Cell Biology 2021Quote: ... To facilitate the infiltration of Lowicryl HM20 (Polysciences Inc), the temperature was gradually raised to −25°C while increasing the resin concentration in acetone ...
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bioRxiv - Cell Biology 2021Quote: ... PEI (Polysciences, 23966-1) or lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were freeze-substituted in 0.1% uranyl acetate in acetone and embedded in Lowicryl HM20 (Polysciences) using the automated temperature control of an EM-AFS2 (Leica Microsystems ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were mounted with Aqua Poly/Mount (18606-20, Polysciences, Inc).
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bioRxiv - Cell Biology 2021Quote: ... Slides were mounted with Aqua Poly/Mount (18606-20, Polysciences, Inc).
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bioRxiv - Cell Biology 2021Quote: ... Slides were mounted with Aqua Poly/Mount (18606-20, Polysciences, Inc). The following primary antibodies were used ...
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bioRxiv - Cell Biology 2021Quote: ... counterstained in Mayers Hematoxylin solution (Fluka) and mounted under coverslips in Aqua Poly/Mount (18606-20, Polysciences, Inc).
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected using transfection grade Polyethylenimine (Polysciences, Cat # 23966) or lipofectamine 3000 (Thermo ...